Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,922 IS5 (–) +4 bp noncoding (24‑27/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3652036 =NA (NA)77 (1.250) 55/284 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4049925 0 (0.000)noncoding (27/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 = 3653230NA (NA)74 (1.200) 61/284 0.0 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4049922 = 0 (0.000)noncoding (24/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

Only 100 of 148 total aligned reads displayed.

AAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGT  >  NC_000913/4049780‑4050064
                                                                                                                                              |                                                                                                                                              
aagtaagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTc                                                                                                                                                >  1:754233/1‑143 (MQ=255)
aagaaaatctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccc                                                                                                                                                >  2:632314‑M2/143‑143 (MQ=255)
  gaaaatctcttttctggtctgacggcgcttactgctgaattcactgtctgcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTa                                                                                                                                              >  2:1041083‑M2/141‑143 (MQ=255)
  gaaaatctcttttctggtctgacggcgcttactgctgaattcactgtcggctaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcaggaacgtgttcgcgccttccCTa                                                                                                                                              >  2:593360‑M2/141‑143 (MQ=255)
    aaGAAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATg                                                                                                                                            >  2:163327‑M2/1‑142 (MQ=255)
     aatctcttttctggtctgacgacgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatcatgaaccctgttctatggctccagatgacaaacctgatctcaaagcagggaattgttcgcaccttccctaaat                                                                                                                                           >  2:162001‑M2/138‑140 (MQ=255)
      atctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCCATCtt                                                                                                                                          >  2:610262‑M2/137‑143 (MQ=255)
       tctcttttctgctctgacggcgcttactgctgaattcactgtcggcgtagttaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCttt                                                                                                                                         <  1:153129‑M2/8‑1 (MQ=255)
        ctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTc                                                                                                                                        <  1:102677‑M2/9‑1 (MQ=255)
         tcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCa                                                                                                                                       <  1:371703‑M2/10‑1 (MQ=255)
           aTAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtg                                                                                                                                     <  2:903596‑M2/143‑9 (MQ=255)
            tAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgc                                                                                                                                    >  2:213022‑M2/1‑134 (MQ=255)
            tAAGCTGTCACGTGTAGGGATGGCTAGTAGAAATCCCTATGCAACAACTGAAAAAAAATTACAAAAACTGCGTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgc                                                                                                                                    >  1:49156‑M2/1‑134 (MQ=255)
                tggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCttt                                                                                                                                <  1:919214‑M2/17‑1 (MQ=255)
                tggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCttt                                                                                                                                <  1:259368‑M2/17‑1 (MQ=255)
                  gtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatcgcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTa                                                                                                                              <  1:1035338‑M2/19‑1 (MQ=255)
                   tctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTAc                                                                                                                             <  1:1115070‑M2/20‑1 (MQ=255)
                     aCGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGCGTACAGAGGTAAGATGATCTATggaaggtgcgaatcaacg                                                                                                                           <  2:124315‑M2/143‑19 (MQ=255)
                      cGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcgg                                                                                                                          >  2:754233‑M2/1‑124 (MQ=255)
                      agtTTTGTGATGGCTATTAGAAATTCCTATGCAACAACGGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcgg                                                                                                                          >  2:478499‑M2/2‑124 (MQ=255)
                               tactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCg                                                                                                                 >  2:359780‑M2/112‑143 (MQ=255)
                                tGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattctt                                                                                                                >  1:333284‑M2/1‑114 (MQ=255)
                                  gCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttct                                                                                                              <  2:36797‑M2/143‑32 (MQ=255)
                                    tATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAAAGGAAAGCTAGGCACGTAAGGTACAAGGGTAAGATGTTGTATggaaagtccgaataagcgggcacattcttctcg                                                                                                            <  2:284586‑M2/143‑34 (MQ=255)
                                    ctgaattcactgtcggcgaaggtaagttgatgactcatgatgtgtcctgttctatgactccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGAttt                                                                                                            <  1:208331‑M2/37‑1 (MQ=255)
                                    ctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGGAATCGGAttt                                                                                                            >  1:641817‑M2/107‑143 (MQ=255)
                                      ttAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGCAAGATGTCCTATggaaggtgcgaataagcggggaaattcttctcggc                                                                                                          <  2:24444‑M2/143‑36 (MQ=255)
                                       tAGAAATGCCCATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAAGTGAACGAAAAGGAGTAAAGTTAGGCGCGTAGGGTACAGAGGGAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggct                                                                                                         >  2:187237‑M2/1‑107 (MQ=255)
                                        aGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGAAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctg                                                                                                        <  2:617327‑M2/143‑38 (MQ=255)
                                        aGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACGGAACAAAAAAGAGTAAAGGTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttgtcggcgg                                                                                                        <  1:185012‑M2/143‑38 (MQ=255)
                                           aaTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgact                                                                                                     >  1:1017269‑M2/1‑103 (MQ=255)
                                                 tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtca                                                                                               <  1:219637‑M2/143‑47 (MQ=255)
                                                 cggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATAtttt                                                                                               <  1:244384‑M2/50‑1 (MQ=255)
                                                  ggcggagggaaggtgatgactcgtgatgaactctgttctatggctgcagatgccaaacatgttctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTa                                                                                              <  2:20532‑M2/51‑1 (MQ=255)
                                                   tGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatt                                                                                             >  1:892831‑M2/1‑95 (MQ=255)
                                                   gcgaaggttagttgttgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                                                             <  2:719232‑M2/52‑1 (MQ=255)
                                                   gcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                                                             >  1:1097406‑M2/92‑143 (MQ=255)
                                                   gcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCGGATgattttca                                                                                             >  2:1017397‑M2/92‑141 (MQ=255)
                                                    cgaaggtaagttgatgactcatgatgcaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgc                                                                                            <  2:727269‑M2/53‑1 (MQ=255)
                                                            tGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttctt                                                                                    <  1:480975‑M2/143‑58 (MQ=255)
                                                            tGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcattccatttctt                                                                                    <  2:8859‑M2/143‑58 (MQ=255)
                                                              aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAAAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcctttcttca                                                                                  <  1:630180‑M2/143‑60 (MQ=255)
                                                                  tgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCccagtcagtc                                                                              >  1:655415‑M2/77‑142 (MQ=255)
                                                                  tgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTa                                                                              <  1:732778‑M2/67‑1 (MQ=255)
                                                                   aaaTTACAGAGAGTGCAGTATGAGCGGAACAAAAGAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgttt                                                                             >  2:377562‑M2/1‑79 (MQ=255)
                                                                   aaaTTACAAAAAGTGCTGTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgttt                                                                             >  2:281462‑M2/1‑79 (MQ=255)
                                                                    actcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAt                                                                            <  1:69010‑M2/69‑1 (MQ=255)
                                                                      tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGCCTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGa                                                                          >  2:587999‑M2/73‑143 (MQ=255)
                                                                      tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGa                                                                          >  1:1034614‑M2/73‑143 (MQ=255)
                                                                        atgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGACTATTTTAGCCGCCCCAGTCAGTAATGACt                                                                        >  1:264104‑M2/71‑143 (MQ=255)
                                                                         cAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatggttgagccg                                                                       <  2:961127‑M2/143‑71 (MQ=255)
                                                                          gatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTgg                                                                      <  1:126760‑M2/75‑1 (MQ=255)
                                                                          aaaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAATTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccga                                                                      <  1:721235‑M2/143‑72 (MQ=255)
                                                                             gaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGc                                                                   >  2:434489‑M2/66‑143 (MQ=255)
                                                                              aGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttt                                                                  >  1:173026‑M2/1‑68 (MQ=255)
                                                                                tGCTTTCTGAACTGAACAAAAAAGAGTAAAGGTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttttt                                                                >  2:731872‑M2/1‑66 (MQ=255)
                                                                                ccctgttctatggctccaggcgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGgcggc                                                                <  1:295877‑M2/81‑3 (MQ=255)
                                                                                 gcagtctaaACGGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttc                                                               >  2:958465‑M2/5‑65 (MQ=255)
                                                                                 cctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGttt                                                               >  1:154428‑M2/62‑143 (MQ=255)
                                                                                  ctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATATTGCAGACCTCTTACTTCACGTAATCGGATTTGGCGGAATATCTTAGCCGCCTCAGTCAGCAATGAACGgggcgcgtc                                                              >  2:277288‑M2/61‑139 (MQ=255)
                                                                                   tttCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctc                                                             >  2:107206‑M2/1‑63 (MQ=255)
                                                                                   tttCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctc                                                             <  2:735038‑M2/143‑81 (MQ=255)
                                                                                   tgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgccccttccCTATCGTTCAGACCTTTTACTTCACGTAATCGCATCTGGCTGAATACTTTAGCCGCCCCAGTCCGTAAGTCCTGGggagtagct                                                             >  2:390827‑M2/60‑139 (MQ=255)
                                                                                      ctgaactgaaCAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccg                                                          >  1:235405‑M2/1‑60 (MQ=255)
                                                                                      ctgaacggaaCAAAAAGGAGTAAAGATGGTGGCGGGGGGGAGAGGGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccg                                                          >  2:1112170‑M2/1‑60 (MQ=255)
                                                                                         atggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTCCAGACTTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTgg                                                       <  1:1113665‑M2/90‑1 (MQ=255)
                                                                                         aactgaacAAAAAAGGGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggcaggtgcgaataagcggggaaattcttctcggccgactcagtcatataatttcttcatgtttgagccgattttttcttctgtaa                                                       <  1:342589‑M2/143‑87 (MQ=255)
                                                                                            gctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCg                                                    <  1:320073‑M2/93‑1 (MQ=255)
                                                                                             ctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTGATTGGGCga                                                   >  1:557546‑M2/50‑143 (MQ=255)
                                                                                              tccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGGTTTTTATTGGGcgag                                                  >  1:448632‑M2/49‑142 (MQ=255)
                                                                                               acAAAAGATGGCAAGGTTAGTCCGGTAGGGTACAGCGGTAAGATGTCCTATggaaggtgcgaataagcggggaaattcttctcggctgcctcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgcct                                                 >  1:783611‑M2/1‑51 (MQ=255)
                                                                                               acAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggttcggataggcggggacattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgcct                                                 >  2:389719‑M2/1‑51 (MQ=255)
                                                                                                cAAAAAAGAGTAAAGTTAGTCGCGGAGTGTACAGAGGTAAGATGTCCTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttcagcagctttttgctcccgtaaatgcctt                                                >  2:681718‑M2/1‑50 (MQ=255)
                                                                                                  gatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGCATATTTTCGCCGTCGCAGTCAGTAATGACTGGGGCGTTCTTTATTGGGCgaaagaa                                              >  2:123906‑M2/45‑143 (MQ=255)
                                                                                                   atgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaagaaa                                             <  1:849066‑M2/100‑1 (MQ=255)
                                                                                                   atgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaagaaa                                             <  1:562274‑M2/100‑1 (MQ=255)
                                                                                                   aaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggtgatgcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgta                                             <  1:1003702‑M2/143‑97 (MQ=255)
                                                                                                    tgacaaacatgatctcatatcagggacttgttcgcacctttcCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                                            <  1:106583‑M2/101‑1 (MQ=255)
                                                                                                    tgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                                            <  1:959902‑M2/101‑1 (MQ=255)
                                                                                                     gacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaag                                           >  1:971962‑M2/42‑143 (MQ=255)
                                                                                                      agagTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcgtgtttgcgccgattttttctcacgtaaatgccttgcagca                                          <  2:293402‑M2/143‑100 (MQ=255)
                                                                                                      acaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGGTTTTTATCGGGCGaaagaaaaga                                          >  2:1081388‑M2/41‑143 (MQ=255)
                                                                                                        aaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATc                                        >  1:131181‑M2/39‑143 (MQ=255)
                                                                                                          acatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTACCCGCACTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGAGCGAAAGAAAAGATCCg                                      >  2:445620‑M2/37‑143 (MQ=255)
                                                                                                              gtggggcgcgtagggtACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctattt                                  >  1:499705‑M2/7‑36 (MQ=255)
                                                                                                               acctcatatcaggggcttgttcgcaccttctCTATCTTTCAGACCTTGTACTTCACGTAATCGGATTTGGCTGAAGATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATg                                 <  2:171604‑M2/112‑1 (MQ=255)
                                                                                                                 ctcatatcagggactggttcgcaccttccCTATCTTTCAGACAATTAACGATGCTTATGTGTATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGcc                               <  1:813022‑M2/114‑1 (MQ=255)
                                                                                                                 aGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttaga                               >  1:710291‑M2/1‑33 (MQ=255)
                                                                                                                 aGGCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttaga                               >  1:636306‑M2/1‑33 (MQ=255)
                                                                                                                  ggcgcgtgGGGTACAGGGGTAGGCGGGTCTATggaaggtgctaatacgcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagac                              >  2:1027421‑M2/3‑32 (MQ=255)
                                                                                                                      atcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATg                          >  1:501385‑M2/25‑143 (MQ=255)
                                                                                                                         cgggacttgttcgcaccttccGTATCTTTGAGACCTTTTACTTGACGTAATCGGATTTGGCTGAATATTTTAGCTGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATgcgc                       <  2:822010‑M2/122‑1 (MQ=255)
                                                                                                                           ggTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttc                     >  1:922748‑M2/1‑23 (MQ=255)
                                                                                                                            gTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttttgcccccgtaaatgccttgaatcagcctatttagaccgtttcttcg                    >  2:79951‑M2/1‑22 (MQ=255)
                                                                                                                               ttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTCTTTATTGGGCGAAAGAAAAGATCCGTACTACCTGCTGCGCTATGtt                 >  1:403039‑M2/16‑143 (MQ=255)
                                                                                                                                tgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGttt                <  1:36925‑M2/129‑1 (MQ=255)
                                                                                                                                      caccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAgg          >  1:590385‑M2/9‑143 (MQ=255)
                                                                                                                                       cgtactgctatggatggtgcgattcatcgtggaaattcttctcggctgactcagtcctttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggc         >  2:30929‑M2/8‑11 (MQ=255)
                                                                                                                                           gTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgtta     <  1:612950‑M2/143‑137 (MQ=255)
                                                                                                                                              cTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGt  <  1:367989/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
AAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGT  >  NC_000913/4049780‑4050064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.