| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 766,738 | A→G | 28.0% | E252G (GAG→GGG) | mngA → | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 766,738 | 0 | A | G | 28.0% | 43.7 / 2.2 | 27 | E252G (GAG→GGG) | mngA | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component |
| Reads supporting (aligned to +/- strand): ref base A (12/7); new base G (4/4); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTAC‑GTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATC‑AACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCG > NC_000913/766557‑766974 | tGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTAC‑GTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAACAAAACCAAAATTGACACACCCGGTGTTTTTTGGCGTGGAGTGGCGATCAGGGGAGGAGAGGGTTTTAATGGCTTTCCCCCACTTCCCGTCCCCGTTGCGGACCCGAtag > 2:610587/1‑237 (MQ=255) atatGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTAC‑GTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATC‑AACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGGGGGGGAGCGGTTTAATGGCATTTCCGCGCTTTCAGGGCCTTTTGCCGAGCCCATTCGCCATGCCGAAGCGTTTTTCcaacaa > 1:455948/1‑239 (MQ=255) cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTAC‑GTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATC‑AACTCAATAGTGCAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGGGAGTGAGCGGTTTAATGGCATTCCCCCGCTTTCCAGGCCTGTTTCCGCGCCCGTTCGCCATGCAGAACAGCTTATCCCACAAACGCt > 1:1193120/1‑238 (MQ=255) gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTAC‑GTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATC‑AACTCAATAGTGCAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGGTTTAATGGCATTCCCGCGCTTTCAATGACTGTTGCCGAGCCCATTCCCCCATCCGAAAGGGTGATCCCACAAGGCCTTa > 1:1013391/1‑238 (MQ=255) gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTAC‑GTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATC‑AACTCAATAATGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATTGCATTCCCGCGCTTTCCGTGCCTGTTGCCGGACCGGTTCCCCATGCCGAAGCGTTTGTCcaacaaaacgttt > 1:12593/1‑231 (MQ=255) aggcgcgccaaaaCTGGGGATAAATGTTTACGGTTAAAAAAAAGGGCGATAGCGGCGTGGAGGGGCTTTGAACGGCGAATA‑AACAAAATGGTACAGCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGAt < 1:498748/229‑1 (MQ=255) tctacccAATGGTACAGCCGCCTGTATGTTTGCGGATGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGGGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGa < 1:1310840/232‑1 (MQ=255) aaTAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCCCTGTTGAGCGGAATCTCttt > 1:196424/1‑239 (MQ=255) aaTAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGCTGATCCAACAATCGCTTACCCTCAAGCGTAGCGATgag > 1:1228012/1‑156 (MQ=255) tGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGGGAGTGAGCGTTTTAATGACATTCCCGCGCTTTCAGCGCCGAGTGCCGAGCCGATTCGCCATGCAGAAGCGGTGACCCAACAAGCGCTTACCCTCAATCGCAGCGATGAGACGCGGACCTTACCACAAGATACCCAACCGGTGAAAAGAGTCAAAACGGGGCTAAAAAAGCAACGGTTGAGCGAACACCCTTTTGcgg > 2:464682/1‑237 (MQ=255) tGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGcc > 1:761601/1‑238 (MQ=255) cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCa < 1:104021/166‑1 (MQ=255) cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCa > 2:104021/1‑166 (MQ=255) cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGt > 1:1103676/1‑238 (MQ=255) cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGGGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGt > 1:389986/1‑238 (MQ=255) cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGGGAAAAGTGTCAAAACGGGGCTGCAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGt > 1:607464/1‑238 (MQ=255) aaCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGtc > 1:167976/1‑237 (MQ=255) ccGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCg > 1:326238/1‑239 (MQ=255) ggggtggAAGTCCCAATCGAGGGGAGGGGGCGTTTTAATGGCATTTCCGCGGTTTCAGTGCCTGTTGCCGGGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCggg < 1:1281785/233‑1 (MQ=255) gCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAAACGCGCACCGTACAGCCAGGTACGCAACCGGTGAAAATTGTAAAACCGGCGGCTAAACAGGCACGTGTAAGGGGAATCCCTTTTGCCGTACCGGTGAATAGAGCgggggg > 2:530698/1‑239 (MQ=255) gcatgcgcaagcAAGGAGAGTGACCGTTTTAATGGCATTCCCGCGCTTTCATGGACTGTGGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCa < 1:899198/227‑1 (MQ=255) gAAGTCGACATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCa < 1:404052/238‑1 (MQ=255) aaGGGGAGTGGGCGTTTTCAGGGCATTCCCGCGCTTACAGGGCCTGGTGCCGAGCCGATTCGCCATGCAGAAGCGTTTATCCAACAAGCGCTTACCCTAGAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGAGTGTCGCGGGGGGCACGGTGCTGGCGGt < 2:326238/239‑1 (MQ=255) aaGGGGAGTGAGGGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGGGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCTCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGt < 1:615439/239‑1 (MQ=255) aaGGGGAGTGAGCGTTTTAATGGCATGCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGt < 1:1241482/239‑1 (MQ=255) aaGGAGAGTGAGCGTTTTAATGGCATTCCCGGGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTAAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGt < 1:428247/239‑1 (MQ=255) aGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTA‑CCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTcg < 1:178231/239‑1 (MQ=255) | TGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTAC‑GTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATC‑AACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCG > NC_000913/766557‑766974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |