Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,377,827 A→G 5.2% L478S (TTG→TCG)  menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,377,8270AG 5.2% 240.2 / 2.8 78L478S (TTG→TCG) menD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
Reads supporting (aligned to +/- strand):  ref base A (32/41);  new base G (2/2);  total (34/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

GTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGC  >  NC_000913/2377680‑2377973
                                                                                                                                                   |                                                                                                                                                  
gTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCaa                                                                                                                                                   >  6:52503/1‑149 (MQ=255)
 tGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAAc                                                                                                                                                  <  7:187806/149‑1 (MQ=255)
    gcCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGc                                                                                                                                               <  4:220346/149‑1 (MQ=255)
    gcCAGGCGTCGGAAAATGCAGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGATCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGATTTGCGGCGTTGGCAACAGc                                                                                                                                               <  8:297854/149‑1 (MQ=255)
           gTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGaaaa                                                                                                                                        <  7:287406/149‑1 (MQ=255)
            tCGGCAAATGCCGTTTCAACTTCCTCCCACTTTTGCGCACGAAGATATTTCATCACGAACATCCCGGAGGCGTTCTCAAAATAGACGTAATCCGCCATCAGAAATAAACGCTCACGCTCGCAATGCAGCGTATGCAAAAGCGAGaaaaa                                                                                                                                       >  8:96418/1‑148 (MQ=255)
              ggCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAAttt                                                                                                                                     >  8:176312/1‑149 (MQ=255)
               gCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAAttt                                                                                                                                     >  5:235185/1‑148 (MQ=255)
               gCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAAttt                                                                                                                                     <  6:235185/148‑1 (MQ=255)
                cAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGc                                                                                                                                   <  8:36501/149‑1 (MQ=255)
                cAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGc                                                                                                                                   >  5:80199/1‑149 (MQ=255)
                   aTGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGACCATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCTACAGCGAGAAAATTTGCccg                                                                                                                                <  2:248155/149‑1 (MQ=255)
                     gCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCccgcc                                                                                                                              >  4:49611/1‑149 (MQ=255)
                           tCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgt                                                                                                                         <  3:304886/148‑1 (MQ=255)
                            cAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCGCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgtt                                                                                                                        <  6:114575/148‑1 (MQ=255)
                              aGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgttgtt                                                                                                                     >  5:256597/1‑149 (MQ=255)
                               gTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgttgtt                                                                                                                     >  3:176272/1‑148 (MQ=255)
                                   cTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca                                                                                                                <  6:148541/149‑1 (MQ=255)
                                     gCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCGACAGCGAGAAAATTTGCCCGCCgttgttgt                                                                                                                      >  7:118705/1‑141 (MQ=255)
                                     gCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCGACAGCGAGAAAATTTGCCCGCCgttgttgt                                                                                                                      <  8:118705/141‑1 (MQ=255)
                                     gCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCGACAGCGAGAAAATTTGCCCGCCgttgttgt                                                                                                                      >  7:258801/1‑141 (MQ=255)
                                     gCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCGACAGCGAGAAAATTTGCCCGCCgttgttgt                                                                                                                      <  8:258801/141‑1 (MQ=255)
                                      ccAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca                                                                                                                >  8:74428/1‑146 (MQ=255)
                                      ccAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca                                                                                                                <  7:74428/146‑1 (MQ=255)
                                          ttttGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCaa                                                                                                                                                   <  3:264686/107‑1 (MQ=255)
                                          ttttGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCaa                                                                                                                                                   >  4:264686/1‑107 (MQ=255)
                                          ttttGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTaa                                                                                                         >  1:274929/1‑149 (MQ=255)
                                          ttttGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTaa                                                                                                         <  1:189616/149‑1 (MQ=255)
                                            ttGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTTACGCTCGCTTTCCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATa                                                                                                       >  2:22807/1‑149 (MQ=255)
                                            ttGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAAt                                                                                                        <  1:333050/148‑1 (MQ=255)
                                            ttGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAAt                                                                                                        <  7:214422/148‑1 (MQ=255)
                                                ggACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAg                                                                                                   >  2:219803/1‑149 (MQ=255)
                                                    gatgatATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgtt                                                                                                                        >  8:269948/1‑124 (MQ=255)
                                                    gatgatATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgtt                                                                                                                        <  7:269948/124‑1 (MQ=255)
                                                    gatgatATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAgcggc                                                                                               >  3:240464/1‑149 (MQ=255)
                                                     atgatATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAgcggcg                                                                                              <  3:49611/149‑1 (MQ=255)
                                                      tgatATTTCAGCTCGAACATCTCGGAGGCGGGGTCAAATTGGACGTTTTGCGGCATCATATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAgcggc                                                                                               <  2:86152/147‑1 (MQ=255)
                                                      tgatATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAgcggcg                                                                                              >  5:111383/1‑148 (MQ=255)
                                                           tttCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa                                                                                        <  3:322812/149‑1 (MQ=255)
                                                           tttCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTAGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAAAACCAGCGGCGCAGaaa                                                                                        >  6:306397/1‑149 (MQ=255)
                                                            ttCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTGCACCACAATTAATACCAGCGGCGCAGAAAc                                                                                       >  3:9566/1‑149 (MQ=255)
                                                                gCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGa                                                                                   <  5:52503/149‑1 (MQ=255)
                                                                 cTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc                                                                                  <  7:176312/149‑1 (MQ=255)
                                                                 cTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc                                                                                  <  8:311956/149‑1 (MQ=255)
                                                                 cTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc                                                                                  <  5:56783/149‑1 (MQ=255)
                                                                  tCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACg                                                                                 >  8:14059/1‑149 (MQ=255)
                                                                    gAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGTATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGc                                                                                >  5:140164/1‑148 (MQ=255)
                                                                     aaCATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCaa                                                                              <  7:133379/149‑1 (MQ=255)
                                                                         tCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc                                                                          >  4:187237/1‑149 (MQ=255)
                                                                         tCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc                                                                          >  3:48309/1‑149 (MQ=255)
                                                                                   gTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt                                                                <  6:111383/149‑1 (MQ=255)
                                                                                    tGCTCAATATGGTCGTTTTGCCGCAGCAGATCGAAACGCTCACGCGCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCTGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACCCCAGCGCGtg                                                               <  6:80199/149‑2 (MQ=255)
                                                                                    tGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                                                               <  7:14059/149‑1 (MQ=255)
                                                                                      cTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGATCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTGCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                                                             <  5:289239/149‑1 (MQ=255)
                                                                                         aaaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATCCCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgag                                                            <  5:269029/147‑1 (MQ=255)
                                                                                         aaaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGAGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                                                           <  7:211641/148‑1 (MQ=255)
                                                                                           aaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCg                                                        <  7:96418/149‑1 (MQ=255)
                                                                                             tGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                                      <  2:274929/149‑1 (MQ=255)
                                                                                               gACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                                      >  7:57788/1‑147 (MQ=255)
                                                                                               gACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGCCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                                                    <  3:306062/149‑1 (MQ=255)
                                                                                                   ttttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc                                                <  8:57788/149‑1 (MQ=255)
                                                                                                       gCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGGTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgag                                            >  3:269527/1‑149 (MQ=255)
                                                                                                                gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGcc                                    >  6:57876/1‑148 (MQ=255)
                                                                                                                     aaaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                              >  4:202976/1‑149 (MQ=255)
                                                                                                                         gctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGc                           >  7:343694/1‑148 (MQ=255)
                                                                                                                                   gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCgg                  <  1:22807/147‑1 (MQ=255)
                                                                                                                                   gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGtt                <  3:187237/149‑1 (MQ=255)
                                                                                                                                   gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGt                 <  4:176272/148‑1 (MQ=255)
                                                                                                                                   gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCAAAATTAATACCAGCTGCGCAGAAACCTGCCGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCCCAATCGCCAGCGTCGGtt                >  2:228255/1‑149 (MQ=255)
                                                                                                                                    ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGc                           <  4:72833/137‑1 (MQ=255)
                                                                                                                                    ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGc                           >  3:72833/1‑137 (MQ=255)
                                                                                                                                    ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGttt               <  4:48309/149‑1 (MQ=255)
                                                                                                                                     tttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTg              >  1:62395/1‑149 (MQ=255)
                                                                                                                                       tGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGc             <  6:140164/148‑1 (MQ=255)
                                                                                                                                           gcgTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCtt        <  1:219803/149‑1 (MQ=255)
                                                                                                                                            cgTTGGCAACCGCGAGAAAATTTGCCCGCCGTTGTTGTTCCCCACAATTAATACCCGCGGCGCAGAAACCTCACGCAATAAAGCCAGCGCGTTGAGCTCGAAAAGTGCGGAGAGATCACCCACAATCGCCAGCGTCGGTATCCCGCATg       >  7:209738/1‑149 (MQ=255)
                                                                                                                                                ggCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGccc    <  3:202976/148‑1 (MQ=255)
                                                                                                                                                ggCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCg   >  7:203778/1‑149 (MQ=255)
                                                                                                                                                 gCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCgg                  <  3:58814/133‑1 (MQ=255)
                                                                                                                                                 gCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCgg                  >  4:58814/1‑133 (MQ=255)
                                                                                                                                                 gCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGc  >  4:268678/1‑149 (MQ=255)
                                                                                                                                                   aaCAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg                                              >  8:143192/1‑103 (MQ=255)
                                                                                                                                                   aaCAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg                                              <  7:143192/103‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGC  >  NC_000913/2377680‑2377973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑