mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-1-BOP27-SUCB-END-3_S19_L001_R1_001.good.fq | 467,386 | 67,323,581 | 100.0% | 144.0 bases | 149 bases | 98.4% |
| errors | Plate-1-BOP27-SUCB-END-3_S19_L001_R2_001.good.fq | 467,386 | 67,229,940 | 100.0% | 143.8 bases | 149 bases | 97.8% |
| errors | Plate-1-BOP27-SUCB-END-3_S19_L002_R1_001.good.fq | 439,040 | 63,278,847 | 100.0% | 144.1 bases | 149 bases | 98.5% |
| errors | Plate-1-BOP27-SUCB-END-3_S19_L002_R2_001.good.fq | 439,040 | 63,208,302 | 100.0% | 144.0 bases | 149 bases | 97.4% |
| errors | Plate-1-BOP27-SUCB-END-3_S19_L003_R1_001.good.fq | 455,220 | 65,564,301 | 100.0% | 144.0 bases | 149 bases | 98.4% |
| errors | Plate-1-BOP27-SUCB-END-3_S19_L003_R2_001.good.fq | 455,220 | 65,474,157 | 100.0% | 143.8 bases | 149 bases | 97.7% |
| errors | Plate-1-BOP27-SUCB-END-3_S19_L004_R1_001.good.fq | 467,336 | 67,274,273 | 100.0% | 144.0 bases | 149 bases | 98.6% |
| errors | Plate-1-BOP27-SUCB-END-3_S19_L004_R2_001.good.fq | 467,336 | 67,199,229 | 100.0% | 143.8 bases | 149 bases | 97.5% |
| total | 3,657,964 | 526,552,630 | 100.0% | 143.9 bases | 149 bases | 98.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 110.6 | 6.4 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 19142 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 47 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.003 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.72118 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 01:51:32 12 Sep 2020 | 01:52:28 12 Sep 2020 | 56 seconds |
| Read alignment to reference genome | 01:52:28 12 Sep 2020 | 01:59:31 12 Sep 2020 | 7 minutes 3 seconds |
| Preprocessing alignments for candidate junction identification | 01:59:31 12 Sep 2020 | 02:00:28 12 Sep 2020 | 57 seconds |
| Preliminary analysis of coverage distribution | 02:00:28 12 Sep 2020 | 02:02:39 12 Sep 2020 | 2 minutes 11 seconds |
| Identifying junction candidates | 02:02:39 12 Sep 2020 | 02:02:51 12 Sep 2020 | 12 seconds |
| Re-alignment to junction candidates | 02:02:51 12 Sep 2020 | 02:04:55 12 Sep 2020 | 2 minutes 4 seconds |
| Resolving best read alignments | 02:04:55 12 Sep 2020 | 02:06:24 12 Sep 2020 | 1 minute 29 seconds |
| Creating BAM files | 02:06:24 12 Sep 2020 | 02:08:25 12 Sep 2020 | 2 minutes 1 second |
| Tabulating error counts | 02:08:25 12 Sep 2020 | 02:09:06 12 Sep 2020 | 41 seconds |
| Re-calibrating base error rates | 02:09:06 12 Sep 2020 | 02:09:08 12 Sep 2020 | 2 seconds |
| Examining read alignment evidence | 02:09:08 12 Sep 2020 | 02:17:27 12 Sep 2020 | 8 minutes 19 seconds |
| Polymorphism statistics | 02:17:27 12 Sep 2020 | 02:17:28 12 Sep 2020 | 1 second |
| Output | 02:17:28 12 Sep 2020 | 02:17:43 12 Sep 2020 | 15 seconds |
| Total | 26 minutes 11 seconds | ||
