Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C93.3% 125.9 / 3.0 45intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base C (26/16);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 6.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
*NC_0009134,296,3802.G93.0% 123.6 / 2.7 45intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base G (25/15);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 6.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGT  >  NC_000913/4296179‑4296638
                                                                                                                                                                                                             ||                                                                                                                                                                                                                                                                    
aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGTTCGGATAAGGCGTTT‑‑ACCC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCCTCTTATCCGGCCTATCTTAACCGTTGGTTact                                                                                                                                                                                             >  2:407561/1‑273 (MQ=255)
 tGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGA‑TTTTTTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCCTCTTAACCGGCCTATCTTAACCCTTGGTTAAtt                                                                                                                                                                                            >  2:497145/1‑275 (MQ=11)
                     aTCGGGCTTAGGCAAGACGGCGCAATGGCCTGATTTAGCGTGAATTTGTAGGGAGGTTAAGGCGTTTATGCCGCAACCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg                                                                                                                                                                        <  2:72889/275‑1 (MQ=14)
                              aCGCCAGCCAGCGGAATAGCCGGATTTAGCGGTATTTTGTAGGTCGGATAAGGCGTGTAGGCCGCATCCTATAGCAACGCCTGATGCGACGCTTAACGCGTCTTAGCCGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTTTATGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                <  1:407562/274‑1 (MQ=14)
                              aCGCCAGACAGCGAGATGCCCTAGTTTGGCGTTATTTTGTAGGTCGGATAAGGCGTTGATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGTTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                <  1:151815/274‑1 (MQ=14)
                                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGCCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTCAACCCCGCTTTCAAACCCTTc                                                                                                                                                          >  2:229686/1‑274 (MQ=14)
                                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGACTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCCCCCGGTCTCAAACCCTTc                                                                                                                                                          >  2:140643/1‑274 (MQ=255)
                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTTCCGCGTCTTTTCAGGCCTCTCTTACCCGTTGGTTAATCTTTCAACCTCTTCACACCCGGTATCAACCCCTTCCATAc                                                                                                                                                     >  2:326027/1‑274 (MQ=14)
                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAGCGTTGGTTAATTTTTCAAACCTTTCCCACCCGGTCTCTAACGCTTCCATaaa                                                                                                                                                    >  1:70011/1‑273 (MQ=14)
                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTTTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GAATCCGACATCCATGCCTGATGCGACGCTTGACGCCTCTTAACCGCCCTATCTTACCCTTTGGCTAATTTTTCAAACTCCTCACACCCGGTCTCCAACCCTTCCCTAcc                                                                                                                                                    >  2:323888/1‑274 (MQ=14)
                                           cAATGGCTTGTTTGACCGTGATTTGGTAGGTCCGATAATGCGTTTATGCCGCAGCCGACATCAACGCCTGATGCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGA‑TTTTGGAGGTCGGATAAGGCGTTT‑‑ACTC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGc                                                                                                                                                  <  1:497146/275‑1 (MQ=17)
                                                   tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTCCCACACGGTATCAAACCCTTCCATCCAGccccgttt                                                                                                                                           >  2:489906/1‑267 (MQ=14)
                                                     aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTCGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa                                                                                                                                        >  2:441139/1‑275 (MQ=255)
                                                           cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTCGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATCCAGCCCAGGTTCCACCACTg                                                                                                                                  >  2:488095/1‑275 (MQ=255)
                                                              gagtttGTAGATCGGATAAGGCGTTTATGCCGCATCCGACATCAACGGCTGATTCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                                                               <  1:229686/272‑1 (MQ=255)
                                                                          cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAt                                                                                                                                                                    >  1:336331/1‑226 (MQ=255)
                                                                          cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTTCAGCGCCTTTTGCtt                                                                                                                   >  2:486130/1‑275 (MQ=255)
                                                                              tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                <  2:336330/226‑1 (MQ=255)
                                                                                aggctgtgaTGCCGCATCCGACATCAACGCCTGATTCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttctt                                                                                                             <  2:445304/267‑1 (MQ=255)
                                                                                  gcctttATGCCACATCCGACATCACCGCCTGTTGCGACGCTTATCCCGTCTTAGCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGGTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGGCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTtcttct                                                                                                           <  2:321966/272‑1 (MQ=21)
                                                                                      ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGTTTCAACCATTTCCGCCCCTCTTGCTTGtttttcactc                                                                                                        >  2:158043/1‑270 (MQ=18)
                                                                                       tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGAt                                                                                                       >  2:165409/1‑274 (MQ=255)
                                                                                       tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGAt                                                                                                       >  2:306690/1‑274 (MQ=255)
                                                                                                   gACAGCAACGCCTGATGTGACGTTTCACGAGTCTTCTCAGGCTAGGGCCAGAGATGGCAGTAGCCTGCTTTAGCGTTA‑GTTTGTTGTTCGGATGAGGCGTTTTCG‑‑C‑C‑GCAGCCGACATCAATGCCTGTGGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTGCCATACAGCTCAGGTTCATCCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCa                                                                                          <  2:302408/275‑1 (MQ=255)
                                                                                                          aCGCCCGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTA‑TTTTGTAGGTCGGCTAAAGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACGCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGttt                                                                                    <  1:202296/274‑1 (MQ=255)
                                                                                                           cGCCTGATGCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGAT‑AGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                                                                 <  1:184504/275‑1 (MQ=255)
                                                                                                               tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCTCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAc                                                                              >  2:328504/1‑275 (MQ=255)
                                                                                                                gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCATTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTCTTTCTCGATAAAACCTTCTTCACCGTTTAATAAca                                                                             >  1:202471/1‑275 (MQ=255)
                                                                                                                        gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTCGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTccc                                                                     <  1:488096/275‑1 (MQ=255)
                                                                                                                           tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                  <  1:260521/275‑1 (MQ=255)
                                                                                                                                 gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc                                                            <  1:130944/275‑1 (MQ=255)
                                                                                                                                   tttATCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATACAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTc                                                          <  1:357155/274‑1 (MQ=255)
                                                                                                                                           gCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGA‑TTTGGTAGGTCGGAGAAGGCGTTT‑‑A‑‑CGCAGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCTTACAGCTCAGGTTCACCCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCGTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc                                                  <  1:489907/275‑1 (MQ=37)
                                                                                                                                                          cgcAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAGTTg                                   >  2:509487/1‑275 (MQ=255)
                                                                                                                                                          cgcAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTg                                   >  2:498461/1‑275 (MQ=255)
                                                                                                                                                               tAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCaga                               >  1:67336/1‑274 (MQ=255)
                                                                                                                                                               aggCCTGATTTAGCGTGA‑TGTTGTAGGTCCGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCTACGCTTTCCTCGTCTTATCACGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCCACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCagag                              <  1:135848/273‑1 (MQ=38)
                                                                                                                                                                    tGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTTCAGCGCCTTTTGCTTGTTCTTCTCGATCAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAAACCTggc                         >  2:412595/1‑273 (MQ=255)
                                                                                                                                                                     gATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGACTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGATTGTTCTTCTCGATAAAACCTTCTTCACCGATGCAGTACATCATTCCCGCCCAGTTCTCGGAATAACCGGTTCGGGAAATGTCATAGCTGctt                        >  1:33141/1‑273 (MQ=37)
                                                                                                                                                                         tAGCGTGA‑TTTTGTATGTCGGATAAGGCGTTT‑‑A‑‑CGCAGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTg                     <  2:90603/274‑1 (MQ=255)
                                                                                                                                                                            cgggA‑TGTTGTAGGACGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAACCTTCCATACAGCTTAGGTTCAACCAGTCCAGCGCATTTTGCTTGTTCTTCCCGATAAAACCTTCTTCACCGTTTCAGAACATCCTTCCCGCCCAGTAGCCGGACTAACCGGTCCGGGAAATTga                                  >  2:135848/4‑257 (MQ=37)
                                                                                                                                                                            cGTGATTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGACCTGCGGTTGaaa                  >  2:212499/1‑275 (MQ=255)
                                                                                                                                                                                a‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                                                               <  2:67593/161‑1 (MQ=255)
                                                                                                                                                                                           tCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATCCCAGGTTCCCTTg   >  2:140998/1‑275 (MQ=255)
                                                                                                                                                                                            cGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCTCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCCGAGCTGCGTTTGAAATCCCAGGTTGCCtttt  >  2:357773/1‑273 (MQ=255)
                                                                                                                                                                                                             ||                                                                                                                                                                                                                                                                    
ATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGT  >  NC_000913/4296179‑4296638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑