Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,633 C→A 100% P243T (CCG→ACG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,6330CA100.0% 259.0 / NA 75P243T (CCG→ACG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/36);  total (39/36)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGC  >  NC_000913/756489‑756775
                                                                                                                                                |                                                                                                                                              
gCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGAc                                                                                                                                            <  5:191896/149‑1 (MQ=255)
  ccGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCg                                                                                                                                          >  4:167297/1‑149 (MQ=255)
    gcgcTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGc                                                                                                                                        <  7:337704/149‑1 (MQ=255)
        tACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTg                                                                                                                                    >  3:307490/1‑149 (MQ=255)
                 ggCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCgg                                                                                                                           <  3:328851/149‑1 (MQ=255)
                  gCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCgg                                                                                                                           <  7:177882/148‑1 (MQ=255)
                         gCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTg                                                                                                                   >  5:79623/1‑149 (MQ=255)
                         gCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTg                                                                                                                   <  2:342204/149‑1 (MQ=255)
                         gCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGACCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTg                                                                                                                   >  5:257977/1‑149 (MQ=255)
                               cAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCAcc                                                                                                             <  7:107710/149‑1 (MQ=255)
                                aGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCg                                                                                                            <  3:289958/149‑1 (MQ=255)
                                 gggCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCg                                                                                                            >  1:275427/1‑148 (MQ=255)
                                  ggCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGaa                                                                                                          <  8:132278/149‑1 (MQ=255)
                                   gCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAg                                                                                                         >  2:79301/1‑149 (MQ=255)
                                   gCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAg                                                                                                         <  8:192166/149‑1 (MQ=255)
                                    cGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGCCGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGCCCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAgg                                                                                                        >  4:83873/1‑149 (MQ=255)
                                       aTTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCATACTGGTCACCGAAGGTTg                                                                                                     >  4:51008/1‑149 (MQ=255)
                                        tttATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCCGGCGTACTGGTCACCGAAGGTTGc                                                                                                    >  1:81321/1‑149 (MQ=255)
                                        attATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTTCCGGTGCAGGAGTACTGGTCACCGAAGGTTGc                                                                                                    >  8:296999/2‑149 (MQ=255)
                                         ttATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGcc                                                                                                   >  3:313732/1‑149 (MQ=255)
                                            tCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgt                                                                                                 <  6:79623/148‑1 (MQ=255)
                                              aGTCCACCACCAACGCGCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATTGAATTGTGGCATTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCttggt                                                                                              <  7:296999/149‑1 (MQ=255)
                                              aGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGtt                                                                                                      <  3:195070/141‑1 (MQ=255)
                                              aGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGtt                                                                                                      <  3:199395/141‑1 (MQ=255)
                                              aGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGtt                                                                                                      >  4:199395/1‑141 (MQ=255)
                                              aGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGtt                                                                                                      >  4:195070/1‑141 (MQ=255)
                                               gtgcaccaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCg                                                                                                                                          <  7:307627/101‑1 (MQ=255)
                                               gtgcaccaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCg                                                                                                                                          >  8:307627/4‑104 (MQ=255)
                                               gTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                              >  8:94875/1‑148 (MQ=255)
                                                 ccaccaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggtg                                                                                             >  3:53105/1‑147 (MQ=255)
                                                  caccaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAg                                                                                          >  7:146002/1‑149 (MQ=255)
                                                    ccaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGc                                                                                        <  3:167297/149‑1 (MQ=255)
                                                     caccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCg                                                                                       <  7:94875/149‑1 (MQ=255)
                                                      accaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCgg                                                                                      <  1:79301/149‑1 (MQ=255)
                                                       ccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGTCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGt                                                                                     <  4:313732/149‑1 (MQ=255)
                                                         aaCGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTAATGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTa                                                                                   >  2:273227/1‑149 (MQ=255)
                                                          aCGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTAt                                                                                  <  4:53105/149‑1 (MQ=255)
                                                            gCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATct                                                                                >  1:220724/1‑149 (MQ=255)
                                                              ccACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgc                                                                              >  8:138056/1‑149 (MQ=255)
                                                               cacaTTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGGACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgct                                                                             <  3:69110/149‑1 (MQ=255)
                                                                acaTTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgctg                                                                            <  4:307490/149‑1 (MQ=255)
                                                                  aTTAACACCGGCGACGGAGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaa                                                                          <  7:19863/149‑1 (MQ=255)
                                                                       cacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaac                                                                     <  2:275427/149‑1 (MQ=255)
                                                                       cacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaa                                                                      >  6:175232/1‑148 (MQ=255)
                                                                         ccGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACAt                                                                   >  4:305780/1‑149 (MQ=255)
                                                                           ggCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATgg                                                                 <  7:138056/149‑1 (MQ=255)
                                                                           ggCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATgg                                                                 <  6:325385/149‑1 (MQ=255)
                                                                           ggCGACGGTGTCGGCATGGATATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGGGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATgg                                                                 <  7:138622/149‑1 (MQ=255)
                                                                                cGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAAc                                                            >  4:84506/1‑149 (MQ=255)
                                                                                   tgtCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGtt                                                         >  2:253153/1‑149 (MQ=255)
                                                                                     tCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGtttt                                                       <  6:58870/149‑1 (MQ=255)
                                                                                       ggCATGGCTATCCGTTCCGGCGTACCGTTGCAGGATATGGAAATGTGGCAGTTCCACACGACCTGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTTTCTGCTGAACAAACATGGCGAACGTTTTa                                                      <  5:291747/148‑1 (MQ=255)
                                                                                         cATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATg                                                    >  4:15686/1‑148 (MQ=255)
                                                                                          aTGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGa                                                  <  2:220724/149‑1 (MQ=255)
                                                                                           tGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAg                                                 >  8:227212/1‑149 (MQ=255)
                                                                                             gCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCATACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCg                                               <  3:51008/149‑1 (MQ=255)
                                                                                                aTCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATg                                                    <  3:253106/141‑1 (MQ=255)
                                                                                                aTCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATg                                                    >  4:253106/1‑141 (MQ=255)
                                                                                                    gTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGGACTGGTCACCGAAGGTTGCCGGGGTGCAGGCGGTTTTCTGCTGAACCAACATGGCGAACGGTTTATGGCGCGTTATgcg                                        >  2:187591/1‑149 (MQ=255)
                                                                                                             tACCGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGc                                >  2:95024/1‑148 (MQ=255)
                                                                                                                cGGTGCAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCaaa                            <  4:36717/149‑1 (MQ=255)
                                                                                                                     cAGGATATGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCt                       >  5:92284/1‑149 (MQ=255)
                                                                                                                           atGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCggg                 >  2:200743/1‑149 (MQ=255)
                                                                                                                            tGGAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCCGGCGGGc                >  7:333643/1‑149 (MQ=255)
                                                                                                                             ggAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAAc                                  >  4:92754/1‑130 (MQ=255)
                                                                                                                             ggAAATGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAAc                                  <  3:92754/130‑1 (MQ=255)
                                                                                                                               aaaTGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGCCCTGGCGGGCCGt             >  5:19425/1‑149 (MQ=255)
                                                                                                                               aaaTGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGt             >  1:16656/1‑149 (MQ=255)
                                                                                                                                aaTGTGGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGt             <  3:84506/148‑1 (MQ=255)
                                                                                                                                   gtgGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACg         >  8:104300/1‑149 (MQ=255)
                                                                                                                                    tgGCAGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGt        >  2:279015/1‑149 (MQ=255)
                                                                                                                                        aGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGTTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGtt    <  6:257977/149‑1 (MQ=255)
                                                                                                                                        aGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGtt    >  7:195538/1‑149 (MQ=255)
                                                                                                                                        aGTTCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGtt    >  1:20924/1‑149 (MQ=255)
                                                                                                                                          ttCCACACGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCTGTGACGTGGTTgc  <  5:175232/149‑1 (MQ=255)
                                                                                                                                             cacaCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGtt                                             >  6:107866/1‑103 (MQ=255)
                                                                                                                                             cacaCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGtt                                             <  5:107866/103‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGC  >  NC_000913/756489‑756775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: