| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 94.6% | 102.6 / NA | 37 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (14/21); minor base G (1/1); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132655‑2132925 | tggagGGTAAACAGCTGATAGTGTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:672151/141‑1 (MQ=255) gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCct > 1:402881/1‑143 (MQ=255) gagGGGAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct < 1:424153/143‑1 (MQ=255) caaCGGCTGATATTTTTTGCTCGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 1:331797/142‑1 (MQ=255) aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt > 1:250002/1‑143 (MQ=255) tGATCTTTTTTCCTGTCGCGGTCGCTTTTGTCTTTGTGCTTGCGGTAATAGTGGACGTACCCGGAGAATTTTTTCGGTGACGGGGTGATCTGCATTTCCCCGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa < 2:61770/143‑1 (MQ=255) tatTGTTTGCGTGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg < 2:709395/143‑1 (MQ=255) tgtGCTGGCGCGGTCGAGTGTGTCTTTGAGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc < 2:306606/141‑1 (MQ=255) gCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGt > 2:589085/1‑143 (MQ=255) tGGCGCGGTCGAATTGGTCTTTGTGCTTGCGGTAAAAGTGGACGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc < 2:264127/143‑1 (MQ=255) cGAACTGGTCTTTGGGCTTGCGGTAAAAGTGGAGGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 2:295696/143‑1 (MQ=255) aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGCAGGGTCTGGGTCCCCTATTCTACTTAGCAAGGTTTGCGGTACTCCACAGCCATc > 2:432115/1‑143 (MQ=255) tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc < 1:209769/143‑1 (MQ=255) ttGTGCTTGCGGTAAAAGTGGAAGCACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:567201/143‑1 (MQ=255) ggcaggTAAAGGTGGAAGTAGCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 2:626753/139‑1 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACCTTCCACGGTTCGCCGTACTCCCCCACCCTCCGCAGGCACACGTCGTaac > 2:290439/1‑142 (MQ=255) aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATcc > 1:286542/1‑143 (MQ=255) aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt > 2:5631/1‑143 (MQ=255) gTGGAAGTACCCGGGTAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCTGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg < 1:210075/143‑1 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGATAACGAGGTGAACAGCATAGCACCGTGAATGATATGCAGGCTCTGCGTCGCCTGTTCTACTTTCCACGGTGCGCCGGACACGACCCCCATCCGCCGGGAGACGTCGTAGTcatctg > 2:295311/1‑138 (MQ=255) aGTACCCGGAGAATTTTATCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt < 1:650131/143‑1 (MQ=255) gagaATTTTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACAGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc < 1:571964/143‑1 (MQ=255) agcagtgtttCGGTGACGGGGTGATCTGCATTTCACCGTGAGTGATCTGCAGGCTCCGCTCCGCCTCTTTTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCCTAATCCTGTGCCGCTTTCAGTTCg < 1:534885/136‑1 (MQ=255) aaTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGGCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGGTCTCAGCTCgga > 2:431684/1‑142 (MQ=255) tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGAAGGCGCGGCGTCGGCTCTTCTACTTTCCGCGGTTGGTCGTACTCTAGCACCATCCGCAGGAAGGTGTCGTAATGCGGTGCGCGTTACAGTGCGGtgta > 2:273203/1‑140 (MQ=255) ttttCGGTGACCAGGTGATCTGCATTCCACCGTGCTCGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGCTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 2:563542/143‑1 (MQ=255) cGGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca < 2:121774/143‑1 (MQ=255) ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaccaa > 1:597088/1‑143 (MQ=255) gTGCCGAGGTGATCTGCTTTTCACCGGGATTGATAGGCCGGCTCTGCGTCGCCGCTTCGACTTTCCACGGTCCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 2:269995/143‑1 (MQ=255) taCGAGCTTATCTGTATTTCAACGTGATGGAGATGCAGGGGCAGCGTCGCCTCTTCTACTGTCCACGGTGCGGCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTTCCGCTTTCAGTTCGGTATCGaacaaaca < 1:97961/142‑1 (MQ=255) gACGCGGTGTTCTGCATTTCACCGTGATTGATGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca < 2:196607/143‑1 (MQ=255) ccGAGGTGCTCTGCATTTCGCCGTGGTTGATATGTAGGCTCTGCGTCGCCTCTTCTACGTTCCGCGGTGCGCCGTAATCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac < 2:10247/142‑1 (MQ=255) ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATGCTGTGCCGCTTTCAGTGCGGCATCGAACAAACACTGtt > 1:716255/1‑143 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg > 1:496872/1‑143 (MQ=255) cctgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg < 2:321991/141‑1 (MQ=255) tatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAcc > 2:415314/1‑143 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGCAACGCCCCGCCCAGGTAAAGCCCCGGt > 2:75157/1‑143 (MQ=255) | TAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132655‑2132925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |