Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,678,024 C→T intergenic (‑121/‑403) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,678,0240CT97.2% 98.1 / ‑6.7 38intergenic (‑121/‑403)pntA/ydgHpyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (19/16);  minor base G (0/1);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

TTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAAT  >  NC_000913/1677885‑1678150
                                                                                                                                           |                                                                                                                              
ttGGTATTCCAAGTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTATATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTa                                                                                                                             <  2:57819/143‑1 (MQ=255)
ttGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTa                                                                                                                             <  2:601127/143‑1 (MQ=255)
ttGGTACGCCAATTCGCATAACATTCCCTTCCATCGGTTTTATTGCGGAAAGTATGCCCTCGTCCCCTGCCCCACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTa                                                                                                                             <  2:515452/143‑1 (MQ=255)
     aTGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGaa                                                                                                                        <  1:63335/143‑1 (MQ=255)
          aaTTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACACACGCCCTATAATGTACTGAAAATTa                                                                                                                   >  1:494511/1‑143 (MQ=255)
          aaTTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTa                                                                                                                   >  1:494505/1‑143 (MQ=255)
          aaTTCGAATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGACACACACGCTGCTCATGTACGAGCTAAATGGGCCTCCGTGAAACAAAATTCGCAACAAACGCCATAGAAAGTACTGAACATTa                                                                                                                   >  1:494506/1‑143 (MQ=255)
                cATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCtg                                                                                                             >  1:702789/1‑143 (MQ=255)
                cATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCAtg                                                                                                             >  1:464469/1‑143 (MQ=255)
                   gATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCGCTATAATGGACTGAAAATTATGACTGTGa                                                                                                          >  2:459706/1‑143 (MQ=255)
                    atatTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTCCTGAAAATTATGCCTGTGAt                                                                                                         >  2:184184/1‑143 (MQ=255)
                         cccTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAgc                                                                                                    <  1:480657/143‑1 (MQ=255)
                               cATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCaaa                                                                                              >  1:638321/1‑143 (MQ=255)
                                              tgatgGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACACAGGGGTAATATATTGGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGc                                                                               >  1:103321/1‑143 (MQ=255)
                                                          tgtgTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCATTATTTAGGCGTGAACTCGCaa                                                                   >  1:5318/1‑143 (MQ=255)
                                                                  gAGCCACACAAGCTGCTTATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTaa                                                           <  2:227282/143‑1 (MQ=255)
                                                                    gCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTTAACTCGCAAAATCTTAAcc                                                         >  2:229015/1‑142 (MQ=255)
                                                                       acacaAGCTGCTCATGTACGAGCTAACTGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGAtt                                                      >  1:138827/1‑143 (MQ=255)
                                                                       acacaAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGAtt                                                      >  1:186850/1‑143 (MQ=255)
                                                                              atgctCATGTACGAGCTAAATTTTACTCCTTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGt                                               <  2:309264/142‑1 (MQ=255)
                                                                                gctCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAAGGTGCAGCCgaga                                             >  1:284469/1‑143 (MQ=255)
                                                                                 ctCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAACATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGAt                                            >  1:7245/1‑143 (MQ=255)
                                                                                    aTGTAACAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGt                                         <  2:46327/143‑1 (MQ=255)
                                                                                      gTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTgg                                       <  1:651007/143‑1 (MQ=255)
                                                                                       tACGAGCCAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGa                                     >  2:607168/1‑143 (MQ=255)
                                                                                       gaCGAGCTAACTGTTAAGCCGTTAAAATAAATGAGTAACAAACGCCCTCTAATGTTCTGAAAAGTATGCCGGTGATGTAGCGCCAAAAAGCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTggg                                      <  2:435528/142‑1 (MQ=255)
                                                                                         cGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGAt                                    <  2:473004/143‑1 (MQ=255)
                                                                                              tAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAGATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAaca                               <  2:47355/143‑1 (MQ=255)
                                                                                              tAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAaca                               >  1:348265/1‑143 (MQ=255)
                                                                                               aaaTGGTACTCCGTTAAAAGAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAacac                              <  1:658803/143‑1 (MQ=255)
                                                                                                aaTGTTACGCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTAGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACAcc                             >  2:296272/1‑143 (MQ=255)
                                                                                                     tACTCGGTTAAAATAAATTAGTAACAAACGCCCTATACTGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCCGCGTGAACTCGCAACATGTAGAGAATTGAGCCGTGATCGTGGGATAAACACCTTAGa                        >  2:149479/1‑143 (MQ=255)
                                                                                                      aCTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGaa                       <  1:107250/143‑1 (MQ=255)
                                                                                                                  taaatTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGACAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACAGCTTAGAACGCCGGATAAAg           >  2:458420/1‑143 (MQ=255)
                                                                                                                    aatTAGTTACAAACGCCCTACAATTTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGAGTGACCTCGCAAAATATTAACGATGCAGCCGTGATAGTGGGGTAAACACCTTAGACCGCCGGATAAAgtc         <  2:101726/143‑3 (MQ=255)
                                                                                                                          tAACAAACGCCCTACAAGCTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATaa   <  2:391417/143‑1 (MQ=255)
                                                                                                                           agcAAACGCCCTATAATGTTCTGTAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAAt  <  2:675420/141‑1 (MQ=255)
                                                                                                                           aaCAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAAt  <  1:574899/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                              
TTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAAT  >  NC_000913/1677885‑1678150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: