breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 413,086:1 +G 5.9% coding (2668/3147 nt) sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 1,059,531 Δ1 bp 5.6% coding (276/2547 nt) torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
RA 1,261,890 G→A 12.1% intergenic (‑15/+136) prs ← / ← ispE phosphoribosylpyrophosphate synthase/4‑diphosphocytidyl‑2‑C‑methylerythritol kinase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,488,680 T→C 9.1% L150P (CTG→CCG)  aldA → aldehyde dehydrogenase A, NAD‑linked
RA 1,677,913 T→C 100% intergenic (‑10/‑514) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,032,556:1 +G 6.5% coding (524/696 nt) yedJ ← putative HD superfamily phosphohydrolase
RA 2,074,924 A→T 6.2% Y49F (TAT→TTT)  yeeR → CP4‑44 prophage; putative membrane protein
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,198,449 T→C 7.1% pseudogene (1976/2847 nt) yehH → DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function
RA 2,398,650 Δ1 bp 5.4% coding (1516/2727 nt) nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,437,246 A→T 8.9% I202I (ATT→ATA pdxB ← erythronate‑4‑phosphate dehydrogenase
RA 2,450,334 G→T 8.5% P86Q (CCG→CAG)  yfcP ← putative fimbrial‑like adhesin protein
RA 2,595,921 C→T 6.2% D273N (GAC→AAC)  ypfJ ← putative neutral zinc metallopeptidase
RA 2,773,070 A→G 8.5% pseudogene (113/117 nt) yfjV ← pseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein
RA 2,853,439 T→A 5.6% I62I (ATT→ATA hypE → carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein
RA 2,911,878:1 +A 7.4% coding (1774/2235 nt) relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 3,209,209 Δ1 bp 7.1% coding (1186/1464 nt) ttdT → L‑tartrate/succinate antiporte
RA 3,345,652:1 +G 5.1% coding (936/1434 nt) rpoN → RNA polymerase, sigma 54 (sigma N) factor
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,829,795 A→G 6.0% E284G (GAA→GGA)  xanP → xanthine permease
RA 3,850,850 A→G 6.4% N81N (AAT→AAC ilvN ← acetolactate synthase 1 small subunit
RA 3,899,745 A→G 7.3% L278P (CTT→CCT)  yieL ← putative xylanase
RA 3,950,342 Δ1 bp 100% coding (21/99 nt) ilvL → ilvG operon leader peptide
MC JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,084,689 G→A 5.0% N378N (AAC→AAT fdoG ← formate dehydrogenase‑O, large subunit
RA 4,094,415 T→G 14.5% intergenic (‑143/+308) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,244,371:1 +C 5.6% coding (705/1545 nt) malF ← maltose transporter subunit
RA 4,296,060 C→T 14.3% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→G 16.6% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 22.4% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,325,948 A→G 10.6% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,397,833 T→C 5.9% V141A (GTG→GCG)  mutL → methyl‑directed mismatch repair protein
RA 4,403,473 C→T 6.3% F58F (TTC→TTT hflC → HflB protease modulator specific for phage lambda cII repressor
RA 4,448,927 Δ1 bp 8.2% coding (236/351 nt) chpB → toxin of the ChpB‑ChpS toxin‑antitoxin system
RA 4,466,838 G→C 7.0% H137D (CAC→GAC)  treR ← trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor
RA 4,557,375 A→T 8.0% N128I (AAT→ATT)  iraD → RpoS stabilzer after DNA damage, anti‑RssB factor

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2099735 2101373 1639 12 [10] [10] 11 gnd gnd
* * ÷ NC_000913 3769583 3769899 317 12 [10] [9] 11 [yibV]–yibV [yibV],yibV

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 93647 =66 (1.320)4 (0.080) 4/474 NT 6.0% coding (482/1488 nt) murE UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase
?NC_000913 93928 = 62 (1.280)coding (763/1488 nt) murE UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase
* ? NC_000913 = 274456NA (NA)4 (0.080) 3/470 NT NA noncoding (694/1195 nt) IS5 repeat region
?NC_000913 = 274878 NA (NA)noncoding (272/1195 nt) IS5 repeat region
* ? NC_000913 315401 =NA (NA)13 (0.280) 9/462 NT NA noncoding (173/1255 nt) IS3 repeat region
?NC_000913 315627 = NA (NA)noncoding (399/1255 nt) IS3 repeat region
* ? NC_000913 = 315804NA (NA)5 (0.100) 4/476 NT NA noncoding (576/1255 nt) IS3 repeat region
?NC_000913 = 315887 NA (NA)noncoding (659/1255 nt) IS3 repeat region
* ? NC_000913 = 3814739 (0.180)10 (0.210) 5/466 NT 53.8% noncoding (214/1331 nt) IS2 repeat region
?NC_000913 = 381839 NA (NA)noncoding (580/1331 nt) IS2 repeat region
* ? NC_000913 387750 =54 (1.080)4 (0.080) 4/470 NT 7.7% coding (780/828 nt) tauC taurine ABC transporter permease
?NC_000913 387973 = 44 (0.920)coding (179/852 nt) tauD taurine dioxygenase, 2‑oxoglutarate‑dependent
* ? NC_000913 = 60596243 (0.860)4 (0.080) 4/476 NT 6.0% coding (238/249 nt) ybdJ DUF1158 family putative inner membrane protein
?NC_000913 = 606372 83 (1.710)coding (1012/1119 nt) ybdK weak gamma‑glutamyl:cysteine ligase
* ? NC_000913 631552 =62 (1.240)4 (0.080) 4/478 NT 6.5% coding (1659/2106 nt) cstA carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
?NC_000913 631690 = 54 (1.110)coding (1797/2106 nt) cstA carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
* ? NC_000913 729672 =61 (1.220)7 (0.150) 5/466 NT 10.7% coding (90/4194 nt) rhsC Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 729853 = NA (NA)coding (271/4194 nt) rhsC Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 762116 =70 (1.400)8 (0.170) 6/460 NT 9.4% coding (595/1218 nt) sucB dihydrolipoyltranssuccinase
?NC_000913 762340 = 88 (1.870)coding (819/1218 nt) sucB dihydrolipoyltranssuccinase
* ? NC_000913 = 108374254 (1.080)4 (0.080) 4/476 NT 6.9% coding (367/1272 nt) efeB deferrrochelatase, periplasmic
?NC_000913 = 1084288 56 (1.150)coding (913/1272 nt) efeB deferrrochelatase, periplasmic
* ? NC_000913 1207790 =28 (0.560)28 (0.600) 23/456 NT 53.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 23 (0.490)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780530 (0.600)31 (0.670) 30/456 NT 54.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 23 (0.490)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1251175 =42 (0.840)4 (0.080) 4/472 NT 8.5% coding (110/1920 nt) dhaR dhaKLM operon transcription activator
?NC_000913 1251499 = 46 (0.950)coding (434/1920 nt) dhaR dhaKLM operon transcription activator
* ? NC_000913 1264027 =51 (1.020)4 (0.080) 4/472 NT 8.1% coding (314/1257 nt) hemA glutamyl tRNA reductase
?NC_000913 1264913 = 42 (0.870)coding (1200/1257 nt) hemA glutamyl tRNA reductase
* ? NC_000913 1441369 =49 (0.980)4 (0.080) 4/476 NT 9.2% coding (375/423 nt) hslJ heat‑inducible lipoprotein involved in novobiocin resistance
?NC_000913 1441668 = 31 (0.640)coding (76/423 nt) hslJ heat‑inducible lipoprotein involved in novobiocin resistance
* ? NC_000913 1525564 =54 (1.080)5 (0.100) 5/476 NT 9.7% coding (417/1500 nt) ansP L‑asparagine transporter
?NC_000913 1525939 = 40 (0.820)coding (42/1500 nt) ansP L‑asparagine transporter
* ? NC_000913 = 162198648 (0.960)4 (0.080) 4/474 NT 7.5% coding (655/1188 nt) ydeE putative transporter
?NC_000913 = 1622325 52 (1.070)coding (994/1188 nt) ydeE putative transporter
* ? NC_000913 = 168532061 (1.220)6 (0.120) 6/472 NT 10.3% coding (1269/1404 nt) fumC fumarate hydratase (fumarase C),aerobic Class II
?NC_000913 = 1685547 46 (0.950)coding (1042/1404 nt) fumC fumarate hydratase (fumarase C),aerobic Class II
* ? NC_000913 = 182168734 (0.680)4 (0.080) 4/470 NT 9.3% intergenic (‑68/+231) chbB/osmE N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
?NC_000913 = 1822063 45 (0.940)coding (194/339 nt) osmE osmotically‑inducible lipoprotein
* ? NC_000913 1889160 =86 (1.720)4 (0.080) 4/474 NT 5.6% coding (587/1686 nt) fadD acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
?NC_000913 1889488 = 52 (1.070)coding (259/1686 nt) fadD acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
* ? NC_000913 = 200856662 (1.240)4 (0.080) 4/474 NT 6.2% coding (290/1206 nt) yedE UPF0394 family sulphur transport domain‑containing inner membrane protein
?NC_000913 = 2008914 60 (1.240)coding (638/1206 nt) yedE UPF0394 family sulphur transport domain‑containing inner membrane protein
* ? NC_000913 2179970 =50 (1.000)5 (0.100) 5/466 NT 10.0% coding (1150/1278 nt) yegT nucleoside transporter, low affinity
?NC_000913 2180269 = 42 (0.880)coding (175/1005 nt) yegU ADP‑ribosylglycohydrolase family protein
* ? NC_000913 2288919 =NA (NA)5 (0.100) 5/488 NT 12.5% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3968903 35 (0.700)coding (988/1104 nt) wecA UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
* ? NC_000913 2309320 =56 (1.120)3 (0.060) 3/466 NT 5.2% coding (22/651 nt) alkB oxidative demethylase of N1‑methyladenine or N3‑methylcytosine DNA lesions
?NC_000913 2309519 = 56 (1.180)coding (887/1065 nt) ada fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
* ? NC_000913 2326543 =42 (0.840)3 (0.060) 3/466 NT 6.7% coding (435/1185 nt) atoB acetyl‑CoA acetyltransferase
?NC_000913 2327148 = 43 (0.900)coding (1040/1185 nt) atoB acetyl‑CoA acetyltransferase
* ? NC_000913 2487645 =58 (1.160)4 (0.080) 4/478 NT 6.8% coding (3272/3594 nt) evgS hybrid sensory histidine kinase in two‑component regulatory system with EvgA
?NC_000913 2487739 = 52 (1.060)coding (3366/3594 nt) evgS hybrid sensory histidine kinase in two‑component regulatory system with EvgA
* ? NC_000913 = 253196536 (0.720)4 (0.080) 3/468 NT 6.0% coding (503/762 nt) cysZ sulfate transporter, sulfite inhibited
?NC_000913 = 2532167 91 (1.900)coding (705/762 nt) cysZ sulfate transporter, sulfite inhibited
* ? NC_000913 = 282844152 (1.040)3 (0.060) 3/474 NT 6.4% coding (705/780 nt) srlD sorbitol‑6‑phosphate dehydrogenase
?NC_000913 = 2828803 37 (0.760)coding (183/360 nt) srlM sorbitol=responsive srl operon transcriptional activator
* ? NC_000913 2895861 =49 (0.980)4 (0.080) 4/480 NT 8.0% coding (695/780 nt) ygcR putative flavoprotein
?NC_000913 2897818 = 44 (0.900)coding (53/1338 nt) ygcS putative MFS sugar transporter; membrane protein
* ? NC_000913 = 313541354 (1.080)5 (0.100) 4/470 NT 9.4% coding (959/1500 nt) pitB phosphate transporter
?NC_000913 = 3135486 44 (0.920)coding (886/1500 nt) pitB phosphate transporter
* ? NC_000913 = 314448060 (1.200)3 (0.060) 3/464 NT 5.6% coding (661/987 nt) hybA hydrogenase 2 4Fe‑4S ferredoxin‑type component
?NC_000913 = 3144983 45 (0.950)coding (158/987 nt) hybA hydrogenase 2 4Fe‑4S ferredoxin‑type component
* ? NC_000913 = 317075357 (1.140)5 (0.100) 3/470 NT 8.2% coding (270/1350 nt) qseC quorum sensing sensory histidine kinase in two‑component regulatory system with QseB
?NC_000913 = 3170894 57 (1.190)coding (411/1350 nt) qseC quorum sensing sensory histidine kinase in two‑component regulatory system with QseB
* ? NC_000913 3386475 =65 (1.300)5 (0.100) 5/470 NT 8.1% coding (1714/1968 nt) aaeB p‑hydroxybenzoic acid efflux system component
?NC_000913 3386687 = 51 (1.060)coding (1502/1968 nt) aaeB p‑hydroxybenzoic acid efflux system component
* ? NC_000913 = 358354411 (0.220)4 (0.080) 4/482 NT 35.0% noncoding (117/768 nt) IS1 repeat region
?NC_000913 = 3583630 4 (0.080)noncoding (203/768 nt) IS1 repeat region
* ? NC_000913 3620750 =NA (NA)3 (0.060) 3/484 NT 5.3% coding (1559/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3621165 = 54 (1.080)coding (1974/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 367413956 (1.120)4 (0.080) 4/468 NT 7.6% coding (778/1014 nt) yhjD inner membrane putative BrbK family alternate lipid exporter
?NC_000913 = 3674303 43 (0.900)coding (942/1014 nt) yhjD inner membrane putative BrbK family alternate lipid exporter
* ? NC_000913 = 387344549 (0.980)4 (0.080) 4/466 NT 6.8% coding (168/618 nt) dgoA 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
?NC_000913 = 3873720 63 (1.320)coding (755/879 nt) dgoK 2‑oxo‑3‑deoxygalactonate kinase
* ? NC_000913 = 392799358 (1.160)3 (0.060) 3/474 NT 5.3% coding (839/993 nt) asnA asparagine synthetase A
?NC_000913 = 3928225 51 (1.050)coding (1379/1452 nt) viaA stimulator of RavA ATPase activity; von Willebrand factor domain protein
* ? NC_000913 = 412544654 (1.080)4 (0.080) 3/464 NT 6.8% coding (1365/2199 nt) priA Primosome factor n' (replication factor Y)
?NC_000913 = 4125678 59 (1.240)coding (1133/2199 nt) priA Primosome factor n' (replication factor Y)
* ? NC_000913 = 426395560 (1.200)5 (0.100) 5/480 NT 7.8% coding (277/984 nt) qorA quinone oxidoreductase, NADPH‑dependent
?NC_000913 = 4264427 59 (1.200)coding (114/1416 nt) dnaB replicative DNA helicase
* ? NC_000913 = 427916876 (1.520)4 (0.080) 4/468 NT 5.3% coding (690/1350 nt) ghxP guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
?NC_000913 = 4280191 69 (1.440)coding (212/1650 nt) yjcE putative cation/proton antiporter
* ? NC_000913 = 434962275 (1.500)5 (0.100) 4/472 NT 7.5% coding (413/720 nt) dcuR response regulator in two‑component regulatory system with DcuS
?NC_000913 = 4349717 51 (1.060)coding (318/720 nt) dcuR response regulator in two‑component regulatory system with DcuS
* ? NC_000913 4410256 =46 (0.920)4 (0.080) 4/470 NT 9.8% coding (124/402 nt) yjfI DUF2170 family protein
?NC_000913 4410527 = 29 (0.600)coding (395/402 nt) yjfI DUF2170 family protein