Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,471	C→G	17.3%	S3R (AGC→AGG)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,471	0	C	G	17.3%	140.0 / 7.3	63	S3R (AGC→AGG)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (10/37);  new base G (6/5);  total (16/47)
Fisher's exact test for biased strand distribution p-value = 5.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206236‑3206709                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    tAAAGGCCAGGTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGa                                                                                                                                                                                                                                                   <  1:1056758/238‑1 (MQ=255) tAAAGGCCAGCTTTTTAAGATTGTTAAATTTGCGCAGTTGGGAAAAGCGCAATGTATTTCGCAAAACATTGCCATTCCCTGGTCTGGCGCAATATTTATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAGTCACGGGGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTTGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGa                                                                                                                                                                                                                                                   <  2:85908/238‑1 (MQ=255) tAAAGGCCAGCTATTCCGCATCGTTAATTATCCGCAGTTGGGATAAGCCCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGa                                                                                                                                                                                                                                                   <  1:949949/238‑1 (MQ=255) tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGa                                                                                                                                                                                                                                                   <  1:989127/238‑1 (MQ=255) tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGAAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGa                                                                                                                                                                                                                                                   <  1:822593/238‑1 (MQ=255) caaaGTCCGGGTATTCACCCTCGTTTATTCTCCGCAGTTGTTATTAGCGAAGTTTATTTCCAAAAACATTGCCCTTCCCTGCTTTGGCTCCAAATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTTATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGa                                                                                                                                                                                                                                                   <  2:970451/237‑1 (MQ=255)        cAGTTATTCTGCATGTTTAATTCTCCGCATTTGGGATAAGTCTAGTTTATTTCGCAAAACATTGCCATTCCCGGGTCTGGCGCAATATGCATTCTTATTGTCGCATTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGtaat                                                                                                                                                                                                                                            <  2:39052/238‑1 (MQ=255)        cAGTTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGtaat                                                                                                                                                                                                                                            <  1:677168/238‑1 (MQ=255)        cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGtaat                                                                                                                                                                                                                                            <  1:191506/238‑1 (MQ=255)        cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGtaat                                                                                                                                                                                                                                            <  1:599807/238‑1 (MQ=255)                  gtcttgTTAATTTGCCGCAGTTGTGCTAAGGGCAGTGTTTTTGCCAAAACATTGCCATTCCCTGCGCTGGCGCAATATTCATTCTTATTTTCGCGGTGATTCACGAACTGGTAAGTACATATTGACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑c                                                                                                                                                                                                                               <  2:416545/234‑1 (MQ=255)                   cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTGG‑ATTTTTT‑CC‑ATCCAAA‑ATAAAAAAT‑TG‑A‑GTTGTCATGAT‑TA‑GGGGAAGAAAAAAACAAAA‑gaac                                                                                                                                                                                                                               >  1:291498/1‑236 (MQ=255)                         tAATTTTCCGCCGTTGTGATAAGCGCCTTTTATTTCTCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AAt                                                                                                                                                                                                                       <  2:1247563/239‑1 (MQ=255)                         tAATTATCCCCAGTTGTGATAAGCGAAGTGTATTTCGGAAAACCATGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAGTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑aa                                                                                                                                                                                                                        <  1:77305/238‑1 (MQ=255)                                 cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑GATTTTT‑TC‑AAAAAAAAAAAAAAAAC‑GG‑A‑GATGTCATTAT‑GA‑GGGAAAGGAATAATAAACA‑GA‑CAAGG‑AATAAATTgtc                                                                                                                                                                                                               >  1:884001/1‑237 (MQ=255)                                      ttGTGTTAAGCTCGTTTTTTTTCCCAAAACTTTGCCATTCCCTTCTCTGGCGCAATATTCCTTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAgaga                                                                                                                                                                                                          <  2:153035/239‑1 (MQ=255)                                       tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAGTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGAt                                                                                                                                                                                                         <  1:319895/239‑1 (MQ=255)                                           aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATaa                                                                                                                                                                                                                     <  1:573673/223‑1 (MQ=255)                                                cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑TGACAAAAACAAAAAAT‑GG‑G‑GTTTGCCTGGT‑TA‑GGGGAAGTAATAAACAACA‑AG‑CAAGG‑GAAAAAATGACAGAGATTGTCGCT‑aa                                                                                                                                                                                               >  1:685558/1‑239 (MQ=255)                                                                 aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑CGACAAATACAAAAACA‑GG‑A‑GGTGCCCTGAT‑GA‑GCGGAAAGAATAAAAAACA‑AG‑CAGAG‑AAAAAGATGACAGAGATTGTGGCT‑AAC‑TTTACCGCCATGGttt                                                                                                                                                                             >  1:497336/1‑239 (MQ=255)                                                                 aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑CAACAAAATAAAAAACT‑GG‑G‑GTTTCCCTGGT‑TG‑TCGGAAGTAATAAACTAAA‑AG‑CAGGGAAAAAAGTGAACAGGAATTGTGCTT‑ACC‑TTTACCCCCagaatt                                                                                                                                                                              >  1:1320336/1‑234 (MQ=255)                                                                 aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AAATTTT‑TC‑ATACCAATAAAAAAAAT‑AT‑A‑GTTGCCCTGAT‑TA‑GCGGAAGTAAAAAGAAACA‑AG‑CAAGG‑AAAAATATGACAAAAGATGTTGCT‑CAC‑CTTACCTCCCTGAttt                                                                                                                                                                             >  1:851303/1‑239 (MQ=255)                                                                   cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGtaat                                                                                                                                                                                                                                            <  1:78684/179‑1 (MQ=255)                                                                         cATTCCCTGCTTGGGCGCAATTTTCATTCTTATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCAGGGTGGCAAAAAATAAAAACCCACATTTT‑T‑AGTG‑GTAGTTTGGGGCGGT‑G‑AATTTTT‑CC‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGa                                                                                                                                                                     <  1:31256/239‑1 (MQ=255)                                                                             cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑T‑AATATTT‑TT‑CGACCAAAACAAAAAAT‑GG‑A‑GTTGTCATGAT‑GT‑GGGGAAGAAAAAAACAAAC‑AC‑AAGTG‑AATAAGATTACAAAGATTGTTGCT‑AAT‑TTTATCCACCTCATTTCTTcccccaaca                                                                                                                                                                 >  1:1277090/1‑233 (MQ=255)                                                                                  ctctGGCGAAATATTCATTCTTATTTTCGCGTTGATGCAAGAACTGTTAATTACATATTCAGGGGGGAAAAAAATAAAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CA‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTAc                                                                                                                                                                         <  1:1451/226‑1 (MQ=255)                                                                                             tatTCTTTTTGATTTTCGGTTTGATTAACGAACTGTTAATTACATATTGACGGGTGCAAAAAATATAAAACCACATGTT‑G‑AGTG‑TTATTTGGTGGCTGT‑G‑AATTTGT‑CC‑AGACAAATACAAAAGCG‑GG‑A‑GTTGCCATGAT‑GA‑GGGAAAGTAATAAGCAACA‑GG‑AAGTG‑AAAAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                 <  1:1282646/239‑1 (MQ=255)                                                                                             tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                     <  1:857478/160‑1 (MQ=255)                                                                                                      ttATTTGCGGTTTGATTCACGAACTGTTAATTACATATTCCGGGGGGCAAAAAATAAAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATAAAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGAAAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                        <  1:1321586/239‑1 (MQ=255)                                                                                                       tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGACAAAAAATAAAAACCCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑A‑AATTTTC‑CC‑AGAAAAATAAAAAAACG‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  1:1155511/239‑1 (MQ=255)                                                                                                            tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑CGACAAATACAAAAAAA‑TG‑A‑GTTTCCATGAT‑GA‑GCGGAAGAAATAAAAAACA‑AG‑CAGGG‑AATAAAGTTTCAGAGATTTTCGCT‑TACATTTACC‑CCCTGGTTTTTATCCGAAAGCCTGATGACGTGGGGGGtaaaataaaaaaact                                                                                                                                  >  1:406976/1‑234 (MQ=255)                                                                                                                         cGATCTTTTAATTACATATTCAGGGTGACAAAAAATATAACACAACTTTTT‑T‑GGTG‑GTATTTGGTGGCGGT‑G‑ATTTTTC‑CC‑AGACAAATAAAAAAACT‑GG‑A‑TTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCTTT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                     <  1:131391/239‑1 (MQ=255)                                                                                                                                  aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                  <  1:499028/155‑1 (MQ=255)                                                                                                                                        atatTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑AGACAAATACAAAAAAA‑GG‑AGGTTGCC‑TGAT‑GA‑GGGAAAAGAAAAAACAAAA‑GG‑CAGAG‑AATAAGATGAAAGAGAGTTATGGT‑AAA‑TTTTCCGCCCTGAATTTTTCCCGAATGACTGCTGAAGTGGTGGGTGAAAAAAAACAACTAAAGGGTGCCGCAACAACGTCGTCgggg                                                                                                      >  1:836585/1‑239 (MQ=255)                                                                                                                                         ttttccgggTGGAAAAAAAAAAAAACCAAAATTGT‑T‑GGTG‑GTAGTTGGGGGGGGT‑A‑AATGTTA‑CA‑AGA‑AAAAAAAAAAAGT‑GGTG‑GTGGCCATGAA‑GA‑GCGAAATGAATAAG‑AACATGG‑CAGTG‑AATAAGTGGAAGGAGTTTGGCGTA‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:1320336/232‑1 (MQ=255)                                                                                                                                         ttgtCAGGGTAGAAAAAAATAAAAACCCAAATTTT‑A‑GGTG‑GTAGTTTGGGGCGTT‑G‑AATTTTA‑CA‑AGAAAAAAAAAAAACCG‑GG‑A‑GTTGCATTGAT‑GA‑GAAAAAGTAAAAAGCAACA‑GG‑AAGTA‑AATAATTTAAAAAAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:881361/236‑1 (MQ=255)                                                                                                                                         tatTCAAGTTGGCAAAAAATAAAAACCCACATTTT‑G‑AGGA‑GTA‑‑TTGTGGTGGA‑GTAATTTTTCCC‑AGAGAAATACAAAAACG‑GG‑A‑GTTGCTAGGAG‑GA‑GCGAAAGTACAAAGCACCA‑GG‑CAGAA‑AATAAGTTAACAGAGATGCTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:1316716/239‑1 (MQ=255)                                                                                                                                           ttCACGGTGACAAAAAATA‑AAAACCCAATGTT‑T‑GATGTGTAATTTGTGGCGGT‑A‑ATTCTCA‑AC‑AGAAAAAAAAAAAATGG‑GA‑A‑ATGGCAATGAT‑GT‑GCAAAAGAAAAAAGCAACA‑GG‑AATTG‑AATAAGTTGAAGGAGATTTAGTCT‑AAG‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:1260178/237‑1 (MQ=255)                                                                                                                                            tCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑AAACAAAAACAAAAACT‑GG‑T‑GTTTCCATGGT‑TA‑GGGGAAAAGATAAAAAACA‑GG‑CAAGG‑GATAAAATGTAAGAGATTGTTGCT‑CAC‑TTTTTCCCCCTGATTTCTACCCCCATGCCTGTTGAACTGGGGGAGAAACTAAAACAACTAAAAGAAGTCGAAAAGCCTCTGATGGGGgaaa                                                                                                  >  1:982406/1‑239 (MQ=255)                                                                                                                                              cggggggaaaaaaaTAT‑AAACCCCATTTT‑TAAGTG‑GTAGTTTGGGCAGGT‑G‑AATTTTT‑CA‑AGACAAATACAAAAACG‑GG‑A‑TTTGCCATGAT‑AA‑CGGAAAGAAAAAAGCAACG‑GG‑CAGAA‑AATAGGTTAACAGGGATTGGCCCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                <  1:263352/231‑1 (MQ=255)                                                                                                                                                ggTGGAAATAATTAAAAAACCATTTTTT‑T‑TGTG‑TTTGTTGCGGGTGGT‑T‑AATTTTT‑CC‑AGAAAAATGCAAAATGT‑GG‑A‑GTTGCCATGGT‑GG‑GGAAAAGTAATAAGCAAGA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  2:119881/239‑1 (MQ=255)                                                                                                                                                aaaagagaaataTAGAAACCCACATTTT‑G‑AGTG‑GTATTTTGGGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  1:99919/232‑1 (MQ=255)                                                                                                                                                 gTGGAAAAAAATA‑AAAAACACATTTTGT‑AGTG‑GTATTTTGGGGAGGA‑G‑AATTTGC‑CC‑AGAAAAAAAAAAAAACT‑GG‑A‑TTTGCCATGAT‑GA‑GCGAAAGTAAAAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTTTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                <  1:573467/236‑1 (MQ=255)                                                                                                                                                    gCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AAATTTT‑TC‑AAACAAAAAAAAAAAAT‑TG‑A‑GTTGCCCCGGT‑GA‑GCGGAAAGAATAAGCAAAA‑GG‑CAGGG‑AATAAGTTGAAAGAGAGTTTTGCT‑AAA‑TTTACCCCCCTGATTTTTACCCGCATTGCTGTGTACGTGGTGGGTAAACTTAAAAAACTCAAGGATAGCCAAACGTAGTCGATGGGGGAAAATATCTa                                                                                           >  1:948919/1‑238 (MQ=255)                                                                                                                                                    aaaaaaaaTAAAAACCCACTTTGT‑T‑GGGG‑TTATTTGGGGGCGGT‑A‑AATTTTT‑CA‑AAAAAAATAAAAAAACT‑GG‑G‑GTTGCCATGAT‑GA‑GGGAAAGTAATAAGCAACG‑GG‑CAGTA‑AATAAGTTGACAGAGATTGGCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                                                           <  1:249766/236‑1 (MQ=255)                                                                                                                                                          aaTATAAACCCCAATTGT‑T‑AGTG‑GTGATTTGGGTGGGT‑G‑AATTTTC‑CC‑AAAAAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GAGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGTTTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                    <  1:498943/239‑1 (MQ=255)                                                                                                                                                            aataAAACCCAATTTT‑TGAGGG‑GTATGTTGGGGGGGT‑G‑AATTTTC‑CC‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGa                                                                                   <  1:816851/238‑1 (MQ=255)                                                                                                                                                              aaaaaCCAGTTTTT‑A‑AGTG‑GTAGTTGGGGGGGGT‑A‑AATTTTC‑CA‑AGAAAAATAAAAAAACG‑GG‑A‑GTTGCAATGAG‑GA‑GAGAAAGTAAAAAGCAACG‑GG‑AAGTA‑AATAAGTGGAAAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGt                                                                                <  1:15825/238‑1 (MQ=255)                                                                                                                                                                  accatttttt‑t‑AG‑G‑GTGTTTTGTGGGCGG‑G‑AATGTTT‑CC‑AAAAAAAAAAAAAAAATGGG‑A‑GTGTCAATAGT‑GA‑GGAAAAATAATAAGC‑ACA‑GGTCAGTA‑AATAAG‑TAAAAGGGGTTTTCTCTAAAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGa                                                                            <  2:982406/233‑1 (MQ=255)                                                                                                                                                                   ccACATTTT‑T‑AGTG‑GTATTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGAAAAATAAAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCt                                                                                                                                                                           <  1:982238/143‑1 (MQ=255)                                                                                                                                                                                      atttttTGGCGGT‑G‑AATTTAT‑CA‑AGACAATATGACAAAGT‑GG‑G‑GTCACACTTAT‑GA‑GAGAAATTAAATGACAACA‑GG‑CAGTG‑AATAAATAGACAGGGATTGTCTAT‑TAT‑TTTTCCGCCCTGATTTCTCCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:1192264/237‑1 (MQ=255)                                                                                                                                                                                       tttttTGGGGGT‑T‑AAATTTT‑CCAAAAAAAAAACAAAAAAT‑GG‑A‑‑TTGGCATGAT‑AAGGCAAAAGAAATGAGAAACG‑GT‑CAGTA‑AATATGTAGAAAGAGATGGTCGCT‑CAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTCAAGGATGCCGAAACGTCGGCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:800417/238‑1 (MQ=255)                                                                                                                                                                                           gtgtCGGT‑G‑AATTGTT‑CC‑AAAAAAAAAAAAAAACG‑GG‑G‑GTTGGCA‑GATGGA‑GAGAAAGGAAAAACCAAAG‑GG‑GAGTA‑AATAAGTTGACGTGGATTGTCGCA‑AAT‑TTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                      <  2:234195/239‑1 (MQ=255)                                                                                                                                                                                                            tttcaaaaaaaaTAAAAAAACG‑GG‑A‑GTGGCCATGAT‑GA‑GCAAAAGTAAAAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                         <  1:1291857/230‑1 (MQ=255)                                                                                                                                                                                                               caaaaaaaaTAAAAAATTT‑GG‑A‑GTTGCCATGAT‑GA‑GCAAAAGTAATAAACAACA‑GG‑CAGTG‑AATAATTTGACAGAGTTGCCCGCT‑ACT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                      <  1:998544/233‑1 (MQ=255)                                                                                                                                                                                                               c‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                      >  1:737851/1‑238 (MQ=255)                                                                                                                                                                                                                      aaTACAAAAACT‑GG‑A‑TTTCCCATACG‑GG‑GCAAAAGTAATAAGCAACG‑GG‑CAGGG‑AATAGGTTGACAGGAATTGCCGCT‑ACC‑TTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                               <  1:624293/239‑1 (MQ=255)                                                                                                                                                                                                                             aaaCT‑GG‑A‑GTTGCCATGAT‑GA‑GCGACAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTCAAACAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCGCCCTGTCAgacacacggg                        >  2:350871/1‑230 (MQ=255)                                                                                                                                                                                                                              agcT‑GG‑A‑GTTGCCAAGAA‑GG‑GCAAAAGTAAAAACAAAAG‑GG‑CAGTA‑AATAAGTTGAAAGAAATTGCCGCT‑ATC‑GTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:750759/237‑1 (MQ=255)                                                                                                                                                                                                                               aagggg‑A‑GTTGCATTGAG‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGAT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:350871/235‑1 (MQ=255)                                                                                                                                                                                                                                    g‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCAAACGATGTTCGCCAACATGCAAAAAGGGATTGACTTGAATCGTCCTGCCTGTCAAGACACCGGGGGGAta                  >  2:1218750/1‑238 (MQ=255)                                                                                                                                                                                                                                           gCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGCGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGCAAATCATCTACCATACGATGTTCGACAACATGCAAAAAGCGCTTGACCTGAATCTTCCTGCCTGCCAGGACAACGGCAGGATTATGt              >  1:1179343/1‑238 (MQ=255)                                                                                                                                                                                                                                              atgat‑gA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGGGATTCTGttct           >  1:1098907/1‑238 (MQ=255)                                                                                                                                                                                                                                                        cGAAAGTAATAAGCAATA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTAGTCGATGGGGAAAATTATCTACCATACGAAGTTCTACAACAGCCAAAAAGCGATTGACCTGAATCATCCTGACTGCCGGGACACCGGGGGAATTATGTTCTTCGTTAAAg  >  2:867665/1‑239 (MQ=255)                                                                                                                                                                                                                                                        cGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAACGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTAACCTCAATCGCCCTGCCTGTCAGACAACCGGGGAGATTATGTTTTTCGTTAAAg  >  2:268898/1‑239 (MQ=255)                                                                                                                                                                                                                                                        cGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                               >  1:325370/1‑210 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206236‑3206709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑