Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,065,571	T→A	17.1%	T66S (ACC→TCC)	yihO ←	putative sulphoquinovose importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,571	0	T	A	17.1%	211.8 / 5.8	95	T66S (ACC→TCC)	yihO	putative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base T (58/16);  new base A (4/14);  total (64/31)
Fisher's exact test for biased strand distribution p-value = 1.49e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTAT  >  NC_000913/4065348‑4065803                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            atcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTaaaaa                                                                                                                                                                                                                              >  1:13908/1‑238 (MQ=255)      aCCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCg                                                                                                                                                                                                                      <  1:864021/239‑1 (MQ=255)       ccAGACGAACAGTTCAACAGACTGTACGAAAGTCCAAACATCATAAACAGCCCGGTGGCGATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGc                                                                                                                                                                                                                     <  1:373574/239‑1 (MQ=255)        cATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGATGCATCTAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGCAAAAATCCGGTGAGCATATAGGTAAAC‑GCGGTAAAAAA‑CT‑TCGcc                                                                                                                                                                                                                    >  1:182762/1‑239 (MQ=255)          tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTCAAAAA‑AT‑TCGcga                                                                                                                                                                                                                   >  1:821104/1‑236 (MQ=255)          tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGccac                                                                                                                                                                                                                  <  1:959432/239‑1 (MQ=255)          tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGcca                                                                                                                                                                                                                   >  1:1080995/1‑238 (MQ=255)           aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAG‑GCGGTAAAAAA‑CT‑TCGccacc                                                                                                                                                                                                                 >  1:1235587/1‑239 (MQ=255)             gAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAg                                                                                                                                                                                                               <  1:1141864/239‑1 (MQ=255)             acgCAGTTCAACAGACTGTTTGAAAGACCAAACATCATAAACAGCGCGGTTGCAATCGTCGTTTTAACCGGCAACAAAAAGGT‑GGTGGCGATAAATTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTGTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCTCCAg                                                                                                                                                                                                               <  2:13908/237‑1 (MQ=255)                cAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGaaa                                                                                                                                                                                                            >  1:38619/1‑239 (MQ=255)                  gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑GGGCC‑CAATATTTTTCCG‑CGAGTAGGGTAAAAATCCGGGAAGCAAATCGGAAAAC‑GCGGTAAAAAA‑CT‑TCGCAACAAAAAAGa                                                                                                                                                                                                          >  2:651645/1‑239 (MQ=255)                  gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATCTCGGTAAAC‑GCGGGAAAAAA‑CT‑TCGCCACCACaaaaa                                                                                                                                                                                                          >  2:1141864/1‑237 (MQ=255)                  gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCGGAAAGa                                                                                                                                                                                                          >  1:697260/1‑239 (MQ=255)                  gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGA‑GGAGGCGATAAACTGGAGCG‑TTGCAATTAACTCCGC‑CGG‑AACTGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGTTAAAAAA‑CT‑TCGCCACCATAAAGa                                                                                                                                                                                                          >  1:752005/1‑239 (MQ=255)                     catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGTGTAAAAATCCGGTGAGCATATCGGAAAAC‑GCGGTAAAAAA‑AT‑TCGCCACCAAAAAGaaaa                                                                                                                                                                                                       >  2:959432/1‑239 (MQ=255)                             tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAACATAAA‑G‑GGACAAAATTTGCCTTT‑AAGCC‑CAATATTTTTCCG‑CGAGTCGAGAAAAAAACCGGGAAGCGTAACGGGAAAC‑GCCGGAAAAAA‑AT‑TCCCCAACAAAAAGaaaacacccaca                                                                                                                                                                                               >  2:1304304/1‑231 (MQ=255)                             tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCa                                                                                                                                                                                               >  1:935501/1‑239 (MQ=255)                             tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCAGGTAAAAATCCGGTGAGCATATCGGAAAAG‑CCGGTAAAAAA‑CT‑TCGCAACAAGAAAGATAATCCCGCCa                                                                                                                                                                                               >  1:587945/1‑239 (MQ=255)                               tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGATCG‑TTGCAATTAACGCCGC‑CGG‑AACCGATGCGTATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCT‑CGAGTCGAGTAAAAAACCGGGTAGCATATCGGCAAAC‑ATGGTAAAAAA‑CT‑TGCCCAACAAAAAGACaaacaccccaca                                                                                                                                                                                             >  2:718950/1‑229 (MQ=255)                               tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTGAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATa                                                                                                                                                                                             >  1:330125/1‑239 (MQ=255)                               tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATa                                                                                                                                                                                             >  1:293988/1‑239 (MQ=255)                               tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑AAATATTTTTCCG‑CGAATAGAGTAAAAATCCGGTGATCATACCGGAAAAC‑CCGGACAAAAA‑CT‑TCGCCACCAAAAAGAACAACCCGCCaaa                                                                                                                                                                                             >  2:338216/1‑237 (MQ=255)                                       tCCAATCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGccac                                                                                                                                                                                                                  <  1:793493/210‑1 (MQ=255)                                       tCCAATCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGccac                                                                                                                                                                                                                  >  2:793493/1‑210 (MQ=255)                                         cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAg                                                                                                                                                                                                           >  1:265810/1‑215 (MQ=255)                                         cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                   >  1:716765/1‑239 (MQ=255)                                         cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                   >  1:271177/1‑239 (MQ=255)                                         cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGGGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                   >  1:1177027/1‑239 (MQ=255)                                           aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGCGCATATCGGTAAAC‑GCGGGAAAAAA‑CT‑TCGCCACCGGAAAGATAATCCAGCCATAGTAAGCAGGCa                                                                                                                                                                                  >  1:907809/1‑238 (MQ=255)                                                catAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCACCCCTa                                                                                                                                                                            >  1:564390/1‑239 (MQ=255)                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCCGAAAGATAATCCCGCCATAGTAACCAGGCACCCCTAATTCATCg                                                                                                                                                                    >  1:34859/1‑239 (MQ=255)                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAAtt                                                                                                                                                                         >  1:861180/1‑234 (MQ=255)                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                    >  1:458218/1‑239 (MQ=255)                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGAATATCGGTAACC‑GCGGTACAAAA‑AT‑TTCCACCCAGAAAGAAAATCCCCCCAAAATAAGCAGCAAACCCTAAATcccag                                                                                                                                                                    >  2:49615/1‑235 (MQ=255)                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAAGATTTTTCCG‑CGAGTCGATTAAAAAACCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCACAACGAAAATCCCGCCAAAGAAAGAAAGCACACCTaaacaacag                                                                                                                                                                    >  2:413082/1‑232 (MQ=255)                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGGTAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                   >  1:881549/1‑239 (MQ=255)                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                   >  1:746514/1‑239 (MQ=255)                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                   >  1:595067/1‑239 (MQ=255)                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGACAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAc‑g                                                                                                                                                                                                                                       >  1:549957/1‑173 (MQ=255)                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGACAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAc‑g                                                                                                                                                                                                                                       <  2:549957/173‑1 (MQ=255)                                                          gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGt                                                                                                                                                                  >  1:1300143/1‑239 (MQ=255)                                                           cgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCGCCCTAAGTAAGCAGGCACCCCTAATTCATCGGta                                                                                                                                                                 >  1:501482/1‑239 (MQ=255)                                                             ggtccactcgttgTTTTACCCGGAAAACAAAGTGT‑GGTTGAGAAAAACTGGACCG‑TTGAAATTAACCCCGC‑CGG‑ACCCGTAGCATAAAAAGTAAA‑G‑GAAAAAATTTTGCCTTT‑GGCCC‑AAATATTTGTCCG‑CGAGTCGAAAAAAACTCCGGTGATCATATCGGTAAAC‑GAGATAAAAAC‑CT‑TCTCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtat                                                                                                                                                                <  2:1300143/231‑1 (MQ=255)                                                                     tgttcttttAACCCGAAAAACAAAGGTGGGTGCCAAAAAAGTGAAGCG‑TGTAAATCAGCG‑CGC‑CGGCACCCGTTGTATATAAAAAAAG‑G‑GGACGAAATGTCTTTTT‑AGGCC‑AAATATTTTTCAC‑AGAGTCGAGAAAAATTCCGGTGAGCATTCCGGAAAAC‑‑CGGTAAAAAATCT‑GCACCACGAGAAAGAAAACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                        <  2:752005/234‑1 (MQ=255)                                                                      tgttttttAACCGCCAAAAAAAGGGG‑GGGGGCAATAAAAGGGAGCG‑TTACAATTACCCGCGC‑AGG‑ACCCGCTTAAAAAAAAAAAAA‑G‑GGAAGAAATTTGGCTTT‑AGGCC‑CAATATTTTTCCG‑CGATACGAATAAAAAGCCGGTGAAAATATCGTAAAAC‑GTGGTAAAAAT‑CT‑TCGCCACCAGAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                      <  2:716765/235‑1 (MQ=255)                                                                      gtttttttAACCGCAAAAGAAAATGG‑GGGGGCAAAAAAATGGAGTG‑GATCAATTACGCCCGC‑CGG‑AACCG‑TGTAAAAAAAAAAAAGG‑GGAAGAATTTGTCGTTT‑AGGCC‑CAATATTTGCCCG‑GGAGTCGAATAAAAACCCGGTGATAATATCGGAAACC‑GCGGAAAAAAT‑CT‑CCGCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                      <  2:427868/235‑1 (MQ=255)                                                                       ttgtTTTTACCCGGAAAAAAAAGGG‑GGTGGGGAAAAAAGGGGGCG‑TTGCAAATTACGCCGCGCGA‑AACGGTTGATTAAAAAAAAAA‑G‑GGA‑AAAATTTGGCTTTGAGGCC‑AAATATTTTTGCG‑CGAGTGGAGTAAAAACCCGGTGAGCTTTTCGGAAAAC‑GCGGAAAAAAC‑CT‑TCGCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                      <  2:1257455/237‑1 (MQ=255)                                                                        cgtTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTTTAAAATTTgagga                                                                                                                                                    >  1:361330/1‑239 (MQ=255)                                                                                ccGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgaggagcaaaaag                                                                                                                                            >  1:763914/1‑232 (MQ=255)                                                                                 cGGAAAACAAAAGGT‑GGT‑GCCAAAAACTGGGGGGTTTGCAATAAACGCCGC‑CGA‑AACCGCTGAAAATAAAATAAA‑G‑GGACGAATTTTGCCTTT‑AGGCC‑AAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGAAAAAAA‑CT‑TCGCCACAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACagag                                                                                                                                           <  1:206443/239‑1 (MQ=255)                                                                                    caaacaaaAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCACAGTAAGCAGGCAACCCTACTTCATCGGAGTAAAATTTGAGGAAATaaaaagaa                                                                                                                                         >  2:1179303/1‑231 (MQ=255)                                                                                                  gtggCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATCGTAAGAAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCAt                                                                                                                           >  1:796883/1‑239 (MQ=255)                                                                                                  gtggCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATACAATTTGAGGAGATACAGGGTACCGCAGCAGAGCAt                                                                                                                           >  1:463469/1‑239 (MQ=255)                                                                                                    ggCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGa                                                                                                                                                                                                              >  2:54502/1‑154 (MQ=255)                                                                                                    ggCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGa                                                                                                                                                                                                              <  1:54502/154‑1 (MQ=255)                                                                                                        aTAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGGATAAAATTTCAGGAGATCCACAGTACCCGTGCCGAGCATTAAAt                                                                                                                      >  1:1285478/1‑238 (MQ=255)                                                                                                         tAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTc                                                                                                                    >  1:543928/1‑239 (MQ=255)                                                                                                          aaaCTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCg                                                                                                                   >  1:901282/1‑239 (MQ=255)                                                                                                                gAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGGATAAAATTTGAGGAGATACAAAGAACCGATGCAGACAATTAAATTGAAAcca                                                                                                              >  2:268499/1‑237 (MQ=255)                                                                                                                      ttGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGGAATAAAAAGTACCGATGCAGAGAATTAAATTCGAAacgacgac                                                                                                         >  2:526885/1‑236 (MQ=255)                                                                                                                       tGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCg                                                                                                        >  1:447570/1‑238 (MQ=255)                                                                                                                                cgccgc‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTAATCGGTATACAATTTGAGGAGATACAGAGTACCg                                                                                                                                      >  1:891897/1‑199 (MQ=255)                                                                                                                                 gccgc‑cGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAACGATGAAGAGAATTAAATTCGAACCGAAGTCCCccaaacaaaa                                                                                             >  2:299098/1‑235 (MQ=255)                                                                                                                                     c‑cgG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCTAACCGAAGGCCCccaaacaataaggg                                                                                         >  2:723233/1‑235 (MQ=255)                                                                                                                                                   taaataaaaaaaa‑GAGGAGAAAATTTTCCTTT‑GAGCC‑CAAATTTTTTCCGTGGAGTCGAGTAAAAATAGTGTGAGCAT‑GCAGAAAAA‑GCGGAAAAAAA‑TTCCCGCACCCAAAAAGAAACGCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTccc                                                                               <  2:15198/236‑1 (MQ=255)                                                                                                                                                    atatAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCa                                                                            >  1:15198/1‑239 (MQ=255)                                                                                                                                                        aaaaaaag‑G‑GGAAAAATTTGGCCTTT‑AGGCC‑AAATTTTTTCCGG‑GGAGTCGAGAAAAAACCCGGGAAGAATAGCGGTAACC‑GGGGAAAAAAA‑CT‑CCGCCACCAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                        <  1:268499/239‑1 (MQ=255)                                                                                                                                                        aaaaaaaa‑G‑GGAAAAATTTTGCTTTT‑AAGCA‑CATTTTTTTCCCG‑CAATCCAAGAAAAAACCCGAGGAACTACTCGGAAAAC‑GAGGAAAAAAT‑CC‑TCCCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGGCGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                        <  2:458218/239‑1 (MQ=255)                                                                                                                                                        aaaaaaaa‑G‑GAAAGAAATTTGCCTTT‑AGCCC‑AAATATTTTCCCG‑GGAGTCGAGAAAAAATCCGGGGACGAATTGGGTAAAC‑GGGGTAAAAAA‑CT‑CCCCCCCAAGAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                        <  1:428683/239‑1 (MQ=255)                                                                                                                                                          aataaa‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGAAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGTCGATTTTTTCCCGCAGATTCa                                                                      >  1:229054/1‑239 (MQ=255)                                                                                                                                                               a‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                 >  1:624389/1‑239 (MQ=255)                                                                                                                                                                    gACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATCCCATAGGCGATTTTTTCCCCCACATTCAGTTTTAAt                                                              >  1:1086987/1‑239 (MQ=255)                                                                                                                                                                      cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATTCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTACCGCCAATTCAGTTTTCATGt                                                            >  2:156052/1‑239 (MQ=255)                                                                                                                                                                      cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGt                                                            >  2:135602/1‑239 (MQ=255)                                                                                                                                                                      cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCACGCCGATTCAGTTTTAATg                                                             >  1:1083506/1‑238 (MQ=255)                                                                                                                                                                         attttGCCTTT‑ATGCC‑CAATTTTTTCCCG‑CGTGCGGAGAAAAAATCCGGGAATCATATCGGAAACC‑GCGGTAAAAAC‑CT‑TCGCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAg                                                         <  1:413082/237‑1 (MQ=255)                                                                                                                                                                              tgtttt‑GGGCC‑AAATTTTTTCCGG‑GGAATCGAAAAAAAACCGGGGGAGAATTGGGGAAAAC‑GCGGTAAAAAC‑CT‑CCGCAACAAGAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGAt                                                    <  1:526885/236‑1 (MQ=255)                                                                                                                                                                               ccTTG‑AGCCA‑CAATTTTTTCCCG‑GGAGTCAAAAAAAAATCCGGTGAGCATATGGAAAAAC‑GCGAAAAAAAC‑CT‑CCCCCACCAAAAAGATATCCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGAtt                                                   <  1:21196/239‑1 (MQ=255)                                                                                                                                                                                 ttt‑AGGCCACAATTTTTTCCCG‑GAAGTGGAAAAAAATCCCGGTGACAATACCGGAAAAC‑GCGGTAAAAAC‑CT‑TCCCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTa                                                  <  1:1304304/239‑1 (MQ=255)                                                                                                                                                                                     agccc‑cATTTTTTTCCCG‑GGAGTCAATAAAAAATCCGGGAAGATTATCGGTAAAC‑GGGAAAAAAAA‑CT‑TCCCCCCAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                              <  1:723233/236‑1 (MQ=255)                                                                                                                                                                                     aGGCC‑TAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGCAGATATACAGAGTAACGATGCAGGGCATTAAATTCTAACCGCCGTGGCCCATACCATGGGGGCTTTTTTCCCCGAAATTCCGTTTTAACGTCAAGTGATTATgtg                                              >  2:750390/1‑237 (MQ=255)                                                                                                                                                                                     aGGCC‑AAATTTTTTTCCG‑CGAGTCGAGTAAAAATCCGGGAAGAATTCCGGTAAAC‑GCGATAAAAAC‑CT‑TCCCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                              <  1:651645/239‑1 (MQ=255)                                                                                                                                                                                        aacaaaTTTTTTCCCG‑GGAGTGGAGAAAAAGTCGGGGGAGAATATCGGAAAAC‑GGGGTAAAAAC‑CT‑CCACCACAAGAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCa                                            <  1:1286880/235‑1 (MQ=255)                                                                                                                                                                                                   ttCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TAGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATc                                   >  1:145123/1‑237 (MQ=255)                                                                                                                                                                                                     cccgagagcggagtAAAAGTCCGGTGAGCATACCGGTAAAC‑GGGGTAAAAAC‑CT‑TCACCACCAAAAAAATAACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                <  1:1179303/229‑1 (MQ=255)                                                                                                                                                                                                         cgagtcgagtAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATATTCACTCCtt                            >  1:820610/1‑239 (MQ=255)                                                                                                                                                                                                         cgagtcgagtAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGCCGATTTTTTCCCGCAGATTACGTTTTAATGTAAGTGGATTATGGTCAGACATAATCAcctt                              >  2:1286880/1‑234 (MQ=255)                                                                                                                                                                                                                      actCCGGTGAGCATATCGGAAACC‑GGGGAAAAAAC‑CT‑TCGCCACCAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCa               <  1:299098/237‑1 (MQ=255)                                                                                                                                                                                                                           ggTGAGTATATCGTAAAAC‑AGGTTAAAAAC‑CG‑TCGCCCCCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg          <  1:349800/239‑1 (MQ=255)                                                                                                                                                                                                                            gTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCACCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCATTTTTAATGTCAGTGGATTATGGTCAGACATAATAACTCCTTATAACGCACCGCAGGGcgt         >  1:416397/1‑239 (MQ=255)                                                                                                                                                                                                                             tGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCCCCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAtt                                                                                                                                                          <  1:806756/93‑1 (MQ=255)                                                                                                                                                                                                                             tGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAtt                                                                                                                                                          >  2:806756/1‑93 (MQ=255)                                                                                                                                                                                                                                   tgccGGAAAAC‑GCGAAAAAAAC‑CT‑CCCCAAGAAGAAAAAGAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat  <  2:1235587/236‑1 (MQ=255)                                                                                                                                                                                                                                    atCGGGAAACGGAGGAAAAAAC‑CT‑TCGCCACAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat  <  1:535661/239‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            ATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCGATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAC‑GCGGTAAAAAA‑CT‑TCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTAT  >  NC_000913/4065348‑4065803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑