Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 349,717 T→C 10.0% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913349,7170TC10.0% 149.1 / 11.5 72intergenic (+145/‑295)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (28/36);  new base C (4/3);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-04

AAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGG  >  NC_000913/349572‑349850
                                                                                                                                                 |                                                                                                                                     
aaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa                                                                                                                                     <  8:135057/148‑1 (MQ=255)
aaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGAc                                                                                                                                    <  2:254149/149‑1 (MQ=255)
 aaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACt                                                                                                                                   >  4:134633/1‑149 (MQ=255)
  aaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACtt                                                                                                                                  <  8:314681/149‑1 (MQ=255)
   aaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCAGCCGCCACCCGATGCATGATGCGCCGCTGACGTGACGTa                                                                                                                                 >  8:131636/1‑149 (MQ=18)
      gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                                                                              <  3:328648/149‑1 (MQ=255)
       cccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTGGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAg                                                                                                                             <  3:280597/149‑1 (MQ=255)
          gTTGATTGGATTCGCCAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGGAGGTCGGATACGACGGATGTCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGcc                                                                                                                          <  4:291512/149‑1 (MQ=14)
              aTTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                                                      >  3:214813/1‑149 (MQ=255)
               ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGACTGATGCGCAGCTGACGTGACTTATCAGGCATACgg                                                                                                                     >  4:282476/1‑149 (MQ=255)
                 gTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                    >  6:13706/1‑148 (MQ=255)
                 gTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                    <  5:13706/148‑1 (MQ=255)
                 gTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                    <  2:53671/148‑1 (MQ=255)
                 gTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                    <  2:315908/148‑1 (MQ=255)
                 gTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                    <  1:340720/148‑1 (MQ=255)
                  tATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt                                                                                                                  <  3:134633/149‑1 (MQ=255)
                  tATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt                                                                                                                  >  5:58165/1‑149 (MQ=255)
                   aTTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt                                                                                                                  <  8:282254/148‑1 (MQ=255)
                             cGATGCCGGATGCGTCGCTGACGCGACTTATCCGGCCTACGAGGTGCACGGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTg                                                                                                       <  7:131636/149‑1 (MQ=255)
                              gATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGt                                                                                                      <  6:58165/149‑1 (MQ=255)
                              gATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAGGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGt                                                                                                      <  6:278591/149‑1 (MQ=255)
                                    tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGGCTACGGGGTGCACTGATCTGTAGGTCg                                                                                                >  5:533/1‑149 (MQ=255)
                                        gCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACGGAAGTGTAGGTCGGAt                                                                                             >  1:68340/1‑148 (MQ=255)
                                                      tcTTATCAGGCCTACGAGGTGCACTGAAGTGTAGCTCGGATAAGACGGATGGCGTCGAATCCGACAACCGATGCCTGATGCTGCGCTGACGTGACTTATCAGGCCTACGTGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                              <  6:18334/148‑1 (MQ=14)
                                                        ttATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGGTGAACTGCCTTATCAGGCCTACGGGGTCCACTGAACTGTAGGTCGGATAAGACGAATatcaaag                                                                             >  1:230331/1‑142 (MQ=14)
                                                        ttATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGAGACTTATCAGGCCTACGGGGTGCCCTGAACTGTAGCTCTCATCAGACGAATAGCGTCg                                                                             >  3:199655/1‑148 (MQ=11)
                                                           tCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACAGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                                         <  4:72885/149‑1 (MQ=255)
                                                            cAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTACACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc                                                                        <  1:257556/149‑1 (MQ=255)
                                                                    cGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                 >  5:16024/1‑148 (MQ=39)
                                                                      aGGTGCACTTACCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt                                                              <  1:43154/149‑1 (MQ=255)
                                                                      aGGTGCACTGAACTGTAGGTCGGATAAGACGGAGGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCTTCCGACAACCGAt                                                              <  8:195856/149‑1 (MQ=255)
                                                                          gCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATTCCt                                                          >  1:235911/1‑149 (MQ=37)
                                                                              tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGa                                                                                                           <  4:243465/96‑1 (MQ=18)
                                                                              tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGa                                                                                                           >  3:243465/1‑96 (MQ=18)
                                                                                aaCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                    >  6:334537/1‑85 (MQ=255)
                                                                                aaCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                    <  5:334537/85‑1 (MQ=255)
                                                                                aaCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCAGATAACACGCATAGCGTCGCATCGCACAACCGAAGCCAGATgcg                                                    >  7:163376/1‑149 (MQ=11)
                                                                                       ggTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg                                                                                               <  6:199275/99‑1 (MQ=18)
                                                                                       ggTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg                                                                                               >  5:199275/1‑99 (MQ=18)
                                                                                       ggTCGGATAAGACAGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGGCAACCGCTGCCTGATGCGCCGCTGa                                             >  5:154889/1‑149 (MQ=255)
                                                                                           ggATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgc                                          >  2:106101/1‑148 (MQ=37)
                                                                                            gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                              <  7:148211/111‑1 (MQ=17)
                                                                                            gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                              >  8:148211/1‑111 (MQ=17)
                                                                                             aTAAGACGTATGGCGTCTCATGCGAGAACCGATGACTGATGCGCCGCTGACCTGACTTCTCAGGCCTTCGGTGTTCACTGAACTGTAGGTCGTATAAGACGCATATTGTCGAATTCGACAACCGATGCCTGATGCGTCACTGAcgc                                          <  8:167904/146‑1 (MQ=11)
                                                                                             aTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTTAACTGTAGTTCGGATAAGACGCATAGCGTCGCAACAGAAAACCGATGCCTGATGCGCCGCTGAcgctcc                                       >  6:114937/1‑146 (MQ=11)
                                                                                              tAAGACGGATGGCGTCGCATCCGCCAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGAGTCCCTTCGGGCCACCGAGGCCTCATGCGCCGCTGACGCGCCt                                      >  1:214311/1‑149 (MQ=11)
                                                                                              tAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                              >  5:108227/1‑93 (MQ=40)
                                                                                              tAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                              <  6:108227/93‑1 (MQ=40)
                                                                                                 gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc                                                            <  1:63155/124‑1 (MQ=38)
                                                                                                 gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc                                                            >  2:63155/1‑124 (MQ=38)
                                                                                                 gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAAGCCTGATGCGCCGCTGACGCGACTTAt                                   >  3:95130/1‑149 (MQ=37)
                                                                                                 gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCa                                                                                     >  5:33043/1‑99 (MQ=37)
                                                                                                 gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                              >  4:278272/1‑90 (MQ=18)
                                                                                                 caCGGATCCCGCCCCATCCGACAACCGATCCCTCATGCGCCGCTGACGTGACTTATCAGGCCTACGCGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt                                   <  6:533/148‑1 (MQ=11)
                                                                                                  tcGGATGGCGTCGCATCCGACAACCTATGCCTGATGCGGCGCTGACGTTACTTATCAGGCCTACGGGGTGCACTGAACTGTAGTTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATc                                  <  3:282476/148‑1 (MQ=255)
                                                                                                  aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAAGTGACATTTAAGGCCTAAGGTGTGCACTGAACTGTAGGTCGGATAAGACGCATAGATTCTCAACCGACAACCGATGCCAGCTGCGCAGCGGGCGGGCCTTAt                                   >  8:179004/1‑148 (MQ=11)
                                                                                                           gTCGCATCCGCCAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAAGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg                         <  4:95130/149‑1 (MQ=17)
                                                                                                           gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg                         <  6:16024/149‑1 (MQ=32)
                                                                                                              gCAGCCGACAAACGATGCCGGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACGGACCTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCGGATGCGCCGCTGACGCGACTTATCAGGCCTACgggg                      <  5:288277/149‑1 (MQ=11)
                                                                                                               cATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTTAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGt                     <  4:214813/149‑1 (MQ=17)
                                                                                                                    gACAACCGATGCCTTATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGTATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACt                <  2:94849/149‑1 (MQ=21)
                                                                                                                       aaCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGt                     >  3:159994/1‑141 (MQ=32)
                                                                                                                       aaCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGt                     <  4:159994/141‑1 (MQ=32)
                                                                                                                       aaCCGATGCCTGAGGCGCCGCTTCCGTTTCTTTTCAGGCCTAGGGTGTGCTCTGAACTGTAGGTCGGATAAGACGCATTGCGTCGCATCGGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa             <  8:163376/149‑1 (MQ=11)
                                                                                                                         ccGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACt           >  4:143958/1‑149 (MQ=34)
                                                                                                                          cGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGcc                                                           >  8:193946/1‑100 (MQ=33)
                                                                                                                                cTGTTGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTTGTTCGTATAAGACGCATAGGGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc    <  4:292403/149‑1 (MQ=11)
                                                                                                                                cTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGc                                                                                      >  3:238936/1‑67 (MQ=35)
                                                                                                                                cTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGc                                                                                      <  4:238936/67‑1 (MQ=35)
                                                                                                                                 tGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGAt                                                       >  2:351292/1‑97 (MQ=33)
                                                                                                                                   aTGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg  <  3:143958/148‑1 (MQ=34)
                                                                                                                                              aCGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                              >  3:78168/1‑45 (MQ=38)
                                                                                                                                                 |                                                                                                                                     
AAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGG  >  NC_000913/349572‑349850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: