BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,101,255	C→T	9.6%	S149F (TCT→TTT)	sodA →	superoxide dismutase, Mn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,101,255	0	C	T	9.6%	246.1 / 3.0	103	S149F (TCT→TTT)	sodA	superoxide dismutase, Mn
Reads supporting (aligned to +/- strand): ref base C (23/68); new base T (7/4); total (30/73)
Fisher's exact test for biased strand distribution p-value = 1.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CGTACTGCGCAACAACGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACC  >  NC_000913/4101019‑4101489

cGTACTGCGCAACAACGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATtctc                                                                                                                                                                                                                                                <  1:303568/239‑1 (MQ=255)

  tACTGCGCAACAACGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGGGGGTTTTACTGGTAACCCGGATTCTccc                                                                                                                                                                                                                                              >  1:388361/1‑238 (MQ=255)

          aacaacGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGGCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGGGGGTTTTAATGCTTACCAGGGTTTTcccccgtgggg                                                                                                                                                                                                                                      >  1:823344/1‑230 (MQ=255)

               cGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGGGGTGTTTACTTCTAACCCGGAGTTTCCGCTGGTGGTGGAAg                                                                                                                                                                                                                                 >  1:478991/1‑239 (MQ=255)

                gCTGGCGGTCACGCTACCCACAGACTGTTCTGGAAAGGTCTGAAAAAAGGCCCCACCCTGCAGGTGGACCTGAAAGCGGCGTGCGAACGTGACTTCGGTTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGc                                                                                                                                                                                                                                <  2:326555/239‑1 (MQ=255)

                              aaaCCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCCCCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCtt                                                                                                                                                                                                                <  1:1005494/238‑1 (MQ=255)

                                      gCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTAATGCTAACCAGGGTTCTCCGCTGCTGGGTGAAGC‑T‑ATTTCTGGCCGTTC‑CGGCtt                                                                                                                                                                                                        >  1:466402/1‑238 (MQ=255)

                                       ccTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGTCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAAGTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTcc                                                                                                                                                                                                      <  1:412933/239‑1 (MQ=255)

                                        cTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCt                                                                                                                                                                                                         <  1:672003/235‑1 (MQ=255)

                                          gTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGGTCAACTGGCGGGGGTTTTTCTCGCTAACCCGGATTTTCCCCCGTTGGGGGAGGG‑T‑AATTTTGTGGCTTC‑CGCCTTccccg                                                                                                                                                                                                   >  2:1010784/1‑237 (MQ=255)

                                           ttCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCt                                                                                                                                                                                                                       <  1:54550/219‑1 (MQ=255)

                                            ttgggAAAGGTTTTAAAAAAGCCCGCACCCTGCAGGGGGACCTGAAAGCGGCTATCGAACGTGACGTCGGCTCCGTTGATAGCTACAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGAtt                                                                                                                                                                                                 <  2:1327169/236‑1 (MQ=255)

                                            tCTGGAAAGGTCTGAAAAAAGGCCCCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGAtt                                                                                                                                                                                                 <  1:1125550/239‑1 (MQ=255)

                                              tGGAAAGGTCTGAAAAAAGGCCCCCCCCGGCAGGGTGACCTGAAAGCGGCTATCGAACGTGAATTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTAt                                                                                                                                                                                               <  1:345797/239‑1 (MQ=255)

                                              tGGAAAGGTCTGAAAAAAGGCCCCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTAt                                                                                                                                                                                               <  1:303156/239‑1 (MQ=255)

                                              tGGAAAGGTCTGAAAAAAGGCACCCCCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTAt                                                                                                                                                                                               <  1:172283/239‑1 (MQ=255)

                                              gtgAAAGTGCTGAAAAAAGTCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACTTGACTGCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTAt                                                                                                                                                                                               <  2:264757/237‑1 (MQ=255)

                                              gtgAAAGGTCTGAAAAAAGGCACCCCCCTGCAGGGTTACCTGAAAGGGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGGAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTAt                                                                                                                                                                                               <  2:67190/237‑1 (MQ=255)

                                                 aaaGGTCTGAAAAAAGGCCCCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATgg                                                                                                                                                                                             <  1:691463/238‑1 (MQ=255)

                                                 aaaGGTCTGAAAAAAGGCACCCCCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATgg                                                                                                                                                                                             <  1:626539/238‑1 (MQ=255)

                                                     gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGCAAGGCGATAAACTGGCGGTGGATTCTACTGCTTAACAGGATTTTCCTCTGATGGGGGAAGC‑T‑ATTTCTTGCCCTTC‑CGGCGTCCCGATTATTGGACTg                                                                                                                                                                                        >  1:1327169/1‑239 (MQ=255)

                                                     gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTTTCCTGCTAACCCGGATTTTCCGCTGATCGGGGAAGG‑T‑ATTTTTGGGGATTC‑CCGGTTCCCGATTATGGGCCTg                                                                                                                                                                                        >  1:30525/1‑239 (MQ=255)

                                                     gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCCAACCCGGATTCTCCGCTGATGGGGGTAGG‑C‑ATTTCTGGGCGTTC‑CGCCTTCCCGATTTTGGGGCTg                                                                                                                                                                                        >  1:1112239/1‑239 (MQ=255)

                                                     gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGGTTATGGGCCTg                                                                                                                                                                                        >  1:1166107/1‑239 (MQ=255)

                                                     gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACCAGGATTCTCCGCTGATGGGGGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTg                                                                                                                                                                                        >  1:193403/1‑239 (MQ=255)

                                                     gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGGGGTTTTTCACGCTAACCCAGGTTCTCACCTGGTGGGGGGGAC‑T‑ATTTTTTGTGCTTC‑CGGCTTTCCCACTATggggcgg                                                                                                                                                                                        >  2:1054157/1‑235 (MQ=255)

                                                       ttGAAAAAAGGCCCCCCCCAGTGGGGGGGCCTGAAAGTGGCTTTCGAACGGGACTTCGGCTCCGGTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCATGTTCCCTGTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTgg                                                                                                                                                                                       <  2:707839/237‑1 (MQ=255)

                                                       gtGAAAAAAGGCATCAACCGGCAGGGTTAACTAAAAGGGGTTTAAGAACGTGACGTGTGCTCCGTTGATAACTTCGAGGCGGCATTTGAAAAGGCGGCAGGTGCACGATTTGGTGCCGGTTGGTGA‑TGGCTGGTGCTGAAAGGCGATAAGCTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGATGC‑T‑ATTTCTGGCGCTTG‑CGGCTTACGGATTATGGGCCGGGa                                                                                                                                                                                      <  2:476476/238‑1 (MQ=255)

                                                       cTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTgg                                                                                                                                                                                       <  1:327835/238‑1 (MQ=255)

                                                       cTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTAACGAACGTGACTTCGGCTCCGTTGATAACTGCAAAGCAGGATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGa                                                                                                                                                                                      <  1:49551/239‑1 (MQ=255)

                                                        ttaaaaaaTGCCCCTCCCTGCAGGGGTACCGTAAAGGGGCTTTCGACAGTGCCTTCGGTTCCGTTGATAACTTCAAAGCAGAATTTGACAAAGCGGCAGATGCCCGCTTTGGTTCCGGCTGGTCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGAt                                                                                                                                                                                     <  2:222054/237‑1 (MQ=255)

                                                          aaaaaaGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTTTACTGCTAACCAGGGTTCTcc                                                                                                                                                                                                                                               >  1:993410/1‑182 (MQ=255)

                                                           aaaaaGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGAtgtg                                                                                                                                                                                  <  1:174591/239‑1 (MQ=255)

                                                                  caccacCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGaa                                                                                                                                                                           <  1:413429/238‑1 (MQ=255)

                                                                   cccccccTGCGGGGTGTCCGGAAACGGGTTATGGAAATGTACTTCGGTCCCGTTGATAACTTCAAAGGAGAAGTTGAAAAAGCGGCAGCTTCCCGGTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACa                                                                                                                                                                         <  2:388361/235‑1 (MQ=255)

                                                                   accaccCTGCAGGGGTACCTGAAAGGGGGTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAAc                                                                                                                                                                          <  1:629723/238‑1 (MQ=255)

                                                                         cTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTta                                                                                                                                                                  <  1:1099863/239‑1 (MQ=255)

                                                                           gCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTTTACTGCTAACCAGGATTCTCCGCTGTTGGGGGAAGC‑T‑ATTTTTTGGGCTTC‑CGGCTTCCCCCGTATTGGCCTGGGTGTGT‑GGGACCA‑TGCTtaat                                                                                                                                                                >  1:138582/1‑237 (MQ=255)

                                                                            cAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAAACAGGGTTCTCCGCCGATGGGTGAAGC‑C‑ATTTCTGGCGCTTC‑CGGCTTCCCCATTATGGGCCTTGCAGTGT‑GGGAAAC‑AGCTtaca                                                                                                                                                                >  1:705635/1‑237 (MQ=255)

                                                                                 tGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTTTACTGCCTAACAGGATTCTCCCCTGATTGGGGAAAC‑TCTTTTTTGGGGTTCC‑CCGTTTCCCGATTTGTGGCCTGGA‑GTGT‑GGGAAAC‑TGCTTTCTAcccgc                                                                                                                                                          >  2:1078584/1‑236 (MQ=255)

                                                                                  gACTTGAAAGCGGCTCACGAAGGTGTCTTCGGCTCCGTTGATAACTAAAAAGCGGAATGTGAAAAAGCGGCAGCTTCCGGCTTGGGTTGCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTACTTGCGCTTC‑CGGCTTCACGATTATGGGCCTGGATGTGT‑GGGAAAA‑TGCTTACGACATGa                                                                                                                                                          <  2:321931/238‑1 (MQ=255)

                                                                                  gACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCCACTGGTCACCCGGAATCTCCGCTGATGGTGGGAGCAA‑ATTTATGG‑GCTTCTCGCCCTCCCG‑TTATGGGGCCGGACGGGT‑GGGGCAA‑TGCTTAATAccctaa                                                                                                                                                         >  2:278529/1‑235 (MQ=255)

                                                                                              gCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGGGAAGC‑T‑ATTTCTGGCGCTTC‑CCGCTTCCCGATTATGGGCCCGGATGTGT‑GGGGACA‑TGCTTTATACCTGAAATTTCAGAAcc                                                                                                                                              >  1:1027496/1‑238 (MQ=255)

                                                                                               cTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAAccg                                                                                                                                             <  1:823268/238‑1 (MQ=255)

                                                                                               cTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAAccg                                                                                                                                             <  1:1126010/238‑1 (MQ=255)

                                                                                                       ctttccTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGGGGCAGTTTCCCGCTTTGGTTCCGGCTGGGCA‑GGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                    <  2:1027496/234‑1 (MQ=255)

                                                                                                       cgggACTTCGGCTCCGTTGATACCTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                    <  1:737509/236‑1 (MQ=255)

                                                                                                       cctgccTTCCGCTCGGTTGATACCTTCAAACCAGAATTTGAAAACGCGGCAGTTTCCGGCTTTGGTGCCGGCTGGGCA‑GGGCTGGTGCTGAAAGGCGATAACCTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTCCCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                    <  1:239313/234‑1 (MQ=255)

                                                                                                       cGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                    <  1:1010784/239‑1 (MQ=255)

                                                                                                       cGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGGGGGTTTTACTGCTTACCAGGGTTTTCCCCGGATGGGTGAAGC‑C‑TTTTTTGGCGGTTC‑CGGCTTCCCCATTATGGGCCCGGGTGTGT‑GGGGAAA‑TGCTTTCTACCCGAAAATCCAGAACCCCCGCCCggc                                                                                                                                    >  2:823268/1‑238 (MQ=255)

                                                                                                       agTGACTCCGACTCCGTTGATAACTTCAAAGCAGAATGTGAAAAAGCGGCAGCTCCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                    <  1:890649/238‑1 (MQ=255)

                                                                                                         tGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                    <  1:436167/237‑1 (MQ=255)

                                                                                                         tGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCCTCTGCTAACCAGGGTTCTCCGCGGATTGGGGGAGG‑C‑AGTTTTGTGGCCTC‑CCGCCTCCCCGATATGGGGCTGGGGGTGT‑GGGACAC‑TGGCTACTACCTCACATCCCAAAAacgcacgcccgact                                                                                                                                  >  2:447850/1‑229 (MQ=255)

                                                                                                          tgtttCGGGTCCGTTAGTAACTTAAAAGGAGAATTTGAAAAAGGGGCAGCTTCCCGCTTTGTGTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGTTTTCTACTGCTAACCAGGTTTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTa                                                                                                                                 <  2:1090356/236‑1 (MQ=255)

                                                                                                               cGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTa                                                                                                                            <  1:267394/239‑1 (MQ=255)

                                                                                                               aggCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTa                                                                                                                            <  1:680889/238‑1 (MQ=255)

                                                                                                                 gCTCCTTTGGTTATTGCAAAGCGGATTTTGAAAAAGCGGGTGCTTCCCGTTTTGGGTCCGGTTGGGAC‑TGGGTGGTGGTGAAAGGCGATAAACTGGCGGTGGTTTCGACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTCCCTGAAATGCCAGAACCGCCGTCCGGACTACATTaaa                                                                                                                          <  2:1302140/239‑1 (MQ=255)

                                                                                                                  ctacGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTaaa                                                                                                                          <  1:1078584/235‑1 (MQ=255)

                                                                                                                  cTCCGTTTATAACTTCAAAGCAGAATTTGAAAATGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGTCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTaaa                                                                                                                          <  1:734541/238‑1 (MQ=255)

                                                                                                                  cTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTaaa                                                                                                                          <  1:1054157/238‑1 (MQ=255)

                                                                                                                   tCCGTTGATAACTTCAAAGCAGAATTGGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAaga                                                                                                                        <  1:54152/239‑1 (MQ=255)

                                                                                                                      ggtGATAACTTCAAAGCAGAATTTGAAAAAGGTGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGtt                                                                                                                     <  1:807130/237‑1 (MQ=255)

                                                                                                                       ttGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGa                                                                                                                                                          <  1:1202392/201‑1 (MQ=255)

                                                                                                                               ttCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                    <  1:312434/215‑1 (MQ=255)

                                                                                                                                tCAAAGGAGATTTGGAAAAACCGGGAGCTTCCCGCTTTGGGTCCGTCTGGGCA‑TGGCTGGTGCTTAAAGGCGATAAACTGGCGGTGGTTTCTCCTGCTAACCAGGATTCTCCGCTGAGGGGTGAAGC‑T‑AGTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGGGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACgtg                                                                                                           <  2:478991/239‑1 (MQ=255)

                                                                                                                                tCAAAGCAGAATTTGAAAAAGCGGCATCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTCCTACTGCTAACCAGGATTCCCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACgtg                                                                                                           <  1:15076/239‑1 (MQ=255)

                                                                                                                                 cAAAGCAGAATTTGAAAAAGCGGAAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGTTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACgtgg                                                                                                          <  1:1109391/239‑1 (MQ=255)

                                                                                                                                      caaaaTTTGAAAAACCGGCACGTTCCCGGTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTTAAAGGCGATAACCTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAAc                                                                                                     <  1:835992/236‑1 (MQ=255)

                                                                                                                                       aGAATTTGAAAAAGCGGCAGCTTCCCTGTTTGGGTGCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCTATAAACTGGCGGTGTGTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAAc                                                                                                     <  2:193403/238‑1 (MQ=255)

                                                                                                                                         aaTTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATTGGTGAAGC‑T‑ATTTTTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGGAAA‑TGCTTACTAACTGAAATTCCCGAACCGCCGTCCGGGCTACATTAAAGAGTTCTTGAACGTGGGGAAATgg                                                                                                  >  1:292746/1‑239 (MQ=255)

                                                                                                                                          aTTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAAc                                                                                                     <  1:586678/235‑1 (MQ=255)

                                                                                                                                           tttGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑ggg                                                                                                                                                                             <  1:1123609/163‑1 (MQ=255)

                                                                                                                                                aaaacgtgatccTTCCCGGTTTGG‑TCCGGGTGGGTACTGGTCGGTGTTGAAAGGTGTATACCGGGCGGTGTTTTTGCCTTCCAACCGGGAGTCACCGCTGATGGGGTGTGC‑T‑ATTTCTGGCCCTTC‑CGGCTCCCCAATTAGGGGCCTGGATGTTT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAag                                                                                           <  2:1112239/228‑1 (MQ=255)

                                                                                                                                                aaaaGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTTTACTTCTAACAAGGAGTTTCCGCTTATGGGGGGAGG‑C‑ATTTCTCGCGCTTT‑CTGGCTCCCGATTATGGGGCTGGGTGGGT‑GGGGACACTGC‑TAATACCCGGAATTCAAAAAACCCCCCCGGGCCCACATTAAACAGGTTTGGAACGTGGGGaaacggggggaac                                                                                           >  2:1027214/1‑228 (MQ=255)

                                                                                                                                                                     tGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGa                                                                      <  1:1251236/239‑1 (MQ=255)

                                                                                                                                                                     tGGTTCAGGCTGGGCC‑TGGTTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGTATTCCCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGa                                                                      <  1:1271937/239‑1 (MQ=255)

                                                                                                                                                                               gtgggtcttgCTGGGGTTAAAAGGCGATATGCTGGGGGGTGTTTCTACTGCTAACCAGGATCTTCCGCTGAGGGGGGAAGC‑T‑ATTTCGGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGAAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAaataat                                                             <  2:548369/230‑1 (MQ=255)

                                                                                                                                                                                       ggCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTa                                                                                                                                                                                                >  2:186395/1‑102 (MQ=255)

                                                                                                                                                                                        gCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACgtg                                                                                                           <  1:302484/184‑1 (MQ=255)

                                                                                                                                                                                          ttgtggtgAAGGGCGATAAACTGGCGGTTTTTTCTACTGCTAACCAGGATTCTCCGCTTGTGGGGGAACG‑T‑ATTTTTGGCGCTTC‑CGGCGTCCCGATTAGGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTgccgcc                                                  <  2:823344/237‑1 (MQ=255)

                                                                                                                                                                                          ttgTGCTGAAAGGCGTTAATCTGGGGGTTGTTTCTACAGTTACCCAGGTTTCTCCGCGGATGGGGGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTCTGGGCCTGGATGTGG‑GGGAACA‑TGCTTACTACCTGAAATTCCAGATCCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCTAAAAAATAATCATTTgccgc                                                   <  2:1077524/236‑1 (MQ=255)

                                                                                                                                                                                             ggCTGAAAGGCGAAAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCctg                                                <  1:1014667/237‑1 (MQ=255)

                                                                                                                                                                                                 gAAAGGCTATATATGGGCGGGTGTTTGTTCTGCTAAGCAGGATTTCCCGTGGGTGGTGGAAGC‑T‑TTTTCTGGCGTTTC‑CGGCTTCCAGATTAGGGGCTGGGATGTGT‑GGGAACA‑TGCTTCCTACCTGAAATTCCAGGACCGGCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCa                                           <  2:324349/239‑1 (MQ=255)

                                                                                                                                                                                                   aaGGGGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAt                                         <  1:923519/239‑1 (MQ=255)

                                                                                                                                                                                                   aaGGCAATAATCGTGGGGTTGGTTCTACCGGTAAGCAGGGTTGACCGTTGATGGGTGAACG‑T‑TTGTGTGGCGCTTC‑GGTCTGCCCGCTTAGGGGCCGGGA‑GTGTGGGGAACA‑TGATTACTACCAGAAGTGCCATGTCGGCCGTCCTGACTACATTAAATAGTTCTGGAACGTGGTGAACTGGGACTAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTGGCCGCCTGCTGCAAt                                         <  2:63309/239‑1 (MQ=255)

                                                                                                                                                                                                       cggtAAGCTGGGGGTTGTTTCTCCTTCGAAACGGGATTTACCGCTGATGGGTGAGGT‑T‑TGTTCGGGCGCTGT‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑Ga                                      <  2:724328/234‑1 (MQ=255)

                                                                                                                                                                                                       cGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAAACGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAgg                                    >  1:251227/1‑239 (MQ=255)

                                                                                                                                                                                                       cGATAAACTGGCGGTGGGTTCTACTGCTAACCAGGATTCTCCGCTGATGGGGGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGCTTATGGGCCTGGAAGTGT‑GGGGAAA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGGCTACATTTAAGAGTTCTGGAACCGGGAGAACTGGGGCGGAACAGCGGCCCGGTTTTCGGCGAAAAAAAAAAAATTTTCCCCCCGATGCAAtaggg                                     >  2:79982/1‑235 (MQ=255)

                                                                                                                                                                                                           aaaCTGGCGGTGGTTTCTACTGCAAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGta                                <  1:941266/239‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGGGGTGGTTTCTACCGCTAACCAGGATTCTCCGCGGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGtata                              <  1:1141172/239‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGCGGTGGTTTCTACTGCTAACCGGGATTCCCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCCCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGtata                              <  1:1083497/239‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGCGGTGGTTTCTACTGCTAACCAGGGTTCTCCGCTTATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGtata                              <  1:499817/239‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGtata                              <  1:425717/239‑1 (MQ=255)

                                                                                                                                                                                                                      ggtTTCTACTGCTAACCAGGATTCTCCGTTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGTATAGGCCGCata                     <  1:359100/239‑1 (MQ=255)

                                                                                                                                                                                                                              cTGCTAACCAGGATATCCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCATGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGTATAGGCCGCATATCAGCTTa             <  1:522015/239‑1 (MQ=255)

                                                                                                                                                                                                                                 cTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGTATAGGCCGCATATCAGCTTaaaa          >  1:1109519/1‑239 (MQ=255)

                                                                                                                                                                                                                                 cTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGAGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATAATTTGCCGCCCGCTGCAAT‑GAGGCGTAAAGGCCGCATACAAGTTTaaaa          >  2:1194585/1‑239 (MQ=255)

                                                                                                                                                                                                                                    aCCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTCCAAT‑GAGGCGTATAGGACGCAAAAAAACTTAAAAAAt       >  2:17702/1‑239 (MQ=255)

                                                                                                                                                                                                                                        ggATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGGTTTACGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGTATAGGCGGCATATCAGCTTAAAAAATGaa    >  1:1012284/1‑238 (MQ=255)

                                                                                                                                                                                                                                         gATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGACCA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTCAAGAATTCTGGAACGTGGTGAACTGGGTCGAAGAACCGGCACGTTTTGCGGTGAAAAATTAATCATTTGCCGCCCCCTGCAATGGAGCGGTAT‑GGCCGCAAATAAACATAAAAAAAGGAcc  >  2:1261818/1‑239 (MQ=255)

CGTACTGCGCAACAACGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCA‑TGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGC‑T‑ATTTCTGGCGCTTC‑CGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACA‑TGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAT‑GAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACC  >  NC_000913/4101019‑4101489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`