Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,473,884 | 0 | T | G | 24.6% | 87.3 / 10.3 | 61 | L94R (CTG→CGG) | yfdP | CPS‑53 (KpLE1) prophage; uncharacterized protein |
Reads supporting (aligned to +/- strand): ref base T (21/25); new base G (15/0); total (36/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.28e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CTCAACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGT > NC_000913/2473742‑2474020 | ctcaACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCt < 1:15115/143‑1 (MQ=255) tCGAACCATTTGATGAGAGCCGGCTGAAACTCAAACACAAACTCTCATAGGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAg < 1:158627/143‑1 (MQ=255) tCGAACCATTTGAGGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAg < 1:783215/143‑1 (MQ=255) gAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGa > 1:975694/1‑143 (MQ=255) gAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGAATAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTTACTATTCTTCAGGa > 2:900170/1‑143 (MQ=255) tgatgtgaagaGAAGCGTCTGAAGATCGAACACAAACGCGCAGATGGTCGCCCGACTAACCGTGGGACGATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGa < 1:1053420/138‑1 (MQ=255) tgatgaGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAgg < 1:992112/143‑1 (MQ=255) gatgaGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGACGAAGACACCACCGAAACGCCGATGTATGGCAATCGCGGTGGTCGCCTGACCATTCTGCAGGAAGACCAggt > 2:953908/1‑142 (MQ=255) gatgaGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCTCGGTGGTCGCCTGACTATTATGCAAGAAGACCAggg > 2:468644/1‑143 (MQ=255) atgaGAACCGTCGGAAAGTCAACCACAAACTTCGATATGTGCTCCCGTGAAACCGTGGGAAAATTTCCGACGCAGACACCACCGAACCGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGa < 2:753500/143‑1 (MQ=255) gagaACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACCATTCTAAAAGAAGACACGGGACa > 2:738304/1‑143 (MQ=255) tGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCGGACCATTCTGCAAAAAAACCAGGCACAATTACTGCct > 2:545480/1‑143 (MQ=255) aTCAAACACAAACTCTCGTACGTTCGCCCGACTGACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGc < 2:910736/143‑1 (MQ=255) aTCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCGTACTATTCTGCAGTAAGACCAGCGACAATTACTGACTCTTGc > 2:444022/1‑143 (MQ=255) cAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGACGACCAGGGACAATTACTGACTCTTGCCg > 1:1099465/1‑143 (MQ=255) cAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCg < 1:130999/143‑1 (MQ=255) aaCACAACCTCTCATTTGTCCGCCCGACTAACCGTGGGACAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTTTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGt < 1:607635/143‑1 (MQ=255) aaCACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCGGACCATTATACAGGAAGACAAGCGACAATTCCTGACTCTTGCCGGt > 2:838986/1‑143 (MQ=255) cacaAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGa < 1:949980/143‑1 (MQ=255) aaaCTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGCGcc > 1:172881/1‑143 (MQ=255) tcATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCGGACCATTCAGCAAGAAGACCAGCGACACTTACTGACTCTTGCCGGGGAGCCTGCCgg > 2:727072/1‑143 (MQ=255) tcATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCACGGTGGTCGCCTGACTATTCTGCACGAAGACCAGGGACAATTACTGACTCTTGCCGGCGAGCATGACgg > 1:451332/1‑143 (MQ=255) ttttGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGaa < 1:1157497/140‑1 (MQ=255) cgatGTGCGGCAGACGAGCCGTGGGTAAATTTCGGAAGCAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGaa < 2:795124/141‑1 (MQ=255) ccGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAAGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGACAACCTCCgcgc > 2:43309/1‑143 (MQ=255) gTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGACAACTCCGCGCCGCAGGTcgt > 1:1137006/1‑141 (MQ=255) gAAAATTTCCGAAGAATACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAat < 1:549363/143‑1 (MQ=255) gAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCGGACTATTCTGCAAGAAAACCAGCGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCCCAGCCGGTCATTact > 2:863151/1‑141 (MQ=255) aaaaTTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAata > 1:354937/1‑143 (MQ=255) aaTTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATc < 1:1146312/143‑1 (MQ=255) aTTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCGGACCATTCAGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGCAAACTCCGCGCAGCAGGTCATTAATATcc > 2:548566/1‑142 (MQ=255) tttCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACCATTCTGCAGGAAGACCAGGGGCAATTACTGACTCTTGCCGGTGAGCCTGACGGAACACTCCGCGCAGCAGGTCAGTAATATCAt > 2:846379/1‑143 (MQ=255) tttCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCGGACCATTCAACAAAAAGACCAGGGACAATTACTGACTCATGCCGCTGAGCCTGCCGGAAAACTCCGCGCAGCCGGCCCTTAATATCac > 2:957021/1‑142 (MQ=255) ttCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCAtt < 2:1051544/143‑1 (MQ=255) tcccGAACAAGACACCACCGAATCGCCGATGTATGTCAATCGCGGTGGCCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACCCCGCGCAGCAGGTCATTAATATCAtt < 1:805212/141‑1 (MQ=255) gaagaaGACACCACCGAAACGCCGATGTATGTTAATCGCGGTGGTCGCCTGACCATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCtta > 2:893825/1‑143 (MQ=255) gcggcaaccaccGGTGCGCCGATGTGTGCCAATCGCGGTGGCCGCCTGACTAGTCTGCAGGAAGACCAGGGACAATCACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCttaatt < 2:663938/137‑1 (MQ=255) gaagaCACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACCATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCttaatt > 2:910395/1‑143 (MQ=255) gaagaCACCACCGAAACGCCGATGTATGTCAAGCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCttaatt < 1:953908/143‑1 (MQ=255) gaCACCACCGAAACGCCGATGTATGTCAATCTCGGTGGTCGCCGGACCATTCTACAGAAAAACCAGAGACAATTACTGACTCTTGCCGGTGAACCAGACGGAAAACTCAGCGCAGCAGGTCATTAATATCATTCTTAATTAAc > 2:598485/1‑143 (MQ=255) gaCACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAATTTCGCGCAGCAGGTCATTAATATCATTCTTAATTAAc > 1:98329/1‑143 (MQ=255) caccGAAACGCCGATGTATGTCAATCTCGGTGATCGCCGTACCATTCAACACAAAGACCAGGGACAATTACGGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCAGTAATATCATGCTTAATTAACTaaat > 2:615475/1‑141 (MQ=255) accGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCGGACCATTATGCAAGAAGACCAGGGACAATTACTGACTCTTGCAGGTGAGCCTGGCGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATAAAATAatta > 2:56684/1‑143 (MQ=255) accAAAACGCCGACGTCTGTCAATGGCGATAGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAACACTCCGCGCAGCAGGTCATTACTATCATTCTTCCTTAAATAatta > 1:548430/1‑143 (MQ=255) ccGAAGCGCCGATGTATGTCAAGCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAattat < 1:304274/143‑1 (MQ=255) ccGATGTATGTCAATCTCGGTGGTCGCCGTATCATTAAACAAAAAAACCAGCGACAATTACTGACTCTTGCCGGTGAGCCTGACGAAAAACTCCGCGCACAAGGACATTAACATCATCCACAATTAACTAAttatttatacca > 2:161962/1‑139 (MQ=255) cGATGTATGTCAATCGCGGTGGTCGCCTGACGATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCtcat < 1:530172/143‑1 (MQ=255) gATGTATGTCAATCTCGGTGGTCGCCGGACTATTCAACAAGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTACGAGCAGCAGGGCATTCACAACATACTTAATTAACTAATTAGTTAGCgcatc > 2:217876/1‑143 (MQ=255) gtatgtCAATCGCGGTGGTCGCCTGATTATTAAAAAGAAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTAATCAca > 2:536872/1‑142 (MQ=255) tatgtCAATCTCGGTGGTCGCCGGATCATTAAAAAAAAAAACCAGGAACAATTACTGACTCTTGCCGGTGAACCTGACGGAAAACTCCGCGCAGCAAGACATTAATATCAATCTTAAATAACTAATTAATTatcacatcacaa > 2:345216/1‑141 (MQ=255) atgtCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGAGGAGCTTGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGa > 1:1166206/1‑143 (MQ=255) tgtCAATCGCGGTGGTCGCCGTACCATTCTACAAAAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTACCTCATCACTGaa > 2:1003800/1‑143 (MQ=255) cgtCACTCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTGCTGACTCTTGCCGGTGAGCCTGACGGAAATCTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGaa < 2:578565/142‑1 (MQ=255) tCAATCGCGGTGGTCGCCTCACTATTAAGAAGGAAGACCTGGGACAATTACTGACTCTTACAGGTAGGCCTGACGGAAAACTCCGCGCAGCAGGTGAATAATATCATTCTTAAGTAAACAATTATCTATATcaccaccaaatc > 2:528012/1‑137 (MQ=255) tCAATCGCGGTGGTCGCCGGATTATTAAGAAAAAAAACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTAAGCGCAGCAGGTCATTAATATCATTCTTAAATAACTAATTAATTAACTCATCACTGAata > 2:1109125/1‑143 (MQ=255) cAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAatat < 1:1034446/143‑1 (MQ=255) aTCGCGGTGAACGCCTGACTATTCTGCAGGAAGAACAGGGACACTTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCt < 2:745730/143‑1 (MQ=255) gcgGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTaa < 2:12539/143‑1 (MQ=255) cgGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTCGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAat < 1:1066984/143‑1 (MQ=255) gtCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCCTCGCGGAATATCTTAATATAGt > 1:280524/1‑143 (MQ=255) gtCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGt > 1:81163/1‑143 (MQ=255) gtCGCCTGACTATTCGGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGt < 1:43309/143‑1 (MQ=255) | CTCAACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGT > NC_000913/2473742‑2474020 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |