Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4001645 4001649 5 36 [0] [0] 35 corA magnesium/nickel/cobalt transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40016440 (0.000)35 (0.870) 32/284 0.3 100% coding (219/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913 4001650 = 0 (0.000)coding (225/951 nt) corA magnesium/nickel/cobalt transporter

TTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCC  >  NC_000913/4001511‑4001789
                                                                                                                                           |                                                                                                                                           
gcagtttgctctcgtcgaagctgacaacgccgagcgactgctcgtacaatctgaacttggccagagcctggcaccccgcccggaactggaatacatcgaagcatcggtacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                          <  2:212600‑M2/4‑1 (MQ=255)
    attactcttgtctacccggacgacgacgagcgactgcgcgtccaacccgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttct                                                                                                                                      <  1:345550‑M2/8‑1 (MQ=255)
         tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttc                                                                                                                                 >  1:424931‑M2/131‑143 (MQ=255)
              ccgaaccggacgacggcgagcgactgcgcgtacaatctgaacctggccagagcctggcagcccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGa                                                                                                                            <  1:464990‑M2/18‑1 (MQ=255)
               cgacccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGaa                                                                                                                           <  1:81461‑M2/19‑1 (MQ=255)
               cgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGaa                                                                                                                           <  1:581910‑M2/19‑1 (MQ=255)
                 aaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGa                                                                                                                         <  1:238631‑M2/21‑1 (MQ=255)
                        cgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGaa                                                                                                                  <  1:352261‑M2/28‑1 (MQ=255)
                               gagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTCCTTTGAAGATGCGGAAGATCACg                                                                                                           >  2:466608‑M2/109‑143 (MQ=255)
                               gagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTCCTTCTCTGAACATGCGGAACGTCACg                                                                                                           >  2:584716‑M2/109‑143 (MQ=255)
                                   gactgcgcggacaagctgaagttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCgg                                                                                                       <  1:316637‑M2/39‑1 (MQ=255)
                                         gcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGACGCTGCGGACGGTCACGCCGGTAGctg                                                                                                 >  1:296069‑M2/99‑142 (MQ=255)
                                                atctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgtg                                                                                          <  1:237793‑M2/52‑1 (MQ=255)
                                                  ctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGTGGAAGATCACGCCGGTAACTCCACTGTGGc                                                                                        >  1:395295‑M2/90‑143 (MQ=255)
                                                   tgaacttggccagagcctggcaacccgcccggaactggaagacatcgcagcatcggcacgtttcttggaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGAGGCGGAAGATCACGCCGGTAAAgccactgagcgc                                                                                       >  1:670761‑M2/89‑138 (MQ=255)
                                                            cccgagcctggcaacccgcccggaactggacgccatcgacgcatcggcacgtttctttgaagacgacgaccgcctgcatCTCCTTCTTCTTCTTTGCAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATc                                                                              <  1:176229‑M2/64‑1 (MQ=255)
                                                             cagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATcc                                                                             >  2:517973‑M2/79‑143 (MQ=255)
                                                               gagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGt                                                                           <  1:176889‑M2/67‑1 (MQ=255)
                                                                        aacccgcccggaactggaagacatcgaagcatcggcacggttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTGGAAGATGCGGAAGATCACGCCGGTAACTCCACTCTGGCATTTACCATCCGTGATGgtcgt                                                                  <  2:694786‑M2/76‑1 (MQ=255)
                                                                          cccgcccggagcgggaagacatcgaagcatcggcacgtttcttttaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCt                                                                <  2:402298‑M2/78‑1 (MQ=255)
                                                                                  gaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGCGATGGTCGTCTGTTTActc                                                        >  2:531430‑M2/58‑143 (MQ=255)
                                                                                   aactggaagacagcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTActct                                                       <  1:111158‑M2/87‑1 (MQ=255)
                                                                                       ggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgt                                                   <  1:13540‑M2/91‑1 (MQ=255)
                                                                                       ggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCGTCTTTGAAGATGCGGAAGATCACGCCGGTACCTCCGCTGTGGCATTTGCCATCCGTGATGGTCGGCTGTTTACTCTgcgt                                                   >  1:549282‑M2/53‑143 (MQ=255)
                                                                                           gacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagc                                               >  1:252733‑M2/49‑143 (MQ=255)
                                                                                              atcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTC‑‑‑CTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATACGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAAc                                         >  1:177761‑M2/46‑143 (MQ=255)
                                                                                                          gcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGTGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCtt                                <  2:395295‑M2/110‑1 (MQ=255)
                                                                                                          gcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCtt                                <  2:675406‑M2/110‑1 (MQ=255)
                                                                                                                  ctttgaagacgacgccggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTACCTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTg                        <  1:315235‑M2/118‑1 (MQ=255)
                                                                                                                   tttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTCTCGGCTGt                       >  1:177128‑M2/25‑143 (MQ=255)
                                                                                                                          acgacgtcggcctgcatCTCCTTTTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTATTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAt                <  1:696586‑M2/126‑1 (MQ=255)
                                                                                                                            tgcgtcggcctgcatCTCCTTCTTCTGCCTTGACGTTGCGGCAGCTCTCGCCGGTACCTCCTCTGTGGCACTTACCCTCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGc              <  2:722643‑M2/128‑1 (MQ=255)
                                                                                                                                gcggcatgcatCTCCTTCTTCTTCTCTGAAGATGCGGAAGATCACGCCGGTAACTCCCCTGTGGCATTTACCATCCGTGATGGTCGTTTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGc          <  1:145406‑M2/132‑1 (MQ=255)
                                                                                                                                       gcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGCGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGCGCCCGTAGc   >  2:615002‑M2/5‑143 (MQ=255)
                                                                                                                                        catCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAAATATTCGCTGCCCGTCTGTATCGTATGCGTGCCCGTTGcc  >  1:302224‑M2/4‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCC  >  NC_000913/4001511‑4001789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.