Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,483	G→A	16.8%	K7K (AAG→AAA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,483	0	G	A	16.8%	111.9 / 4.2	61	K7K (AAG→AAA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (13/35);  new base A (8/2);  total (22/39)
Fisher's exact test for biased strand distribution p-value = 2.81e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGC  >  NC_000913/3206253‑3206720                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   gtcttgTTAATTTGCCGCAGTTGTGCTAAGGGCAGTGTTTTTGCCAAAACATTGCCATTCCCTGCGCTGGCGCAATATTCATTCTTATTTTCGCGGTGATTCACGAACTGGTAAGTACATATTGACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑c                                                                                                                                                                                                                                          <  2:416545/234‑1 (MQ=255)  cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTGG‑ATTTTTT‑CC‑ATCCAAA‑ATAAAAAAT‑TG‑A‑GTTGTCATGAT‑TA‑GGGGAAGAAAAAAACAAAA‑gaac                                                                                                                                                                                                                                          >  1:291498/1‑236 (MQ=255)        tAATTTTCCGCCGTTGTGATAAGCGCCTTTTATTTCTCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AAt                                                                                                                                                                                                                                  <  2:1247563/239‑1 (MQ=255)        tAATTATCCCCAGTTGTGATAAGCGAAGTGTATTTCGGAAAACCATGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAGTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑aa                                                                                                                                                                                                                                   <  1:77305/238‑1 (MQ=255)                cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑GATTTTT‑TC‑AAAAAAAAAAAAAAAAC‑GG‑A‑GATGTCATTAT‑GA‑GGGAAAGGAATAATAAACA‑GA‑CAAGG‑AATAAATTgtc                                                                                                                                                                                                                          >  1:884001/1‑237 (MQ=255)                     ttGTGTTAAGCTCGTTTTTTTTCCCAAAACTTTGCCATTCCCTTCTCTGGCGCAATATTCCTTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAgaga                                                                                                                                                                                                                     <  2:153035/239‑1 (MQ=255)                      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAGTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGAt                                                                                                                                                                                                                    <  1:319895/239‑1 (MQ=255)                          aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATaa                                                                                                                                                                                                                                <  1:573673/223‑1 (MQ=255)                               cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑TGACAAAAACAAAAAAT‑GG‑G‑GTTTGCCTGGT‑TA‑GGGGAAGTAATAAACAACA‑AG‑CAAGG‑GAAAAAATGACAGAGATTGTCGCT‑aa                                                                                                                                                                                                          >  1:685558/1‑239 (MQ=255)                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑CGACAAATACAAAAACA‑GG‑A‑GGTGCCCTGAT‑GA‑GCGGAAAGAATAAAAAACA‑AG‑CAGAG‑AAAAAGATGACAGAGATTGTGGCT‑AAC‑TTTACCGCCATGGttt                                                                                                                                                                                        >  1:497336/1‑239 (MQ=255)                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑CAACAAAATAAAAAACT‑GG‑G‑GTTTCCCTGGT‑TG‑TCGGAAGTAATAAACTAAA‑AG‑CAGGGAAAAAAGTGAACAGGAATTGTGCTT‑ACC‑TTTACCCCCagaatt                                                                                                                                                                                         >  1:1320336/1‑234 (MQ=255)                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AAATTTT‑TC‑ATACCAATAAAAAAAAT‑AT‑A‑GTTGCCCTGAT‑TA‑GCGGAAGTAAAAAGAAACA‑AG‑CAAGG‑AAAAATATGACAAAAGATGTTGCT‑CAC‑CTTACCTCCCTGAttt                                                                                                                                                                                        >  1:851303/1‑239 (MQ=255)                                                        cATTCCCTGCTTGGGCGCAATTTTCATTCTTATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCAGGGTGGCAAAAAATAAAAACCCACATTTT‑T‑AGTG‑GTAGTTTGGGGCGGT‑G‑AATTTTT‑CC‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGa                                                                                                                                                                                <  1:31256/239‑1 (MQ=255)                                                            cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑T‑AATATTT‑TT‑CGACCAAAACAAAAAAT‑GG‑A‑GTTGTCATGAT‑GT‑GGGGAAGAAAAAAACAAAC‑AC‑AAGTG‑AATAAGATTACAAAGATTGTTGCT‑AAT‑TTTATCCACCTCATTTCTTcccccaaca                                                                                                                                                                            >  1:1277090/1‑233 (MQ=255)                                                                 ctctGGCGAAATATTCATTCTTATTTTCGCGTTGATGCAAGAACTGTTAATTACATATTCAGGGGGGAAAAAAATAAAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CA‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTAc                                                                                                                                                                                    <  1:1451/226‑1 (MQ=255)                                                                            tatTCTTTTTGATTTTCGGTTTGATTAACGAACTGTTAATTACATATTGACGGGTGCAAAAAATATAAAACCACATGTT‑G‑AGTG‑TTATTTGGTGGCTGT‑G‑AATTTGT‑CC‑AGACAAATACAAAAGCG‑GG‑A‑GTTGCCATGAT‑GA‑GGGAAAGTAATAAGCAACA‑GG‑AAGTG‑AAAAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  1:1282646/239‑1 (MQ=255)                                                                            tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                                <  1:857478/160‑1 (MQ=255)                                                                                     ttATTTGCGGTTTGATTCACGAACTGTTAATTACATATTCCGGGGGGCAAAAAATAAAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATAAAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGAAAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                   <  1:1321586/239‑1 (MQ=255)                                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGACAAAAAATAAAAACCCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑A‑AATTTTC‑CC‑AGAAAAATAAAAAAACG‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                  <  1:1155511/239‑1 (MQ=255)                                                                                           tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑CGACAAATACAAAAAAA‑TG‑A‑GTTTCCATGAT‑GA‑GCGGAAGAAATAAAAAACA‑AG‑CAGGG‑AATAAAGTTTCAGAGATTTTCGCT‑TACATTTACC‑CCCTGGTTTTTATCCGAAAGCCTGATGACGTGGGGGGtaaaataaaaaaact                                                                                                                                             >  1:406976/1‑234 (MQ=255)                                                                                                        cGATCTTTTAATTACATATTCAGGGTGACAAAAAATATAACACAACTTTTT‑T‑GGTG‑GTATTTGGTGGCGGT‑G‑ATTTTTC‑CC‑AGACAAATAAAAAAACT‑GG‑A‑TTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCTTT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                                <  1:131391/239‑1 (MQ=255)                                                                                                                 aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                             <  1:499028/155‑1 (MQ=255)                                                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑AGACAAATACAAAAAAA‑GG‑AGGTTGCC‑TGAT‑GA‑GGGAAAAGAAAAAACAAAA‑GG‑CAGAG‑AATAAGATGAAAGAGAGTTATGGT‑AAA‑TTTTCCGCCCTGAATTTTTCCCGAATGACTGCTGAAGTGGTGGGTGAAAAAAAACAACTAAAGGGTGCCGCAACAACGTCGTCgggg                                                                                                                 >  1:836585/1‑239 (MQ=255)                                                                                                                        ttttccgggTGGAAAAAAAAAAAAACCAAAATTGT‑T‑GGTG‑GTAGTTGGGGGGGGT‑A‑AATGTTA‑CA‑AGA‑AAAAAAAAAAAGT‑GGTG‑GTGGCCATGAA‑GA‑GCGAAATGAATAAG‑AACATGG‑CAGTG‑AATAAGTGGAAGGAGTTTGGCGTA‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                <  2:1320336/232‑1 (MQ=255)                                                                                                                        ttgtCAGGGTAGAAAAAAATAAAAACCCAAATTTT‑A‑GGTG‑GTAGTTTGGGGCGTT‑G‑AATTTTA‑CA‑AGAAAAAAAAAAAACCG‑GG‑A‑GTTGCATTGAT‑GA‑GAAAAAGTAAAAAGCAACA‑GG‑AAGTA‑AATAATTTAAAAAAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                <  1:881361/236‑1 (MQ=255)                                                                                                                        tatTCAAGTTGGCAAAAAATAAAAACCCACATTTT‑G‑AGGA‑GTA‑‑TTGTGGTGGA‑GTAATTTTTCCC‑AGAGAAATACAAAAACG‑GG‑A‑GTTGCTAGGAG‑GA‑GCGAAAGTACAAAGCACCA‑GG‑CAGAA‑AATAAGTTAACAGAGATGCTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                <  1:1316716/239‑1 (MQ=255)                                                                                                                          ttCACGGTGACAAAAAATA‑AAAACCCAATGTT‑T‑GATGTGTAATTTGTGGCGGT‑A‑ATTCTCA‑AC‑AGAAAAAAAAAAAATGG‑GA‑A‑ATGGCAATGAT‑GT‑GCAAAAGAAAAAAGCAACA‑GG‑AATTG‑AATAAGTTGAAGGAGATTTAGTCT‑AAG‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                <  1:1260178/237‑1 (MQ=255)                                                                                                                           tCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑TC‑AAACAAAAACAAAAACT‑GG‑T‑GTTTCCATGGT‑TA‑GGGGAAAAGATAAAAAACA‑GG‑CAAGG‑GATAAAATGTAAGAGATTGTTGCT‑CAC‑TTTTTCCCCCTGATTTCTACCCCCATGCCTGTTGAACTGGGGGAGAAACTAAAACAACTAAAAGAAGTCGAAAAGCCTCTGATGGGGgaaa                                                                                                             >  1:982406/1‑239 (MQ=255)                                                                                                                             cggggggaaaaaaaTAT‑AAACCCCATTTT‑TAAGTG‑GTAGTTTGGGCAGGT‑G‑AATTTTT‑CA‑AGACAAATACAAAAACG‑GG‑A‑TTTGCCATGAT‑AA‑CGGAAAGAAAAAAGCAACG‑GG‑CAGAA‑AATAGGTTAACAGGGATTGGCCCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                           <  1:263352/231‑1 (MQ=255)                                                                                                                               ggTGGAAATAATTAAAAAACCATTTTTT‑T‑TGTG‑TTTGTTGCGGGTGGT‑T‑AATTTTT‑CC‑AGAAAAATGCAAAATGT‑GG‑A‑GTTGCCATGGT‑GG‑GGAAAAGTAATAAGCAAGA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                         <  2:119881/239‑1 (MQ=255)                                                                                                                               aaaagagaaataTAGAAACCCACATTTT‑G‑AGTG‑GTATTTTGGGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                         <  1:99919/232‑1 (MQ=255)                                                                                                                                gTGGAAAAAAATA‑AAAAACACATTTTGT‑AGTG‑GTATTTTGGGGAGGA‑G‑AATTTGC‑CC‑AGAAAAAAAAAAAAACT‑GG‑A‑TTTGCCATGAT‑GA‑GCGAAAGTAAAAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTTTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                           <  1:573467/236‑1 (MQ=255)                                                                                                                                   gCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AAATTTT‑TC‑AAACAAAAAAAAAAAAT‑TG‑A‑GTTGCCCCGGT‑GA‑GCGGAAAGAATAAGCAAAA‑GG‑CAGGG‑AATAAGTTGAAAGAGAGTTTTGCT‑AAA‑TTTACCCCCCTGATTTTTACCCGCATTGCTGTGTACGTGGTGGGTAAACTTAAAAAACTCAAGGATAGCCAAACGTAGTCGATGGGGGAAAATATCTa                                                                                                      >  1:948919/1‑238 (MQ=255)                                                                                                                                   aaaaaaaaTAAAAACCCACTTTGT‑T‑GGGG‑TTATTTGGGGGCGGT‑A‑AATTTTT‑CA‑AAAAAAATAAAAAAACT‑GG‑G‑GTTGCCATGAT‑GA‑GGGAAAGTAATAAGCAACG‑GG‑CAGTA‑AATAAGTTGACAGAGATTGGCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                                                                      <  1:249766/236‑1 (MQ=255)                                                                                                                                         aaTATAAACCCCAATTGT‑T‑AGTG‑GTGATTTGGGTGGGT‑G‑AATTTTC‑CC‑AAAAAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GAGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGTTTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                               <  1:498943/239‑1 (MQ=255)                                                                                                                                           aataAAACCCAATTTT‑TGAGGG‑GTATGTTGGGGGGGT‑G‑AATTTTC‑CC‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGa                                                                                              <  1:816851/238‑1 (MQ=255)                                                                                                                                             aaaaaCCAGTTTTT‑A‑AGTG‑GTAGTTGGGGGGGGT‑A‑AATTTTC‑CA‑AGAAAAATAAAAAAACG‑GG‑A‑GTTGCAATGAG‑GA‑GAGAAAGTAAAAAGCAACG‑GG‑AAGTA‑AATAAGTGGAAAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGt                                                                                           <  1:15825/238‑1 (MQ=255)                                                                                                                                                 accatttttt‑t‑AG‑G‑GTGTTTTGTGGGCGG‑G‑AATGTTT‑CC‑AAAAAAAAAAAAAAAATGGG‑A‑GTGTCAATAGT‑GA‑GGAAAAATAATAAGC‑ACA‑GGTCAGTA‑AATAAG‑TAAAAGGGGTTTTCTCTAAAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGa                                                                                       <  2:982406/233‑1 (MQ=255)                                                                                                                                                  ccACATTTT‑T‑AGTG‑GTATTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGAAAAATAAAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCt                                                                                                                                                                                      <  1:982238/143‑1 (MQ=255)                                                                                                                                                                     atttttTGGCGGT‑G‑AATTTAT‑CA‑AGACAATATGACAAAGT‑GG‑G‑GTCACACTTAT‑GA‑GAGAAATTAAATGACAACA‑GG‑CAGTG‑AATAAATAGACAGGGATTGTCTAT‑TAT‑TTTTCCGCCCTGATTTCTCCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                      <  1:1192264/237‑1 (MQ=255)                                                                                                                                                                      tttttTGGGGGT‑T‑AAATTTT‑CCAAAAAAAAAACAAAAAAT‑GG‑A‑‑TTGGCATGAT‑AAGGCAAAAGAAATGAGAAACG‑GT‑CAGTA‑AATATGTAGAAAGAGATGGTCGCT‑CAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTCAAGGATGCCGAAACGTCGGCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                      <  2:800417/238‑1 (MQ=255)                                                                                                                                                                          gtgtCGGT‑G‑AATTGTT‑CC‑AAAAAAAAAAAAAAACG‑GG‑G‑GTTGGCA‑GATGGA‑GAGAAAGGAAAAACCAAAG‑GG‑GAGTA‑AATAAGTTGACGTGGATTGTCGCA‑AAT‑TTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                                 <  2:234195/239‑1 (MQ=255)                                                                                                                                                                                           tttcaaaaaaaaTAAAAAAACG‑GG‑A‑GTGGCCATGAT‑GA‑GCAAAAGTAAAAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                    <  1:1291857/230‑1 (MQ=255)                                                                                                                                                                                              caaaaaaaaTAAAAAATTT‑GG‑A‑GTTGCCATGAT‑GA‑GCAAAAGTAATAAACAACA‑GG‑CAGTG‑AATAATTTGACAGAGTTGCCCGCT‑ACT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                                 <  1:998544/233‑1 (MQ=255)                                                                                                                                                                                              c‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                                 >  1:737851/1‑238 (MQ=255)                                                                                                                                                                                                     aaTACAAAAACT‑GG‑A‑TTTCCCATACG‑GG‑GCAAAAGTAATAAGCAACG‑GG‑CAGGG‑AATAGGTTGACAGGAATTGCCGCT‑ACC‑TTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                          <  1:624293/239‑1 (MQ=255)                                                                                                                                                                                                            aaaCT‑GG‑A‑GTTGCCATGAT‑GA‑GCGACAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTCAAACAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCGCCCTGTCAgacacacggg                                   >  2:350871/1‑230 (MQ=255)                                                                                                                                                                                                             agcT‑GG‑A‑GTTGCCAAGAA‑GG‑GCAAAAGTAAAAACAAAAG‑GG‑CAGTA‑AATAAGTTGAAAGAAATTGCCGCT‑ATC‑GTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                  <  1:750759/237‑1 (MQ=255)                                                                                                                                                                                                              aagggg‑A‑GTTGCATTGAG‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGAT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                  <  1:350871/235‑1 (MQ=255)                                                                                                                                                                                                                   g‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCAAACGATGTTCGCCAACATGCAAAAAGGGATTGACTTGAATCGTCCTGCCTGTCAAGACACCGGGGGGAta                             >  2:1218750/1‑238 (MQ=255)                                                                                                                                                                                                                          gCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGCGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGCAAATCATCTACCATACGATGTTCGACAACATGCAAAAAGCGCTTGACCTGAATCTTCCTGCCTGCCAGGACAACGGCAGGATTATGt                         >  1:1179343/1‑238 (MQ=255)                                                                                                                                                                                                                             atgat‑gA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGGGATTCTGttct                      >  1:1098907/1‑238 (MQ=255)                                                                                                                                                                                                                                       cGAAAGTAATAAGCAATA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTAGTCGATGGGGAAAATTATCTACCATACGAAGTTCTACAACAGCCAAAAAGCGATTGACCTGAATCATCCTGACTGCCGGGACACCGGGGGAATTATGTTCTTCGTTAAAg             >  2:867665/1‑239 (MQ=255)                                                                                                                                                                                                                                       cGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAACGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTAACCTCAATCGCCCTGCCTGTCAGACAACCGGGGAGATTATGTTTTTCGTTAAAg             >  2:268898/1‑239 (MQ=255)                                                                                                                                                                                                                                       cGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                          >  1:325370/1‑210 (MQ=255)                                                                                                                                                                                                                                           aGTAATAAGCACCA‑GG‑CAGTG‑AATAAGTTAACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                               <  2:187389/73‑1 (MQ=255)                                                                                                                                                                                                                                           aGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                               >  1:187389/1‑73 (MQ=255)                                                                                                                                                                                                                                           aGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg         >  1:192115/1‑239 (MQ=255)                                                                                                                                                                                                                                              aataaGCAACG‑GG‑GGTTA‑AATATTTTGATAGAGTTTTCCGTT‑ATA‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc      <  2:685558/239‑1 (MQ=255)                                                                                                                                                                                                                                                taaGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGACAGAAAACCGGAGAGATTATGTTCTTCGTTAAAGTAGGTGccc    >  2:494328/1‑239 (MQ=255)                                                                                                                                                                                                                                                  aGCAACG‑GT‑GAGTA‑AATAATGTGACAGAGATGGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGc  <  1:1211192/239‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   GCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTT‑T‑AGTG‑GTAGTTTGTGGCGGT‑G‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCATGAT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGC  >  NC_000913/3206253‑3206720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑