Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,830 T→G 18.8% V109G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,8300TG18.8% 214.0 / 8.2 108V109G (GTG→GGG) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (23/57);  new base G (18/7);  total (44/64)
Fisher's exact test for biased strand distribution p-value = 2.84e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTT  >  NC_000913/429601‑430066
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             
ctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGCAACCGGCGGTCAGGTGGTTGGcg                                                                                                                                                                                                                                       >  1:920530/1‑237 (MQ=255)
ctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGcg                                                                                                                                                                                                                                       >  1:1106067/1‑237 (MQ=255)
ctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCa                                                                                                                                                                                                                                       >  1:219780/1‑238 (MQ=255)
ctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGAAGCCATGACATCATGGTCCGGATGCCGCCTGCTGAAGGCGAAACCGGCGGGCCGGGGTTGGGcc                                                                                                                                                                                                                                       >  2:1240085/1‑237 (MQ=255)
 tgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGGGAAACCGTCGGCATGGGGTGGGGCAg                                                                                                                                                                                                                                      >  1:1049081/1‑238 (MQ=255)
   tttaTCGCTTTTTTCGTTATTATGGGCTTGGGGGGTTTTAACTGGGGGCTGGATTTCACGGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGAAAAACGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCa                                                                                                                                                                                                                                   <  2:1313667/236‑1 (MQ=255)
     aaTCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGGATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAgg                                                                                                                                                                                                                                 >  1:219774/1‑239 (MQ=255)
          ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGCCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGCGTTGGGCAGCCGGGGTCTg                                                                                                                                                                                                                            >  1:474810/1‑239 (MQ=255)
          ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTg                                                                                                                                                                                                                            >  1:677038/1‑239 (MQ=255)
          ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCAGGGGTTGGGCAGCCAGGTTCTg                                                                                                                                                                                                                            >  1:266733/1‑239 (MQ=255)
          ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAAATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTTGGCAGCCAGGTTCTg                                                                                                                                                                                                                            >  1:155323/1‑239 (MQ=255)
           ttctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGa                                                                                                                                                                                                                           <  1:264498/237‑1 (MQ=255)
           tgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGCAATCATGGTCCGAATGCCGCCTGCTGAAGGCGAAACCGGCGGCAAGGGGTTGGGCACCCAGGGTCTGa                                                                                                                                                                                                                           >  1:752079/1‑239 (MQ=255)
            ggtATCGTTATTGTGGGCGGTGCGGGTTTTAACTGGGGGCTTGATTTCCCCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGaa                                                                                                                                                                                                                          <  1:1317363/237‑1 (MQ=255)
             ctATCGTTCTTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGAGGCCAGGGTCTGAAg                                                                                                                                                                                                                         >  1:423754/1‑239 (MQ=255)
             ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGTGTGGGCAGCCAGGGTCTGAAg                                                                                                                                                                                                                         >  1:578585/1‑239 (MQ=255)
             ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGGCAGGGGTTGGGCAGCCAGGTTCTGAAg                                                                                                                                                                                                                         >  1:725265/1‑239 (MQ=255)
             ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCCGAAGGCGAAACCGCCGGTCAGGGATTGGGCGGCCCGGTCCTGAAg                                                                                                                                                                                                                         >  1:1063364/1‑239 (MQ=255)
                     attatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCGGTATGCGGCCTGCTGAAGGCGAAACCGGCGGTCAGGGCTTGGGAGGCCAGGTTTTAAAGGGGATTaa                                                                                                                                                                                                                 >  1:424277/1‑239 (MQ=255)
                          gggCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGCTGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGGTAAAACGGCGGTCACGGGGTGGGGGGCCCGGGTGCGAAGGGGATTAACaaa                                                                                                                                                                                                             >  2:698011/1‑238 (MQ=255)
                          gggCGTGCGCGGCTTTAACGGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAGCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAAt                                                                                                                                                                                                            <  1:803036/239‑1 (MQ=255)
                               gTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGTCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGCGTTGGGCGGCCAGGTTCTGAAGGGGATTAACGAATcca                                                                                                                                                                                                         >  1:1276677/1‑238 (MQ=255)
                                 gcgcGGGTTTAACTGGGGGCTGGATTTCACCGGTGGGACGGTTAATGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATccacc                                                                                                                                                                                                       <  1:383878/238‑1 (MQ=255)
                                 gcgcGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCAGGGGTTGGGCAGCCCGGTTCTGAAGGTGATTAACGAAAccccca                                                                                                                                                                                                      >  1:732504/1‑239 (MQ=255)
                                     gttttttagtGGGGGGTGTTTTTCACGGGTGTGACGGTTGTTGAATTTAGGCGCGAAAACCGGGCGTAAAGTGACGTAATGCGTGATGCATTGCAAAAGGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCGTGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCa                                                                                                                                                                                                  <  2:1114258/230‑1 (MQ=255)
                                     ggttttAACGGGGGGCTGCATTTCACCTGTGGTACGGTTATTGAAATTCCGCCCGAAAAACCGGCTGAAATTGCCGAAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCa                                                                                                                                                                                                  <  1:1111756/236‑1 (MQ=255)
                                         ttAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGaa                                                                                                                                                                                               <  1:1240085/238‑1 (MQ=255)
                                          tAACTGGGGGCTGGATTTCCCCGGTGGGTCGGTTATTGAAATTACGCTCAAAAACCCGGCTGAAATTGACGTAATGGGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                             <  2:743859/239‑1 (MQ=255)
                                          tAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                             <  1:174945/239‑1 (MQ=255)
                                                 gggCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCCAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGGGGTCCAGCTTTGGGCAGCCAGGTTCTGAAGGAGCTTAACGGACCCACCCATCAGAATGCACCAGt                                                                                                                                                                                      >  1:1238209/1‑239 (MQ=255)
                                                 gggCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGGTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCCGt                                                                                                                                                                                      >  1:966311/1‑239 (MQ=255)
                                                 gggCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCAGGGGTTGGGCAGCCAGGTTCTGAAGGGGGTTAACGAATCCACCAATCCGAATCgagcagg                                                                                                                                                                                      >  1:1076236/1‑238 (MQ=255)
                                                  ggCTTGATTTCACAGGTGGTGCGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGCCGTAAGGCGTTATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                      <  2:155323/238‑1 (MQ=255)
                                                    gtGGATTTCCCGTGTGGTTGTGTTTTTAAAATTTCGTCTGAAAAACCGGGTGAAATTGCGCGAATGGGTGATTCATGGCAAAAAGCCGGTTTTGAAGAGCCGATGCCGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                                    <  2:677038/237‑1 (MQ=255)
                                                     gggggttCCCCGGGGGTACGGTTAGTGAAAGTTAGCTAGAAAAACCCGCTGAAATGGAGGTAATGGCTTATGCATTGCAAAAAGCGGGTGTGTAAGAGCCGATGCGGAAAAACTTTGGTAGCAGCCATGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGaa                                                                                                                                                                                   <  2:206298/233‑1 (MQ=255)
                                                     gggATTTCCCCGGTGGTATGGTTATTTAAATTACGCTGGAAAAACCGGCTGAAAGTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGaa                                                                                                                                                                                   <  2:871245/237‑1 (MQ=255)
                                                                        gttttttGAAAGTACGCCCGAAAAACCGGCTGAAATTGAGGTAATGCGTGATGCTTGGAAAAAGGCCGGTTTTGAAGGGCCTGTGCTGCAAAACTTGGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTc                                                                                                                                                              <  2:1076236/234‑1 (MQ=255)
                                                                          tttttGAAATTACGCTCGAAAAACCGGCTGAAAGTTAAGTTATGAGGTATGCATTGCAAAAAGCCGGTTTGTAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCAGGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTTTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTTCg                                                                                                                                                            <  2:1294711/236‑1 (MQ=255)
                                                                           tttggAAATGACGTGCAAAAAACCGGTGGAAATGTCGGTAATGGGAGGTGCATGGAAAAAGGCGGGTTTTGAAGAGTCGAGGCTGCAAAACTTTGGTGGGAGCAATGTCATGATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCg                                                                                                                                                            <  2:531140/234‑1 (MQ=255)
                                                                           tATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAAAATGCAGCAGGGAAGCGGATTGAGTTCGTTGGTCCGa                                                                                                                                                           >  1:619673/1‑239 (MQ=255)
                                                                              tGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGGCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                        <  1:1238729/239‑1 (MQ=255)
                                                                              tGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                        <  1:816333/239‑1 (MQ=255)
                                                                               taaaTTACGCCAGAAAAACCGGCTAAAATTGACGTAATGCGGGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGt                                                                                                                                                       <  1:827564/238‑1 (MQ=255)
                                                                                  aTTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTTGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCCGGGAAGAGTATTGAGGTCGGCGGTCCGAGCGgggg                                                                                                                                                    >  2:287798/1‑239 (MQ=255)
                                                                                    tACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGCCGGACAGGTGTTGGGCAGCCCGGTTCTGAAGGGGATTAACACAACCACCAATCAGAATGCAGCAGTGAAGCGAATGTGGTTCGTCGGTCGGAGgggggggg                                                                                                                                                  >  2:28163/1‑239 (MQ=255)
                                                                                     aCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGc                                                                                                                                                 <  1:446039/239‑1 (MQ=255)
                                                                                     aCGCTCGAAAAACCGGCTGAAATTGACGTAAGGCGTGACGCATTGCAAAAGGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGc                                                                                                                                                 <  1:936570/239‑1 (MQ=255)
                                                                                      gatctgAAAAACCTGGGTGAAGTTGACGTTATGGGTGGTGGATTGCAAAACAGGAGTTTGGGGGGGGCGAGGCTGCAAAACGTGGGTAGGAGCGATGGCATCATGGTCCGTATGCCGCATGCTGAAGGCGAAACCGGCGGTCAGGTTTTGGGCAGCCAGGTTATGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGTGTATTGAGTTCGTCGGTCCGAGCGTGGGGGc                                                                                                                                                 <  2:131511/234‑1 (MQ=255)
                                                                                        cTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGGTTGGCATCCAGGTGCTGAAGGGGGTTAACGAACCCACCAATCAGAATGCACAAGGGAAGAGGACTGGGTTCGCTGGTCCCAGCGggggggagg                                                                                                                                               >  2:1185127/1‑235 (MQ=255)
                                                                                         tCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAc                                                                                                                                             <  1:113311/239‑1 (MQ=255)
                                                                                         acGAAAAACCGGCTGAAATTGACTTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATTCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGACAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAc                                                                                                                                             <  1:657640/238‑1 (MQ=255)
                                                                                                aCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGTGTGGGCAGCCCGGTTCTGAAGGGGGTTAACGAATCCACCAATCAGAATGCAGCAGGGAAGGGGATGGGGTTCGTCGGTCCCGACCGggggggggtcctttaaa                                                                                                                                       >  2:227779/1‑228 (MQ=255)
                                                                                                      cTGAAATTGACGTAAGGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAc                                                                                                                                  <  1:261114/238‑1 (MQ=255)
                                                                                                               acttAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                        <  1:420141/236‑1 (MQ=255)
                                                                                                                         tGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGaa                                                                                                                                                                                   >  2:21591/1‑171 (MQ=255)
                                                                                                                         tGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGaa                                                                                                                                                                                   <  1:21591/171‑1 (MQ=255)
                                                                                                                         tGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGTATTGGGTTCGGCGGTCCGAGCGGGGGGGCAGACCTTGCGCAAACCGGGGCGATGGCGTtggtgg                                                                                                              >  1:73921/1‑239 (MQ=255)
                                                                                                                            tGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGGATCCACCAATCAGAATGCAGCAGGGACGCGTATTGAGTTCGTCGGTCCGCGCGTGGGGGCGGCCCTGGGGCCAACCGGGGGGGTGGGGTTGATggggg                                                                                                           >  2:944434/1‑236 (MQ=255)
                                                                                                                              gtttcaaaaatGCGGGTTTTGGAGGTGCGATGGCGTCAAACTTTGGTGGGAGCCCTGACAGCATGGTGCGTATGCCGCCTGGTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcg                                                                                                         <  2:740717/233‑1 (MQ=255)
                                                                                                                               aTTGCAAAAGGCCGGTTTGGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcgc                                                                                                        <  1:1322072/239‑1 (MQ=255)
                                                                                                                                 tGCAAAAGGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTGGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  1:482283/239‑1 (MQ=255)
                                                                                                                                 tGCAAAAAGCCGGTTTTGAAGGGCCGATGGTGCAAAACTTTGGTAGCAGCCATGGCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  1:1185127/239‑1 (MQ=255)
                                                                                                                                 tGCAAAAAGCCGGTTTTGAAGAGCCGGTGCTGCAAAACTGTGGTAGCCGCCATGACAGTAAGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  2:219780/239‑1 (MQ=255)
                                                                                                                                 tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  1:698011/239‑1 (MQ=255)
                                                                                                                                 tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGGGCGGGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGGTGATGgcggcggg                                                                                                       >  1:1248289/1‑236 (MQ=255)
                                                                                                                                  tgaaaaaGGCGGTTTTGTAAGGGCCGATGCTGCAAAACTTGGGTGGCAGCCATGACAGCAGGGTCCGTATGCCGCCGTCGGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  2:591906/237‑1 (MQ=255)
                                                                                                                                    aaaagGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctgct                                                                                                    <  1:13968/238‑1 (MQ=255)
                                                                                                                                    aaaaaCCGGGTTTTGAGGTGCCCATGCTGAAAAACTTGGGTAGCAGGCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAACCCGGCGGTCAGGTGTTGGGCAGCCAGGTTCCGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGCCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctgct                                                                                                    <  2:1022828/238‑1 (MQ=255)
                                                                                                                                     aaaaGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGGAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGt                                                                                                  <  1:919448/239‑1 (MQ=255)
                                                                                                                                      aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATTGCGTTGATGGCAggcggggtt                                                                                                   >  1:345383/1‑229 (MQ=255)
                                                                                                                                        aGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGGGCGCTGGCGTTGATGGCAGCGCTGCTGTCTa                                                                                               >  1:681564/1‑239 (MQ=255)
                                                                                                                                        aGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGAGGCGCTGCTGTCt                                                                                                >  1:117526/1‑238 (MQ=255)
                                                                                                                                          ccGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGGGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGGGCTGCTGTCTAt                                                                                              >  1:200499/1‑238 (MQ=255)
                                                                                                                                              ttttGAGGAGCGGGTGGTGCAAAACTTTGGTAGCAGCCATTACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                          <  2:117526/238‑1 (MQ=255)
                                                                                                                                                                       cTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                    <  2:609041/141‑1 (MQ=255)
                                                                                                                                                                                   cgctgtgatcttGGTCCGTTTGCCGCTGGGTGAAGGCGAAACCGGCGGTCAGGGGTTGGGGAGCCCGGTTATGAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggcgg                                                     <  2:200499/232‑1 (MQ=255)
                                                                                                                                                                                     tgttcatcctGGTCCGTATGCCGCCTGGTGAAGGGGAAACCGTCGGTCAGGTTGTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                   <  2:1106067/235‑1 (MQ=255)
                                                                                                                                                                                     aTGACATCTGGGTTCGTAGTGCGGCTGCTGAAGGCGAAACCGGGGGGCAGGTGTTGGGCATCCAGGGTCTGAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                   <  2:579599/239‑1 (MQ=255)
                                                                                                                                                                                        gaataatGGTCGTTATGGCGCCTGGGGAAGGCGAAACCGGCGGTAAGGGGTGGGGCAGACAAGTTCATGAGGTGATTAACGAATCCACCAATGAGAAGGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGTCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                   <  2:748961/231‑1 (MQ=255)
                                                                                                                                                                                         cggcttGGGCCGTATGCCGCCTGGTGAAGGCGAAACCGGCGGTGAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAACGGAATGCAGCAGTGGAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAgggg                                               <  2:1276677/234‑1 (MQ=255)
                                                                                                                                                                                         catcagGGTCCGTATGCCGCCTGCTTAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAggg                                                <  1:105934/238‑1 (MQ=255)
                                                                                                                                                                                                  ccGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACAGATCCACCAATCAGAATGCAGCAGGGAAGGGGATTGAGTTCGTCGGTCCGAACGTGGGGGCAGACCTTGCGCAAAACGGGGCGATGGGGTTGGTGGCAGCGCTGCTGTCTATCCTCGGGGAGGTGGGTTTCCGCTTGGAGAGGGGACTGGGGGCCGgggggggttatg                                      >  2:498683/1‑233 (MQ=255)
                                                                                                                                                                                                   cGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGGGTACGTAGGTTTCCGCTTTGAGGGGCGCATGGCGGCCGGGGGGGTTATTgc                                     >  1:404656/1‑239 (MQ=255)
                                                                                                                                                                                                       tGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCt                                  <  1:1081824/238‑1 (MQ=255)
                                                                                                                                                                                                            ccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTTGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCa                            <  1:287798/239‑1 (MQ=255)
                                                                                                                                                                                                            ccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATAAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTCCGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTTGGCTgggcc                             >  1:180324/1‑235 (MQ=255)
                                                                                                                                                                                                              tgcggAAGGGGAAACCGGGGGTCAGGGTTTGGGGAGCCGGGTTCTGGAGGTGATTAACGAATCCCACAATCGGAATGCAGCAGTGAAGCGTATTGGGGTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGGCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCac                           <  2:619673/238‑1 (MQ=255)
                                                                                                                                                                                                               gctgAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGGTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGGTGATGGGAGAGCTGCTGTCTATCCTCGTGTGCGAAGGTTTCCCCTTTGAGTGGCGACTGCCGGCGGGGGGGGGTATTGTGCGGGGGCccga                         >  2:905033/1‑239 (MQ=255)
                                                                                                                                                                                                                  tgaGGCGAAACCGGCGGTCAGGTTTCGGGCAGCCAGGTTCGTAAGGTGTTTAACGAATCCGCCAAGCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGGCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  2:332974/237‑1 (MQ=255)
                                                                                                                                                                                                                  gtaGGCGAAACCGTGGGTCGGGTTTTGGGCAGGCTGGTTCTGAAGGGGATTAACGAAACCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCGGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  2:467532/237‑1 (MQ=255)
                                                                                                                                                                                                                  gagtgggAAACCGGGGGTTAGGTGTTGGGCAGGCGGGTTTTGAAGGTGATTAACTAATCCACCAATCAGAATGCAGCAGTGGAGCGTATTGAGTTTGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  2:158637/233‑1 (MQ=255)
                                                                                                                                                                                                                  gAAGGGCAAACCGGCTGTTAGGTGTTGGGCCGGCAGGTTCTGAAGGTGATTAACGAATGCGCCGATACGGATGCAGCAGTGAAGCGTATTGAGTGCGGCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  2:367067/239‑1 (MQ=255)
                                                                                                                                                                                                                  gAAGGCGAAACCGGTGGTGGGGTGTGGGGATGCCAGGTTCTGAAGGTTAATAACGGATCCACCAATCCGAAGGCAGCAGTGAAGCGTGTTGAGTGCGTCGGTCCGAGCGTGGGGGAAGGCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTTCGTAGGTTTCCGCTTTGAGTCGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  2:920530/239‑1 (MQ=255)
                                                                                                                                                                                                                    aGGCCAAACCGGCTGGTAGGTTTGTGGCAGGCCGGTTTTGAAGGTTATTAACGAAGCCTCCAAGCAGGATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGtt                     <  2:73921/238‑1 (MQ=255)
                                                                                                                                                                                                                            ccGGGGGTCGGGTGTTTGGCGGCCAGGTTATTAAGTTGATTAACGAATCCACCAACCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTTGTTATTGCGCTGGCGCACGACGTTATCATTACg            <  2:578585/239‑1 (MQ=255)
                                                                                                                                                                                                                             cgggggGCAGGGTTTGGGCAGCCAGGTTCTGGAGGTGAGTAACGAATCCCCCAGTCAGGATGCAGCAGTGAAGCGTATTGAGTTCGGCGGTCCGAGCGTGGGGGCAGGCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGc           <  2:1248289/239‑1 (MQ=255)
                                                                                                                                                                                                                               gcggTCAGGTGTTGGGCAACCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGGGGTTATTTCGCTGGGGCCCGCGGTTAtactttggctg         >  1:1320895/1‑229 (MQ=255)
                                                                                                                                                                                                                                 ggTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATGAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTgg        <  1:655559/238‑1 (MQ=255)
                                                                                                                                                                                                                                       ttgtTGGGCAGGCAGGTTCTGCAGGTGAGTAACGAATCCGCCATATAGAAGGCAGGCGTGGTGGGTATGGAGTTCGTCGGTCCTAGCGTGGGGGCAGACCTTGCGAACACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTTCGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTAttt  <  2:266733/237‑1 (MQ=255)
                                                                                                                                                                                                                                        tgtTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTAttt  <  1:944434/237‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             
CTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTT  >  NC_000913/429601‑430066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: