Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,375	T→C	Q222Q (CAA→CAG)	hdhA ←	7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,375	0	T	C	100.0%	84.2 / NA	29	Q222Q (CAA→CAG)	hdhA	7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

CAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGG  >  NC_000913/1697238‑1697513                                                                                                                                          |                                                                                                                                           caacaCGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGAAAACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCgg                                                                                                                                       >  1:590243/1‑143 (MQ=255)              tgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGt                                                                                                                          >  1:461499/1‑143 (MQ=255)               gttTACCGGTCCGTTAGTGTATTACTTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCCCCCACCCCGCCCGCACCCGGCGACCAACGACACATCGCTTCCTAAGCACTATCCTGCCGTTGGcccccacccc                                                                                                                         >  2:478116/1‑136 (MQ=255)                   aCCGGCCCGTTAGTGCATTAATTGAGCTCCTGGACCCCACCACCGTAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGAt                                                                                                                     <  1:160158/143‑1 (MQ=255)                       gtcctgtAGCGTATTAGTCTAGATCCTGTACCCCACCACCGGAGACGGTGAGAATCTCTCTGCGTCCGCAGCTCTCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGc                                                                                                                 <  2:436505/137‑1 (MQ=255)                            ttAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGCCGGTGAGCATTTGTCCACTTAAACAGCTCGCAACAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGagtg                                                                                                            >  1:30604/1‑143 (MQ=255)                            ttAGCGTATTAATTGAGCTCCTGTACCCCACCCCCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGtgtg                                                                                                            <  1:317738/143‑1 (MQ=255)                                  tATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGGGAGAATGCGGCCGCTTACCCAGCTCGCAGCAGGCGCGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCCGACGTCTGATAGGCGTGTCCTggca                                                                                                      >  2:495979/1‑140 (MQ=255)                                     tAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACAt                                                                                                   >  1:123900/1‑143 (MQ=255)                                             cTCCTGTACCCCCCCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGtt                                                                                           <  2:276887/143‑1 (MQ=255)                                                      cccACCACCGGAGACTGTGAGAATTTGGCCCCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTg                                                                                  <  1:437322/143‑1 (MQ=255)                                                      cccACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTg                                                                                  <  1:550655/143‑1 (MQ=255)                                                      cccACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTg                                                                                  <  1:277141/143‑1 (MQ=255)                                                      cccACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATCTTTTGTTCAAGTTCTg                                                                                  >  2:38788/1‑143 (MQ=255)                                                                        gagaATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCCGACGTCTGATCGACGTGTGCTGTAACATTTTTTCTTCAATTGCTCATGTAGTAACGGATTTcg                                                                >  2:620187/1‑142 (MQ=255)                                                                          gaATTGGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAgg                                                              <  2:682507/143‑1 (MQ=255)                                                                               tGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATc                                                         <  2:590243/143‑1 (MQ=255)                                                                               tGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATc                                                         <  2:393108/143‑1 (MQ=255)                                                                                gTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTATGAGCGGCGTGTGCTGTGGCGTCTCGTGTTCAATTTCTGGTGTAATAACTGATTTCAGGGCATct                                                        >  1:99806/1‑142 (MQ=255)                                                                                      ttACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTATTCACGTCTTTCAGGGCATCGGTTCat                                                  >  2:4324/1‑143 (MQ=255)                                                                                       tACCCAGCTCGCAGCAGGCGAGCAAAGGACCAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAata                                                 <  2:219049/143‑1 (MQ=255)                                                                                          ccAGCTCGCAGCAGGCGAGCAAAGGCACAGCGCTGCGTAAGCAATAACCTGCGGTTGGCCCAGACGTCTGATCGGCGTTTCCCCCAACATGTTCTGTTCAATCTCTCGTGTACTAACGAATCTCAGCGCATCGGTTAATATTg                                              >  2:123200/1‑143 (MQ=255)                                                                                          ccAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTCATATTg                                              >  1:566430/1‑143 (MQ=255)                                                                                          ccAGCTCGCAGCAGGCAAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCAGTTAATATTg                                              >  1:87028/1‑143 (MQ=255)                                                                                                   agcagGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGgcg                                     >  1:441773/1‑143 (MQ=255)                                                                                                                        cgcTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGActat                >  1:382701/1‑143 (MQ=255)                                                                                                                         gcTGCGTTAGCAATATCCTGCGGTTAGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATAGGTTAATATTGGCCCCGGCGCAATGCCATTGACCCGaagact               >  2:68935/1‑139 (MQ=255)                                                                                                                                 aGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAACAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCAc       >  1:381059/1‑143 (MQ=255)                                                                                                                                      tatCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGTTGTTACATTTTTTCTTCAATTTCTGGTGTAATAACGCATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAgg  <  2:214773/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                           CAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGG  >  NC_000913/1697238‑1697513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑