Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,922 IS5 (–) +4 bp noncoding (24‑27/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3652036 =NA (NA)66 (1.290) 53/284 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4049925 0 (0.000)noncoding (27/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 = 3653230NA (NA)59 (1.160) 50/284 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4049922 = 0 (0.000)noncoding (24/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

Only 100 of 129 total aligned reads displayed.

AGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAAC  >  NC_000913/4049781‑4050061
                                                                                                                                              |                                                                                                                                           
agtaagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTATGAACTGAACAAAAAAGAGTAAAGTTAGACGCGTAGGGTACAGAGATAAGGTGTTCt                                                                                                                                            >  2:360781/1‑143 (MQ=255)
 gtaagCAACATGAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTa                                                                                                                                           >  2:128121/1‑143 (MQ=255)
  taagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAGGATGTTCTAt                                                                                                                                          >  1:427285/1‑143 (MQ=255)
  taagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTAt                                                                                                                                          <  1:448125/143‑1 (MQ=255)
     aatctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagctgacaaacaggatctcatatcagggacttgttcgcaccttccCTATCt                                                                                                                                        >  1:170532‑M2/138‑143 (MQ=255)
      cAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaa                                                                                                                                      <  1:493807‑M2/143‑5 (MQ=255)
         cATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggt                                                                                                                                   <  1:552953‑M2/143‑8 (MQ=255)
          aTAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGCGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtg                                                                                                                                  <  2:270957‑M2/143‑9 (MQ=255)
            tgtcgggtctgagggcgcttacgtctgaattcgctgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGAc                                                                                                                                 <  2:855040‑M2/13‑1 (MQ=255)
            aaGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTCGGGTACAGAGGTACGATGTTCTATggagggtgcg                                                                                                                                <  1:26746‑M2/143‑11 (MQ=255)
              gCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGATCAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaa                                                                                                                              <  1:907132‑M2/143‑13 (MQ=255)
               cTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAGAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaat                                                                                                                             <  2:450762‑M2/143‑14 (MQ=255)
                tggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactgatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCttt                                                                                                                             >  1:419107‑M2/127‑143 (MQ=255)
                     tgacgtcgcttactgctgaattcactgtcggcgcaggtaagttgatgactcatgatgaaccgtgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACtt                                                                                                                        <  2:31181‑M2/22‑1 (MQ=255)
                      tttttgTAATGTCTAGTAGAAAATCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAATAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAATATGTTCTATggaaggtgcgaataagcggg                                                                                                                      >  2:36305‑M2/2‑123 (MQ=255)
                      gTTGTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggg                                                                                                                      >  2:317763‑M2/1‑123 (MQ=255)
                        cggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCAc                                                                                                                     <  1:359130‑M2/25‑1 (MQ=255)
                            tgATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaatt                                                                                                                >  2:780393‑M2/1‑117 (MQ=255)
                            tgATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaagtgcgaataatcggggaaatt                                                                                                                <  1:692800‑M2/143‑27 (MQ=255)
                             gATGGCTATTATAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattc                                                                                                               >  2:619946‑M2/1‑116 (MQ=255)
                              ttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATc                                                                                                               >  2:494406‑M2/113‑143 (MQ=255)
                                 gCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcgggaaattcttctc                                                                                                           <  1:702182‑M2/143‑32 (MQ=255)
                                    ctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGAttt                                                                                                         >  1:697172‑M2/107‑143 (MQ=255)
                                     ttAGCAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggc                                                                                                       >  2:214216‑M2/1‑108 (MQ=255)
                                     tgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTACTCGAATTTg                                                                                                        >  2:865221‑M2/106‑143 (MQ=255)
                                     tgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTg                                                                                                        <  2:210548‑M2/38‑1 (MQ=255)
                                      gaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttcgatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTgg                                                                                                       <  2:84456‑M2/39‑1 (MQ=255)
                                       aGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAAATGTTATATgcaagattctaataagcggggaaatacttctcggctg                                                                                                     <  2:225667‑M2/143‑38 (MQ=255)
                                        gAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctga                                                                                                    <  1:877354‑M2/143‑39 (MQ=255)
                                        attcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCt                                                                                                     <  1:685912‑M2/41‑1 (MQ=255)
                                        attcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCt                                                                                                     >  1:886294‑M2/103‑143 (MQ=255)
                                             tCCTACGCAGCAAAGAAAAAAAAATTAAAAAAAGGGCTTTCGGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcag                                                                                               >  2:816997‑M2/1‑100 (MQ=255)
                                                tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtca                                                                                            <  1:711661‑M2/143‑47 (MQ=255)
                                                tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtca                                                                                            <  1:519386‑M2/143‑47 (MQ=255)
                                                 aTGCCACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCGATggaaggtgcgagtaagcgggggaagtgttctcggctgagtcggtcat                                                                                           <  2:527396‑M2/143‑48 (MQ=255)
                                                 aTGCAACAACTGAAAAAAAATTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAAAGAAAAGTAAGTCGGGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaactccttctcggctgactaggtcc                                                                                            <  2:328385‑M2/143‑47 (MQ=255)
                                                 aTGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcat                                                                                           <  2:526021‑M2/143‑48 (MQ=255)
                                                  ggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTa                                                                                           <  2:529732‑M2/51‑1 (MQ=255)
                                                  ggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTa                                                                                           <  2:746960‑M2/51‑1 (MQ=255)
                                                  ggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTGTACTTAACGGAATCTCATTTGGCTGCCTATGTTa                                                                                           >  2:287280‑M2/93‑143 (MQ=255)
                                                   gcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                                                          <  2:817113‑M2/52‑1 (MQ=255)
                                                   gcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttcaCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                                                          >  2:516844‑M2/92‑143 (MQ=255)
                                                       caacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatt                                                                                     >  1:402541‑M2/1‑90 (MQ=255)
                                                        aacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcattt                                                                                    <  1:467781‑M2/143‑55 (MQ=255)
                                                        aacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcattt                                                                                    <  1:243987‑M2/143‑55 (MQ=255)
                                                           aagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGACTATTTTAGCCGCCCca                                                                                  >  2:344148‑M2/84‑143 (MQ=255)
                                                             aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttca                                                                               <  1:837538‑M2/143‑60 (MQ=255)
                                                               aaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgcctcagtgagtgcatttcttcatg                                                                             <  2:475845‑M2/143‑62 (MQ=255)
                                                                   gactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTaa                                                                          >  1:884972‑M2/76‑143 (MQ=255)
                                                                   gactcatgatgaaccctggtctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTaa                                                                          <  2:466936‑M2/68‑1 (MQ=255)
                                                                    actcatgatgaaccctgttctatggctccacgtaacaattattatctcatatcagtgacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAt                                                                         <  2:883864‑M2/69‑1 (MQ=255)
                                                                    aTTACAAAAAGTGCTTTCTGAACTGAACAAACAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttga                                                                        >  1:88190‑M2/1‑77 (MQ=255)
                                                                    aTTAAGAACAGTGCTTGCTGAACGGGACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttga                                                                        >  2:715214‑M2/1‑77 (MQ=255)
                                                                     ttACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgag                                                                       >  1:235699‑M2/1‑76 (MQ=255)
                                                                      tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatctgggacttattcgcaccttccCTATCTTTCAGACCTCTTACTTCACGTAATCGGTTTTGGCTGATTATTTTCGCCGCCCCAGCCAGTcatta                                                                       >  1:813803‑M2/73‑141 (MQ=255)
                                                                      tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGa                                                                       >  2:451666‑M2/73‑143 (MQ=255)
                                                                      tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGa                                                                       >  1:385768‑M2/73‑143 (MQ=255)
                                                                          aaaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTAAggaaggtgcgaataggcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccga                                                                   <  2:468053‑M2/143‑72 (MQ=255)
                                                                           atgaaccttgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTggg                                                                  <  2:469391‑M2/76‑1 (MQ=255)
                                                                           atgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCCGTCAGTAATGACgggg                                                                  >  2:910219‑M2/68‑143 (MQ=255)
                                                                             gaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAGTGGGGc                                                                >  2:540827‑M2/66‑143 (MQ=255)
                                                                              aaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGGCg                                                               <  2:283634‑M2/79‑1 (MQ=255)
                                                                              aaccctgtgctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGGCg                                                               <  2:760713‑M2/79‑1 (MQ=255)
                                                                                   tgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTACTGGGGCGttttt                                                          >  1:631138‑M2/60‑143 (MQ=255)
                                                                                    gttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGAGtttttt                                                         >  1:464902‑M2/59‑143 (MQ=255)
                                                                                     actaaactcaacAGATAAGAGTAAAGTCAGACGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctccc                                                        >  2:860785‑M2/2‑61 (MQ=255)
                                                                                      tctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTAt                                                       <  1:265627‑M2/87‑1 (MQ=255)
                                                                                      ctgaactgaaCAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcgggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgt                                                       >  2:702182‑M2/1‑60 (MQ=255)
                                                                                       ctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTAtt                                                      >  1:717318‑M2/56‑143 (MQ=255)
                                                                                             gaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgc                                                >  1:27001‑M2/1‑53 (MQ=255)
                                                                                               gcaGAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgcct                                              >  1:413691‑M2/2‑51 (MQ=255)
                                                                                               acAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgcct                                              >  1:817247‑M2/1‑51 (MQ=255)
                                                                                                 aaaaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgagtttttctcccgtaaatgccttg                                            <  2:725500‑M2/143‑95 (MQ=255)
                                                                                                  gatgacaaacaggatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGTATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaagaa                                           <  1:368312‑M2/99‑1 (MQ=255)
                                                                                                   aaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaa                                          <  2:708557‑M2/143‑97 (MQ=255)
                                                                                                   aaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaa                                          <  2:615498‑M2/143‑97 (MQ=255)
                                                                                                    tgacaaacatgtgctcatacgagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                                         <  2:699172‑M2/101‑1 (MQ=255)
                                                                                                    aaaGAGTCACGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaat                                         >  1:259418‑M2/1‑46 (MQ=255)
                                                                                                      acaaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagacaaga                                       >  1:366157‑M2/41‑143 (MQ=255)
                                                                                                       caaacatgatctcatatcagggacttgttcgcaccttccCTAACATCCAGACCAAAAAAATAACGAGATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGAt                                      <  1:569429‑M2/104‑1 (MQ=255)
                                                                                                        agTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagc                                     >  1:128121‑M2/1‑42 (MQ=255)
                                                                                                        aaacatgatctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGCTTTGGCTGCATATTTTAGACGCCCAAGACAGTGATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATc                                     >  2:670818‑M2/39‑143 (MQ=255)
                                                                                                           aaaGTTAGTCGCGTAGGGTACAGCGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgcttttttctcccgtaaatgccttgaatcaccctc                                  <  2:18615‑M2/143‑105 (MQ=255)
                                                                                                            aaGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcgtgctat                                 <  1:784453‑M2/143‑106 (MQ=255)
                                                                                                             aGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctctcgtaaaggccttgaattagcctctt                                <  1:164039‑M2/143‑107 (MQ=255)
                                                                                                              gTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcattttagttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctattt                               <  1:664828‑M2/143‑108 (MQ=255)
                                                                                                                 ctcatatcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGcc                            <  2:385768‑M2/114‑1 (MQ=255)
                                                                                                                 aGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggagattcttctcggctgactcagtcatttcattgcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttaga                            >  2:617035‑M2/1‑33 (MQ=255)
                                                                                                                 aGTCGCGTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttaga                            >  1:360781‑M2/1‑33 (MQ=255)
                                                                                                                     gcgTAGGGTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcacactatttagaccgt                        <  2:618686‑M2/143‑115 (MQ=255)
                                                                                                                      atcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGCATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATg                       >  1:736057‑M2/25‑143 (MQ=255)
                                                                                                                      atcagggacttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATg                       <  1:539027‑M2/119‑1 (MQ=255)
                                                                                                                          gggTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttctt                   >  1:450762‑M2/1‑24 (MQ=255)
                                                                                                                           ggTACAGAGGTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttc                  >  1:576728‑M2/1‑23 (MQ=255)
                                                                                                                           ggAACAGAGGTAAGATGTTCTATggaaggtgcgaacgagcggggcaattctgctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttc                  >  1:124802‑M2/1‑23 (MQ=255)
                                                                                                                              cttgttcgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAGAGAATAGATGCGTAATGCCTGATGCGATTTGt               >  2:290611‑M2/17‑143 (MQ=255)
                                                                                                                                    gTAAGATGTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaa         <  1:489309‑M2/143‑130 (MQ=255)
                                                                                                                                    cgcaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGACTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATACCTGGGGCGTTTTTTATTGAGCGCAAGAAAAGATCCGTAATGCATCATGCGCTATGTTTATCa         >  2:600295‑M2/11‑143 (MQ=255)
                                                                                                                                     gaaccttccCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCTCTCTAGACAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGACTGATGCGCTATGTGTATCAg        >  1:35487‑M2/10‑143 (MQ=255)
                                                                                                                                           gTTCTATggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgtta  >  1:270957‑M2/1‑7 (MQ=255)
                                                                                                                                              |                                                                                                                                           
AGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAAC  >  NC_000913/4049781‑4050061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.