Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,678,024 C→T intergenic (‑121/‑403) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,678,0240CT98.8% 260.8 / NA 84intergenic (‑121/‑403)pntA/ydgHpyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (42/41);  minor base A (1/0);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACG  >  NC_000913/1677885‑1678161
                                                                                                                                           |                                                                                                                                         
ttGGTATGCCAATTCGCATGGTATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTGAAATAAATTAGTAACAAACGCCCTATAATGTa                                                                                                                                        <  2:612811/143‑1 (MQ=255)
ttGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTa                                                                                                                                        <  1:452914/143‑1 (MQ=255)
 cggTATGCCAATTCGCATGATATCCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTAc                                                                                                                                       <  1:806533/142‑1 (MQ=255)
       gCCAATTCGCATGATATTCCCTTCCATCGGCATTATTGATGATTTTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGaaaa                                                                                                                                 >  1:800939/1‑143 (MQ=255)
        ccAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAAt                                                                                                                                <  2:303959/143‑1 (MQ=255)
           aTTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTAt                                                                                                                             <  2:269128/143‑1 (MQ=255)
            ttCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATg                                                                                                                            <  1:693895/143‑1 (MQ=255)
                cATGATATTCCCTTCCATCGGTCTTATTGATGATGGTTTGCCTGTGACAGGAGCCACACCAGCTGCTCATGTCCGCGCTAAATGTTACTCCGTAAAACGAAATCAGTAACAACCGCCCTCTAGTGTGCTGAAAATTATCCCtg                                                                                                                        >  2:489060/1‑143 (MQ=255)
                  tGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCtgt                                                                                                                       >  2:373459/1‑142 (MQ=255)
                  tGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCtgt                                                                                                                       <  1:373459/142‑1 (MQ=255)
                  tGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAAATATGCCtgtg                                                                                                                      >  2:1029066/1‑143 (MQ=255)
                    atatTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCGCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAAGAAACGCCCTATAATGTACTGAAAATTATGCCTGTGAt                                                                                                                    >  1:593775/1‑143 (MQ=255)
                       ttCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTa                                                                                                                 <  1:194947/143‑1 (MQ=255)
                       ttCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTa                                                                                                                 >  1:1115205/1‑143 (MQ=255)
                       ttCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTCTGATCTa                                                                                                                 >  2:63804/1‑143 (MQ=255)
                        tCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGAACTAg                                                                                                                >  1:566761/1‑143 (MQ=255)
                         cccTTCCGTCGGTTTTATTGATGATGGGTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAgc                                                                                                               <  2:847080/143‑1 (MQ=255)
                          ccTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAgcg                                                                                                              >  1:3116/1‑143 (MQ=255)
                            ttCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGcc                                                                                                            >  2:342926/1‑143 (MQ=255)
                               cATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCaaa                                                                                                         >  2:666262/1‑143 (MQ=255)
                               cATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCaaa                                                                                                         >  2:737175/1‑143 (MQ=255)
                                aTCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATCATGTACTGAAAATTATGCCTGTGATCTAGCGCCaaaa                                                                                                        >  1:759241/1‑143 (MQ=255)
                                aTCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATCATGTACTGAAAATTATGCCTGTGAACTAGCGCCaaac                                                                                                        >  2:971054/1‑142 (MQ=255)
                                aTCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCaaaa                                                                                                        >  1:979717/1‑143 (MQ=255)
                                aTCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCaaaa                                                                                                        >  2:399522/1‑143 (MQ=255)
                                  ctctgctATTTATGACGGTTGGCCTGTGTCCTGAGCCACACAACCGCCGCATGTACGAGCTAAATGTTACTCCGCTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAAt                                                                                                      <  2:187809/137‑1 (MQ=255)
                                   ggTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATACTGTACTGAAAATTATGCCTGTGATCGAGCGCCAAAAATc                                                                                                     >  1:224224/1‑143 (MQ=255)
                                      tttATTGGTGATGGTGTGCGTGTGCCAGGAGCCCCGCAAGCTGCTCATGTGCGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGt                                                                                                  <  2:223160/143‑1 (MQ=255)
                                       ttATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAACTTATGCCTGTGATCTAGCGCCAAAAATCAGTa                                                                                                 >  1:243308/1‑143 (MQ=255)
                                              tgatgGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGc                                                                                          <  1:708546/143‑1 (MQ=255)
                                              tgatgGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGc                                                                                          <  1:183742/143‑1 (MQ=255)
                                                atgGTTTGCGTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGt                                                                                        <  1:66178/143‑1 (MQ=255)
                                                 tgGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGtgtg                                                                                       >  1:170739/1‑143 (MQ=255)
                                                        ccTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGc                                                                                <  1:529458/143‑1 (MQ=255)
                                                        ccTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGc                                                                                <  1:768585/143‑1 (MQ=255)
                                                         cTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGACAATAATGCCTGTGATCTAGCGCCAAAAATCCGTATTTCGGCCTGAAGCCGCa                                                                               >  2:125082/1‑143 (MQ=255)
                                                         cTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGCTCTCGCGCCAACAATCAGTATTTCGTCGTGAAGTCGCa                                                                               >  2:230745/1‑143 (MQ=255)
                                                         cTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCa                                                                               >  1:223923/1‑143 (MQ=255)
                                                             gtCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAat                                                                           >  1:1049889/1‑143 (MQ=255)
                                                              tCAGGAGCCACGCAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTCTAATGTACTGCAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAata                                                                          >  1:888120/1‑143 (MQ=255)
                                                                  gAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTaa                                                                      <  1:20508/143‑1 (MQ=255)
                                                                  gAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTaa                                                                      >  1:301829/1‑143 (MQ=255)
                                                                   aGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAc                                                                     <  1:1056800/143‑1 (MQ=255)
                                                                        cacaAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAACAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCGAAATATTAACGATTc                                                                >  1:826245/1‑143 (MQ=255)
                                                                                gctCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGa                                                        <  1:273924/143‑1 (MQ=255)
                                                                                  tCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATa                                                      <  2:800939/143‑1 (MQ=255)
                                                                                    aTGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTAAACTCGCAAAATATTAACGATTCAGCCATGATAGt                                                    >  2:603895/1‑143 (MQ=255)
                                                                                      gTACGAGCTAAATGTTACTCTGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTCGCGCCTTAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTgg                                                  <  2:1134593/143‑1 (MQ=255)
                                                                                      gTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTgg                                                  <  2:112032/143‑1 (MQ=255)
                                                                                         cGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGAt                                               <  1:708721/143‑1 (MQ=255)
                                                                                           aGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATaa                                             <  1:323037/143‑1 (MQ=255)
                                                                                           aGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGACCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAAACAGTATTTCGGCGTGAACTCGCAAAATATAAAAGAATCAGCCGTCATAGTGGGATaa                                             >  2:563648/1‑143 (MQ=255)
                                                                                               aaaTGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAACTATTAACGATTCAGCCGTGATAGTGGGATAAacac                                         >  2:790752/1‑143 (MQ=255)
                                                                                                  tGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCtt                                      >  1:665446/1‑143 (MQ=255)
                                                                                                   gTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTa                                     <  1:778446/143‑1 (MQ=255)
                                                                                                      aCTCCGTTAAAATAAATTAGTACCAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGaa                                  <  2:557321/143‑1 (MQ=255)
                                                                                                      aCTCCGTTAAAATAAATTAGTAACTAACGCCCTATAATGTACTGAAAATTATGTCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGaa                                  <  2:557346/143‑1 (MQ=255)
                                                                                                      aCTCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGaa                                  <  2:50497/143‑1 (MQ=255)
                                                                                                        tCCGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCCGCCGTGATAGTGCGATAAGCACCttgcgacgc                               >  2:801486/1‑136 (MQ=255)
                                                                                                          ctttAAAATATATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGcc                              <  2:379890/141‑1 (MQ=255)
                                                                                                          ccttAAAATAAATTAGTAACAACCGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGcc                              <  1:133766/141‑1 (MQ=255)
                                                                                                          cGTTAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGcc                              <  2:979717/143‑1 (MQ=255)
                                                                                                          cGTTAAAATAAATTAGTAACAAACGATATCTAAAGAACGGAAAAATATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGcc                              >  2:85976/1‑143 (MQ=255)
                                                                                                            ttAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAAGAATCAGCCGTGACAGTGGGATAAACACCTTACAACGCCgg                            >  2:493090/1‑143 (MQ=255)
                                                                                                            ttAAAATAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCAAAAAATCAGTATTTCGGCGTGAACTCGCAACATATTAACGATTCAGCCGGGATAGTGGGATAAACAGCTTAGCACGCCgg                            >  2:1092904/1‑143 (MQ=255)
                                                                                                              aaaaTAAATTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGAt                          <  1:674621/143‑1 (MQ=255)
                                                                                                               aaataaatTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGGCAAATAGCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATa                         >  1:795094/1‑143 (MQ=255)
                                                                                                                 ataaatTAGTAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATaaa                       <  1:342926/143‑1 (MQ=255)
                                                                                                                          tAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATaa              >  1:418534/1‑143 (MQ=255)
                                                                                                                          tAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATaa              >  2:177330/1‑143 (MQ=255)
                                                                                                                          tAACAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATaa              <  2:1049889/143‑1 (MQ=255)
                                                                                                                            aCAAACGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATCTTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCCGATAAAGACTGATCAtt            >  2:1029557/1‑143 (MQ=255)
                                                                                                                              aaaCGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAACGACTGATAATTGt          >  1:578300/1‑143 (MQ=255)
                                                                                                                               aaCGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGCTAAAGACTGATAATTATc         >  2:759399/1‑143 (MQ=255)
                                                                                                                               aaCGCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTc         <  2:223923/143‑1 (MQ=255)
                                                                                                                                  gCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGTGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTc      <  2:170739/143‑1 (MQ=255)
                                                                                                                                  gCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTc      <  2:602519/143‑1 (MQ=255)
                                                                                                                                  gCCCTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTc      <  1:737175/143‑1 (MQ=255)
                                                                                                                                   cacTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATAAGTATTTCGGCGTAAACTCGCAAAATATTAACGATTCAGCCGTGAAAGTAGGATAAAAACATTAGAACGCCGGATCAAGACTGGTAATTGtactgg     >  2:187543/3‑138 (MQ=255)
                                                                                                                                    ccTATAGTGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGa    <  1:125082/143‑1 (MQ=255)
                                                                                                                                    ccTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGa    >  2:810934/1‑143 (MQ=255)
                                                                                                                                     cgataATGTACTGAAAACTAAGCCCCTAATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGAc   <  1:210649/141‑1 (MQ=255)
                                                                                                                                     cTATAATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGAc   <  1:418913/143‑1 (MQ=255)
                                                                                                                                      tataATGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGAGAAACTGGTATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACg  >  1:1152786/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACG  >  NC_000913/1677885‑1678161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: