Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,284,630	G→A	L85L (CTG→CTA)	yejM →	essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,284,630	0	G	A	100.0%	148.7 / NA	44	L85L (CTG→CTA)	yejM	essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

ACCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGC  >  NC_000913/2284485‑2284770                                                                                                                                                  |                                                                                                                                             aCCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCtaat                                                                                                                                            >  2:73074/1‑148 (MQ=255)   cTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAg                                                                                                                                         <  5:28214/149‑1 (MQ=255)      tttATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGtt                                                                                                                                      <  1:73074/149‑1 (MQ=255)          tCGCCGACTGGCCGTCAACGCTTGCTGGTCGCATTTATTCCTAGGTATGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGTTCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCtaat                                                                                                                                            <  1:135455/139‑1 (MQ=255)          tCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTg                                                                                                                                  >  1:87560/1‑149 (MQ=255)             ccGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTcc                                                                                                                               <  2:33720/149‑1 (MQ=255)                    gCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTc                                                                                                                        >  4:145353/1‑149 (MQ=255)                             gCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAAcgg                                                                                                               >  8:128943/1‑149 (MQ=255)                             gCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAAcgg                                                                                                               >  2:5285/1‑149 (MQ=255)                                 gCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAAcggcgg                                                                                                            <  1:5285/148‑1 (MQ=255)                                   tGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGaa                                                                                                         >  6:80535/1‑149 (MQ=255)                                    ggTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAAt                                                                                                        <  2:87560/149‑1 (MQ=255)                                          aTTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGc                                                                                                   >  1:49566/1‑148 (MQ=255)                                                     cGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATc                                                                                        <  5:80535/148‑1 (MQ=255)                                                      gTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCg                                                                                       >  3:15963/1‑148 (MQ=255)                                                           cATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGc                                                                                  >  7:120932/1‑148 (MQ=255)                                                             ttctCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGaa                                                                               <  8:86687/146‑1 (MQ=255)                                                             ttATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGaa                                                                               <  2:49566/149‑1 (MQ=255)                                                                  ggCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt                                                                          <  7:128943/149‑1 (MQ=255)                                                                    ccATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTa                                                                        <  4:24702/149‑1 (MQ=255)                                                                       tttCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGaa                                                                                                         >  1:91893/1‑113 (MQ=255)                                                                       tttCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGaa                                                                                                         <  2:91893/113‑1 (MQ=255)                                                                       tttCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTc                                                                     >  1:49328/1‑149 (MQ=255)                                                                                ccTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTtcca                                                              <  3:145353/147‑1 (MQ=255)                                                                                    gtgtTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCg                                                                                 <  4:26374/124‑1 (MQ=255)                                                                                    gtgtTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCg                                                                                 >  3:26374/1‑124 (MQ=255)                                                                                     tgtTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTtccatctccat                                                       <  2:110079/149‑1 (MQ=255)                                                                                       ttCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTccatctccatct                                                     <  2:49328/149‑1 (MQ=255)                                                                                              ccTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATcc                                               >  5:70309/1‑148 (MQ=255)                                                                                                  cTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATc                                           <  4:15963/148‑1 (MQ=255)                                                                                                     gCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTc                                        <  8:120932/148‑1 (MQ=255)                                                                                                      cTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTccatctccatct                                                     <  3:82276/134‑1 (MQ=255)                                                                                                      cTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTccatctccatct                                                     >  4:82276/1‑134 (MQ=255)                                                                                                         aTCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCGATTACTGATCGATTGAGAAGTCTTTACTAGTTTCCATCTCCATAATAATCCCATAGTCTGGc                                    >  8:77247/1‑148 (MQ=255)                                                                                                            gatttCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACt                                <  1:99529/146‑1 (MQ=255)                                                                                                                 cccGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGtt                            <  7:77247/148‑1 (MQ=255)                                                                                                                   cGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt                                                                          <  8:91415/100‑1 (MQ=255)                                                                                                                   cGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt                                                                          >  7:91415/1‑100 (MQ=255)                                                                                                                        aCCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTg                               >  8:135191/1‑138 (MQ=255)                                                                                                                        aCCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGAGCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTg                               <  7:135191/138‑1 (MQ=255)                                                                                                                          cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCaa                       <  7:82982/144‑1 (MQ=255)                                                                                                                          cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCaa                       >  8:82982/1‑144 (MQ=255)                                                                                                                          cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAAcc                     >  3:51059/1‑146 (MQ=255)                                                                                                                              aTCGTCGGCTCCCAGAGGCTAAAAAGGTTTTTGTCCGTAATTCTGGCAACGGCGGGAATTACGCTATTAAAGATCGAAATCGAAGTCTTTACACGTTTCCATATACATCTTAATACCATAGTCt                                       >  2:76592/1‑124 (MQ=255)                                                                                                                                       tCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGAt     >  2:157916/1‑149 (MQ=255)                                                                                                                                          cAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCATAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGgc  >  7:97084/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                             ACCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGC  >  NC_000913/2284485‑2284770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑