Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	523,618	A→G	13.1%	S120G (AGC→GGC)	rhsD →	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	523,618	0	A	G	13.1%	202.7 / 2.3	86	S120G (AGC→GGC)	rhsD	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (21/50);  new base G (5/9);  total (27/59)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCT  >  NC_000913/523345‑523886                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                gnggggccacccGGCGGGGGGGGCGCGGTGGGTGCGGGCGGGATGACTTCGGGGAACCCGGTAAATCCGCTGGTAGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGGTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGa                                                                                                                                                                                                                                                                                 <  2:1146548/264‑1 (MQ=25)   cgcgcCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑Gc                                                                                                                                                                                                                                                                              >  1:464748/1‑274 (MQ=255)   cgcgcCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCa                                                                                                                                                                                                                                                                             >  1:1477338/1‑275 (MQ=255)                gggtggtgCGCGGGGTGTCCGGTGGGGATGACTTCGGGCGAACCGGGAAGTCCGCTGCTGGGGTCGAAGTTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCTGCCGGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGc                                                                                                                                                                                                                                                                 <  2:1036304/269‑1 (MQ=37)                  ggcgtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATCTCCGCTTACAGCTACGTGATGCCGGCCTGATACTCAACCACAACGGCGGGCGGG‑GCAATCCCTTTGAGcccg                                                                                                                                                                                                                                                              >  2:447627/1‑273 (MQ=255)                      tgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGACGGCGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCAGCTGCCGTTCCTTTTCTCCCGCACCTACAGCAGCTACCGGACGACGACGCCTGCCCCGGTGGGCGGTTTCGGCCCCGGCTGGAAAACGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACCCAACGACAACGGCGGGCGGG‑GCATTCTCTTTGCGCCcccgct                                                                                                                                                                                                                                                          >  1:138589/1‑275 (MQ=255)                      tgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTCAGCCgctgcc                                                                                                                                                                                                                                                          >  1:1101226/1‑274 (MQ=255)                        cTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGCCGGGCGGA‑GACTTCACTTTGAGCCgctgct                                                                                                                                                                                                                                                          >  1:778388/1‑273 (MQ=255)                             tgtgtCCGGGCGGGATGACTTCGGGCACCCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTCCAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATCCTCAACGACAACGGCGGGGGGAGGCATCCCCTTT‑CGCCCCTGCTCCCggg                                                                                                                                                                                                                                                    >  2:214260/1‑274 (MQ=25)                                 tCCGGGCGGCATGACGGCGGGCAACCCGGGAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCAAAGCGGACCTT‑GCGCTGCCCGTCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCggggg                                                                                                                                                                                                                                                  <  1:1345478/272‑1 (MQ=255)                                  ccGGGCGGGGTGACTTCGGCCAACCGGGTAAATCCCCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAgg                                                                                                                                                                                                                                               <  2:464747/274‑1 (MQ=255)                                       cgggATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGGTGCCGGGGGAGGCGgtg                                                                                                                                                                                                                                          <  1:389271/274‑1 (MQ=255)                                        gggATGCCTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGTTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTa                                                                                                                                                                                                                                        <  1:1391068/275‑1 (MQ=255)                                         ggATGGCGTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGCCGGACCTT‑GCCCGGCCCGGCCCCCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGGCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCGGACATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTa                                                                                                                                                                                                                                        <  2:474534/274‑1 (MQ=255)                                           aTGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGTCGGGGGGGGCGGTGTaacg                                                                                                                                                                                                                                     >  1:38564/1‑272 (MQ=255)                                             gcctgcGGGCAACCCGGTACATCCGCTGCTGGGGGCGAAGGTGCAGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTCCAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGATGTACAGc                                                                                                                                                                                                                                    <  1:847060/269‑1 (MQ=255)                                                ttCGGGCAACCCGGTAGATCCCCGGCTGGGGGCGAAGGTGCGGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCa                                                                                                                                                                                                                                <  2:38564/275‑1 (MQ=255)                                                ttCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCCACGTGATGACGGACTGATACTCAACAACAACGGCGGGCGGG‑GCCATACCTTTGAGCCCCCGCCTCCGGGGGGGGCGGTGGACAGCCGcc                                                                                                                                                                                                                                >  2:35725/1‑274 (MQ=37)                                                gtCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCa                                                                                                                                                                                                                                <  1:664854/274‑1 (MQ=255)                                                 gcGGGCAACCCGGTAAATCCGCTGCTGGGGACGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCa                                                                                                                                                                                                                                <  1:992927/273‑1 (MQ=255)                                                              tAAAGCCGCTGCTGGGGCCGAAGGGGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTGCATTCTCTCCCGCACCGACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACGGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGTGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTgg                                                                                                                                                                                                                  <  1:657974/275‑1 (MQ=255)                                                               aaaTCCGCTGCTGGGGGAGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTACACTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGc                                                                                                                                                                                                                 <  1:32111/275‑1 (MQ=255)                                                                      ctgctgGGGGCGAAGGTGCTGCCCGGCGAGGCGGACCTT‑GCGCTGCCCGGCCCGCGGCCGTTCATTCTCTCCCGCACTTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTTGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑g                                                                                                                                                                                                         <  1:1341369/275‑1 (MQ=255)                                                                       tgctgGGGGCGAAGGTGCTGCCCGGCGAGCCGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  1:704732/275‑1 (MQ=255)                                                                                       gctgcCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGgcagca                                                                                                                                                                                         <  1:107149/274‑1 (MQ=255)                                                                                             cGGCGAGGCGGACCTT‑TCGCTGCCCGGCCCGCTGCCGTTCCTTCTCTCCCTCACCTACAGCAGCTACCGGACGAAGACGCCTGGACCGGTGGGCGTTTTCGGCCCCGGCTGGAAGGCGCCTTCTGATATCCGCTTACAGCTACGTTATGACGGACTGATACTCAACGACAACGGCGGTCGGG‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCg                                                                                                                                                                                   <  2:665524/274‑1 (MQ=255)                                                                                             cGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCTTTCACTTTGAGCCGCTGCTGCCCGGGGGGGGGGGGTCCGGCCGCAGTGGCTCAATGTGGGTGGTTC‑GCCGTGGTGAGGACGCACAACCgg                                                                                                                                                                                  >  2:135319/1‑275 (MQ=37)                                                                                             cGGCGAGACGGACCTT‑GCGCTGCCCGACCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCg                                                                                                                                                                                   <  1:1244610/274‑1 (MQ=255)                                                                                               gCGAGACGGCCCTTGGCGGTGCCTGG‑CCGCCGCCGTTCATTGTCTCCCGCGCCTACGGCAGGTACGGGACGAAGACGCCTGAGCCGGTGGGCGTTTTCGGCCCCGGCTGGATAGCACCTTCTGAGATCCGCGTACGGCTACGGGATGACTGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGGGCCGCTGCGGCCGGGGTAGGCGGTGTACAGCCGCAGGGAGTCAATGTGGCTGGTGC‑GCGGAGGTAAGGCAGCACAGCCGGa                                                                                                                                                                                 <  2:138588/274‑1 (MQ=255)                                                                                                            t‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTgg                                                                                                                                                                   <  1:135320/275‑1 (MQ=255)                                                                                                                gcTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGgcg                                                                                                                                                                 <  1:35725/274‑1 (MQ=255)                                                                                                                 cTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGa                                                                                                                                                                                 <  1:748597/257‑1 (MQ=255)                                                                                                                  tccccggaccgCTGCCGTTCAGTCTCTCCCGCACCTACGGTAGCTACCGGACGAAGACGCCTGGACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGcgcg                                                                                                                                                               <  1:886207/272‑1 (MQ=255)                                                                                                                    cccggcccgCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCCTTCCCTCTTAGCCGCCCCTGCCCGGGGGGGCGGGGTGCAGCCGCAGCGAGTCAAAGTGGCGGCGGC‑GCGGGGGGAAGGGGCAACACCCGGACGGCCATCCgacgggggggc                                                                                                                                                             >  2:1051700/1‑264 (MQ=21)                                                                                                                    cccggcccgCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCgg                                                                                                                                                              <  1:1319133/273‑1 (MQ=255)                                                                                                                         cccgCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCTTTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  1:1103478/226‑1 (MQ=255)                                                                                                                              tGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCACTCACTTTGTGCCCCCGCCGCCCGGGGAGGGGGGGTTACGCCCCCGTGGGGCAAAGTGGGCCGTGC‑GCGGGGGGAAGGCGGCACCGCGCGGCCGCCATCAACCCGCGCGGACGTggggggg                                                                                                                                                   >  2:484449/1‑273 (MQ=21)                                                                                                                              tGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCa                                                                                                                                                                                                                                >  1:497505/1‑198 (MQ=255)                                                                                                                                  gTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGa                                                                                                                                                                                                                            <  2:497504/198‑1 (MQ=255)                                                                                                                                        tctctcCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGCGGGGGGCGCTGCCGCCgg                                                                                                                                        <  1:447628/275‑1 (MQ=255)                                                                                                                                            tcCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCGACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGatat                                                                                                                                    <  1:1051701/275‑1 (MQ=255)                                                                                                                                            tcCCGCACCGACGGCAACTACCGTGCGACAAAGCGTGAACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCAGTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGatat                                                                                                                                    <  2:865993/275‑1 (MQ=255)                                                                                                                                               cGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCggggg                                                                                                                                                                                                                                                  <  1:1018516/163‑1 (MQ=255)                                                                                                                                                 cACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCAATTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGGGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGGCTAGCCGGACGGGCACACGCTGGCCGGGCTGTGGGGGGCGCTGCCGCCGGATAAACGGt                                                                                                                               >  1:1585351/1‑275 (MQ=255)                                                                                                                                                  aCCGACGGCAGCTGCCGGACGAAGACGCCTGCACCGGTGGGCGTTGTCGGCCCCGGCGCGAAGGCGCCTTCTGATATCCGCTTACAGCTAGGTGATGACGGACTGACACTCAACGACAACGGCGGGCAGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCGGTGAGTGAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGCGCGCTGCCGCCGGATATCCGGtt                                                                                                                              <  2:87803/275‑1 (MQ=255)                                                                                                                                                      aCAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGTA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  1:535229/197‑1 (MQ=255)                                                                                                                                                         gcagcTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCg                                                                                                                       <  1:484450/275‑1 (MQ=255)                                                                                                                                                         gcagcTACCGGACGAAAACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCg                                                                                                                       <  1:48149/275‑1 (MQ=255)                                                                                                                                                            gcTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCATTCACTTTGAGCCGCTGCTACCGGGGGAGGCGGGGTGCAGCCGCCGCGAGGCAATGTGGCTGGCGC‑GCGGGGGTAAGGCAGCACAGCCGGACGGCCCTACGCTGGCGCGCGGGGGGGGGGCGCTGGCCCCCGGCACCCGGTTCAGCCCGcg                                                                                                                     >  2:788095/1‑273 (MQ=255)                                                                                                                                                             cTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCTCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGGGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATAAGCTGGCGCGGGTGTGGGGGGCGCTGCCGCCGGATATACGGGTAAGGCCGcca                                                                                                                    >  1:1209843/1‑272 (MQ=255)                                                                                                                                                              tACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATTCGCCGGCGCGGCCGTGGGGGGCGCGGCCGCCGGATATTCGGTTAAGCCCCCaccc                                                                                                                  >  1:76051/1‑272 (MQ=255)                                                                                                                                                              tACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCCCTGCTGCCGGGGGGGGCGGGGTTCAGCCGCAGTGAGTCAATTGGGGCGGTGCGGGGGGGGTAGGGCGCCCCGGCCGGCGGGCCAAACCCGGGCCGGGCGGGGGGGGGGGTGGCCG‑CGGGGATCCGGttt                                                                                                                             >  2:1320397/1‑263 (MQ=255)                                                                                                                                                                  ggACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGcc                                                                                                                         <  1:1320398/264‑1 (MQ=255)                                                                                                                                                                   ggagAAGACGCCTGCACCGGTGGGCTTTTTCGGCCCCGGCGGGAAAGCGCCTTCGGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGGCACCGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTAcc                                                                                                             <  2:25832/272‑1 (MQ=255)                                                                                                                                                                     cGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTg                                                                                                           <  2:372559/275‑1 (MQ=255)                                                                                                                                                                            gCCTGAACCGGGGGGCGGTTGCGGCCCCGGCTGGGAAGGGCCTTCGGATATCCGCTTACAGGTACGTGGTGACGGACTGATGCTCAGCGACAACGGCGGGCGGA‑GCCTTCACTTTGGGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGGGAGGCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGAc                                                                                                      <  2:547244/273‑1 (MQ=255)                                                                                                                                                                              cTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGC‑GCATTCACTTTGACCCGCTGCTGCCGGGGGAGGCGGGGTACAGCCCACGTGAGTCAATGTGGCTGGGGC‑GCGGGGGTAAGGAAGCCCAGACGGGCAGCCATACGCCGGCGCGGGCGGGGGGGGCGCGGCCGCCCGGTTCCCGGTTTAGGCCGCACATTTTCCTGGCGACCaca                                                                                                  >  2:1436583/1‑273 (MQ=255)                                                                                                                                                                              cTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATCCACTTTGAGCCGCTGCTGCCg                                                                                                                                                                                                                                                      >  1:1552256/1‑128 (MQ=255)                                                                                                                                                                              cTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCCTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCGCTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGGCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGGGGGGGGCGCGGCCGCCGGCTGTCCGGTTAAGCCCGCCTGTTTACCTGGCGACCCAc                                                                                                  >  1:215498/1‑275 (MQ=255)                                                                                                                                                                                  aCCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATCCACTTTGAGCCGCTGCTGCCggggg                                                                                                                                                                                                                                                  <  2:1552255/128‑1 (MQ=255)                                                                                                                                                                                    cGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGGGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCCGTGGGGGGCGCTGCCGCCGGGTATCCGGTTAAGCCCGCATCTGTACCTGGGGCCCAACCGCGca                                                                                            >  1:1180414/1‑275 (MQ=255)                                                                                                                                                                                     ggTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  1:349960/166‑1 (MQ=255)                                                                                                                                                                                          gCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAgggg                                                                                      <  1:398590/275‑1 (MQ=255)                                                                                                                                                                                            ggttgcGGCCCCGGCTGGAAAGCGCCGTCTGATATCCGCGTACAGCTACGTGATGACGGACTGTTTCTCAACGACAACGGCGGGCGGG‑GCATTCACTTTGAGCCGCTGGTGCCGGGGGAGGCGGTGGACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGcc                                                                                    <  2:243633/270‑1 (MQ=255)                                                                                                                                                                                            gTTGTCGGCCCCGGCCGGAAAGGGCCTGCTAATTGCCGCCTACAGCCACGGGATTACGGACTGCAACTCACCGAGAACGGCGGTGTGG‑GCCTTCACTTTGGGCCGCTGGTGCCGGGGGAGGCGGTGTACAGCCGCCGGGAGGCAATGTGGCTGGTGC‑GCGGTGGTAAGGCGGCACAGCCGGAAGGCGATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCTGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGcc                                                                                    <  2:1101225/275‑1 (MQ=255)                                                                                                                                                                                              tttCGGCCCCGGCTTGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGGCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTaa                                                                                                                            >  1:756846/1‑233 (MQ=255)                                                                                                                                                                                                 cGGGCCCGCCTGGAAGGCGCCTTGTGGTAGCCGCTTACAGCTACGTGAGGACGGACGGGTAGCAAATGACAACGGCGGGGGGA‑GCATTCGCTGTGAGCGGCTGGTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCGGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCgtggt                                                                               <  2:13718/275‑1 (MQ=255)                                                                                                                                                                                                  ggCCCCGGCTTGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGccc                                                                                                                        <  2:756845/233‑1 (MQ=255)                                                                                                                                                                                                      ccGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑a                                                                         <  1:322757/275‑1 (MQ=255)                                                                                                                                                                                                      ccGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑a                                                                         <  1:214261/275‑1 (MQ=255)                                                                                                                                                                                                      ccGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑a                                                                         <  1:1321988/275‑1 (MQ=255)                                                                                                                                                                                                        ggCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAAtg                                                                                                                                                                                                                     >  1:91688/1‑135 (MQ=255)                                                                                                                                                                                                            ggAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGc                                                                                                                                                                                                                 <  2:91687/135‑1 (MQ=255)                                                                                                                                                                                                                   gcCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGGGAGTCAATGTGGCTGGTGG‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCGGTGGGGGGCGCTGCCGCCAGATATCCGGTTAAGCCGGCATCTTTACCTGGCGCCCAACAGCGCACAGGGGCCGTGGGGGATCACGGGGGGGGTcg                                                             >  1:1582632/1‑274 (MQ=255)                                                                                                                                                                                                                               ccGCGTACAGCTACGGGATGCGTGGCTGATCCTCAACGACAACGGCGGGCGGG‑GCATTCACTTTGGGCGGCTGCTGCCGGGGGGTGCGGTGTACAGGCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGcgggtgcc                                                  <  2:778387/273‑1 (MQ=255)                                                                                                                                                                                                                                    tACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGcaca                                                                                          <  1:1306873/229‑1 (MQ=255)                                                                                                                                                                                                                                      cAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCgtggt                                                                               <  1:1124806/238‑1 (MQ=255)                                                                                                                                                                                                                                                 tgaCGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAg                                                                                                                           >  1:1002919/1‑183 (MQ=255)                                                                                                                                                                                                                                                     ggACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCg                                                                                                                       <  2:1002918/183‑1 (MQ=255)                                                                                                                                                                                                                                                                  ccgcaaaCGGCGGGCGGG‑GCATTCACTTGGTGCCGCTGCTGCCGGGGGAGGCGGGGGACAGCCGCAGTGGGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCCCAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTgccgcc              <  2:76050/269‑1 (MQ=255)                                                                                                                                                                                                                                                                   cGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGGGTAAAGCCGCCGGGGGTCAATGTGGCCGGTGC‑GCCGTGGTAAGGCAGCGCAGCCGGACGGCCAAACACCGGCGCCGCTGGGGGGGGCGCCGCCGCCGGCGATCCGGTGAAACCCGCATCTTTACCTGGCGCCCAACAGCGCACCGGCGGCGCGGTGGGG‑ACTGGGGGGGTCTGGGGGGGTGCGGGGGGCGGAGCGCGGACTCACACcgccgcgggccccgg            >  2:1407247/1‑266 (MQ=255)                                                                                                                                                                                                                                                                      cgacgtagggcggA‑GCTTTCACATTGGGCCGCTGCCGCCGGGGGAGGCGGTGTACAGCCGCAGTGGGTCAATGTAGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTAGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTAc          <  1:198431/267‑1 (MQ=255)                                                                                                                                                                                                                                                                      cAACGGCGGGCGGA‑GCATTCACGTTGGGGCGCTGCTGCCGGGGGGGGCGGTGTACGGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTAcc         <  1:538268/275‑1 (MQ=255)                                                                                                                                                                                                                                                                       aaCGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGACGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTgggg                                                                   <  1:1011938/216‑1 (MQ=255)                                                                                                                                                                                                                                                                        aCGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCgg       <  1:915608/275‑1 (MQ=255)                                                                                                                                                                                                                                                                          ggcgggcgGG‑GCATTCACTTTGATCCGCTGCTGCCGGGTGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCTGCCGTACCggg      <  1:15326/274‑1 (MQ=255)                                                                                                                                                                                                                                                                              ggcggG‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACGGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCATCCCggtaagc  <  1:773254/274‑6 (MQ=255)                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                GGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCT  >  NC_000913/523345‑523886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑