BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,072,425	T→C	8.8%	E76G (GAG→GGG)	yihT ←	6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,072,425	0	T	C	8.8%	393.9 / 2.8	163	E76G (GAG→GGG)	yihT	6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand): ref base T (70/71); new base C (3/15); total (74/89)
Fisher's exact test for biased strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

ACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTA  >  NC_000913/4072188‑4072658

  cAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTACTCCCTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑a‑a                                                                                                                                                                                                                                            <  1:706269/239‑1 (MQ=255)

  cAGACCGTGTGAGGTGCACAGTTCGTTGAACTCTTTCGCCATATCCAGACGTTGCAGCGCAGCTTAATCACTACGCCACAGCCCCAGCAGTTTTAAGGCTTTGCCACCGTCGTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑a‑a                                                                                                                                                                                                                                            <  1:751941/239‑1 (MQ=255)

     aCCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTTCTC‑A‑acc                                                                                                                                                                                                                                          >  1:1101389/1‑238 (MQ=255)

     aCCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CCCGTTTTGCTT‑A‑acc                                                                                                                                                                                                                                          >  1:1306972/1‑238 (MQ=255)

         tgtgAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCCTGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTg                                                                                                                                                                                                                                     >  1:827015/1‑239 (MQ=255)

         tgtgAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGGTC‑A‑ACCACCTg                                                                                                                                                                                                                                     >  1:524182/1‑239 (MQ=255)

         tgtgAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTAAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGC‑A‑CACAACATTGC‑GCA‑AATTTTTGCAAT‑CGTCTTTTGCTC‑A‑ACCACCTg                                                                                                                                                                                                                                     >  1:1210776/1‑239 (MQ=255)

            gAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGc                                                                                                                                                                                                                                   <  1:777046/238‑1 (MQ=255)

               tGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGTCCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCCTGGC‑GCA‑ACTTTTGGCAAA‑CGCGTTTTCGCC‑A‑ACCCCCTGGCggga                                                                                                                                                                                                                               >  1:138936/1‑237 (MQ=255)

               tGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACCCATCGGCGGCGACCA‑ACAATGCC‑GAA‑ACTTTT‑GAAAA‑CAGGTTTTGCTCAA‑ACCACCTGggggg                                                                                                                                                                                                                                >  2:1151891/1‑238 (MQ=255)

                ggCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGCATACCGTTGCCAGGAATGACCTCATCGGCGGCGC‑CC‑ACAATGGC‑CCC‑ACTCTTGGCAAT‑CGCAGTTTGCTC‑C‑ACACCCTGGCggacg                                                                                                                                                                                                                              >  1:611399/1‑236 (MQ=255)

                 gagcaGTTCGGTGAACTCTTTCCCCATAACCAGACGTTGCTGCCCATCTTCATCCCTTCGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAg                                                                                                                                                                                                                              <  1:1031194/235‑1 (MQ=255)

                    atgttttttGAACTCTTTACCCATATCCATTCGTTGGTTCGCATCGTCATCCCGTCGGCACAGCACCTGCAGTTTTAAGGCTTTGCCACCGTCTTGTTCGATCTGTAGCGGATTGATTTTGCGGTCAATAACCGCGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑AGCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCCC‑A‑ACCACCTGGCGGTAGCAg                                                                                                                                                                                                                           <  2:109622/231‑1 (MQ=255)

                        tCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCCCTACGCCAAAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AAtt                                                                                                                                                                                                                      <  1:650838/238‑1 (MQ=255)

                            tGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGa                                                                                                                                                                                                                 >  1:305022/1‑239 (MQ=255)

                                   tttCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTTGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTCGCAG‑AATTTTTGATCTACC‑a                                                                                                                                                                                                         >  1:1161918/1‑239 (MQ=255)

                                    ttCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CC‑ATCATGGC‑GCC‑ACTTTTTGCAAT‑CCCGGTTTTGTC‑A‑ACCACCCGGCGCGAGCCA‑AATTTTTGATcatccaa                                                                                                                                                                                                         >  2:20107/1‑233 (MQ=255)

                                       aCCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCa                                                                                                                                                                                                                            <  1:67967/218‑1 (MQ=255)

                                           tatCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CC‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGCTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATctc                                                                                                                                                                                                  >  1:137839/1‑236 (MQ=255)

                                           tatCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGc‑c                                                                                                                                                                                                >  1:251917/1‑239 (MQ=255)

                                           tatCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGc‑c                                                                                                                                                                                                <  1:20107/239‑1 (MQ=255)

                                           tatCCAGACGTTGCCGCGCATCTTCATCCCTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGc‑c                                                                                                                                                                                                <  1:1089288/239‑1 (MQ=255)

                                             tCCAGTCGTTGCTGCGCATTGTCATCCCTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTTGTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCgg                                                                                                                                                                                                                                 <  2:740196/207‑1 (MQ=255)

                                             tCCAGGCGTGGTTGCGCTTCTTTATCCCTTTGCCACAGCACCAGTCGTTTTAAGGCTTTGCCACCGGTTTGTTTGATCTGTAGGGGATTGATTTTGGGGTTAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGa                                                                                                                                                                                              <  2:724205/239‑1 (MQ=255)

                                             tCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCAC‑CA‑ACCATGGG‑CCACATTTTTGAAAAC‑CGGGTTTTGC‑C‑A‑ACCCCCCTCCGGGAGCAG‑CATTTTGGATTTACC‑AGAATTGc‑cca                                                                                                                                                                                              >  2:908069/1‑237 (MQ=255)

                                                 gACGGTGCTGCGCATCGTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCa                                                                                                                                                                                          <  1:1011002/239‑1 (MQ=255)

                                                      tgctccGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTAATTTTGCGGTCAATAACCACGCTATCACCCGGAAAACCGTTGCCAGGAATGAACTCATCGCCGGCGC‑CC‑ACCATGGCTACA‑CCTTTTTG‑AAT‑CAGGGTTTTCTC‑C‑ACCCACCCGCCGTTGCAC‑AATTGTTGATCGACA‑AGAATCAc‑caaacaacaaag                                                                                                                                                                                     >  2:1292895/1‑228 (MQ=255)

                                                       gctgcGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGGATGACATCATCGGCGGCGA‑CA‑ATCATAGC‑GCC‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCCGGCGGTGAGGC‑AATTTTTTTTCTACC‑ATAAACGC‑CGAGGCaaaaagg                                                                                                                                                                                    >  2:920416/1‑236 (MQ=255)

                                                       gctgcGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTGGCAG‑AATTTTTGGTCTCCC‑AGAACTCC‑CGAGGCCTAAGGc                                                                                                                                                                                    >  1:5438/1‑239 (MQ=255)

                                                       gctgcGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCCTAAGGc                                                                                                                                                                                    >  1:285095/1‑239 (MQ=255)

                                                           cgcATCTTCATCCCTACGCCACATCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTTATCTGTAGCGGATTGATTTTGCGGTTAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCgaga                                                                                                                                                                                <  1:1311588/239‑1 (MQ=255)

                                                           cgcATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGC‑CA‑ATCATGGC‑GCA‑ACCTTTGGCAAT‑AGCGTTTTTCTT‑C‑ACCACCTGGCGGCAGCAGAAATTTTTGTTCTA‑C‑AGAATCCC‑CGAGGCaaacaggggga                                                                                                                                                                                >  2:418236/1‑229 (MQ=255)

                                                           cgcATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑AATTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAgggcaga                                                                                                                                                                                >  1:907032/1‑234 (MQ=255)

                                                            ccATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGagag                                                                                                                                                                               <  1:801328/238‑1 (MQ=255)

                                                                   catcaCTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCttt                                                                                                                                                                        <  1:1265973/239‑1 (MQ=255)

                                                                   catcaCTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCttt                                                                                                                                                                        <  1:418236/239‑1 (MQ=255)

                                                                       aCTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTGGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑a                                                                                                                                                                                                                                              <  1:1045296/169‑1 (MQ=255)

                                                                          aCGCCACAGCACCAGCGGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGGTTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑CCA‑ACTTTTGGCAAA‑CGCGTTTTTCTT‑A‑AACACCCGGCGGGGACCG‑AATTGTTTATTTACC‑CAAAACCC‑ACAAGCCAAAGGCGATAGGGCATTTGCAgggg                                                                                                                                                                 >  2:217344/1‑236 (MQ=255)

                                                                          aCGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CACGTTTTGCTC‑A‑ACACCCTGGCGGAAGCCG‑AATTGTTGATCTACC‑AGAATCGC‑CACGGCCTAAGGCGACGGGGCCTTTCCAACgg                                                                                                                                                                 >  1:1084240/1‑238 (MQ=255)

                                                                           cGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CC‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑C‑ACCACCCTGCGGGAGCGA‑AATTTTTTATTTACC‑CAAATCCC‑CCAAGCAAAAAGCGGGAGGGGCTTTTCAGCGtt                                                                                                                                                                >  2:1269080/1‑239 (MQ=255)

                                                                           cGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGTCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAAAGGGCCTTTGCACCGtt                                                                                                                                                                >  1:582324/1‑239 (MQ=255)

                                                                           cGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATTTACC‑AGAATCGC‑CGAGGCCTAAGGCGAGAGGGCCTTTGCAGCGtt                                                                                                                                                                >  1:712678/1‑239 (MQ=255)

                                                                              cacaGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑CCTTTTGGCAAT‑CGCGCTTTGGTC‑A‑ACCACCTGGCGGGCGCCG‑AATTTTTGATCTCAC‑AAAAACGG‑CCAGGCCAAAGGGGAGGGGGACGTTTCAGAGTTa                                                                                                                                                               >  2:684283/1‑237 (MQ=255)

                                                                                 aGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAATGCGAGAGGGCCTTTGCAGCGTTAACttt                                                                                                                                                          >  1:801915/1‑239 (MQ=255)

                                                                                     ccatcttttttAAGGTTTTGTCATCCTTTTGTTTAGTCTGCAGCGTGTTGATTTTCAGGCCACTACCCCCTCTATCGACCGTAACACCGGTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGTAAT‑CGCGTTTTGCTC‑A‑ACCACTTGTCGGTAGCTG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAAGGCGATTGGATCGTAAACGTTGaaa                                                                                                                                                      <  2:595094/232‑1 (MQ=255)

                                                                                     ccAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AAAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTTaat                                                                                                                                                      >  1:702009/1‑238 (MQ=255)

                                                                                        gcagTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCC‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTTGCGGTAGCAA‑GATTGTTGATCTACC‑CGAATCGC‑CCAAGCACAAGGCGAGAAGGCCTTTTGAGCGTTAAAATTGAAAACa                                                                                                                                                   >  2:258959/1‑239 (MQ=255)

                                                                                        gcagTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGGAGCAG‑AATTGTTGATCTAAC‑AGAATCGC‑CGGGGCATAAGGCGAGAGGGCCTTTGGAGCGATCACTTTGGAATCa                                                                                                                                                   >  1:1023233/1‑239 (MQ=255)

                                                                                               tAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCAGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTaaaa                                                                                                                                            <  1:897269/239‑1 (MQ=255)

                                                                                                 aGGCTTTGCCACCGGCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACg                                                                                                                                          <  1:844394/239‑1 (MQ=255)

                                                                                                     tttCCCCCCGTCTTGTTTGTCCGGTTGCGGTTTGTTTTTGGGGTCAATAACCCCGCTACCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTa                                                                                                                                       <  2:110237/238‑1 (MQ=255)

                                                                                                      ttGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGG‑CA‑AACCAGGT‑GCA‑ACTTTTGGCAAG‑CGCGGTTTGCTC‑ACACCCCCT‑CCGGGTGCAG‑AATGTTTGTCCTACC‑AGCTTCTC‑CGAGGCCACAGGCGCGGAGGCCCTTGCCGCGTTAACGTTTAAAACAGTTAAAAcacaacc                                                                                                                                     >  2:297109/1‑233 (MQ=255)

                                                                                                      ttGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTTTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATc                                                                                                                                     >  1:965016/1‑239 (MQ=255)

                                                                                                      ttGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCCAAAGGCGAGAGGGCCTTTCCAGCGTTCACTTTGAAATCCGTTAAAAAGCCATc                                                                                                                                     >  1:744018/1‑239 (MQ=255)

                                                                                                        gCCACCTTCTTGTTTGATCTGTAGCGGATGGATTTTGCGGGCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑AA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCgg                                                                                                                                   <  2:285095/239‑1 (MQ=255)

                                                                                                           aCCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCAAGCTATCGACCGGAATCCCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTa                                                                                                                                <  1:297109/239‑1 (MQ=255)

                                                                                                                     ttgctttGTAGCGGATTGTTTTTGCGGTCACTAACCACGCTAGCGGCCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCggg                                                                                                                      <  2:830622/233‑1 (MQ=255)

                                                                                                                         tCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCgg                                                                                                                                                                                                                                 <  1:1219116/131‑1 (MQ=255)

                                                                                                                         tCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCgg                                                                                                                                                                                                                                 >  2:1219116/1‑131 (MQ=255)

                                                                                                                         tCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTTATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTTCAGCGTTAACTTTGAAATCAGTTAAAAAGCAATCGGCTAACGGAGCTGGGGc                                                                                                                   >  1:1144918/1‑238 (MQ=255)

                                                                                                                             tAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGc                                                                                                                                         <  1:832988/212‑1 (MQ=255)

                                                                                                                              aGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATTCCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGc                                                                                                             <  1:1157444/239‑1 (MQ=255)

                                                                                                                               ggggtttgtttgtGCGGTCAATAACCGCCAGAACGACCGGAATACCGGTCGCAGGACTGGACTCAGCGGGGGCGC‑AA‑ATCAGTGA‑GCA‑ACTTTTGACAAT‑CCCTGTTTCGTC‑A‑ACCACCTGGGGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCGTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTGCCGGAGCGGGTGCCCCAGcc                                                                                                            <  2:886254/234‑1 (MQ=255)

                                                                                                                               gCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGcc                                                                                                            <  1:1151891/239‑1 (MQ=255)

                                                                                                                                    ttgattTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTTGCCG‑AATTGTTGATCTACC‑CGAATCGC‑CCGGGCATAAGGCGAGAGGGGCTTTGCAGCGTTAACTTTGACATCAGCTAAAACGATATCGGGCACCGGGCCGGGTGcccaccccccggc                                                                                                       >  2:943122/1‑229 (MQ=255)

                                                                                                                                       attTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTTCAGCGTTAACTTTGAAATCAGGTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑aa                                                                                                    >  1:494589/1‑238 (MQ=255)

                                                                                                                                       attTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTAAC‑AGAATCGC‑CCGAGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTTAAATCAGTTCAAACGCTATCGGCTACCGGAACGGGGGCCCCAGCCCCGGC‑aa                                                                                                    >  1:586675/1‑238 (MQ=255)

                                                                                                                                           tGCGGTAAATTACCCAGCTTGCGGCCGGAATACCGTTGCCAGGAATGAATCCACCGGCGGCGA‑CA‑ATCATGGC‑CGA‑ACTTTTGGGAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAAcatc                                                                                               <  2:419192/239‑1 (MQ=255)

                                                                                                                                           tGCGGGCAAAATCCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGTAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAAcatc                                                                                               <  1:376645/239‑1 (MQ=255)

                                                                                                                                            gCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGagag                                                                                                                                                                               <  2:1029696/159‑1 (MQ=255)

                                                                                                                                                  caTAACCGCGCTTTGGCCCGGAATACCTTTTCCAGGAATGAACTTATCGGGGGCCA‑CA‑ATCAGGGC‑GAA‑CCTTGTGGCAAT‑CGCGTTTTGCTC‑A‑ACCTCCTGGGCGTAGCAG‑AATTGGTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCa                                                                                        <  2:625414/238‑1 (MQ=255)

                                                                                                                                                        cACGCTATCGACCGGAATTCCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCtt                                                                                  <  1:1243130/239‑1 (MQ=255)

                                                                                                                                                            cTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGACCGGGGGCCCCAGCCCCGGC‑AAACACCATTCGCATGGCtttcg                                                                               >  1:832248/1‑235 (MQ=255)

                                                                                                                                                                  aCCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGGAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CCAGGCATAAAGCGAGAGGGCCTTTGCAGCGGTAACTTTGTAATCAGTTAAAAAGCTATCGGCTACCGGAGCGGGTGCCCCAGCCCCGGCAAAACACCAT‑CGCATGGCCTTGCGCTGATc                                                                        >  2:1045244/1‑239 (MQ=255)

                                                                                                                                                                       aaTTCCGGTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGAcc                                                                    <  1:1028070/238‑1 (MQ=255)

                                                                                                                                                                             gTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAACGGGGGCCCCAGCCCCGGG‑AAAAATCAAGCGCATTGCTTCGCGCCGATCGACCGCCAGCa                                                             >  1:38770/1‑239 (MQ=255)

                                                                                                                                                                                           aCTCATCGGCGGCGA‑AA‑ATCATGGC‑CCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCCCCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGcc                                                <  1:870709/238‑1 (MQ=255)

                                                                                                                                                                                               aTCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCCACCGGGGCGGGTACCCCAGCCGCGGC‑AAAACACCTGCGCATGGCTCTGCGCGTCTCGACGCCCACCATGGCAAAAAAGccccacg                                           >  2:369909/1‑235 (MQ=255)

                                                                                                                                                                                                gcggcggCGA‑CA‑ATCAGGGC‑GCA‑ACTTTGGGCAAT‑GGCGTTTTGCTC‑A‑ACCTCCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATgc                                          <  1:375848/238‑1 (MQ=255)

                                                                                                                                                                                                      gcgA‑CA‑ATCATGGC‑GCA‑ACTTTGGGCAAT‑CGCGTTTTGCTC‑A‑ACCCCCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGcgcgcg                                     <  1:908069/238‑1 (MQ=255)

                                                                                                                                                                                                       gggcaa‑aTCATGGC‑CCA‑ACTTTTGGCAAT‑CGCTTTTTGCCC‑A‑ACCCCCGGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGcgcgcg                                     <  1:44508/233‑1 (MQ=255)

                                                                                                                                                                                                        gA‑CA‑ATCAGGGC‑CAA‑ACTTTTGGCAAT‑CGCGTTTTGCCC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTa                                   <  1:369909/238‑1 (MQ=255)

                                                                                                                                                                                                           cA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGGTTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAAt                                 <  1:470915/238‑1 (MQ=255)

                                                                                                                                                                                                                taTGGC‑CCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGt                            <  1:97447/238‑1 (MQ=255)

                                                                                                                                                                                                                 aTGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGGCCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGtt                           <  1:1045244/239‑1 (MQ=255)

                                                                                                                                                                                                                  cttggccA‑ACTTTTTGCAATCCGCTTTTTGCCC‑A‑CCCACGGGGGGGGAGCAG‑AATTTTTAATCTACA‑AAAATGCC‑CGGGGAAAAGGGGGGGGGGGCCGTTGCAGCGTTAACTTTGAAATCAGTAAAAACGCTATCGGCTCCCGGGGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGt                            <  2:1166638/233‑1 (MQ=255)

                                                                                                                                                                                                                   ggC‑GCA‑ATTTTTGGCAAT‑CGCTTTTTGCCA‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTCCCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGa                         <  1:684283/239‑1 (MQ=255)

                                                                                                                                                                                                                   ggC‑ACA‑ACTTTTGGCAAT‑CGCGTTTTGCCC‑A‑ACCCCCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGa                         <  1:554309/239‑1 (MQ=255)

                                                                                                                                                                                                                    gc‑gcA‑ACTTTTGGCAAT‑CGCGTTTGGCAC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGa                         <  1:1203198/238‑1 (MQ=255)

                                                                                                                                                                                                                     gcccA‑ACTTTTGGCAAT‑CGCGTTTTGCCC‑A‑ACCCCCTGGCGGTAGCAA‑AATTGTTGATCTACC‑AGAACCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGa                         <  1:1237114/235‑1 (MQ=255)

                                                                                                                                                                                                                     c‑gcA‑AGTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGAt                        <  1:943122/238‑1 (MQ=255)

                                                                                                                                                                                                                       gcA‑ACTTTTGGCAAT‑CGCGGTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATg                       <  1:454109/238‑1 (MQ=255)

                                                                                                                                                                                                                       gcA‑ACTTTGGGCAAG‑CGCGTTTTGGCA‑A‑ACCACCGGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATg                       <  1:172378/238‑1 (MQ=255)

                                                                                                                                                                                                                        cA‑ACTTGTGGAAAT‑CGCGTTTGGCTC‑A‑ACCACCGGGCGGTAGCAA‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATgg                      <  1:2049/238‑1 (MQ=255)

                                                                                                                                                                                                                        aa‑aCTTTTGGCATT‑GCCGTGTTGCCC‑A‑ACCACCTGGAGGTAGCAG‑AATTGTTGATACACC‑AGAATCGC‑CGAGGCATAAGGCGTGGGGGCCTGTGCAGCGTTAACTTTGAACGCAGTTAAAACGCTATCGGCTACGGGGGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGgt                     <  2:832248/238‑1 (MQ=255)

                                                                                                                                                                                                                                tgccAAT‑CTCTTTTTGCCC‑A‑CCTTCCTGGGGGTGTCAG‑AATTGTTG‑TCTTCCAAGAACCGC‑GGGGGCATAGGGCGAGAGGGCCTTGGCGGCGTTAACTTTAAAATCAGTTAAAACGTTCTGGGCTACCGGGGCGGGTGCCCCAGCCGCGGA‑AAACATCATGCGCATGGCTTCGCGCGGATCGACCGCCAGCATGGCAAACCCGGTCGATGCGCGCGTAATGTCGTTGATGGTGTACTTa              <  2:450303/236‑1 (MQ=255)

                                                                                                                                                                                                                                    caT‑CGCTTTTGGCCC‑A‑ACCCCCGGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAACCGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTattca          <  1:1006400/238‑1 (MQ=255)

                                                                                                                                                                                                                                    aaT‑CGCTTTTTGCCA‑A‑ACCCCCTGGCGGTAACAA‑AATGGTGGATCTACC‑‑GACAGGCGCGAGGCAGAAGGCGAGAGGTCCTTTGGAGGTTTAACGTGGAAATAAGTTAAAACGCTCTCGGTTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATTGCTTCGCGCTTATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTattca          <  2:795325/239‑1 (MQ=255)

                                                                                                                                                                                                                                    aaT‑CGCGTTTTGCCC‑A‑ACCACCTGGCGGTAGCGG‑AATTTTTGATCTACC‑AGAACCGC‑CGGGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTattca          <  1:217344/239‑1 (MQ=255)

                                                                                                                                                                                                                                     aT‑CGGGTTTTGCCA‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTattca          <  1:258959/238‑1 (MQ=255)

ACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGA‑CA‑ATCATGGC‑GCA‑ACTTTTGGCAAT‑CGCGTTTTGCTC‑A‑ACCACCTGGCGGTAGCAG‑AATTGTTGATCTACC‑AGAATCGC‑CGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGC‑AAACATCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTA  >  NC_000913/4072188‑4072658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`