Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 762,928 1 bp→GG intergenic (+189/‑86) sucB → / → sucC dihydrolipoyltranssuccinase/succinyl‑CoA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913762,9271.G96.4% 287.6 / 3.4 83intergenic (+188/‑87)sucB/sucCdihydrolipoyltranssuccinase/succinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (45/35);  total (46/37)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01
*NC_000913762,9280CG100.0% 297.7 / NA 83intergenic (+189/‑86)sucB/sucCdihydrolipoyltranssuccinase/succinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (46/37);  total (46/37)

CCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGTGG  >  NC_000913/762785‑763077
                                                                                                                                                |||                                                                                                                                                      
ccTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAgggggt                                                                                                                                                   <  7:193690/149‑3 (MQ=255)
ccTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAgggggt                                                                                                                                                   >  7:60043/1‑147 (MQ=255)
   tctcCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAgggggtgac                                                                                                                                                >  4:235611/1‑144 (MQ=255)
    ctcCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAgggggtgac                                                                                                                                                <  3:235611/148‑6 (MQ=255)
        ggCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGAc                                                                                                                                             >  2:351351/1‑148 (MQ=255)
        ggCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGAc                                                                                                                                             >  2:346407/1‑148 (MQ=255)
         gCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACaa                                                                                                                                           >  2:357783/1‑149 (MQ=255)
         gCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACaa                                                                                                                                           >  2:267121/1‑149 (MQ=255)
            aTTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGc                                                                                                                                        >  2:156518/1‑149 (MQ=255)
             ttGAAGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGcc                                                                                                                                       <  6:165741/149‑1 (MQ=255)
               gAAGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGccc                                                                                                                                      <  8:25090/148‑1 (MQ=255)
                aaGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCg                                                                                                                                     >  3:199159/1‑148 (MQ=255)
                 aGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCgg                                                                                                                                    >  3:104801/1‑148 (MQ=255)
                   ccTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAg                                                                                                                                 >  3:7183/1‑149 (MQ=255)
                            aCGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGaa                                                                                                                        <  7:221210/149‑1 (MQ=255)
                             cGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGaaa                                                                                                                       <  1:267121/149‑1 (MQ=255)
                               cTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAata                                                                                                                     >  2:329819/1‑149 (MQ=255)
                                  aCGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTc                                                                                                                  <  1:351351/149‑1 (MQ=255)
                                  aCGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGTGTGACCGACAATGCCCGGAAGCGATACGAAATATTc                                                                                                                  <  1:346407/149‑1 (MQ=255)
                                   cgcgTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                                                                                 <  3:91679/149‑1 (MQ=255)
                                   cgcgTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                                                                                 <  8:60043/149‑1 (MQ=255)
                                            tCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGt                                                                                                        <  5:90617/149‑1 (MQ=255)
                                               ggCCTACGGGTCCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGttt                                                                                                      >  3:18416/1‑148 (MQ=255)
                                               ggCATACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTa                                                                                                     <  1:357783/149‑1 (MQ=255)
                                                gCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGTTGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTa                                                                                                     >  5:161343/1‑148 (MQ=255)
                                                gCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTTTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTaa                                                                                                    <  4:108053/149‑1 (MQ=255)
                                                 ccTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCGACGGTTTaa                                                                                                    >  6:334250/1‑148 (MQ=255)
                                                  cTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTaaaa                                                                                                  <  5:192485/149‑1 (MQ=255)
                                                  cTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTaaaa                                                                                                  <  1:156518/149‑1 (MQ=255)
                                                     cGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGa                                                                                                >  4:334085/1‑148 (MQ=255)
                                                      gggACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATa                                                                                              <  8:274967/149‑1 (MQ=255)
                                                        gACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATAtt                                                                                                                   <  3:156451/126‑1 (MQ=255)
                                                        gACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATAtt                                                                                                                   >  4:156451/1‑126 (MQ=255)
                                                          ccaccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGa                                                                                          <  6:213538/149‑1 (MQ=255)
                                                           caccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGAt                                                                                         <  1:272789/149‑1 (MQ=255)
                                                             ccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTa                                                                                       >  6:244574/1‑149 (MQ=255)
                                                              ctaTGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTTACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTAc                                                                                      <  6:350564/147‑1 (MQ=255)
                                                                aTGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTg                                                                                    <  1:9597/149‑1 (MQ=255)
                                                                  gTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTg                                                                                    >  3:163839/1‑147 (MQ=255)
                                                                   tAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGaa                                                                                  <  1:148708/148‑1 (MQ=255)
                                                                    ttgTCGGATGCGGCGCTTGCGCCTTATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAg                                                                                 >  8:41400/3‑148 (MQ=255)
                                                                     ggTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGa                                                                               >  1:92337/1‑149 (MQ=255)
                                                                      gTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAt                                                                              >  6:284853/1‑149 (MQ=255)
                                                                       tCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAATAGATAACGATTACTGAAGGATg                                                                             >  7:133741/1‑149 (MQ=255)
                                                                          gATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGa                                                                           >  1:206190/1‑148 (MQ=255)
                                                                          gATAACGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGt                                                                                                        <  1:100691/119‑1 (MQ=255)
                                                                           aTAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGa                                                                           >  2:107369/1‑147 (MQ=255)
                                                                            tAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAt                                                                              >  1:34897/1‑143 (MQ=255)
                                                                            tAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAt                                                                              <  2:34897/143‑1 (MQ=255)
                                                                              aGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGaa                                                                      <  5:171136/149‑1 (MQ=255)
                                                                               ggCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAac                                                                     <  4:104801/149‑1 (MQ=255)
                                                                                gcgcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTa                                                                                                     <  8:294482/116‑1 (MQ=255)
                                                                                gcgcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCGAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTa                                                                                                     >  7:294482/1‑116 (MQ=255)
                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATaa                                                                                             >  3:22694/1‑122 (MQ=255)
                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATaa                                                                                             <  4:22694/122‑1 (MQ=255)
                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAAcaca                                                                  >  8:179684/1‑149 (MQ=255)
                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAAcaca                                                                  >  2:73846/1‑149 (MQ=255)
                                                                                   cttgcgcCTTATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACAt                                                                 <  7:41400/146‑1 (MQ=255)
                                                                                      gcgcCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGaa                                                              <  4:7183/149‑1 (MQ=255)
                                                                                        gccgcATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACt                                                            >  4:304896/1‑149 (MQ=255)
                                                                                        gccgcATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACt                                                            >  4:266179/1‑149 (MQ=255)
                                                                                            cATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACa                                                        >  5:284064/1‑149 (MQ=255)
                                                                                             aTCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACAt                                                       <  2:220174/149‑1 (MQ=255)
                                                                                                  aCAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACAt                                                                 <  7:110581/134‑1 (MQ=255)
                                                                                                  aCAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACAt                                                                 >  8:110581/1‑134 (MQ=255)
                                                                                                     aGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCa                                              <  1:107369/149‑1 (MQ=255)
                                                                                                      gCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAg                                             >  7:10897/1‑149 (MQ=255)
                                                                                                          tGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCTATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTCCTGAAGGATTGACAGAACACATGAACTTTCATTAATCTAAGGCa                                          >  6:336462/1‑148 (MQ=255)
                                                                                                             cTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCaaaa                                       >  5:253765/1‑148 (MQ=255)
                                                                                                             cTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCAGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAaac                                      <  4:199159/149‑1 (MQ=255)
                                                                                                               gATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAaacaa                                    >  8:88350/1‑149 (MQ=255)
                                                                                                                 tgtgACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGCCCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATATCGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATACATGCaaatgat                                    >  8:7958/1‑144 (MQ=255)
                                                                                                                   tgACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACttt                                <  4:78267/149‑1 (MQ=255)
                                                                                                                   tgACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACttt                                <  7:88350/149‑1 (MQ=255)
                                                                                                                   tgACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACttt                                >  3:46709/1‑149 (MQ=255)
                                                                                                                    gACGTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACtttt                               >  7:19588/1‑149 (MQ=255)
                                                                                                                       gTTTAACGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGc                            >  2:54847/1‑149 (MQ=255)
                                                                                                                            aCGTGTCTTATCAGGCCTAGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTa                      >  8:22274/1‑149 (MQ=255)
                                                                                                                             cGTGTCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTa                      >  6:309760/1‑149 (MQ=255)
                                                                                                                              gtgtCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTAt                     <  3:334085/149‑1 (MQ=255)
                                                                                                                                gtCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATgg                   >  6:77967/1‑149 (MQ=255)
                                                                                                                                gtCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATgg                   >  2:165688/1‑149 (MQ=255)
                                                                                                                                 tCTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATgg                   <  6:284064/148‑1 (MQ=255)
                                                                                                                                  cTTATCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCt                 >  3:160720/1‑149 (MQ=255)
                                                                                                                                    tATCAGGCCTAGGGGGTGACCTACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCCGGCAACACAACTTTTTGCCCGCTATGGCTTa               >  4:290816/1‑149 (MQ=255)
                                                                                                                                      tCAGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTAc              >  2:4369/1‑148 (MQ=255)
                                                                                                                                        aGGCCTAGGGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCa            <  1:73846/148‑1 (MQ=255)
                                                                                                                                            cctagggggTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCAc        <  5:284853/143‑1 (MQ=255)
                                                                                                                                             ctagggggTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCAcc       <  3:304896/144‑1 (MQ=255)
                                                                                                                                                gggggTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGt    <  3:266179/147‑1 (MQ=255)
                                                                                                                                                gggggTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGt    >  3:17686/3‑149 (MQ=255)
                                                                                                                                                 ggggTGACCGACAATGCCCGGAGGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGTg   >  4:21103/2‑149 (MQ=255)
                                                                                                                                                  gggTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGTgg  <  6:161343/149‑1 (MQ=255)
                                                                                                                                                |||                                                                                                                                                      
CCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGTGG  >  NC_000913/762785‑763077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: