Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	361,668	C→T	16.7%	E65K (GAA→AAA)	lacA ←	thiogalactoside acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	361,668	0	C	T	16.7%	159.2 / 7.3	83	E65K (GAA→AAA)	lacA	thiogalactoside acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (11/57);  new base T (12/2);  total (24/59)
Fisher's exact test for biased strand distribution p-value = 1.08e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCGATCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGT  >  NC_000913/361437‑361888                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      tttcgttCCAGACGTTATTCCCAAGCGGTATCGGAAAAGAGTACATCACGCCG‑TTTGTACTCAATTCAGGGTGTACAGGGGGTCCCGTAACGGAAAGAGTAACGTTGGGGGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑Ag                                                                                                                                                                                                                              <  2:50711/233‑1 (MQ=255) tcccGATCCAGTCTTTATTTCCGTGTGTTAGCGGAAAAGAGTACATCTCGACG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTTCCGTAACGGAAAGAGTATCGTTGGGTGCAATCAGTACGTTATCAGCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑Ag                                                                                                                                                                                                                              <  2:438165/237‑1 (MQ=255)  tCCGATCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGACCCGCAAGAGAAATAGACA‑GGC‑GG‑ATCTA‑ccccag                                                                                                                                                                                                                              >  1:1190141/1‑236 (MQ=255)     gttCCAGATGTTATTCCCAATCTGTATCGGAAAAGGGTCCATCCCGCCG‑TTTTTTCTTAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑t                                                                                                                                                                                                                         <  2:350133/237‑1 (MQ=255)      aTCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGCTGTTGGAACCGAAAGAGAATTAGACA‑GGC‑GG‑GTGTATCCA‑AGGCG‑t                                                                                                                                                                                                                         >  1:89274/1‑239 (MQ=255)      aTCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACA‑GTGTAGTCATCGACAATGGGTAAATTGCAATTTGCATAAAAATTGCGGCCCATTTGGACGTTGGAACCGAAAAATAAATAAAAA‑AGCGGG‑TTTTA‑CCC‑Agcgga                                                                                                                                                                                                                          >  2:1181722/1‑235 (MQ=255)       tCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTCCATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑tt                                                                                                                                                                                                                        <  1:1181722/238‑1 (MQ=255)                 aTTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GGTTTTTA‑CCC‑A‑GCG‑ttttttcctcccc                                                                                                                                                                                                             >  1:252758/1‑230 (MQ=255)                 aTTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGAAGTCATCGACAATGGTTAAATTGAAATTTGCAAAAAAATTCCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAAACA‑GGG‑GG‑GTTTT‑CCC‑AAACG‑ATTTacccataca                                                                                                                                                                                                             >  2:1020599/1‑234 (MQ=255)                                    aaaggttcattctcGCTGTTTTTTTCTCATTTATGGGTGT‑CAGGGTTGCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAAt                                                                                                                                                                                        <  2:217196/229‑1 (MQ=255)                                    aaaGAGTCCATTCAGCCT‑TTTTTTCTCAATTCAGGGTGTGCAAGGTTTCCCGTAACGGAAAGGGTAGCTGTGGGTGCAATCAGTAAGTTATCACCGATTGTGACC‑GTGTAGTCAACGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAAt                                                                                                                                                                                        <  2:156275/239‑1 (MQ=255)                                          tccttctcCCCT‑TTTTTTCTCAATTCATGGTGTAAAGGGTTTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAgg                                                                                                                                                                                    <  2:630975/233‑1 (MQ=255)                                            cATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTTTA‑CCC‑AGGGG‑GGTTTTCCTACCGGGGC‑AAAA‑AATTCtttttttaggggtt                                                                                                                                                                                >  1:89481/1‑228 (MQ=255)                                                        ttttttTCAATTTATGGTGGAGAGGGTGTCCCATTACGGAAAGGGTAACGTTGGGTTCAATTAGTACGTTATCACCGATTGTTTCC‑TTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTc                                                                                                                                                                    <  2:478247/238‑1 (MQ=255)                                                                       ggTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAAAAATAGACA‑GGC‑GG‑ATCTC‑ACC‑AGGGG‑TGTTTTCCTCCCCTGGC‑AAAA‑AAATCCTTTATCCGGCG‑TTCCT‑TTTTTTTCACTTTTtatgggagg                                                                                                                                                    >  2:1308937/1‑235 (MQ=255)                                                                             cAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTc                                                                                                                                                                    <  1:579883/217‑1 (MQ=255)                                                                                tgttccccGTATC‑GAAAGAGTATCGTTTGGGTCAATCTGTTATTTATCCACGATTTTTACC‑TGGTAGGCATCGAAAATGGGTAAATTGAAATTTGCATAAAAATTGGGGCCTATATTGATGTTGGAACCGGAAGGGAAATAGCCA‑GGC‑GG‑TTTTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGAt                                                                                                                                           <  2:599801/231‑1 (MQ=255)                                                                                 ttgtTCCGTAACGGAAAGTGTGACGGTGGGGTCAATAGGTACATGATCACCAATTGTTACC‑GTGTAGTCATCGACAATTGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATTGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTG‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATGTCTTTAATCAGGCT‑TTCTC‑TTATTACAACTTCTGATGG‑A‑TGCGAGTGATTa                                                                                                                                         <  2:888414/237‑1 (MQ=255)                                                                                 gtggCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGAt                                                                                                                                           <  1:850210/236‑1 (MQ=255)                                                                                   gtCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCCAACGGG‑TTTTCCCC‑ACCGCGGC‑AAACAATTTTTTTATACGGCCT‑TTCTT‑TTTTTTCAA‑TTCTTATGG‑T‑TGCAGGGGGTTAAAc                                                                                                                                      >  1:1282188/1‑239 (MQ=255)                                                                                   gtCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑GTCTA‑CCC‑ACGCG‑TTTTCCCCTACCCTGGC‑AAAC‑ATTTCTTTTATTAGGAC‑TTTTC‑TTTTTTTCACTTCTTATGG‑T‑TTCCAGTGGTTtaaa                                                                                                                                      >  1:509319/1‑238 (MQ=255)                                                                                   gtCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑ATTTT‑TCC‑AAGCG‑TTTTTCCCTACCAGGGG‑AAAC‑ATTTTTTTAATTTGTGT‑TTTTCTTTTTTTCACTTTCTTATGG‑A‑TTCTGGTGTTTaaa                                                                                                                                       >  1:740682/1‑239 (MQ=255)                                                                                     cccGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTTTA‑CCC‑CCGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTATACTAGAT‑TTCTC‑TTTTTTTAACTTTTGATGGTA‑TTCCAG‑GATTAAACTc                                                                                                                                    >  1:1051850/1‑239 (MQ=255)                                                                                     cccGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTc                                                                                                                                    <  1:258176/239‑1 (MQ=255)                                                                                     cccGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAAACATAGG‑GG‑TTC‑A‑CCA‑ACGCGTTTTTCCCCTGCC‑TGGC‑AACA‑ATTTCTTTCTTCAAGCA‑TTCTT‑CTTTTTCAACATCTGATGG‑A‑TGCGAGGGGTTAAAATc                                                                                                                                    >  2:405673/1‑239 (MQ=255)                                                                                      ccGTAACGGAAAGAGTAACGTTGGGTGCAATGAGTACGTTATCAACGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCa                                                                                                                                   <  2:143360/239‑1 (MQ=255)                                                                                       cGTAACTGAAAGAGTAACGTTGGGTGCAATCATTTCGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTG‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCa                                                                                                                                   <  1:109690/238‑1 (MQ=255)                                                                                            cGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑GTCTA‑CCC‑CGGCG‑GTTTCCCCTCCCACGGC‑AAAC‑AATTCTTTAATCCGGGT‑TTTTC‑CTTTTTTAAATTCTGTTGG‑A‑TTGGAGGGGTTAAACTCATAAAtt                                                                                                                             >  1:697277/1‑239 (MQ=255)                                                                                              gAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑a                                                                                                                                                                                                  <  1:765677/172‑1 (MQ=255)                                                                                                   agTATCGTTGGGTTCAATTCTTTAGTTATCCGCTATTTTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTa                                                                                                                            <  2:510360/233‑1 (MQ=255)                                                                                                           tGGGTGCAATCAGTACCTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGa                                                                                                              <  1:405673/239‑1 (MQ=255)                                                                                                             ggTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTT‑CCC‑AGGCG‑GTTTTCCCTACCGTGGC‑AAAC‑ATTTTTTTTATTAGGCT‑TTTTC‑TTTTTTTTAATTTTTATTG‑A‑TGCGGGGGGTTAAAATCCAACATTTACGTTTTtcccccg                                                                                                              >  1:510360/1‑233 (MQ=255)                                                                                                                    tCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCttttt                                                                                                     <  1:500303/239‑1 (MQ=255)                                                                                                                    tCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCttttt                                                                                                     <  1:1308937/239‑1 (MQ=255)                                                                                                                           gTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTa                                                                                              <  1:62044/239‑1 (MQ=255)                                                                                                                             ttaccccGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTa                                                                                              <  1:1022014/232‑1 (MQ=255)                                                                                                                              aTCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTTTA‑CCC‑CGGCG‑TTTTTCCCCACCGTGGC‑CAAA‑ATTTCTTTTATAAGGCT‑TTTTC‑TTTTTTCAACTTCTGATTG‑A‑TGTCAGTGATTAAACTCCCACATTAAAGTTTTTCCCCCAAAGCTTTTTTCTCGTAAg                                                                                            >  1:433470/1‑238 (MQ=255)                                                                                                                              aTCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTTTA‑CCC‑CGGCG‑GGTTTCCCCCCCGTGGC‑AAAC‑AATTCTTTTATTAAGGC‑TTCTC‑TTTTTTTTAATTTTTATGG‑A‑TTCGGGTGGTTAAAATCATACATTTACGTTTTTTCCCGAAATCTTTTTTTCGGTAAg                                                                                            >  1:453328/1‑238 (MQ=255)                                                                                                                              aTCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AAGCG‑TTTTCCCCTCCCCTGGC‑CAAA‑ACTTCTTTTATCTGGCT‑TTCTC‑TTTTTTTACCTTCTTTTGT‑G‑TGCGCGTGGTTTAAATCTTACAATTACACTTTTCCCCCAAATCTTTTTTTCGGTaat                                                                                            >  2:239760/1‑237 (MQ=255)                                                                                                                                  ccGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAGTTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTT‑CCC‑TCGCG‑GTTTCTCCCTCAAGGGC‑AAAC‑AAATCTTTTATCCGGCTCTGTTT‑TTTTTTAAACTTCT‑ATGG‑ATTTCGA‑TGATTAAACTACTAAATTAAATTTTTGCCCCCAAAGATTTTTTCTGGGAAGCCTTc                                                                                       >  2:936339/1‑239 (MQ=255)                                                                                                                                  ccGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTTTA‑CCC‑AGGGG‑TTTTTCCCTACCACGGCAAAAC‑ATTT‑TTTTATAAGGCT‑TTTTC‑TTTTTTCTAACTCTTATTG‑A‑TTGCAGGGGTTAACATCATACAATTACGTTTTTCCCCCAAACTTTTTTTCTGGGTAACCTTc                                                                                       >  1:232272/1‑239 (MQ=255)                                                                                                                                  ccGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑CGGCG‑GTTTTTCCCACCCTGGC‑AAAA‑CATTATTTATTTCGGCT‑TTTGT‑TTTTTTTTACTTCTTCTGG‑T‑TGCGAGTGGTTATACTCATAAATTTACATTTTCTCCCCCAATCTTTTTTTTCGTAAGACTTc                                                                                       >  2:1091272/1‑239 (MQ=255)                                                                                                                                   ctaTTGTTACC‑GTGTATTCATCGACAATGGTTAAATTGAAATTTGCATAAAAAGTGCGGCCTATATGGATGTTTGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCAA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCg                                                                                      <  1:1236067/237‑1 (MQ=255)                                                                                                                                     gttttttcc‑GTTTTGTCAACGACTATGGTTAAATTGAAAATTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGca                                                                                    <  2:509319/232‑1 (MQ=255)                                                                                                                                     aTTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGa                                                                                                              <  1:156754/213‑1 (MQ=255)                                                                                                                                       ttttACC‑GTGTATTCATCGAAAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGcaca                                                                                  <  2:1282188/237‑1 (MQ=255)                                                                                                                                       tGTTACCGGTGT‑GTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGcaca                                                                                  <  2:138559/239‑1 (MQ=255)                                                                                                                                       tGTTACC‑GTTTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGcaca                                                                                  <  1:283902/239‑1 (MQ=255)                                                                                                                                       tGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTTTA‑ACC‑CGGGG‑TTTTTCCCTACCCGGGC‑AAAC‑AATTCTTTTATAAGGCT‑TTTTT‑CTTTTTTAACTTCTGATGG‑G‑TGCCGGTGATTAAACTCATAACTTAACGTTTTTCCCCCAAGTCTTTTTTTCGGTAAGCCTTctcccc                                                                                  >  1:138559/1‑234 (MQ=255)                                                                                                                                       tGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGcaca                                                                                  <  1:887237/239‑1 (MQ=255)                                                                                                                                            cc‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTa                                                                                              <  1:1082459/222‑1 (MQ=255)                                                                                                                                                          gACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGc                                                                <  1:1242908/239‑1 (MQ=255)                                                                                                                                                           aCAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                               <  1:372979/239‑1 (MQ=255)                                                                                                                                                            cAATTGTTAAATTTAAATTTGCATAAAAATTGCGGCCTATAGTGGTGTTGGACCCGTAAGATAAATAGACA‑TGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATGTCGTTAATGAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                               <  2:1326882/238‑1 (MQ=255)                                                                                                                                                            cAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTc                                                                                                                                                                    <  1:1155842/139‑1 (MQ=255)                                                                                                                                                            cAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                               <  1:748180/238‑1 (MQ=255)                                                                                                                                                            aaaTGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑TG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                               <  1:423630/237‑1 (MQ=255)                                                                                                                                                            aaaTGGTTAAAGTGAAATTGGCAAAAAAATTGCGGTCTATATGGATGTTGGAACCGGAAGAGAAATAGACA‑GGG‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTGCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGcc                                                               <  2:713353/237‑1 (MQ=255)                                                                                                                                                                   tAAATTGAAATTTGCGTAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTa                                                       <  1:222481/239‑1 (MQ=255)                                                                                                                                                                    aaaTTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑TGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTAt                                                      <  1:708299/239‑1 (MQ=255)                                                                                                                                                                           aaTTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑CGGCG‑TTTTTCCCTACCCTGGC‑AAAC‑ATTTCTTTTATACGGCT‑TTTTC‑TTTTTTTAACTTCTTGTGG‑G‑TTCGAGGGATTTAAATCATACATTAACGTTTTCTCCCGCAGGCTTTTTTTCCGTAAAGCTTTCCCCATATTGGGAAAAAGCTTGCCTGCTCTtttttttttg                                               >  1:264262/1‑237 (MQ=255)                                                                                                                                                                                   tAAAAATTGCGGCCTATATGGTGGTTGGACCCTTAAGAGAAATAGACA‑GGC‑GG‑TTCTT‑CCC‑GCGCC‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTgg                                       <  2:89481/239‑1 (MQ=255)                                                                                                                                                                                   tAAAAATTGCGGCCTATATGGATTGTGGAACCGTAAGAGAAATAGGCA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTgg                                       <  1:543799/239‑1 (MQ=255)                                                                                                                                                                                    aaaaaTTGCGGCTTATATGGATGTTGGACCCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTG‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGc                                      <  1:936339/239‑1 (MQ=255)                                                                                                                                                                                    aaaaaTTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGc                                      <  1:472638/239‑1 (MQ=255)                                                                                                                                                                                                    ttGGGTGTTGGGACCGTAAGGGAAATAGACC‑GGC‑TT‑TTCTA‑CCC‑CGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCa                      <  2:428975/238‑1 (MQ=255)                                                                                                                                                                                                    ttGGATTTTGGAACCGTAAGATAAAAAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTGTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCa                      <  2:1051850/238‑1 (MQ=255)                                                                                                                                                                                                    tgggATGTTGGAACCGTAAGAGAAATAGACC‑GGC‑GG‑TTCTT‑CCC‑AGGCT‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCGC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCa                      <  1:1192251/237‑1 (MQ=255)                                                                                                                                                                                                    atggatggTGGAACCGTAAGAGAAATAGACA‑GGC‑CG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCa                      <  1:1091272/239‑1 (MQ=255)                                                                                                                                                                                                    atGGATTTTGGAACCGTAAGAGAAATAGACG‑GGC‑GG‑TTCTA‑CCC‑AGTCG‑TTTTCCCCAGCCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCATCTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCa                      <  2:264262/239‑1 (MQ=255)                                                                                                                                                                                                    atGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCCA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCa                      <  1:540775/239‑1 (MQ=255)                                                                                                                                                                                                      ggATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt                    <  1:735626/239‑1 (MQ=255)                                                                                                                                                                                                      ggAGGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt                    <  1:877815/239‑1 (MQ=255)                                                                                                                                                                                                         tGTTGGACTCGTTAGGGAAATTGAGC‑TGC‑TG‑TTCTA‑CCT‑CGGCG‑TTTTCCCCTACCGTGGC‑AAAA‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TTCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg                 <  2:740682/239‑1 (MQ=255)                                                                                                                                                                                                         tGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg                 <  1:184057/239‑1 (MQ=255)                                                                                                                                                                                                         tGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg                 <  1:1193350/239‑1 (MQ=255)                                                                                                                                                                                                          ttgtgAACCGGAAGAGAAAAAGACA‑GTG‑GG‑GTCGT‑CCC‑CGGCG‑TTTTCCCCATCCGTGGC‑AAAC‑ATGTCTTTAATCAGGAT‑TTTCC‑TTTTTCTAACGGTTGATGG‑T‑TTCGAGTGATTAAACTCCTACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTAGTCTTGCGGTCATTGGCATGTTCAATTCGAGCCCTCCg                 <  2:1068624/234‑1 (MQ=255)                                                                                                                                                                                                          gTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg                 <  1:911596/238‑1 (MQ=255)                                                                                                                                                                                                          gTTGGAACCGTAAGAGAAATAGACA‑GGC‑CG‑TTCTT‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg                 <  1:252310/238‑1 (MQ=255)                                                                                                                                                                                                           ttGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTTTA‑CCC‑AGGCG‑TTTTTCCCTACCGTGGC‑AAAC‑ATTTTTTTAATCAGGCT‑TTTTC‑TTTTTTTAAATTCTGATGG‑A‑TGCGAGGGATTAAACTCATACATTTAAGTTTTTCCACCAAGGCTTTTTTTCGGTAAGCCTTCCCACATATTGGTAAAAAGATTGCCTCCTCCTATTTTTTTGGTCATTTGCATGTTCAATTCGATCACTCCCtt               >  1:931024/1‑239 (MQ=255)                                                                                                                                                                                                             ggAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTTTA‑CCC‑CGGCG‑TTTTTCCCCACCGTGGC‑CAAA‑AATTCTTTTATCAGGCT‑TTCTC‑TTTTTTTAACATCTTGTTG‑G‑TGCGCGTGGTTAAACTCCTACATTTAAGTTTTTCCCCGAAGTCTTTTTTCCCGTAAGCCTTTGCACACATCCGTGAAAAGCTTTCCTTCTCTTATTTTTTTGGTCATTGGCATGTTTAATTCGAGAACTCCCtttt             >  1:1294466/1‑237 (MQ=255)                                                                                                                                                                                                                        agaAATAGGAA‑TGC‑GG‑TGTTT‑CCC‑CGGTT‑TTTTCCCCTACCGTGGC‑AAGC‑AGTTTTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTAATTAAACTCATACATTAAGGTTTTCCCACGAAGTCTTTTTGCCGGTAAGCCTTCGCTCGTATCGGTAAATAGCTTGCATGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGt  <  2:1190141/239‑1 (MQ=255)                                                                                                                                                                                                                                aCA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg                 <  1:98618/216‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      TTCCGATCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCG‑TTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACC‑GTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACA‑GGC‑GG‑TTCTA‑CCC‑AGGCG‑TTTTCCCCTACCGTGGC‑AAAC‑ATTTCTTTAATCAGGCT‑TTCTC‑TTTTTTCAACTTCTGATGG‑A‑TGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGT  >  NC_000913/361437‑361888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑