Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,254,738 A→G 6.2% M150T (ATG→ACG)  rihB ← ribonucleoside hydrolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,254,7380AG 6.2% 200.2 / 3.1 67M150T (ATG→ACG) rihBribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base A (33/29);  new base G (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

GGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGA  >  NC_000913/2254591‑2254881
                                                                                                                                                   |                                                                                                                                               
ggggTGCAAACGGTCTGGGTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCAt                                                                                                                                                <  2:127830/149‑1 (MQ=255)
       aaaCGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGTCTGTACCGTAAGCGCCGCCCATTAGcac                                                                                                                                          >  7:179164/1‑148 (MQ=255)
                 gtttgtGAGTTCGAGGCCCTTCATCTCTAATGGTACGCCGGAGGTGTACTCTAGGGTTGGGGCTTCCGGGGCGGCAATGAGGTTGAACTCGGCAGATGGCGTTAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCAct                                                                                                                                 <  6:299221/147‑2 (MQ=255)
                  ttggtGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGAt                                                                                                                              >  3:14208/1‑149 (MQ=255)
                   tggtGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCa                                                                                                                                   >  6:53576/1‑143 (MQ=255)
                   tggtGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCa                                                                                                                                   <  5:53576/143‑1 (MQ=255)
                        agaTCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTggg                                                                                                                        >  6:151326/1‑149 (MQ=255)
                         gaTCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGc                                                                                                                       <  4:14208/149‑1 (MQ=255)
                           tCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAg                                                                                                                     >  7:147899/1‑149 (MQ=255)
                                gCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGATGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATcg                                                                                                                >  6:220936/1‑149 (MQ=255)
                                   catcatCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGCTCTCGGCAGATGGAGTGAAGTTGCCTGTCCCGTAAGCGCCGCCCTTTAGCACAATTTCAAGGATTTTGGGCAGGATCGCgg                                                                                                             >  2:105638/1‑149 (MQ=255)
                                     tcatcaCTAATGGACCGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGt                                                                                                           >  5:313710/1‑149 (MQ=255)
                                          aCTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCa                                                                                                       <  5:151326/148‑1 (MQ=255)
                                           cTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATa                                                                                                     >  2:10053/1‑149 (MQ=255)
                                            tAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATAc                                                                                                    >  3:53893/1‑149 (MQ=255)
                                              aTGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTCAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATAc                                                                                                    <  6:282952/147‑1 (MQ=255)
                                              aTGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACg                                                                                                   >  8:230603/1‑148 (MQ=255)
                                               tGGAACGCCGGAGGTGAACACTCCGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCa                                                                                                 >  5:20592/1‑149 (MQ=255)
                                                       cGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCAcc                                                                                         <  7:57104/149‑1 (MQ=255)
                                                        ggAGGTGAACACTACGCGGGCGGCTGCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGGGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGAGCGCGGGTTGCATACGCATTGCCACcg                                                                                        <  1:266144/149‑1 (MQ=255)
                                                            gTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGat                                                                                    <  5:288370/149‑1 (MQ=255)
                                                             tGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGTCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGata                                                                                   <  8:179164/149‑1 (MQ=255)
                                                                acacTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCTTTGCCACCGCGATAttt                                                                                <  7:230603/149‑1 (MQ=255)
                                                                      cgcgTGCGGCTTCCGGGTCGGCAAAGCTGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAg                                                                           <  5:15007/148‑1 (MQ=255)
                                                                      cgcgTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGaaa                                                                            <  8:147899/147‑1 (MQ=255)
                                                                        cgTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCgg                                                                        <  6:20592/149‑1 (MQ=255)
                                                                         gTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGa                                                                       <  4:53893/149‑1 (MQ=255)
                                                                                     ggTCGGCAAAGATGTTGCACGCGGCAGATGGCGTGAAGTTGCCTGGACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGGTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCAcc                                                           <  8:111070/149‑1 (MQ=255)
                                                                                        cGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTGGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGGTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCaga                                                        <  3:112990/149‑1 (MQ=255)
                                                                                         ggCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCaga                                                        <  1:10053/148‑1 (MQ=255)
                                                                                          gCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGt                                                      >  2:181904/1‑149 (MQ=255)
                                                                                          gCAAAGATGTGGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGCGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGt                                                      <  6:79275/149‑1 (MQ=255)
                                                                                            aaaGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGa                                                    >  1:179920/1‑149 (MQ=255)
                                                                                            aaaGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCAGTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGa                                                    >  6:260174/1‑149 (MQ=255)
                                                                                                 tGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGat                                                   >  7:279828/1‑145 (MQ=255)
                                                                                                 tGTTGAACTCGGCAGATGGCGGGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGat                                                   <  8:279828/145‑1 (MQ=255)
                                                                                                  gTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGACAGGATCGCGGGGTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGc                                              <  5:240738/149‑1 (MQ=255)
                                                                                                   ttGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGc                                              >  1:331579/1‑148 (MQ=255)
                                                                                                    tGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCa                                            >  3:327298/1‑149 (MQ=255)
                                                                                                       aCTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATAttt                                                                                <  5:68056/110‑1 (MQ=255)
                                                                                                       aCTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATAttt                                                                                >  6:68056/1‑110 (MQ=255)
                                                                                                         tCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGACAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCt                                       >  3:89955/1‑149 (MQ=255)
                                                                                                         tCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCt                                       >  3:125862/1‑149 (MQ=255)
                                                                                                         tCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCt                                       >  3:320107/1‑149 (MQ=255)
                                                                                                         tCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCt                                       >  4:212975/1‑149 (MQ=255)
                                                                                                         tCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGACCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCt                                       >  2:243938/1‑149 (MQ=255)
                                                                                                             cAGATGTCGTGAAGTTGCCTGTACCGTAAGCGCCTGCCATTAGCTCAATTTCACTGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAATGCGTACCAACCGGCACCAGAGTGATATCGCCATCGCTTGcc                                   <  3:264328/149‑1 (MQ=255)
                                                                                                                aTGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCAt                                 >  7:297937/1‑148 (MQ=255)
                                                                                                                aTGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATATCACGGATGTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATc                                >  4:98136/1‑149 (MQ=255)
                                                                                                                     gTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGCCCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAggg                            >  6:263943/1‑148 (MQ=255)
                                                                                                                             gCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGa                   >  2:90093/1‑149 (MQ=255)
                                                                                                                              ccTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGAGTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGat                  <  5:220936/149‑1 (MQ=255)
                                                                                                                                 gTACCGTAAGCGCCGCCCGTTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGc                                                                          >  1:122440/1‑90 (MQ=255)
                                                                                                                                 gTACCGTAAGCGCCGCCCGTTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGc                                                                          <  2:122440/90‑1 (MQ=255)
                                                                                                                                 gTACCGTAAGCGCCGCCCGTTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGc                                                                          >  3:299305/1‑90 (MQ=255)
                                                                                                                                 gTACCGTAAGCGCCGCCCGTTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGc                                                                          <  4:299305/90‑1 (MQ=255)
                                                                                                                                 gTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCa                               >  5:243603/1‑133 (MQ=255)
                                                                                                                                 gTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCa                               <  6:243603/133‑1 (MQ=255)
                                                                                                                                  tACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGatatat              <  2:179920/149‑1 (MQ=255)
                                                                                                                                     cGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCtt                                      <  1:244969/122‑1 (MQ=255)
                                                                                                                                     cGTAAGCGCCGCCCATTAGCACAATTTCACGGAATTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCtt                                      >  2:244969/1‑122 (MQ=255)
                                                                                                                                      gTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAAGGATATATTTCa          >  6:11694/1‑149 (MQ=255)
                                                                                                                                       tAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCAc         >  5:43273/1‑149 (MQ=255)
                                                                                                                                       tAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTAGCCAGCAGGGTATCAATCATATATTTCAc         >  8:173396/1‑149 (MQ=255)
                                                                                                                                           cgccgcCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCa     <  3:212975/149‑1 (MQ=255)
                                                                                                                                             ccgccCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCAt    >  7:166407/1‑148 (MQ=255)
                                                                                                                                              cgccCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGa  <  2:331579/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGCCTGTACCGTAAGCGCCGCCCATTAGCACAATTTCACGGATTTTGGGCAGGATCGCGGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGA  >  NC_000913/2254591‑2254881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑