| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 94.9% | 109.6 / ‑0.1 | 39 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (21/16); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
ATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGT > NC_000913/3560317‑3560583 | aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAACGAACTGGAAGGCAGAGCCGCGCCGCCGTG‑AAGG‑cgcag > 1:96345/1‑139 (MQ=255) cgcggcggcgAGCCATTGGGCGTTCGCCGCGCCACTTTGCGGGCGAGGCGCTCCGTCTCGTCGGTCTGGGTGGCCGTGCGTGCGGGCCACGGCGTGGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGAt < 2:720608/139‑1 (MQ=255) cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCAGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCa > 1:702936/1‑143 (MQ=255) ggggcggtGGGGATTGGCGCCGCCGCTTGGCGGGCGATGCGCTCGTTGTCGTCGGTCTGGGTGGCCTTGCGATCGTGCCGCGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTg < 2:158921/136‑1 (MQ=255) tGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGT‑CGCAGGATCAGGTTTTGCTCCGcc > 1:201838/1‑143 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCGTCGGACTGGGTGGCCTGGTGGTCGTGCAACGGCGGGGTAACCGGACTGGAAGGCAGCGCCGCAGCGGCAGG‑ATGG‑CGCAGGAGCAGGTGTTGCACCGCCAggc > 2:412024/1‑141 (MQ=255) tttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTG‑ATGG‑CGCAGGATCAGGGTTTGCTCTGCCAGCTCa > 2:435099/1‑143 (MQ=255) ccTCGGCCATTTTCCGGGCTGTGCGCGCGTTTTGTTCGCTCTGGGTTGCCTTGCGATCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGTG‑CAGGCTCAGGTTTTGCTCCGCCAGCTCATTg < 2:677537/142‑1 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg > 1:438592/1‑143 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg > 2:686400/1‑143 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCCTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg < 1:166584/143‑1 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGt > 1:167039/1‑143 (MQ=255) gCCACTTTGCGGGCGATGCGATCTTTTTCTTCAGCCTAAATTTCCTTGCGATCGTGCCACGGCGTGTTAACCTAACTGGTATTCAGCGCCTCATCGCCATGAATAG‑G‑CAGGATCAGGTATTGCTCCCGCAGCTCATTGAGGtg > 2:15255/1‑142 (MQ=255) cACTTTGCGGGCGATGCGCTCTTTTTCTTCGGACTGGGTGGCCTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCATG‑CTGG‑CGCCGGATCAGGTGTGGCTCCGCCAGCGCATTGAGGgcgc > 1:233750/1‑143 (MQ=255) aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGACAGCTCATTCAGGCcgcg > 2:325855/1‑143 (MQ=255) cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg < 1:322715/143‑1 (MQ=255) gcgggcggTGGGCCTGTTTTCTTCGGTCTGGGTGGCCTTGCGTTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑AGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa < 1:132274/143‑1 (MQ=255) gCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt > 1:474867/1‑143 (MQ=255) cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg < 1:777905/143‑1 (MQ=255) cgcTCTTTCGCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg < 1:85018/143‑1 (MQ=255) tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt > 2:551704/1‑143 (MQ=255) ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGTCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAATCGTCTGCGGGCtc > 2:33766/1‑142 (MQ=255) gctgcGGTGTGGGTGGCGTTGCGGTCGTGCGACGGCGTGTGAACCGAACGGGAAGGCAGCGCCGCACCGCGATG‑AGGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga < 2:561759/139‑1 (MQ=255) ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgagccat > 2:422842/1‑139 (MQ=255) ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt > 2:480889/1‑143 (MQ=255) ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc < 2:474867/143‑1 (MQ=255) cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc < 2:263005/143‑1 (MQ=255) gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑CTGG‑CGCAGGATCAGGTCTTGCCCCGCCAGCTCACTGAGGTCGCGGCGACTCGTCTGCGGGCTGAGGGAGAAAAGCTCCACCCGCTCTCCg > 1:169462/1‑143 (MQ=255) aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg < 1:811015/143‑1 (MQ=255) cacggAAGTGGAGGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATGGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:303318/139‑1 (MQ=255) aCCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg > 1:546603/1‑143 (MQ=255) aCCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACATAACCctgctg > 2:190955/1‑143 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTCTAAcc > 2:729498/1‑143 (MQ=255) gggAGGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc < 2:591059/142‑1 (MQ=255) gAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCTGCTTAACCac > 1:710220/1‑142 (MQ=255) gggCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGTTCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 2:559950/142‑1 (MQ=255) catggccgcACCGCCATG‑ATGG‑CGCAGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa < 2:606906/139‑1 (MQ=255) cgcgcACCGCCATG‑GTGG‑CGCATGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCAGCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat < 2:591359/141‑1 (MQ=255) gcACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGATCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACATAACCCTGCTGTTAAACCAGTCCGATACTAcc > 2:528341/1‑143 (MQ=255) aCCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTCTAACCAGTTCGATAATACAGt > 1:596015/1‑143 (MQ=255) | ATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGT > NC_000913/3560317‑3560583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |