Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 699,471 Δ1 bp intergenic (‑294/+103) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913699,4680A.97.9% 182.2 / ‑3.4 48intergenic (‑291/+106)asnB/umpHasparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (21/26);  minor base G (1/0);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACG  >  NC_000913/699327‑699602
                                                                                                                                             |                                                                                                                                      
aGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGCACGCCTTATCCGGCCTACAAAAGCAGGaa                                                                                                                                       >  1:415098/1‑143 (MQ=255)
     atatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATGTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaaattca                                                                                                                                  <  1:45523/143‑5 (MQ=255)
      tatttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCaa                                                                                                                                <  1:605081/143‑1 (MQ=255)
      gagttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCaa                                                                                                                                <  1:58476/140‑1 (MQ=255)
       atttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAat                                                                                                                               >  1:323453/1‑143 (MQ=255)
       atttattGAGGAATCGATAATTGTTAGCGGGTTTTATGGACTGTTTCTATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAat                                                                                                                               <  2:628073/143‑1 (MQ=25)
             tgatgcATCGATAAGTTGTAGCGGGTTTGATTGAATGTTTATATTTGACGGGGGCCAAATTGCTGACAAAGTTCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTg                                                                                                                         <  1:570749/143‑1 (MQ=25)
             tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAACGCATGAAATTCAATATATTg                                                                                                                         >  1:541462/1‑143 (MQ=255)
               atgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCa                                                                                                                       >  1:181712/1‑143 (MQ=255)
               atgaATCGATAATTTTTAGCGGGTTTTATTGAATGTGCATAGGGGACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCa                                                                                                                       >  1:693951/1‑143 (MQ=25)
                tgaATCGATAATTTGTAGCGGGTTTTAGTGAATGTTTATCTTTTACGGGGGCCAAATTGCTGACATAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAg                                                                                                                      <  2:303238/143‑1 (MQ=25)
                    tCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGAAGGAGc                                                                                                                  >  2:554559/1‑143 (MQ=255)
                       aTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGAGCGATTTGTTCATGCCGGATGCGGCGTGAACGACTTATCCGGCCTACAAAAGCACGGAGATCAAGATATTGCAGGAGCTGc                                                                                                               >  2:472203/1‑143 (MQ=25)
                             tttAGCGGGTTTTATTGAGTGTTTATGTTTTATGGGGGCCACATTGCGGACAAATTGCGATTTGTTCATGCCTTATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGc                                                                                                         <  2:627775/143‑1 (MQ=25)
                             tttAGCGGGTTTTATTGAATGTTTATATGGTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGc                                                                                                         <  1:612787/143‑1 (MQ=255)
                             ttgggCGGGTTTGATTGAGTGTTGGTATTTGACGGGGGCGAAGTTGCTGACAGAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACAGAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGc                                                                                                         <  2:27034/139‑1 (MQ=18)
                                  cGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGCGCCGCGTAGGCCTGAt                                                                                                    >  2:540799/1‑143 (MQ=255)
                                         tATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTa                                                                                             <  1:297039/143‑1 (MQ=255)
                                           ttGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgc                                                                                           <  1:429898/143‑1 (MQ=255)
                                             gAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGACATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgcgc                                                                                         >  1:474566/1‑143 (MQ=255)
                                              aaTGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGAGAAGAGTAGTGCa                                                                                        >  2:413990/1‑143 (MQ=255)
                                                tGTTTAGATTTTACGGGGGTCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGGCTACAAAAGCATGAAATTCAATATATTGCATGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc                                                                                      <  2:20546/143‑1 (MQ=18)
                                                   ttATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCTCATCAgg                                                                                   <  2:484113/143‑1 (MQ=255)
                                                       atTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGt                                                                               <  2:534542/143‑1 (MQ=255)
                                                             cGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCg                                                                         <  2:181712/143‑1 (MQ=255)
                                                                gggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGttt                                                                      <  1:337592/143‑1 (MQ=255)
                                                                gggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGttt                                                                      <  1:306086/143‑1 (MQ=255)
                                                                 ggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTg                                                                     <  2:228417/143‑1 (MQ=255)
                                                                  gCCAAATTGCTGACGAAGTGGGATTTGTTCGTGTCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATGGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGt                                                                    <  2:40520/143‑1 (MQ=18)
                                                                   ccAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCAACAGGCAGGTTGACgctggac                                                                   >  2:562641/1‑137 (MQ=18)
                                                                         tGCTGATAGAGTGCGATTTGTTAAGGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCaga                                                             <  2:204406/143‑1 (MQ=18)
                                                                            tGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGcc                                                          >  1:73195/1‑143 (MQ=255)
                                                                              aCAAAGTGCGGTTGGTTCAGGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCaa                                                        <  1:299925/143‑1 (MQ=18)
                                                                                aaaGGGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAAcc                                                      <  2:272884/143‑1 (MQ=255)
                                                                                                aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTgg                                      >  1:149828/1‑143 (MQ=255)
                                                                                                 tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGt                                     <  1:320144/143‑1 (MQ=255)
                                                                                                   ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTg                                   >  1:346222/1‑143 (MQ=255)
                                                                                                   ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAGTCAAAATATTGCAGAAGCTGCGTAAGACTGATAAGCATAGCGCATCAGGCAGTTTGGCGATAGAAAACAGAGCCAACCACGTCCACAAACGTGGGTg                                   >  2:269353/1‑143 (MQ=17)
                                                                                                        tGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTAtt                              <  1:554559/143‑1 (MQ=255)
                                                                                                        tGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGATGCAAtt                              >  1:397176/1‑143 (MQ=255)
                                                                                                           ggcgTGAACGCCTTATCCGGCCTACAAACGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAg                           <  1:415306/143‑1 (MQ=255)
                                                                                                               tGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATaa                       >  2:599588/1‑143 (MQ=255)
                                                                                                               tGAACGCCTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATaa                       >  1:519397/1‑143 (MQ=255)
                                                                                                                    gCCTTATCCGGCCTACACAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCg                  >  2:90830/1‑143 (MQ=255)
                                                                                                                      cTTATCCGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCACAGACGTGGTTGCTCTTCAGGCAACGTCGAt                >  2:584201/1‑143 (MQ=25)
                                                                                                                      cTTACCCGGCCGACAAAGGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAt                <  2:571017/143‑1 (MQ=255)
                                                                                                                            cGGCCTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc          <  2:323453/143‑1 (MQ=255)
                                                                                                                                cTACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACt      >  2:200895/1‑143 (MQ=255)
                                                                                                                                 tACAAAAGCATGAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGAGAACGTCGATTTCAGCGACTg     >  2:231125/1‑143 (MQ=255)
                                                                                                                                    aaaaGCATGAAATTCAATATATTGCCGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGGTTGGCGCTTGCCACCAGGACTGGCTAGGTCTGCAGGCGTGGTTGCTCTTCAGCTACCGTCGCTTTCAGCGACTGCCg  >  1:166114/1‑143 (MQ=12)
                                                                                                                                             |                                                                                                                                      
AGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACG  >  NC_000913/699327‑699602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: