BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,062,718	A→C	13.6%	T623T (ACA→ACC)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,062,718	0	A	C	13.6%	246.2 / 2.1	99	T623T (ACA→ACC)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand): ref base A (55/25); new base C (3/16); total (58/41)
Fisher's exact test for biased strand distribution p-value = 4.10e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGACAGTGTGC  >  NC_000913/3062482‑3062956

ccAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGAACGCGGCGCGAAA‑GAGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCTACGTAGATTTAAA‑CCCGATGTTCTCTaca                                                                                                                                                                                                                                                >  1:1028346/1‑237 (MQ=255)

         tgtgtTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGGTTCGACGTAGGTTTTAG‑CCCGAGGTTCTCTAAACCTGAAaaga                                                                                                                                                                                                                                      >  1:420565/1‑238 (MQ=255)

          gtgtTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGGCACGATCGCGGCGCGAAA‑GTGATCGCCAACGCC‑TATTCCGATCTCGGTTTCGCCGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAagagct                                                                                                                                                                                                                                     >  1:929473/1‑236 (MQ=255)

                ccGGCGTGATTGCGCAAAGCTACCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGAAAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATcgcccgc                                                                                                                                                                                                                              <  1:891929/239‑1 (MQ=255)

                    cGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GGGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTTAAGAGATCGCCCGCCTg                                                                                                                                                                                                                           >  1:369507/1‑238 (MQ=255)

                                 agcttcccttaGTCTGAAAAACTGGCCTCCGAGTTCGATGCCATTGTTTGCCAAACGGGGGAGTTCCTTGCCTACAATGGT‑CGTCG‑CCC‑GCGCAGTCCGATCGCTAAGATGGGCCCGGATGGAAACGATTGCGGCGCGAAA‑GTGATCCCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGACGTAGAAAacga                                                                                                                                                                                                             <  2:1308785/229‑1 (MQ=255)

                                 aGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACCCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAacg                                                                                                                                                                                                              >  1:1308785/1‑238 (MQ=255)

                                 aGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAacg                                                                                                                                                                                                              >  1:462162/1‑238 (MQ=255)

                                 aGCTATACTCAATCTGAGAAAGCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTGGCCGAAAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAacga                                                                                                                                                                                                             <  1:1316235/239‑1 (MQ=255)

                                           aGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTTAAGAGATCGCCCGCCTGACCGTCGAAAACGACGTTCACGTa                                                                                                                                                                                                   >  1:129047/1‑239 (MQ=255)

                                             tCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGt                                                                                                                                                                                                 >  1:14543/1‑239 (MQ=255)

                                             tCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATAGCCCGCCTGGCCGTAGAAAACGACGTTCActaagt                                                                                                                                                                                                 >  1:973524/1‑234 (MQ=255)

                                             tCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GGGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGCAGAGATCGCCCGCCGGGCCGTAGCAAACGCGGTTCACGGAgg                                                                                                                                                                                                 >  1:1180189/1‑238 (MQ=255)

                                             tCTGAGAAATCGGCCTACGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCCCGAAA‑GTGTTCGCCAGCGCC‑TATTCCGATCCCGGTTTCTCCGTACTTTTAGC‑CCCAGTGTCCCCTACCCCTAAAGCAATAGCCCCCCTGGACGGAGAAAAAGACGATCCCggatg                                                                                                                                                                                                 >  2:1140013/1‑235 (MQ=255)

                                              cTGGGAAAGCGGCCCCCGAGTTGGCTCCCCTTGTTGCGCAAACGGACGATTTCCTTGCCGACAAGGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGGTGGGCCAGGAGGGACCCGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTa                                                                                                                                                                                                   <  2:1044682/236‑1 (MQ=255)

                                              cTGAGAAAGCGGGCTCCGAGTTCGATGGCATTGGTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGC‑CGTCG‑CCC‑GCGCATTCTGACCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAg                                                                                                                                                                                                  <  1:876440/237‑1 (MQ=255)

                                                gagaAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGCCGTTCACGTAGTggg                                                                                                                                                                                              >  1:327775/1‑239 (MQ=255)

                                                gagaAATCCGCCTCCGGGGTCGAGTCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACCCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTggg                                                                                                                                                                                              <  1:1030824/239‑1 (MQ=255)

                                                  gaAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAACCGACGTTAACGTAGTGGgc                                                                                                                                                                                             >  1:778153/1‑238 (MQ=255)

                                                           cTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGCTGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTc                                                                                                                                                                                     >  1:243720/1‑237 (MQ=255)

                                                                            ttgttgCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATaaa                                                                                                                                                                  >  1:261755/1‑239 (MQ=255)

                                                                                 tgcccaaCGGGGCGTTTCGTTGCCGACAATGGTCCGACG‑CCC‑G‑GCATTCTGATCGCGAAAGTGGGCCAGGCGGGACGCGATCGCGGCCCGAAAGGTGA‑CGCCAGCGCC‑CATCCCGCTCCCGGTTCCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCt                                                                                                                                                             <  2:420565/234‑1 (MQ=255)

                                                                                 ggccAACCGGGGCGGTT‑CTTTCCGGCAATGGG‑CGGCCCCCC‑GCGCATTGTGATCGCTAAGATGGGCCAGGATGGACCGGAGCGGGCCTCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCGGATGTTCTCTCCACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCt                                                                                                                                                             <  2:1120904/236‑1 (MQ=255)

                                                                                 gcgcAACCGGAGCAGTTCCTTGCCGCAAATGGG‑CGTGG‑CCC‑GCGCATTCTGAGCGCTAAGATGGGCCAGGATGGACCCGTTCGCGGCGCGAAA‑GGGATCGCCAGCCCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACCCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCt                                                                                                                                                             <  2:369507/239‑1 (MQ=255)

                                                                                 gcgcAAACGGAGGAGTTCCTGGCCGACAGTGGT‑CCTCG‑CCC‑GAGCTTTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCt                                                                                                                                                             <  1:563906/239‑1 (MQ=255)

                                                                                 gcgcAAACGGAGCAGGTCCGTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCt                                                                                                                                                             <  1:1130020/239‑1 (MQ=255)

                                                                                   gcAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGCAGAGATCGCCCGCCTGGCCGTAGCAAACGACGTTCACGTAGTGGgc                                                                                                                                                                                             >  1:656998/1‑205 (MQ=255)

                                                                                    cAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCGGa                                                                                                                                                           >  1:1226333/1‑238 (MQ=255)

                                                                                    cAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACCCTGa                                                                                                                                                           >  1:1203951/1‑238 (MQ=255)

                                                                                       aCGGAACAGTTCCTTGCCGACAATCGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GGGATCGCCAGCGCC‑TATTCCGATCCCGGTTTCGACGTGGATTTAAG‑CCCGCTGTCCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAAAGACGATCACCGAGTGGCCGGATCCCCCCGTGCCGCCGGCCATACAACGcccacccc                                                                                                                                                       >  2:362640/1‑232 (MQ=255)

                                                                                                     tGCCGACAATGGT‑CGTCG‑CCC‑GGGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAg                                                                                                                                         <  1:1107372/239‑1 (MQ=255)

                                                                                                     tGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACCCCTGAAGAGATCGCCCGCCTCGCCGTAGTAAAAGCAGTCTCCGAAGAGGGCGCATCCTCACTGGCTGCCGGCCATAAAACGCTGACCCCGGAACTTGTCgga                                                                                                                                          >  2:1266601/1‑236 (MQ=255)

                                                                                                     tGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTg                                                                                                                                                                                                                                            <  1:417351/140‑1 (MQ=255)

                                                                                                     tGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTg                                                                                                                                                                                                                                            >  2:417351/1‑140 (MQ=255)

                                                                                                     tGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGCGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGGGGGCGCATCCTCACTGGCTGCCGGTCAAAAAACGCTGACCCCGGAACGGGTCGaaa                                                                                                                                         >  2:357101/1‑238 (MQ=255)

                                                                                                     tGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCCTAAAACGCTGATCCCGGAACTGGTCGaa                                                                                                                                          >  1:774607/1‑238 (MQ=255)

                                                                                                     tGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGaa                                                                                                                                          >  1:1252408/1‑238 (MQ=255)

                                                                                                     tGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGGCATAAAACGCTGATCCCGGAACTGGTCGaa                                                                                                                                          >  1:976574/1‑238 (MQ=255)

                                                                                                         gaaaagggT‑CGTCG‑CCCTGCG‑ATTTTGATCGCTAAGGTGGGCCAGGATTGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCt                                                                                                                                     <  1:426180/233‑1 (MQ=255)

                                                                                                          aCAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCCGATCCCGGAACTGGTCGAAGCGCTg                                                                                                                                    >  1:1230103/1‑239 (MQ=255)

                                                                                                          aCAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGCCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTg                                                                                                                                    >  1:765067/1‑239 (MQ=255)

                                                                                                                       ccccc‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATggg                                                                                                                          <  1:436807/236‑1 (MQ=255)

                                                                                                                             gcgcATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGGcgcg                                                                                                                    >  1:1004790/1‑239 (MQ=255)

                                                                                                                                  ttCTGATGGCAAAGTGGGGCCCGGCGGGACCCGATCCGGGCGGGAAA‑GTTGTCCCCATGCCC‑TTTTCCGTTCCCGGTTTCGACGTAGATTTAAG‑CCCGATGGTCCCTACCCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCAGCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGat                                                                                                               <  2:323392/239‑1 (MQ=255)

                                                                                                                                       aTCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAC‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCTTAGAAACCGCCGTTTCCGTAGTGGCACCACCCTACCCGGCTGCCTGTCATAAAACGCTGACCCCGGAACCGGCCTCACGCCCGAAAAAAAGGGGAAGGGAAGagaccgg                                                                                                          >  2:643701/1‑233 (MQ=255)

                                                                                                                                           cTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATAACTggttg                                                                                                      >  1:667334/1‑235 (MQ=255)

                                                                                                                                                   ggCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGCTCCCGGGAATGGTCGCAGCGCTGACAAAATGGGGACCCGAGAATATCTGCGGGGGCGCggg                                                                                              >  2:54283/1‑239 (MQ=255)

                                                                                                                                                   ggCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGaa                                                                                                                                                   >  1:447416/1‑186 (MQ=255)

                                                                                                                                                    gCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATG‑TCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCt                                                                                                                                                                                      <  2:124720/149‑1 (MQ=255)

                                                                                                                                                    gCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATG‑TCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCt                                                                                                                                                                                      >  1:124720/1‑149 (MQ=255)

                                                                                                                                                        ggATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGc                                                                                          >  1:861727/1‑238 (MQ=255)

                                                                                                                                                         gATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCGGCCTGGCCGTAGCAAACGCCGTTCACGTAGTGGGCGACTCCCAACTGACTCCGGGGCCTAAAACGCTGACCCCGGAACTGGTGGAACCCCCGAAAAAATGGGGCCCCGAAGAAAT‑TGCGAGGCTGCggggggggg                                                                                        >  2:50276/1‑235 (MQ=255)

                                                                                                                                                              acacGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCCCGTAGCGGGCCCATCCTCACTGGCTGCCGGTCATAAACCGCTGTTCCCGGAACTGGCCGAACCGCGGAAAAAATGGGGACCCGAAAATATCAGAGGGGGCGCggggggggagaatc                                                                                   >  2:678248/1‑231 (MQ=255)

                                                                                                                                                              acacGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCg                                                                                                        >  1:858342/1‑218 (MQ=255)

                                                                                                                                                              acacGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTGAtt                                                                                    >  1:467673/1‑238 (MQ=255)

                                                                                                                                                              acacGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCAtt                                                                                    >  1:782786/1‑238 (MQ=255)

                                                                                                                                                              acacGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGGCAtt                                                                                    >  1:555447/1‑238 (MQ=255)

                                                                                                                                                              acacGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCCCGGGTGGCGTCAtt                                                                                    >  1:437500/1‑238 (MQ=255)

                                                                                                                                                              acacGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGGCATAAAACGCTGATCCCGGAACTGGTCGGACGGCTGAAAAAATGGGCACGGGAAGATACCGTCGTGGACGCGGGGGGCGTCAtt                                                                                    >  2:1267689/1‑238 (MQ=255)

                                                                                                                                                                  gATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGACGAAACCTCCGTGGTCGCGGGGGCGGTCATTccgcc                                                                               >  1:1289908/1‑239 (MQ=255)

                                                                                                                                                                   ttgcgggggggAAA‑GTGA‑CCCCAGCGCCGTTTTCCGTTTTGGTTTTCGACGGAGATTTAAG‑CCCGATGTTCTCTACACCGGAAGATACCGCCCGCCTGGCCGTAGAAAACGACGTTAACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAACCGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTccgccg                                                                              <  2:774607/229‑1 (MQ=255)

                                                                                                                                                                   gtgggggcgCACAA‑GGGATCGCCACGGGC‑TTTTCGGATCCCGGTGTTGACGGAGTCTTAAC‑GCGGATGTTTCCTCACCCTGAAGAGAGCGCCCGCCGGGCCGTAGAAAACGACGTTAACGTAGTGGGCGCATCCTCACGGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTccgccg                                                                              <  2:1028346/234‑1 (MQ=255)

                                                                                                                                                                   gtCGCGGGGCGAAA‑AGGGTCGCCAGCGCC‑TATCCCGAACTCGGTTTCGACGGAGATTTAAG‑CCCAATGTTGCCTACCCCGGAAGAGATCGCGCGCCTGGGCGTAGAAAGCGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTccgccg                                                                              <  2:1289908/238‑1 (MQ=255)

                                                                                                                                                                    gcggggcgCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCGCGGTTTCGACGTAGATTTAAG‑CCCGATGTCCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTccgccg                                                                              <  1:1267689/234‑1 (MQ=255)

                                                                                                                                                                       cggcgCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGCGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGAACGCGAAGATATCAGCGGGGTCGAGGGGGGCGTAATTCTGCCGCAgg                                                                          >  1:985367/1‑239 (MQ=255)

                                                                                                                                                                                a‑GTGATCGCCAGCCCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATTTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCt                                                                 <  1:1140013/239‑1 (MQ=255)

                                                                                                                                                                                          cAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGTGGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAag                                                        >  1:1064731/1‑239 (MQ=255)

                                                                                                                                                                                             cgcC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGCAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGGTTACGCCTTCCTGCAagacc                                                     >  1:158396/1‑237 (MQ=255)

                                                                                                                                                                                             cgcC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAagag                                                      >  1:1191187/1‑238 (MQ=255)

                                                                                                                                                                                                     tCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGCCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGt                                              >  1:818172/1‑239 (MQ=255)

                                                                                                                                                                                                         aTCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTCGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGGCTTGAAAAAATGGGGACCCCAAGATATCTGCGTGGTCGCGGGGGGCGTCATACAGCCCCCGGATTACGCCTTCC‑GCAAGAGCgcggccgggggg                                         >  2:27751/1‑232 (MQ=255)

                                                                                                                                                                                                         aTCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCCCGGGGGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAAAACCCGGCGTggcg                                          >  1:460758/1‑239 (MQ=255)

                                                                                                                                                                                                          tctcGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCCGGATTACGCCTTCCTGCAAGAGCGCggcggggcgg                                         >  1:1017427/1‑239 (MQ=255)

                                                                                                                                                                                                                     gcgttgtttccaG‑CCCGGTTTTCCCCACCCGTGAAGAGCCTCCCCGCCGGGCCGTAGAAAACGCCGTTTCCGTGGTGGGGGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATTTCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGt                              <  2:1230103/228‑1 (MQ=255)

                                                                                                                                                                                                                     gcggaattttAAG‑CCCGATTTTCTCAACCCCGGAGGGGACCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCGCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGt                              <  2:437500/232‑1 (MQ=255)

                                                                                                                                                                                                                     gcGTCGATTTAAG‑CCCGATGTTCCTGACCCCTGCAGCGATCGCCCGCCTGGACTGAGAAAACGGCGTTCACGGAGGGGGCGCATCCTCACTGGCTGCCGGTCCTAAAACGCTGTTCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGCCGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGt                              <  2:1017909/238‑1 (MQ=255)

                                                                                                                                                                                                                      cgtgattgtAAG‑CCCGATGTTCTCTACACCTGAAGAGACCGCCCGCCTGTCCGTAGAAAACGACGTTCACGTAGTGGGCGCAGCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGt                              <  2:462162/230‑1 (MQ=255)

                                                                                                                                                                                                                      cggaaatgtAAG‑CCCGTTTGTCTCTCCCCATGAAGAGAGCGCCCGCCTGGCCGTAGAAACCGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGt                              <  1:935520/230‑1 (MQ=255)

                                                                                                                                                                                                                      cGTAGATTTAAG‑CCCGATGTTCTTTACCCCTGAGGAGATCGCCCGCCTGGCCGTAGAAAACGACGTGCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGGCGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGt                              <  1:689696/238‑1 (MQ=255)

                                                                                                                                                                                                                      cGTAGATTTAAG‑CCCGATGTTCTCTACACTTGAAGAGATCGCCCGACTGGCCGTAGCAAACGACGTTCACGTAGTGGGCGCCTCCTCGCTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACTCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGt                              <  1:176908/238‑1 (MQ=255)

                                                                                                                                                                                                                        gtgATTTTAGCCCCGATCTTCTCTCCACCCGAGGAGATCGCCCGCCTGGCCGGTAAAAACGACGTTCAGGTAGTGGGCGCATCCCCACGGGGTGCCGGTCAAAAAACGCTGAACCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAAATATCTGCGTGGGCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGGAAGAGCGCGGGGTGGCGGCGATTTATGGTcc                            <  2:14543/237‑1 (MQ=255)

                                                                                                                                                                                                                          gttttAAG‑CCCGATGTTCGCTCCCCCTGAAGGGACCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAgg                         <  1:362640/237‑1 (MQ=255)

                                                                                                                                                                                                                          gATTTAAG‑CCCGAGTTTCCCATCACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGAATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAgg                         <  1:638208/239‑1 (MQ=255)

                                                                                                                                                                                                                            tttAAG‑CCCGATTTTCCCTACCCCTGAAGAGATCGCCCGGCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTa                       <  1:1022619/239‑1 (MQ=255)

                                                                                                                                                                                                                            tttAAG‑CCCGATGTTCACTACACCTGAAGAGATCGGCCACCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAgg                         <  1:1126647/237‑1 (MQ=255)

                                                                                                                                                                                                                            tttAAC‑CCCGATGTTCCCAACCCCTGAAGAGACCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTa                       <  1:1068593/239‑1 (MQ=255)

                                                                                                                                                                                                                               aaG‑CCGGATGTTCTGTACACCTGAAGAGGTCGCCCGGCTGGCCGTAGAAAACGACGGTCACGTAGTGGGCGCAACCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATTTCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTacac                    <  1:389695/239‑1 (MQ=255)

                                                                                                                                                                                                                                aacccctgtGTTCTCTACCTCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTGGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAAGGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTacac                    <  1:773559/231‑1 (MQ=255)

                                                                                                                                                                                                                                    cggATTTTCCCTACACCTGAAAAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTAACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCt                  <  1:396998/235‑1 (MQ=255)

                                                                                                                                                                                                                                    ccaaTGTTCCCTACCCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCt                  <  1:1265187/234‑1 (MQ=255)

                                                                                                                                                                                                                                      gATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGGGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTACGGTCCAGGTCAACCTAt                >  1:374903/1‑237 (MQ=255)

                                                                                                                                                                                                                                      gATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGACGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCCGCGTGGTAGCGGGTGGCGTCATTCCGCCGCGGGGTTCAGCCTTCCTGCGAGAGGCCGGCGGGGGGGCGGTTTATGGCTCAGGGAccccatgc               >  2:824453/1‑234 (MQ=255)

                                                                                                                                                                                                                                       aTGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGGGGCGTCATTCGGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGCGGTGGCGGGTGTTTATGGCCCAGGAACACCTATgt              >  1:1326160/1‑237 (MQ=255)

                                                                                                                                                                                                                                        tGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGTGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTTCGTGGTCGCGGGTGGCGGCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGGGATTTTTGGTCCAGGAAAAccaaagccc            >  2:1062213/1‑232 (MQ=255)

                                                                                                                                                                                                                                        tGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATgtt             >  1:827463/1‑236 (MQ=255)

                                                                                                                                                                                                                                          ttCTCTACCCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGa          <  1:54283/239‑1 (MQ=255)

                                                                                                                                                                                                                                            ctctACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGCTTTATGGTCCAGGt                        >  1:115070/1‑223 (MQ=255)

                                                                                                                                                                                                                                            ctctACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGACa        >  1:769823/1‑239 (MQ=255)

                                                                                                                                                                                                                                            ccctccacCTGAAGGGATCCCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGACa        <  1:678248/234‑1 (MQ=255)

                                                                                                                                                                                                                                                  acCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGGGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGCCCGTGTgc  >  1:100811/1‑239 (MQ=255)

CCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGT‑CGTCG‑CCC‑GCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAA‑GTGATCGCCAGCGCC‑TATTCCGATCTCGGTTTCGACGTAGATTTAAG‑CCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGACAGTGTGC  >  NC_000913/3062482‑3062956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`