Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1050545NA (NA)50 (1.200) 43/282 0.0 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 1293032 =0 (0.000)52 (1.250) 41/282 0.1 100% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)noncoding (1/768 nt) IS1 repeat region

AGCCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293173‑1293032
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGC  <  NC_000913/1979270‑1979129
                                                                                                                                                                                                                                                                                             
cGCCGACGATAATCTCCCCAGATAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAG                                                                                                                                                <  2:484835/142‑1
  CCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGT                                                                                                                                              >  1:613831/1‑143
  CCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCCGCTTTTCTGGCTAAGTTTATGAAAAGATCTTGCTTGGCCTCACAAGGT                                                                                                                                              >  2:220706/1‑143
    TACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGA                                                                                                                                            >  1:690416/1‑143
       GATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGC                                                                                                                                         >  2:155580/1‑143
                  CATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATCTTTATTGGCGGCACAAGGTGATGCTGCCAACTTAC                                                                                                                              >  2:591634/1‑143
                       AATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATT                                                                                                                         <  1:707188/143‑1
                           ttACATGAATCAGTAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGTGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGT                                                                                                                     <  2:52916/141‑1
                           tgcCATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGT                                                                                                                     <  1:64514/140‑1
                             ACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGT                                                                                                                   >  1:52003/1‑143
                               ATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGAGTAT                                                                                                                 >  2:89463/1‑143
                                 GAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑A                                                                                                              >  2:542939/1‑143
                                   ATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATG                                                                                                            <  1:463180/143‑1
                                        GAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTT                                                                                                       >  1:674831/1‑143
                                        GAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGGGTT                                                                                                       >  2:434340/1‑143
                                           ggTTTAGCCTCACTTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTT                                                                                                    <  2:403971/141‑1
                                             gTTAACCGCGCGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGA                                                                                                  <  2:197544/142‑1
                                             TTTAACCTCCCTTGCTGCGAAAGCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGA                                                                                                  <  2:611408/143‑1
                                              TTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAG                                                                                                 <  2:690416/143‑1
                                                AACCTCACGTGCTGCGAAATCATCGGGGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGT                                                                                               <  2:5907/143‑1
                                                  CCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGC                                                                                             <  1:415272/143‑1
                                                    TCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTC                                                                                           >  1:235831/1‑143
                                                     CACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCC                                                                                          >  1:601745/1‑143
                                                      ACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAGTTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTGGTGTAGG‑ATGGTGTATTTGACGTGATCga                                                                                         >  2:601558/1‑141
                                                              CGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTC                                                                                 >  1:615023/1‑143
                                                               GAAACCATCGGTGTAAATATGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGTTGCACAAGGTGATGCTGCCAACTTACTGATGTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCT                                                                                <  1:707824/143‑1
                                                                    CATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTC                                                                           <  1:335088/143‑1
                                                                     ATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCT                                                                          <  2:486380/143‑1
                                                                      TCGGTGTAAATAGGGCTATATGCCGGGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTA                                                                         <  2:637654/143‑1
                                                                       tGGTGTATATGGGGCTATCTTCCGCGTCTTTTCTGGCTAATTTTGTGAAAGGCTATTTATTGGCGGCACAAGGTGTTTCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTGTGAGGTGCTCCAGTGGCTTCTGTTTCTAT                                                                        <  2:380993/142‑1
                                                                          TGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCACTGGCTTCTGTTTCTATCAG                                                                     <  1:656315/143‑1
                                                                           GTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGATGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGCGCTCCAGTGGCTTCTGTTTCTATCAGC                                                                    >  2:646052/1‑143
                                                                              aagAGGGTTATATGCCGCGTCTTTTCTGGTTAATTATATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGT                                                                 <  2:720930/140‑1
                                                                                   GGCTGTATGCCGCGTCTTTTCTGGCTAATTTGATGAAGAGATATTTATTGGCGGGGCGTGGGGATGCTGCCAACTTACTGATTTAGTGTATG‑CTGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTC                                                            <  2:265429/143‑1
                                                                                   GGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTC                                                            <  1:716340/143‑1
                                                                                          TGCCCCGTCTTTTCTGGCTCATTTTATGAAAAGATCTTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCA                                                     <  2:19099/143‑1
                                                                                           GCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAG                                                    <  1:319820/143‑1
                                                                                                      TCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGGCCCTCCTGTTCAGCTACTGACGGG                                         <  1:577216/143‑1
                                                                                                         gcgtgATTTTATGAAAAGATAGTTATTGGCGGCACAAGGTGATGCTGCCAACTTGCTGATTTAGTGTAGG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTGTGTGTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTG                                      <  2:55686/138‑1
                                                                                                            TAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGTTAATTATGTGGTGGTG                                   >  1:205571/1‑143
                                                                                                            TAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGGCGGGGTGGTG                                   >  1:205570/1‑143
                                                                                                             AATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAGGGGTTGGTGC                                  >  2:353251/1‑143
                                                                                                               TTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGATGGGGTGGTGCGT                                >  1:298426/1‑143
                                                                                                                 TTATGAAAAGATATTTATTGGCGGCACATGGTGATGCTGCCATCTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTTCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAA                              <  2:149216/143‑1
                                                                                                                 TTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ACGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTGCAGCTACTGACGGGGTGGTGCGTAA                              <  2:613831/143‑1
                                                                                                                   ATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACG                            <  2:235831/143‑1
                                                                                                                         agagagTTATGGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAA                      <  1:259763/137‑1
                                                                                                                         AGATATTTCTTGGCTGCTCTATGTGTTGCTGCTATCTTATTGATTTAGTGTATT‑ATGGTGTTTTTGAGTTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCTCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAA                      <  2:594611/143‑1
                                                                                                                               TTATTGGCGGCACAAGGTGATGATGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCG                >  1:436293/1‑143
                                                                                                                                  TTGGCGGCACAAGGTGATGCGGCCAACTTACTGATTTCGT‑TCTGTAGGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCG             <  1:241442/143‑1
                                                                                                                                             AGGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGC  <  2:317087/143‑1
                                                                                                                                             AGGTGATGCTGCCAACTTACTGAATTAGGGTTAG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGAGAGTCACGGCACAAGCACCGCGGGACACCCGCGC  >  2:462610/1‑143
                                                                                                                                                                                                                                                                                             
AGCCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293173‑1293032
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATG‑ATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGC  <  NC_000913/1979270‑1979129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.