New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3220246	148 (1.130)	5 (0.040)	5/484	NT	3.2%	coding (753/1380 nt)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
	?	NC_000913	= 3220398	162 (1.290)					coding (905/1380 nt)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Rejected: Frequency below/above cutoff threshold.

TCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3219950‑3220246 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccgccgccgAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGGTTTTTTTGCACTCGTTAAGAGCCGTGCGCATGGCTTCGATATTGCCAAACGGCACAT  <  NC_000913/3220398‑3220119                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   TCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGAAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTG                                                                                                                                                                                                                                                                                                   <  2:1029505/288‑1  CCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGC                                                                                                                                                                                                                                                                                                  <  1:779300/288‑1       GAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGC                                                                                                                                                                                                                                                                                            <  1:256059/289‑1                                                                                                                     GGCTAAATCGCCCTGCCGCAATCCTTTTATGCCGTTACTGCCGGGCTTCCGTCATTTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGT                                                                                                                                                                              <  2:331868/289‑1                                                                                                                     GGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGT                                                                                                                                                                              >  1:331868/1‑289                                                                                                                                                                                                                                                     CGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTAATCGAGGATCATCAGTGCGCCGACCTCATCGCATAGCTTACGTACGCCGGTGAGCTAGCCCGGCGGCGCGCGAATTCCGTCACCTTCACCCTGCATGGGTGCGAGGATCACCGCAGCGCCAACACCCACGGTCTTTTTcccct                                               >  2:258688/1‑283                                                                                                                                                                                                                                                                                    atccgcaagcagcCCGCCGCCGGGCGCATTGGCAAGTCAGAGGATATCCGGGTGTACGTTCTCATGCTCGCAGGCGAAGATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCGTCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGGTTTTTGTGCACTCGTTAAGAGCCGTGCGCATAGATTCGATATTG               <  2:322589/276‑1                                                                                                                                                                                                                                                                                      GCGTAATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGGTTTTTTTGCACTCGTTAAGAGCCGTGCGCATGGCTTCGATATTGCC             >  1:19255/1‑289                                                                                                                                                                                                                                                                                          AATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCCGTTTTTTTTCACTCGTTACGACCCGTTCCAATGCCTCCCATATCGCCAAA          >  2:1201543/1‑288                                                                                                                                                                                                                                                                                                 CCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGGTTTTTTTGCACTCGTTAAGAGCCGTGCGCATGGCTTCGATATTGCCAAACGGCACAT  <  1:702472/289‑1                                                                                                                                                                                                                                                                                                    CCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGG                                                           >  1:28204/1‑229                                                                                                                                                                                                                                                                                                    CCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGG                                                           <  2:28204/229‑1                                                                                                                                                                                                                                                                                                    CCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACC                                                                              >  1:247972/1‑210                                                                                                                                                                                                                                                                                                    CCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACC                                                                              <  2:247972/210‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   TCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3219950‑3220246 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccgccgccgAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGGTTTTTTTGCACTCGTTAAGAGCCGTGCGCATGGCTTCGATATTGCCAAACGGCACAT  <  NC_000913/3220398‑3220119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.