BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,357,427	T→C	10.1%	E81G (GAG→GGG)	ymjA ←	DUF2543 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,357,427	0	T	C	10.1%	268.8 / 3.2	100	E81G (GAG→GGG)	ymjA	DUF2543 family protein
Reads supporting (aligned to +/- strand): ref base T (53/36); new base C (5/6); total (58/42)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 105 total aligned reads displayed.

CGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATA  >  NC_000913/1357190‑1357657

cGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAAAAACTTAGAGAGGAGCAGAGCGGTACGATTGTGAGAACCCCATTAAAGCACGTGGCAATGCGAGGCAGTGTTATCCAGGTGAGGCGTGAACGCTTTCTCCGGCAAACGATAACGACCGATCGGCTGCAAATAAAtca‑ttattc                                                                                                                                                                                                                                         >  2:795882/1‑239 (MQ=255)

  tAATGGGGGCCGTGTTGTTGGTCGGTGAACAGGTGACGTCGCCATCTGTTCATCATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCTTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtca‑ttactcat                                                                                                                                                                                                                                       <  1:549401/239‑1 (MQ=255)

     tGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtca‑ttactcattac                                                                                                                                                                                                                                    <  1:169025/239‑1 (MQ=255)

     tGAGGGCCGTGTGGGTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtca‑ttactcattac                                                                                                                                                                                                                                    <  1:142801/239‑1 (MQ=255)

         ggCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGCCAACGATACTGTCCGAACGTCTGCCATTAAATAA‑TTATCCATTAcccaa                                                                                                                                                                                                                                >  1:51263/1‑237 (MQ=255)

            cGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTAAACGCTTTATCCGGACAAAGCTACTGACCCACCGCCTGCAATTAAATCC‑TTACCCTTAAACCCATTg                                                                                                                                                                                                                             >  2:244406/1‑239 (MQ=255)

              tgtgGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATCCGCCGTGAACGCTTTATCCGGACAACGAAACTGACCGACCGTTTGCAATTAAATCA‑TTCCCCATTACCCCCTTTAt                                                                                                                                                                                                                           >  1:1010373/1‑239 (MQ=255)

                           tGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGc                                                                                                                                                                                                              <  1:741027/239‑1 (MQ=255)

                                 ggTGTCGTCGCCATCTGTTCAACATTCGTACCCGTGATTCCTCTATACCTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGCCATTGTTAGCCAGATGCGGCGTGCACGCTTTATCCGGACACAGCTACTGACCGATCGCATCCAATTAAACCA‑TTACTCATTACCCCCTTGATTCAAAAACTATGC‑G‑Acct                                                                                                                                                                                                       >  1:764603/1‑238 (MQ=255)

                                  gTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGCTACTGCCCGATCGGCTGCAATTAAAGCA‑TTCCTCATTACCCCATTGATTCACAAACCCTGC‑G‑ATCcca                                                                                                                                                                                                     >  1:514851/1‑239 (MQ=255)

                                       gTCGCCATTTGTTCAACATTCGTACCCGTTATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAAt                                                                                                                                                                                                <  1:795882/239‑1 (MQ=255)

                                            cATCTTTTCAAATTTCGGACCCGTGATTTCCCTATAACTATAACTCACCAAACAACTTGGCGTGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGGTCGTGCCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTa                                                                                                                                                                                           <  2:796650/239‑1 (MQ=255)

                                                         ttCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGCCTGCAATTAAATCC‑TTACTCATTACCCCCTTAATTCAAAAACTCTGC‑A‑ATTCCATCAATACATAAGGGATAAAAcc                                                                                                                                                                               >  2:496876/1‑238 (MQ=255)

                                                               cccGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACCGGATTAATCCCCCCTTc                                                                                                                                                                        >  1:554737/1‑239 (MQ=255)

                                                                 cGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGCTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAAGTAAATAA‑TTGCTCATTACCCCATTGAATTAAACACTATGC‑G‑GTCTCATAAATGCGTAAGCGACTAATCCCCACTCcac                                                                                                                                                                      >  2:1250472/1‑238 (MQ=255)

                                                                  gTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCCCATCAATGCTTACGGGATTAATCCCCCCTTcacc                                                                                                                                                                     >  1:1305577/1‑237 (MQ=255)

                                                                     aTTTCTCTAGAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGGGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTcagcag                                                                                                                                                                   <  1:1170530/238‑1 (MQ=255)

                                                                        tctcTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGCCTGCAATTAAATCA‑TTACTCATTACCCACTTGATTCAAAAACCTTCG‑G‑ACCCCACCAATGCTAAAGGGGTTAAACCCCCCtcacacaccaca                                                                                                                                                               >  2:126833/1‑228 (MQ=255)

                                                                          tctATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGAAACTGACCGATCGTCTGCCATTAAACCA‑TTACCCATTACCCCCTTGATTACAAAACCCTCC‑A‑GTCCCATCAATCCTAAAGGGCATAAACCCCGCATCACCAGCCAttt                                                                                                                                                             >  2:695643/1‑239 (MQ=255)

                                                                                    taactCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACACCGATACTTACCGACCGACTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTTC‑G‑ATCTCATCAATGCGACAGGGCTTAATCCCCGCTTCAGCAGCACTTTAAAGTTgg                                                                                                                                                     >  2:177760/1‑237 (MQ=255)

                                                                                    taactCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCaaaa                                                                                                                                                                                                                     <  1:195035/175‑1 (MQ=255)

                                                                                    taactCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCaaaa                                                                                                                                                                                                                     >  2:195035/1‑175 (MQ=255)

                                                                                    taactCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCCAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTgg                                                                                                                                                     >  1:778862/1‑237 (MQ=255)

                                                                                    taactCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGAACGGGATTAATCCCCCCTTCCGCAGCCATTTCATGTTgg                                                                                                                                                     >  1:354137/1‑237 (MQ=255)

                                                                                      actCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTTATTCAAAAACCCTGC‑G‑GTCCCATCAATTCGTACGGGATTAATCCCCCCTTCACTAACCATTTCATTTTGGGctt                                                                                                                                                 >  2:751026/1‑239 (MQ=255)

                                                                                      actCAAAGAACAACTTAGCGAGGAGCAGAGCGGTAAGATCGTGAGAAACCCATTAAAGCACGGTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGctt                                                                                                                                                 <  1:695643/239‑1 (MQ=255)

                                                                                      accccaaGAACAACATAGCTGGGGCTAGGGCGGTTCGAACGTGAAAAACCCTATAAAGCACGCTGCAGTTAGTGTCATTGTTAGCCAGATGCGGCGTTAACGCTTTATCCGGACAACGATACTGACCGATCGTATGCAATTAAATCA‑TTACTCATTTCCCCATTGATGCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCGTCAGCAGCCATTTCATGTTGGGctt                                                                                                                                                 <  2:423287/233‑1 (MQ=255)

                                                                                          acaGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGcttttt                                                                                                                                              >  1:788131/1‑238 (MQ=255)

                                                                                           caGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATTTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTCCACATTGGGcttctt                                                                                                                                              >  1:1083379/1‑237 (MQ=255)

                                                                                            aGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCAt                                                                                                                                                          >  1:770765/1‑224 (MQ=255)

                                                                                            aGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGAAGCCATTTCATGTCGGGCTTCTTCga                                                                                                                                           >  1:423287/1‑238 (MQ=255)

                                                                                            aGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAAATCTGC‑G‑ATCTCATCAATCAGAACGGGATTAATCCCCGCTTCAGCAGCCAATTACTGTTGGGCtccttc                                                                                                                                             >  1:1154260/1‑237 (MQ=255)

                                                                                                  acTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCaaa                                                                                                                                                                                                                      >  1:101831/1‑160 (MQ=255)

                                                                                                  acTTAGCGAGGACCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCaaa                                                                                                                                                                                                                      <  2:101831/160‑1 (MQ=255)

                                                                                                   cTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTCCTCATAACCCCATTGATTCAAAAACTCTGC‑G‑ATCACATCAATGCGCACGGGAGTATTAACCGCTTCAGCACCCATTTCATATTGGGCTTTTTCCCTGAttc                                                                                                                                     >  2:607790/1‑236 (MQ=255)

                                                                                                   cTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAAACCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAACCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTTACCGATCGTCTGCAATTAAATCA‑TTACCCATTACCCCCTTGATTCAAAAACTCTGG‑A‑GTACTATAAATGCGAACGGGACAAATCACCGGTTAAGCAGCCATTTCATGATGGGTATCTTCGCtaagc                                                                                                                                      >  2:516753/1‑233 (MQ=255)

                                                                                                        cGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTCC‑G‑ATCTCATCAATGCGTACTGGATTAATCCCCCCTTCACCCGCCATTTCCGTTTGGGCTTCTTCGCTAATttctcatt                                                                                                                               >  2:728822/1‑235 (MQ=255)

                                                                                                         gggtagCAGAGCGGTACGATTGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATTCTGACCGATCGTCTGCAATTAAATCA‑TTACCCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCAt                                                                                                                               <  1:752272/234‑1 (MQ=255)

                                                                                                             cgCAGAGCGGTACGATCGCGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGAGGCGTGAACGCTTTATCCGGACAACGATACTGACCGCTCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGCTTCAAAAACCTTGC‑G‑ATTCCATCAATCCGACAGGGATTAATCCCCCATTACCAACCCCTTCCAGGTTGGGCTTCTTCGCTGATCTCTTCATTGtt                                                                                                                           >  2:537317/2‑238 (MQ=255)

                                                                                                             agCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCCCTTCATTGtt                                                                                                                           >  1:966628/1‑238 (MQ=255)

                                                                                                                 tgtgcgTACGAGCGTGAGCATCCCTTTAAAGCAGTATTTCGTGCCTTGTATTGGTAGCATGATTCGGCGTGAACGCGTTATCCGGACAACCATACTGACCGAACGTCTGCAATTAAATCA‑TTCCTCATTACCCCAATGATTCAAAATCTCTGC‑G‑ATCTCTTCAAGTCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTtcatca                                                                                                                      <  2:661304/234‑1 (MQ=255)

                                                                                                                 gagGGGTACGATCGTGAGAACCCCATTAAAGCACGCTGCGGTGCGTGTCATTGTTAGCCAGAGGCGGCGTTAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTtcatca                                                                                                                      <  2:970800/239‑1 (MQ=255)

                                                                                                                 gagCGGTACGATCGTGAGAAACCCATTAAAGCCCGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTtcatca                                                                                                                      <  1:1290731/239‑1 (MQ=255)

                                                                                                                       tcgtttggTGGGAAACCCATTAAAGCACGCTGCAGTGCTGGTCCTTGTTAGCCTGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGTTCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTTC‑G‑ATCTCATCAATGCTTACGGGATTAATCCCCGCTTCAGCAGCCATGTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACgg                                                                                                                 <  2:722468/231‑1 (MQ=255)

                                                                                                                       tACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACgg                                                                                                                 <  1:244406/238‑1 (MQ=255)

                                                                                                                       tACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCCCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTTATCTCTTCATTGTTCATCAAAAggg                                                                                                                >  1:1220508/1‑239 (MQ=255)

                                                                                                                        acgagtgTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACggg                                                                                                                <  1:516753/232‑1 (MQ=255)

                                                                                                                         cGATCGTGAGAAACCCATTAAAGCCCGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTg                                                                                                              <  1:751026/239‑1 (MQ=255)

                                                                                                                           gtccttaaaaaCCCTTTTAAGCACGTTGCAGTGAGTGTTATTGTTTGCCAGTTGCGGCGTGAACGCTTTATCCGGACAACGATCCTGAACGATCGTCTGCAAGTAAATCA‑TTTCTCATTACCCCATGGATTCAAAAACTCTGC‑G‑ATCTCATCAGTGGGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTACTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAg                                                                                                            <  2:107914/231‑1 (MQ=255)

                                                                                                                            tCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGc                                                                                                           >  1:837752/1‑239 (MQ=255)

                                                                                                                             ttggagaAACCCATTAAAGACCGCTGCAGGGCGTGTCATTTTTACCCAGAGGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTCCTCATTCCCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGTGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCa                                                                                                          <  2:788131/236‑1 (MQ=255)

                                                                                                                              gTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCCTTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGGGAGCAg                                                                                                         >  1:533836/1‑239 (MQ=255)

                                                                                                                                 agaAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATTTTGGGCTTCTTCGCTGATCTCTTCATTGTTAATCAAACGGGTGAGCAGt                                                                                                        >  1:1222096/1‑237 (MQ=255)

                                                                                                                                 agaAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGGGAGCAgga                                                                                                       >  1:1285655/1‑236 (MQ=255)

                                                                                                                                      cccATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGctt                                                                                                                                                 <  2:149873/191‑1 (MQ=255)

                                                                                                                                      cccATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGctt                                                                                                                                                 >  1:149873/1‑191 (MQ=255)

                                                                                                                                      cccATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATAGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTAATCAATGCGTACGGAATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCCTTGTTCATCAAACGGGTGAGCAGTAACTGGa                                                                                                 >  1:1318242/1‑239 (MQ=255)

                                                                                                                                                 aCGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGAACGGGATTAATCCCCGCCTCACCAGCCATTTCATTTTTGGCTTCTTCGCTGATCTCTTCATTTTTCCTCAAACGGGTGAGCCGAAACTGGAAGTAGTGGGc                                                                                       >  2:500854/1‑238 (MQ=255)

                                                                                                                                                   gCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAg                                                                                    >  1:593337/1‑239 (MQ=255)

                                                                                                                                                   gCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAACAGCCATTTACTGTTGGGCTTCTTCCCTTATCTCTTCTTTTTTCTCCAAACGGTTGGGCAGTAACt                                                                                                    >  2:1297202/1‑223 (MQ=255)

                                                                                                                                                       cAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTTACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAACCGGGTGAGCAGTAACTGGCAGTAGTGGGCCCGCGgt                                                                                >  1:1054699/1‑239 (MQ=255)

                                                                                                                                                        cttgtgtgtttttGTTAGCCAGATTCGGCTGTAACGGTTTTTCCGGACAACGAATTTGACCGATCGTCTGCAATTAAATCA‑TTCCTCATTACCCCATTGATTCAAAAACCCGGC‑G‑ATCTCATCAATGCGTTCGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGgtg                                                                               <  2:258244/228‑1 (MQ=255)

                                                                                                                                                         gTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGgtg                                                                               <  1:342543/238‑1 (MQ=255)

                                                                                                                                                                 cATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGCGG‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGGGCGTTc                                                                        >  1:900203/1‑238 (MQ=255)

                                                                                                                                                                    tGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGgtg                                                                               <  1:356659/227‑1 (MQ=255)

                                                                                                                                                                    tGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGcc                                                                    >  1:1117632/1‑238 (MQ=255)

                                                                                                                                                                    tGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCct                                                                   <  1:496876/239‑1 (MQ=255)

                                                                                                                                                                     gTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACCCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCctc                                                                  <  1:126833/239‑1 (MQ=255)

                                                                                                                                                                          cccagtGCGGCGGTAACTCTTTATCCGGCACACGTTTCTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGa                                                             <  2:354137/234‑1 (MQ=255)

                                                                                                                                                                              aTGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑a                                                                                                                                                                                                          <  1:670677/94‑1 (MQ=255)

                                                                                                                                                                              aTGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑a                                                                                                                                                                                                          >  2:670677/1‑94 (MQ=255)

                                                                                                                                                                              aTGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCCTTGTTCATCAAACGGGGTAGCAGTAACTGGAAGTAGTGGGCCACGGGTGGGCTTTCAGc                                                                     >  2:356659/1‑227 (MQ=255)

                                                                                                                                                                               tggggcgTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAgg                                                         <  1:500854/235‑1 (MQ=255)

                                                                                                                                                                                         aCGCTTTATCCGGACAACGATTCTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCAtt                                              <  1:350014/239‑1 (MQ=255)

                                                                                                                                                                                         aCGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTTTTCATCAAACGGGTGCGCAGTAACTGGAATGAGTGGGCCAGCGGTGTTCGTACAGCCTCTGCGAAAggaccgggggaat                                              >  2:637338/1‑228 (MQ=255)

                                                                                                                                                                                           ccTTTATCCGGACCACGATACTGACCTATCGTCTGCAATTAAATCA‑TTACTCATTGCCCCATCGATTCAAAATCTCTGC‑G‑ATCTCATCCATGCGTACGGGAGTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCg                                            <  2:1305577/238‑1 (MQ=255)

                                                                                                                                                                                                 gccGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTcgcg                                      <  1:50866/238‑1 (MQ=255)

                                                                                                                                                                                                   cGGACAACGATACTGGCCGATCGTTTGCAATTAAATCA‑TTCCCCATTACCCCAATGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTa                                    <  1:537317/239‑1 (MQ=255)

                                                                                                                                                                                                          cGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTg                                                                                                                                                      <  1:434226/118‑1 (MQ=255)

                                                                                                                                                                                                          cGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATACGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTg                                                                                                                                                      >  2:434226/1‑118 (MQ=255)

                                                                                                                                                                                                             tACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCa                          <  1:990028/239‑1 (MQ=255)

                                                                                                                                                                                                               cTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAat                        <  1:532364/239‑1 (MQ=255)

                                                                                                                                                                                                                  aCCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGTCCAGCGGTTTGCGTTCAGCCTCTGCGCCAGGCCCTGCCAATTCGGTCGCGAACTCATCGGCAATATc                     >  2:209480/1‑239 (MQ=255)

                                                                                                                                                                                                                  aCCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATc                     >  1:680296/1‑239 (MQ=255)

                                                                                                                                                                                                                  aCCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCCCGGACTCATCGGCAAAATc                     >  2:532364/1‑239 (MQ=255)

                                                                                                                                                                                                                   ccgtatgttcgCAATTAAACCA‑TTACCTCGTCCCCCAATTATTCAAAAACCTTGA‑G‑ATCTTATCAATGCGTACGGGATTAAGCCCCGTTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCa                    <  1:506688/229‑1 (MQ=255)

                                                                                                                                                                                                                   cccattttCTTCAATTAAATCATTTCCTCA‑TACCCCATTGACTCAAAACCCCTGC‑C‑ATCCCAATCATGCTTTCGGGATTAATCCCCGTTTCAGCATCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCa                    <  2:1309243/232‑1 (MQ=255)

                                                                                                                                                                                                                    cgcatcttttgaaTTAAAACA‑TTTCCCATTACCCCATGTGCTCAAAAACGTCGG‑T‑ATCTTATCAATGCGTTCGGGATTAATCCCCGCTTCAACAGCCATTTCATGTTGGGCTTTCTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCa                    <  2:572807/227‑1 (MQ=255)

                                                                                                                                                                                                                    cGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGGGAGCAGTAACTGGAAGTAGTGAGCCAGAGGTGTGCGTTCAGCCTCTGCGACAGGCTTGGAGCATTCGGTGCGGTACTCCTCGGCAATATCaa                   >  2:275864/1‑239 (MQ=255)

                                                                                                                                                                                                                       tCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGTTGTGCGTTCAGCCTCTGCTACAGGCCATGCGCATCCGGTCGCGTACACATAGGAAAAATCaaaca                >  2:60098/1‑237 (MQ=255)

                                                                                                                                                                                                                       tCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTTGGCGTTCGGCCTCTGCGACAGGCTCTGCGCATTCGGTCCGGTACTCATCGGCAAAATCaaaaa                >  2:924152/1‑238 (MQ=255)

                                                                                                                                                                                                                       tCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGACGCGTACTCATAGGCAACATCAAAat                >  1:853246/1‑239 (MQ=255)

                                                                                                                                                                                                                          tCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGAAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGGGCATTCGGTCGCGTACTCATCGGGCATATCaaacaatt             >  1:734140/1‑234 (MQ=255)

                                                                                                                                                                                                                           tcgCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATAttt            <  1:523892/237‑1 (MQ=255)

                                                                                                                                                                                                                            tGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTTTGGGACCGGCTCGGCGCATTCGGTCGGGACCTTATCGGCAAATTCAAAATAtttt           >  2:323697/1‑239 (MQ=255)

                                                                                                                                                                                                                            tGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGACCTCATCGGCAATACCAAAAAAtttt           >  2:401014/1‑239 (MQ=255)

                                                                                                                                                                                                                              catttAAATTC‑TTGCTCATTCCCCCATTTA‑TCCAAAACCCTGG‑GCATTTCTTTAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCGCTTCAGTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAg         <  2:966628/236‑1 (MQ=255)

                                                                                                                                                                                                                              cAATTAAATCA‑TTCTCCTTTCCCCCATTGTTTCAAAAACTTTGC‑G‑ATTTTATCAATGGGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAg         <  2:593337/239‑1 (MQ=255)

                                                                                                                                                                                                                                     aTCA‑TTCCTCCTTTCCCCATTGATTCAAAAACTCTGC‑G‑ATCCCATCAAGGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGata  <  2:1117632/239‑1 (MQ=255)

                                                                                                                                                                                                                                           tactcattacCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCAt                                               >  1:1025988/1‑189 (MQ=255)

CGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCA‑TTACTCATTACCCCATTGATTCAAAAACTCTGC‑G‑ATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATA  >  NC_000913/1357190‑1357657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`