Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 825,248 G→C 21.3% G287G (GGC→GGG ybhR ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913825,2480GC21.3% 96.4 / 3.5 54G287G (GGC→GGGybhRputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base C (10/2);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 4.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

ACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAGCGGCGAAACC  >  NC_000913/825014‑825482
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
aCGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGGGAAACGTAACCGGAAAGGAGAATGGCGGGCACCTTCAAACCAAAAACGccca                                                                                                                                                                                                                                           >  1:442325/1‑237 (MQ=255)
       aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACGATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCCTAAAGAAAAACAACGCGATaaaag                                                                                                                                                                                                                                     >  1:1170016/1‑236 (MQ=255)
       aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGGATCATAAAGAAAAAAACGCCGATAAACg                                                                                                                                                                                                                                     >  1:932817/1‑238 (MQ=255)
       aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAAAACGcccgaaaaag                                                                                                                                                                                                                                     >  1:616357/1‑230 (MQ=255)
       aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGGTAATCCAAGTTCGGTTTTGCAGCCCTACCGGCCGGGTTTCCACCGGAGAAACGTAACCGGAAAGGGGAAAGGCGGGCATCCTAAAGAAAAAAACGCCCAGaaaaa                                                                                                                                                                                                                                     >  2:919682/1‑236 (MQ=255)
        aCATCGGGTACCCCTCTGCCCCTGTCGTGGCCGTTACCACCAGTAGCGGCCACAACCTATCCCAAACAATACCCAGACTCGCATCCTTCAAATAAATCTGTTTGGTAATGTCCGTAAAGGGGCAAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAAAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGAAAAACACGCCGATAAACGcc                                                                                                                                                                                                                                   <  1:586059/239‑1 (MQ=255)
            cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCGTCATAAAGAAAAAAAACCCGATAAAAGCct                                                                                                                                                                                                                                  >  1:260705/1‑236 (MQ=255)
            cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCCTAAAGACCAAAACACCCGTAAACGCccgct                                                                                                                                                                                                                               >  1:170512/1‑239 (MQ=255)
            cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACCCCCATCAACGCct                                                                                                                                                                                                                                  >  1:148078/1‑236 (MQ=255)
            cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGAATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGGATCATAAAGAGCAAAACCCCGATAAAAGccc                                                                                                                                                                                                                                  >  1:937052/1‑235 (MQ=255)
             gcgTACGCCGCTGACCCTTCCTTGGCCTTAACCCCAAGTAGCGGCCAAAAACTATCCCACAAAATATCCAGCCTCGCTTCCTTCAAATAAACCTGTTGGGTAATGTCCGTAAAGTGGCAAATAGGGTTAACCCACTTAAGGTTTGGCAGCCATACCGGCATGTTTTCCACCGGAAAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCctgctg                                                                                                                                                                                                                              <  1:620549/239‑1 (MQ=255)
              cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACCCCGGTAAACGCCTGCcgt                                                                                                                                                                                                                             >  1:1166454/1‑239 (MQ=255)
               gTACCCCGCTGCCCCTGTGGTGGCCGTTATCACGAGTAGGGGCAAAAAATTTTTCCCCACAATACCAAGACTCCCATCCTTAAAAAAAATCTGTTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAACCCACGTAAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAAAAACGTAACCGGAAGGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCtgtt                                                                                                                                                                                                                            <  1:389140/239‑1 (MQ=255)
                           cccTTTGGTGCCCGTTTCCCCCAGTAGCGCCCACAAACTTTCCCCAACAATACCAGGCCCCGAATCTTTAAAAAAATTCTGCTTGGTAAGGCACGAAAAGGGGCAAATGGGGTTAACCCAGGTCAGGTTTTGCAGCCATCCCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACaga                                                                                                                                                                                                               <  2:1166454/239‑1 (MQ=255)
                                    ggCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAAACAAACACGCCGATAAACGCCCGCTGGTGGGGTGAACaagagggttgaa                                                                                                                                                                                                      >  1:1247951/1‑228 (MQ=255)
                                     gCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCTTAAAGACAAACACGCCGCTAAAAGCCCGCCGTTGTGTTTAACAGAGGGATGAAAt                                                                                                                                                                                                    >  1:762030/1‑239 (MQ=255)
                                      ccGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGAGGCGAATAGGGTTAATCCACTTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGTGGTCATCATAAAGACAAACACCTCGATTACAGCCTCGCGTTGTGTTTGAAAagagagtaaaaac                                                                                                                                                                                                     >  1:694743/1‑230 (MQ=255)
                                        tttATCCCCATTGCCGCCCACAATTTGTTCCACACAATACCAAGACTGCATCCCTAAAAAAAATCCTGCTGGGAATGTTCCTAAAATGGGGGAATAGGGTAAACCCCCGTCAGTTTTGGAAGCCATACCGGCATTTTTCCCACGGGAAAAACTTAACGGAAAAGGAGATTGGGGGGAAACAAAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCaa                                                                                                                                                                                                 <  2:585749/238‑1 (MQ=255)
                                                        gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGGAGCCTAAAAACAAAAACCCCGCAAAAACCCCGCTGTTGTGTTGAACAACATGATGAAAACAAAAAAACGAAAaccacc                                                                                                                                                                                 >  2:1014227/1‑239 (MQ=255)
                                                        gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAAAACGCCGATAAACGCCCGCTGTTGTGTTTAACAGCGGGGTGAAAACCACAGACCGAATCccac                                                                                                                                                                                  >  1:459423/1‑238 (MQ=255)
                                                          cacaAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATCGGGTTAATCCACGTCAGGTTTTGCAGCCAAACCGGCATGGTTTCCACCGGAGACAACTAACCGGAAAGGAGAATAGCGGGGATCGCGAAAACAAACAACCCGAAAAAAACCACCTGTTGTGTGGCAAAGAGAGATGAAAACACAAGACCGAAACCCCCCAg                                                                                                                                                                               >  2:833023/1‑239 (MQ=255)
                                                                         acaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGGATCCTAAAGACAAACACACCGATAAACGCCTGCTGTTGGTTTGAAAAAATGGATGAAATCAAAAAACCGAATCCCCCCAGCGATAAACCATaaa                                                                                                                                                                 >  1:114765/1‑238 (MQ=255)
                                                                             tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACCCCGATAAACGCCGGCTGTTGTGTTGAACAGAGTGATGAAATCAAAAGACCGAATCCCACCACCGGTAAACCATAAATCAcc                                                                                                                                                            >  1:749689/1‑239 (MQ=255)
                                                                             tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATAACGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGAATCTTAAAGACAAAACAGCCCAGAAACGCCACCTTGTGGGTTGTACAGAGGGTTGAAAACAAACAACCGAACCCCACCACCCGTAAAcacaaaaaaaca                                                                                                                                                            >  2:984217/1‑228 (MQ=255)
                                                                                 cAGACTCGCATCCTTCAAATAAATCTGTTTGGTAATGTCCGTAAAGGGGCAAATGGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATAATAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCg                                                                                                                                                        <  1:856289/239‑1 (MQ=255)
                                                                                         cATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGGACAACAACCCGATAAACGCCCGCTGTTTGGTTGAACAGAGGGAATAAATAAACAAACCCAAACCACCCCACGATAAAAAAAAAAACACCAACCTaaaaaaa                                                                                                                                                >  2:1188282/1‑235 (MQ=255)
                                                                                         cATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAAAACGAATCCCACCACCGATAAACCATAAATCACCATCTTaaaagag                                                                                                                                                >  1:812194/1‑235 (MQ=255)
                                                                                         cATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCCGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCCCCACCGAGAAACCAAAAATCACCCCCGTAAAgaaa                                                                                                                                                >  1:367521/1‑236 (MQ=255)
                                                                                                             cTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGGTAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAAACCGAAACCCACCAGCGCTAAACCCTAAATAACCATCGTAAAGTAGAAAAGAGCCAGCGactcg                                                                                                                              >  1:520897/1‑234 (MQ=255)
                                                                                                             cTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTTAACAGAGTGATGAAATCAACAGACCCAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCgg                                                                                                                             >  1:126388/1‑239 (MQ=255)
                                                                                                                     tGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATTATAAAGACCAACAAGCCGGTAAAACCACGCTGTTGGGGTTGACAAAGGGAGTGAAATAACAAAACGAATTCCACCACCAGTAAACCAAAAAACACACCCGTCAACAAGAAAAACGCCGCCCAACCGGGGAAgggg                                                                                                                     >  2:754882/1‑239 (MQ=255)
                                                                                                                              atggggCGAAAGGGTTAAACCCCATTCGGTTTTTGCAGCCATACCGGCATTTTTTCCACCGGAAAAACGTAACGGGAAAGGAGAATGGCGGGCATAAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATa                                                                                                             <  1:984217/235‑1 (MQ=255)
                                                                                                                                          gggTTATCCCACTTCAGATTTGGAGCCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATAc                                                                                                 <  1:20950/238‑1 (MQ=255)
                                                                                                                                            gTTAACCCGCGTCAGGTTTTGCAGCCATCCCGGAATGTTTTCCACCGGAAAAACGTAACCGGAAAGGAGAATGGCGGGAATCAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCa                                                                                               <  1:1014227/238‑1 (MQ=255)
                                                                                                                                                ctccgccgaaGGTTTTGCAGCCATCCCGGCATGTTTCCCACGGGAAAATCGTAACCGGAAAGGAGAATGGCGGTCATCAAAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGc                                                                                          <  1:1230393/230‑1 (MQ=255)
                                                                                                                                                acccccGTCAGGTTTGCCACCCATCCCGGCTTTTTTCCCCCGGGAAAACCGTAACCGGAAAGGATAATGGCGGGCATAAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGc                                                                                          <  1:1074978/234‑1 (MQ=255)
                                                                                                                                                   ccggTCAGGTTTTGCAGCCATCCCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAg                                                                                       <  1:112457/236‑1 (MQ=255)
                                                                                                                                                     ggttaGGTTTGGCGGCCATCCCGGCATGTTTTCCACCGAAAAAACGTAACCGGAAAGGAGAATGGCGGGCATAAAAAAGCAAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTAGATACGCCCAGATACCAATCGCCAGca                                                                                     <  1:405762/235‑1 (MQ=255)
                                                                                                                                                     cGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGGTAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAGGGGGATTTGAAAGCCCCCGAAACCACTccccccca                                                                                     >  1:898513/1‑235 (MQ=255)
                                                                                                                                                     cGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAAACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAAAAGCGCCAACGAACCGGGGAAGGGGAATTGAAACCGCCCAAAACCAATCGCCAGcc                                                                                     >  1:434764/1‑238 (MQ=255)
                                                                                                                                                                                       cACGGGAAAAACGAACCCGAAAAGGAGATTGGGGGGAAAAAAAAAGAAAAACACGCCGATAAACGCCTGCTGTTGGGTTGAACAGAGTGATGAAAGCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACg                                                    <  2:434764/239‑1 (MQ=255)
                                                                                                                                                                                       cACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGGGGCCTGGAAGGGGGCGACAATTaaa                                                     >  1:1094314/1‑237 (MQ=255)
                                                                                                                                                                                                      cccGAAAAGGAGAATGGCGGCAAACATAAAGACAACCTCGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGc                                     <  1:964620/238‑1 (MQ=255)
                                                                                                                                                                                                      aCCGAAAAGGAGAATGGCGGGCATAATAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGc                                     <  1:527361/239‑1 (MQ=255)
                                                                                                                                                                                                        cgaagaggggtaTGGGGGGCATAATAAAGAAAAACACGCCGATAAACGCCGTCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCCCCAGCGATAAACCATAAATTCCCATCGTAAAGTAGAACAGCGCCAGCTATCCGGGGAAGGGGATTTGATTCGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCg                                   <  2:204276/228‑1 (MQ=255)
                                                                                                                                                                                                           aagggAGAGTGGCGGTAATAATAAGGCACAACACCCCGTAAAACGACCGCTGTGGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGGGATAAACCATAAATCACCAGCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGAt                                 <  2:613985/235‑1 (MQ=255)
                                                                                                                                                                                                             aGGAGAATGGGGGGCATAAAAAAACAAACCACCCCGATAAACGCCTGTTGCTGTGTTGAACAGGGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGaa                              <  2:749689/239‑1 (MQ=255)
                                                                                                                                                                                                             aGGAGAATGGCGGATATAAAAAAGAAAAACACGCCGATAAACGCCTGGTGTTGTGTTGAACAGAGTGATGAAAGCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCCCAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGaa                              <  1:545955/239‑1 (MQ=255)
                                                                                                                                                                                                                              catAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGTGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGGGGCTGCAATTAACGCCGGTACGGCTTTGCCGATGAAGGTCTGCCAGGGGGGGa             >  2:1030022/1‑239 (MQ=255)
                                                                                                                                                                                                                                  aaagcaaaccacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAgcg          <  1:754882/230‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCACGCCGATAAACGCCTGCTGTTGGGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACCCCAAGATCCCAACCCCCAGCACCATGGGCGCCTGGAAGGGCGCGCGATTTACACCCGGAACGGGCTTCCCGTGGAATATCGGCAAGGGGGGGAGCGGGGaaa  >  2:1033805/1‑238 (MQ=255)
                                                                                                                                                                                                                                         accacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAGCGGCGAAAc   <  1:1279645/237‑1 (MQ=255)
                                                                                                                                                                                                                                         accacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTACCGATGAAGATCTGCCAGGTGGTGAGCGGCGAAAc   <  1:749693/237‑1 (MQ=255)
                                                                                                                                                                                                                                         acacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCCCAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAGCGGGGAAAcc  >  1:844732/1‑239 (MQ=255)
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ACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAGCGGCGAAACC  >  NC_000913/825014‑825482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: