Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	154,321	A→C	11.1%	V369G (GTC→GGC)	htrE ←	putative outer membrane usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	154,321	0	A	C	11.1%	258.7 / 3.2	90	V369G (GTC→GGC)	htrE	putative outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base A (69/7);  new base C (2/12);  total (71/19)
Fisher's exact test for biased strand distribution p-value = 1.41e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCA  >  NC_000913/154086‑154557                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               ggAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATTTCATCTTTTAAGAc                                                                                                                                                                                                                                               >  1:393514/1‑237 (MQ=255)      cacGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTACCAAACCAGCGGAATAGTTCTTA‑TCGGTCATCTGACTACCGG‑TATAA‑CCCGTCCGATAGTTATTCAGGCCGACGTAGTAGCTTGTATAAAATAACTTAGAGCAC‑CCCTGAATACCATATTTTCAGCACTGAcc                                                                                                                                                                                                                                         >  2:423869/1‑238 (MQ=255)      cacGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGAcc                                                                                                                                                                                                                                         >  1:1177469/1‑238 (MQ=255)           tAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCt                                                                                                                                                                                                                                   >  1:384438/1‑239 (MQ=255)                 tGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAATAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCCTCTTTTAAAACCTGACCGCCGCaaaaaa                                                                                                                                                                                                                              >  2:485417/1‑235 (MQ=255)                        ggTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAAAATCATCTTTTAAGACCTGACCGCCGCTAAAATCCCaaact                                                                                                                                                                                                                      >  2:769259/1‑236 (MQ=255)                              ttttATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑CATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGAAGTAGCTGGCTTGAACTAAATTAG‑GACTATCCTGAATACAATCTTTAAAGACCTGACACCCACTAAAAACCCAACGTCAAAc                                                                                                                                                                                                                 >  2:571284/1‑238 (MQ=255)                                   tcatcCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAAAGTCCAAACCCAgg                                                                                                                                                                                                           >  2:56593/1‑239 (MQ=255)                                        cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCTAACGCCAGGGCGT‑a                                                                                                                                                                                                     >  1:1145730/1‑239 (MQ=255)                                        cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑a                                                                                                                                                                                                     >  1:694389/1‑239 (MQ=255)                                        cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑a                                                                                                                                                                                                     >  1:343515/1‑239 (MQ=255)                                        cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTAGAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCATGCTTACAATAAATTAG‑GCACATATTAAATACCATCTTTTAAACCCGCACCGCCCATAATCACCCAACGTCCAACCCCaagtggca                                                                                                                                                                                                      >  2:810195/1‑232 (MQ=255)                                                     ttGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGaacaac                                                                                                                                                                                        >  1:140811/1‑239 (MQ=255)                                                          aTGAGTCAAACCGAAAAAAAATGCCCCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCGGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGAt                                                                                                                                                                                    <  1:612699/238‑1 (MQ=255)                                                                       aaagaaaATGCACCAACTGAAGAATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGGAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTACCAGCAGCTAATATCCAAACGACCAAC‑CCACAGCATAAACATTCGAAAAACGAATGAAAAAGATTgaga                                                                                                                                                                      >  2:384591/1‑239 (MQ=255)                                                                        aaaaaaaTGCCCAATCTGAATTATTCAGACAAAGACCTAACAACCCAGGGGTATATTTATTA‑TCGTTTATCGGAATCCGGG‑TATAC‑CCCTTCAGATAGTTATTCAGCCCTGTGTAGTAGCTTGCTTAAAATAAATTAG‑GCTCACCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCgag                                                                                                                                                                     <  1:1150194/236‑1 (MQ=255)                                                                             aaTGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAACTAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGG‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATg                                                                                                                                                                 >  1:983658/1‑238 (MQ=255)                                                                             aaTGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATg                                                                                                                                                                 >  1:355428/1‑238 (MQ=255)                                                                                       cTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAAACCTGACCGCCGCTAATATCCCAACGTCCAACCCCAGGGCTT‑ACCATTTGAACACAGGTTGAGAAAGGTTGCGAGAATGTCCGCTTTGa                                                                                                                                                       >  2:759202/1‑238 (MQ=255)                                                                                               ttCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCTG‑AAAATTTGAACAACGGTTAGGAAAGGTGGCGAGAAAGTCCCCTTTGGGCCCCCGGa                                                                                                                                              >  2:906568/1‑239 (MQ=255)                                                                                                    aCCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGAttct                                                                                                                                          >  1:148882/1‑238 (MQ=255)                                                                                                    aCCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCAGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGCCCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACACCGGATGCGAAAGGGTGAGAGAAATACACCTTTTGGCCAACGGAttctt                                                                                                                                         >  2:107752/1‑239 (MQ=255)                                                                                                         gACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGCTg                                                                                                                                    >  1:118573/1‑239 (MQ=255)                                                                                                           ccaaacaacCCGGGGGTATAG‑TATTAGTCGGTTA‑CTGAAATCCGGTTAAAC‑CCCGTAAGATAGTTATTCAGGCCGTAGTATTAGCTTGCTTAAAAAAAATTAG‑GCTCATCCTGAATACCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                  <  1:769259/236‑1 (MQ=255)                                                                                                           ccaaacaacCCAGCGGTATAGTTATTA‑TCGGTTATTTGAATACCGG‑‑ATAACCCCGTAAAATATTTATTAAGGCCGTAGTAGTAGTTTGCTTAAAAAAAATTAG‑GCTCATCCTAAATACCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                  <  1:56593/236‑1 (MQ=255)                                                                                                           ccaaaaaaaCCAGGGGTATTGTTATTT‑TGGGTTATCTAAATACCGT‑TAAAC‑CCCTTCAGATTGTTATTCAGGCCGTAGTAGTAGCTTGCTTAAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                  <  1:541451/233‑1 (MQ=255)                                                                                                           ccaaaCAAACCAGGGGTATAGTTATTA‑TCGTTTAGCTGAAACCCGG‑TATAA‑CCCGTAAATTAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAAAAAATTAG‑CCTCATCCTGAATATAATCTTTAAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                  <  1:1240843/236‑1 (MQ=255)                                                                                                           ccTAACAAACCAGGGGTATAGTTATTA‑CCGGTTATCTGAATACCGT‑TATAA‑CCCGTCAGATAGTTATTCAGGCCTTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                  <  1:265501/239‑1 (MQ=255)                                                                                                           ccTAACAAACCAGCGTTTTGTTTATTA‑GCGGTTATCTGAATACCGG‑TATAC‑CACGTAAGTTAGTTATTCAGGCCGTAGTAGTAGCTTGTTTGAAAAAAATTAG‑GCTCATCCTGAATACCATCTTTAAAGCCCTTACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                  <  2:218280/239‑1 (MQ=255)                                                                                                              aaCAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCga                                                                                                                                                                      >  1:543907/1‑200 (MQ=255)                                                                                                              aaCAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGGGAATGTCCGCTTTGAGCCATCGGATTCTTCGTTGGTAAc                                                                                                                               >  1:218280/1‑239 (MQ=255)                                                                                                              aaCAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAc                                                                                                                               >  1:161662/1‑239 (MQ=255)                                                                                                                        cGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCAGCTTTGAGCCATCGAATTCTTCGATGGTAACAAAAAGAtg                                                                                                                      >  1:311751/1‑237 (MQ=255)                                                                                                                                  atta‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAAAAAGATCGCTTCCGTAc                                                                                                            >  1:742365/1‑238 (MQ=255)                                                                                                                                     a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑CACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTg                                                                                                        >  1:73559/1‑239 (MQ=255)                                                                                                                                     a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGGACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGAATCTTTGATGGTAACAATAAGAACGCTGCCGTAACCTg                                                                                                        >  2:232512/1‑239 (MQ=255)                                                                                                                                     a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTAcctga                                                                                                        >  1:1098803/1‑236 (MQ=255)                                                                                                                                     a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCt                                                                                                         >  1:353970/1‑238 (MQ=255)                                                                                                                                     a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAAAAAGATCGCTGCCGTACCCta                                                                                                        >  1:569003/1‑238 (MQ=255)                                                                                                                                     a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCCATGGTAAAAAAAAAATGGCGTCCGAACCCta                                                                                                        >  2:1104657/1‑238 (MQ=255)                                                                                                                                              tCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCAATGGGAACAATAAAATCGCGGCCGTACCCTTACGGACTc                                                                                                >  2:982598/1‑239 (MQ=255)                                                                                                                                                         g‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCTCTGCCGTACCCTGACGGACTCAGACCATAAAt                                                                                     >  2:1126649/1‑239 (MQ=255)                                                                                                                                                             taA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGACCAACGGATGAGAAAGGTTGCGCGAATGGCCGCTTTGAGCCATCGGATTCTTCGATGGTAACACTAGAAACGCTGCCGTACCCTGCCGAACCCAAatctgtttctta                                                                                  >  2:624613/1‑230 (MQ=255)                                                                                                                                                             taA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTACAATCATCAATGAc                                                                                  >  2:526612/1‑239 (MQ=255)                                                                                                                                                               a‑CCCTTCAGATAGTTATTCAGGCCATAGTATTAGCTTCATTGAAATAAATTAG‑CCACCTCCAAAATATCATTTTTTAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACAGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGa                                                                                <  1:1315617/239‑1 (MQ=255)                                                                                                                                                                   cGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGaaa                                                                              >  1:373174/1‑238 (MQ=255)                                                                                                                                                                    gTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCAATCGGATTCTTCGATGGTACAAATAAGATCGCTGCCGTACCCTGACGAACTCAAATCATCAATAACGaacg                                                                             >  1:135231/1‑236 (MQ=255)                                                                                                                                                                      cAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAccg                                                                           >  1:453811/1‑238 (MQ=255)                                                                                                                                                                         aTAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGCGAATGTCCGCTTTTAGCCATCGGATTCTTCGATGGTAACAATAAAATCGCTGCCGTACCCAGACGGACCCAGATCATCAATGAACAAAggcccgg                                                                       >  2:283202/1‑233 (MQ=255)                                                                                                                                                                             ttATTCAGCTCTTAGTAGTTGCTTTGTTTAAATAAATTAG‑GCTAATCCTTAATACCATTTTTTAAAACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTggcgg                                                                   <  1:851717/239‑1 (MQ=255)                                                                                                                                                                                ttCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGCTGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGGACCCTGCCGGACCCAGATCATCAATGAAGAAAGCGCCTGGCGGCa                                                                 >  2:837343/1‑238 (MQ=255)                                                                                                                                                                                     gCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGTCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCACGAATATCCGCTTTGTGCCATCGGATTCTCAGATGGTAAAACTAAGATCGCTCCCGCACCCTGAGGGACTCGTGTAATCAATAACAAACACGCCTgccgccccccccg                                                           >  2:425193/1‑230 (MQ=255)                                                                                                                                                                                      ccGTAGTATGTGCTTGCTTGAAAAAAATTAG‑GCTCAACCTAAATATCATTTTTAAAGCCCGGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑Ccgtcg                                                          <  1:526612/238‑1 (MQ=255)                                                                                                                                                                                             tgttggttgtttaAAATAATTTTG‑GCTCCTCCTGAATAACATTTTTAAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATaa                                                  <  2:643733/233‑1 (MQ=255)                                                                                                                                                                                               tagCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATaa                                                  >  1:415362/1‑237 (MQ=255)                                                                                                                                                                                               tagCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATAGCTGCCGTACCCTGACGGACTCAGATCATCAATGCAGAAAGCGCCTGGCGGCA‑CCGTGGTGTCAaaaaa                                                >  2:726860/1‑238 (MQ=255)                                                                                                                                                                                                 gcttgcttGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTTACGGACTCAAATAATCAATGACGAAAACGCCTGCGGGCA‑CCCTCGTTTCATAAATCt                                              >  2:1271135/1‑239 (MQ=255)                                                                                                                                                                                                 gcttgcttGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCCGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCt                                              >  1:1152393/1‑239 (MQ=255)                                                                                                                                                                                                    tgcttgCAATCAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGGAACAATAAGATCGCTGCCGTACCCACACGTACACAGATCCTAAATGACAAAAGGGCCGGCCGGCA‑CCGCCGTTTCATAAATCAtaa                                           >  2:747678/1‑238 (MQ=255)                                                                                                                                                                                                    tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AGCATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTtat                                           >  1:98957/1‑239 (MQ=255)                                                                                                                                                                                                    tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAAt                                                                                                                            >  1:974187/1‑159 (MQ=255)                                                                                                                                                                                                    tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTtat                                           >  1:1321856/1‑239 (MQ=255)                                                                                                                                                                                                    tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGAC‑CCGTCGTTTCATAAATCTtat                                           >  1:468218/1‑239 (MQ=255)                                                                                                                                                                                                    tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAAATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACCAAAACGCCCGGCGGCA‑CCACCCTTTTATAAAACTtaa                                           >  2:966630/1‑238 (MQ=255)                                                                                                                                                                                                    tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTCCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGCTGGGAACAATACAACCGCTGCAGTACCCTGACGGACCTGGATCATAAATAACGAAAACCCCTCACGGAC‑CCGCCCTTTCATaacatcaaa                                           >  2:1094109/1‑232 (MQ=255)                                                                                                                                                                                                      cttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGTCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGTTAACAATAAGATCCCTGCCGTACCCTGACGGACTCAGATCATCAATAACGAAAACACC‑GGCGGGACCCGCCGTTTCAAAAACCtataa                                          >  2:639137/1‑234 (MQ=255)                                                                                                                                                                                                      cttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTtata                                          >  1:531274/1‑238 (MQ=255)                                                                                                                                                                                                      cttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTtata                                          >  1:832816/1‑238 (MQ=255)                                                                                                                                                                                                      cttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAAAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAACGCCGTGCGGCA‑CCGTCGATTCATaaaattcaaag                                         >  2:1001351/1‑231 (MQ=255)                                                                                                                                                                                                           aataattTGG‑GCCCCTCCTAAATACCTTTTTTAAAGCCCGTACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  1:652273/239‑1 (MQ=255)                                                                                                                                                                                                           aataaatTCG‑CCCCCCCCTAAATTTCATCTTTAAACCCCTGACCGCCGCTAAATCCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  2:373174/239‑1 (MQ=255)                                                                                                                                                                                                           aaaaaatTAG‑GCTACGCCTGAATACCTTTTTTAAAGCCCGTGCCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  1:742449/236‑1 (MQ=255)                                                                                                                                                                                                           aaaaaatGAG‑GCACATCCTAAAATTCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  1:979/236‑1 (MQ=255)                                                                                                                                                                                                            ataaatTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTTGATGGTAACAATAAGATCGATGCCGTACCCTGACGGACTCAGGTAATCAATGACGAAAGCGCCTGGCGGAA‑CCGCCGTTTCAAAAACCTAAAAGCAAcca                                   >  2:648777/1‑238 (MQ=255)                                                                                                                                                                                                                      gCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGAACGCTGCCGTACCCTGACGGACTAAGATCCTCAATGACGAAATCGCCTGGCGGCA‑CCGTCTGTTCCTAAACATTATAAACCACTTGCGTAAtc                          >  2:742280/1‑238 (MQ=255)                                                                                                                                                                                                                          aTCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAgg                        >  1:203833/1‑236 (MQ=255)                                                                                                                                                                                                                                aaTATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGCAACAATAAGATCGCTGCCGTACCCTGCCGGACTCAGATCATCAATGACGAAAGCGCCTGTCGGCA‑CCGTCGTTTCATAAACCTTATAGCCACCTTGCGTAATAGGGACATTgg                >  2:280299/1‑239 (MQ=255)                                                                                                                                                                                                                                  tctcattTTTAAACACCTGCCCGCCGATAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCg              <  1:772872/237‑1 (MQ=255)                                                                                                                                                                                                                                  tatCTTGTTTAAAGCCCGGCCCGCCGCTAATAACCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCg              <  1:1091778/239‑1 (MQ=255)                                                                                                                                                                                                                                   acactcttgaaaGCCCGGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGt             <  1:1104657/229‑1 (MQ=255)                                                                                                                                                                                                                                    tcatcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTCATAGAGACTTTGGCGtt            >  1:984765/1‑239 (MQ=255)                                                                                                                                                                                                                                     catcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGTACTCAGATCATCAATGACGCAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGttt           >  1:698252/1‑238 (MQ=255)                                                                                                                                                                                                                                     catcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTg           >  1:201119/1‑239 (MQ=255)                                                                                                                                                                                                                                     catcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGGACCCTGACGGACTCAGATCATCATTGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGAAATAGTGAATTTGGGGTTg           >  2:954963/1‑239 (MQ=255)                                                                                                                                                                                                                                      atcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGCCGTTTCATAAATCTTATAGCCACCTTCCGTAATAGTGACTTTGGCGTTgg          >  1:529967/1‑239 (MQ=255)                                                                                                                                                                                                                                       tcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGCCGGACTCAGATCTTAAATGACGAAAGCCCCTGGCGGCA‑CCGTCGTTTCTTAAACCTTATAGCCACCTTGCGTAATAGTGACtt                   >  2:1292615/1‑229 (MQ=255)                                                                                                                                                                                                                                       tcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGt         >  1:603247/1‑239 (MQ=255)                                                                                                                                                                                                                                       tcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACAGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGt         >  1:1168386/1‑239 (MQ=255)                                                                                                                                                                                                                                          tttAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTAtt      >  1:1185763/1‑239 (MQ=255)                                                                                                                                                                                                                                              aGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCa  >  1:1248965/1‑239 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               GGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCA  >  NC_000913/154086‑154557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑