| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,415,807 | A→C | D354A (GAT→GCT) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,415,807 | 0 | A | C | 98.2% | 159.9 / NA | 55 | D354A (GAT→GCT) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (33/20); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAC > NC_000913/2415667‑2415941 | cGGTTACGAAATGGACGCGCGCATTTCTAAACTATGAGAACGCACTGTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTGCGGTTGACGCTc > 1:767276/1‑143 (MQ=255) ggTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCa > 1:578783/1‑143 (MQ=255) gTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCAc > 2:825005/1‑143 (MQ=255) tACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCCACCTGGAAGTTCCGGTTGACGCTCACgc > 1:335134/1‑142 (MQ=255) tGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATc > 1:794360/1‑143 (MQ=255) aCGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGCTCCGGTTGACGCTCACGAACGTATCcag > 2:617559/1‑143 (MQ=255) gcgcgcATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACAAACAACTGGCAGACCTCTCTGAGCCTGCAGAGCGTCAACATGGAAGTTGCGGTTGACGATCGCGAACGGATcacggc > 2:894820/1‑138 (MQ=255) cgcgcgTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTAAGTATTGTGTACTCCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa < 2:127586/143‑1 (MQ=255) cgcgcATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa < 1:328680/143‑1 (MQ=255) cgcgcATTGCTAAACTGTGCGAACGTGCTTTCGCTGCCGGCCTGCCGGTATTTATGGTGAACACCAACACCGGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa < 1:994671/143‑1 (MQ=255) ctttCTAAAGTGTGCGCACGTGCTTTCGCTACCGGACTGCCGGTCTTTGTGGTGAACCCCACCACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTACACCAGTGCCAAGCAATTGACGCTCACGAACGTATCGAGAAAGTTCa < 2:676013/142‑1 (MQ=255) aTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGACCGTATCGAGAAAGTTCa > 1:1015224/1‑143 (MQ=255) aTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCa < 1:574431/143‑1 (MQ=255) ttacTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAg < 1:294698/140‑1 (MQ=255) ttCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCTGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGCTCAgg > 2:820526/1‑143 (MQ=255) tAAACTGTGCGAACGTGCTTTCGCTACCGACCTGCCGGTATGTGTTGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAAt < 1:1056236/143‑1 (MQ=255) tgCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCCACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGACCGTTCAGGAATACggcgc > 2:406949/1‑139 (MQ=255) gCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGACCGTATCGAGAAAGTGCAGGAATACGTTGCt > 1:1015683/1‑143 (MQ=255) gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGACGCTCCTGTTGACGCTCACGACCGTATCGAGAAAGTTCAGGAATACGTTGATaa > 2:145634/1‑143 (MQ=255) gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:786796/1‑143 (MQ=255) gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTACCGGTTGACGCTCACGAACGTATCGAGAAAGTTGAGGAATACGGTGCTaa > 2:557654/1‑143 (MQ=255) gCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCACTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCACACGAACGTATCGAGAAAGATCAGGAATACGCTACTAACTACAACAACGc > 1:11351/1‑143 (MQ=255) tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTg > 1:578560/1‑143 (MQ=255) cTGCCGGTGGTTGTGGTGACCACGCCCACCTGGCAGACCTCTCTGAGCCGGTAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt < 2:705394/143‑1 (MQ=255) tGCCTGTATTTATGGTGACCACCAACACCTGGCAGACCTCTGTGAGCCTGCAGAGCTTCAACCTGGAATTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATc < 2:86635/143‑1 (MQ=255) ccGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGa > 1:888151/1‑143 (MQ=255) cGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGAGTGGATCgga > 1:237434/1‑141 (MQ=255) gTATTTATGGTGACCACCAACACCTGCCAGCCCTCGCTTAGCCTGCATGGCTTCAACCTTGAAGTTCCCGTTGACGCTCACTAACGTATCGAGATAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTCGATCGAAtc < 2:875897/143‑1 (MQ=255) tttATGGTGACCACCAACACCTGGCAGATCTCTCTGAGCCTGCAGAGCTTCAACCTGGCAGCTCCGGTTGACGTTCACGTACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATctct < 1:238853/143‑1 (MQ=255) gacggTGAACGCCAACGCCTGGCAGACCTCGCTCAGCCTGCAGAGCTTCAACCTGGAAGGTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGa < 1:62783/140‑1 (MQ=255) aTGGTGAACACCAACCCCTGGCAGCCCTCTCTGGGCCTGCAGAGCGTCGACCGGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAc < 2:969346/143‑1 (MQ=255) cacaccaacgccGGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTTCATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:722369/142‑1 (MQ=255) aacaccaacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTACCTACCTCCACGCTGCCTGGATCGAATCGCTGACTGCCAc > 2:346485/1‑143 (MQ=255) caccaacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGCAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACACCAACGCTGACTGGATCGAAGCTCTGACTGCCACtt > 2:955069/1‑143 (MQ=255) caacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTg > 1:730710/1‑143 (MQ=255) cTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCa > 1:584068/1‑143 (MQ=255) tGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGGTCCGGTTGACACTCACGAACGTATCGAGCAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGAGCAg > 1:736095/1‑143 (MQ=255) aCCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcg > 1:188455/1‑143 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAGTGCCACTTCTGGGCGCCGCCgccgtc > 1:863269/1‑143 (MQ=255) ctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtc > 1:296122/1‑143 (MQ=255) ctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGACACTTCTGAGCGCAGCCGTCgtctgtc > 1:296458/1‑143 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgt < 2:41823/127‑1 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgt > 1:41823/1‑127 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTg > 1:847796/1‑143 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTg > 1:708411/1‑143 (MQ=255) agagCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAGCGTAGCGAGAAAGTTCAGGAATACGTTGCTCACCACGTCAACGCTGACTGGATCGCAGCTCTGACTGCCACTGCGGAGCGCGGCCGTCGCCCGTCCCCGCCcgcg > 2:909513/1‑143 (MQ=255) cTTTAACCTGGAAGTTCCGGTTGACGCACACGACCGTATCGAGAAAGGTCAGGAAGACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTcc < 1:1036912/143‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACCTCAACGCTGACTGGATCGAATCTCTGCCTGCCCCTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTcc > 1:662869/1‑143 (MQ=255) aaCCTGGAAGTTCCGGTTGACGCTCACGCACGTATCGAGACAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTa < 1:474943/143‑1 (MQ=255) cTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCa > 2:816573/1‑143 (MQ=255) gAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTCTCAGct > 1:1050975/1‑143 (MQ=255) gAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGct < 1:956485/143‑1 (MQ=255) gtgccGGTTGACGCGCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACGGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGctgac < 1:145634/140‑1 (MQ=255) cGGTTGACGCTCACGACCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAGCGAATCTCTGCCGGCCACCTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGaa < 1:67936/143‑1 (MQ=255) ggTGGACGCTCACGAACGTACCGAGAAAGTTCAGGAATGCGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTCACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAc < 1:581488/143‑1 (MQ=255) | CGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAC > NC_000913/2415667‑2415941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |