Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,480,296 T→C 21.1% G104G (GGA→GGG kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,480,2960TC21.1% 169.1 / 6.0 76G104G (GGA→GGGkefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (48/8);  new base C (2/16);  total (50/26)
Fisher's exact test for biased strand distribution p-value = 1.26e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAACACAGCGC  >  NC_000913/3480060‑3480519
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 
aCCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCCGTTGAAGACATCGCAAGGCCAATGCCACCAACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAAAAATc                                                                                                                                                                                                                                 >  1:984138/1‑238 (MQ=255)
aCCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCACCATCAATAATc                                                                                                                                                                                                                                 >  1:947338/1‑238 (MQ=255)
aCCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCAAGCGGAAATCCGTCAGCATCAATAATc                                                                                                                                                                                                                                 >  1:44162/1‑238 (MQ=255)
      aTCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCa                                                                                                                                                                                                                           >  1:587663/1‑238 (MQ=255)
            tCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGCCCACCGCCGCCTGCCAGGCGAAACCCGCCAGCATCAATAATCCCGCCAGCAAcg                                                                                                                                                                                                                     >  2:813364/1‑238 (MQ=255)
            tCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAAcg                                                                                                                                                                                                                     >  1:1046092/1‑238 (MQ=255)
                          tGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCCACGCCATTGCAGTTGAAGCCATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAAACCGTCATCATCAATAATCCCGCCAGCAACCCCGCGCTTAAAAga                                                                                                                                                                                                       >  1:485755/1‑237 (MQ=255)
                                   cGCCAACAACGGCACTAACGCCAGTGCTGGGCTTACTGCCAGATCCTGAAACAGCAGCACCGACAACCCGAGCTGGCCTGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATGGCAATGCCAATGCCACCGCCCACCGCCGCCTGCCAGGCCAAATCCGCCCGCATCCATACTCCCGCCTGCCACCACGCGCTTACAGCACCCTGTGcc                                                                                                                                                                                             >  2:802850/1‑238 (MQ=255)
                                   cGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGCCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGcc                                                                                                                                                                                             >  1:1073417/1‑238 (MQ=255)
                                               cACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGccca                                                                                                                                                                                >  1:689662/1‑237 (MQ=255)
                                               cACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACCCCaa                                                                                                                                                                                >  1:850619/1‑239 (MQ=255)
                                               cACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAACCCCGCCAGCAACCCCGCGCTTAACAGCACCCTTACCGCGCCCACCCCaa                                                                                                                                                                                >  2:750085/1‑239 (MQ=255)
                                                         aGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCCCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCCGCAACCCCCCGCTTAACACCCCCTGTCCCCCCCCTCCCCCaaaaacggaag                                                                                                                                                                       >  2:871708/1‑232 (MQ=255)
                                                         aGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCCCCTGCCAGGCGAAAACCGTCCGCCTCCATAATCCCCCCAGCAACCCCCCCCTATAAACAACCCGTCCCCCGCCCACCACAAAAACCGaaa                                                                                                                                                                       >  2:871707/1‑237 (MQ=255)
                                                               gggATTACTGCCAGATCCGAAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGGGATTCATCCCTTTCCCCCCCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCa                                                                                                                                                                 <  1:1106405/238‑1 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGCAATCCGTCAGCATCAATAACCCCGCCCGCAACGCCCCGCTTAACACCACCCGTGCCCCCCCTCCCCCAAAAAAACAACGCCGCaatggcaa                                                                                                                                                          >  2:1277304/1‑232 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTaa                                                                                                                                                                                                           >  1:1139896/1‑191 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCa                                                                                                                                                          >  1:913652/1‑239 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCACTTGCCa                                                                                                                                                          >  1:1005936/1‑239 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACACCGCAGTTGccc                                                                                                                                                          >  1:1098079/1‑238 (MQ=255)
                                                                            gATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTAAAGCATCAATTGCAACGCCATTGCAGTTGAAGACATCCCAAGGCCAATGCCCCCGACCACCGCACCCTGCGAGGCGAAATCCGTCAGCATCAAAAATCCCGCACCCAACGCCGCGCTTCAACGCACCTGTGCCGCCCCAACCCCAGAAAACGAACCCCTCACttcgaaaaatgtg                                                                                                                                                   >  2:1074367/1‑228 (MQ=255)
                                                                                   aaaCAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGAt                                                                                                                                            >  1:855497/1‑239 (MQ=255)
                                                                                    aaCAGCAAACCCGAAAGCCCGAGCTGGCCGGATTCGCTGCGATTCACCCCTTTCCCCCGCATCAATTGCACCGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGAtt                                                                                                                                           <  1:413310/239‑1 (MQ=255)
                                                                                         cagAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAAc                                                                                                                                       >  1:1254538/1‑238 (MQ=255)
                                                                                         cagAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACCCCGCAGTTGCCAAAGTTTGGAGGGATTCAAc                                                                                                                                       >  1:201924/1‑238 (MQ=255)
                                                                                          caaccacaaaaCCCGGGGTGGCCGGTTTCTGTCGTATTCATCCCCTTCCCACCCACCAATTGCAACCCCATTAGAGTGGAAACCACCGCAAGCCAAATGCCACCGCCCCCCGCCGCCTGCAGGGCGAAATCCGCCAGAATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTc                                                                                                                                     <  2:587663/232‑1 (MQ=255)
                                                                                           gAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGACGTAGCCAAAGATTGGAGGGATTCAACTCa                                                                                                                                    >  1:876977/1‑239 (MQ=255)
                                                                                               ccaaacCCCTAGCTGGCGGGATTCGCGTCGTTCCACCCCTTTCTCCCGCTCACATTGCAACCCCGTTGACGTGGAAGACCTCGCAAGGCCCATCCCCCCGACCACCGCCGCCCGCCAGGCAAACTCCGTCAGCATCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGc                                                                                                                                <  2:947338/237‑1 (MQ=255)
                                                                                                              gCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCCCAGTTGCCAAAGTTTGGCGGGGTTCAACTCAGGGCCGTTGATAAACATCa                                                                                                                 >  1:1080270/1‑239 (MQ=255)
                                                                                                                     tCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATa                                                                                                           >  1:701213/1‑238 (MQ=255)
                                                                                                                             aTTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCCCCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCcgcg                                                                                                                                                                                                                <  1:950134/130‑1 (MQ=255)
                                                                                                                             aTTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCcgcg                                                                                                                                                                                                                >  2:950134/1‑130 (MQ=255)
                                                                                                                                      tttcccccccATCAATTCCAACCCACTTGCAGTTGAAGACACCCCAAGGCCAATGCCCCCCACCGCCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGcaa                                                                                         <  1:715009/230‑1 (MQ=255)
                                                                                                                                        tctcACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGACCGCCGCAGTTGCCAAAGTTTGGAGGGCTTCAACTCAGGGCCGTTGCTAAACATCAGGAATACCACGCCGAGTTCCGaaaaa                                                                                       >  1:939300/1‑238 (MQ=255)
                                                                                                                                        tctcACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCACCTCAAGGCCGAGGATAAACATCAGGAATACCACCCCGAGTTCCGaaact                                                                                       >  1:680983/1‑237 (MQ=255)
                                                                                                                                        tctcACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAAc                                                                                                                                       >  1:586798/1‑191 (MQ=255)
                                                                                                                                                ataaaTTGCAAGCCCATTGCAATTGAAGAAAACGAAGGCCCACGGCCCCCGCCCACCGCCGCCGGCCAGGCGAAATCCGGCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGAt                                                                               <  1:556841/236‑1 (MQ=255)
                                                                                                                                                     aTTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCCAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggaggactcca                                                                           >  1:1034435/1‑234 (MQ=255)
                                                                                                                                                           aCGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGCCg                                                                     >  1:840980/1‑239 (MQ=255)
                                                                                                                                                           aCGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGGGGAGGATCTCATCGAc                                                                      >  1:601369/1‑238 (MQ=255)
                                                                                                                                                            ccccATTGGAGTTAAAGACCTCCGAAGGCCAACGCCCCCGCCCCCCGCCGCCTGCCGGGCAAAATCCGCAAACAACAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGt                                                                    <  1:871708/237‑1 (MQ=255)
                                                                                                                                                            cGCCATTGCAGTTGAAAAAAACACAAGGACAATGCCCCCGCCCCCCGCACCCGGCCAGGCAAAACCCGCCAGACTCAATATACCCGCCAGCAACGCCGCGCTTAACAGCACCTGGGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGt                                                                    <  1:871707/239‑1 (MQ=255)
                                                                                                                                                              ccATTGCAGTTGAAGACATCGAAAGGCCAATGCCACCGACCCCCGCCGCCTGCCGGGCAAAACCCGTCAGCATAAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCg                                                                  <  1:813364/239‑1 (MQ=255)
                                                                                                                                                                 ttGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGCGGATCTCATCGACGCCCCta                                                               >  1:885625/1‑239 (MQ=255)
                                                                                                                                                                  tGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGCGTTCCGAAAAGTGGGGGATATCATCGACGTCGCtaa                                                              >  1:1071989/1‑239 (MQ=255)
                                                                                                                                                                         gAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGGTCCCATCGACGTCGCTAATAAAcc                                                        >  1:1208165/1‑238 (MQ=255)
                                                                                                                                                                         gAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGAACTCATCGACGTCCCTAATAAAccc                                                       >  1:80233/1‑239 (MQ=255)
                                                                                                                                                                         gAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAAAATCAGGAATACCACGCCGAGTTCCGAAAATGGGAGGATCTCATCGACGTCGCTAATAAAcc                                                        >  1:1021092/1‑238 (MQ=255)
                                                                                                                                                                              cATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAAAAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCCGTTGCCCAAGTTTGGCGGGATTAAACTCAAAGCAGATGCTAAACCTCAGGAATACCACGCCGCGTTCCGAAACGTGGAGGATCACCTCGACGTCGCTAAAAAACCCCacc                                                   >  2:213925/1‑236 (MQ=255)
                                                                                                                                                                              aaccacAAGGCAAAGCCCACCGACCCCCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAg                                                    <  1:750085/232‑1 (MQ=255)
                                                                                                                                                                               accGCAAGGCAAATGCACCCGCCCCCCGCCCCCTGCCAGGCGAAATCCGTCAGCAACAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGc                                                   <  1:213288/235‑1 (MQ=255)
                                                                                                                                                                                 cGCCAGGCCAATGCCACCGCCCCCCGCCCCCTGCCAGGCAAAACCCGTCAGCACCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGccccc                                               <  1:430386/239‑1 (MQ=255)
                                                                                                                                                                                 cGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGCGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCCCGCCGAGTTCCGAAAAGAGGGGGGTCCCATACACGTCCCTAAAAAACCCAAGccccc                                               >  2:213288/1‑239 (MQ=255)
                                                                                                                                                                                 cGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCATACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTAAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGATCCGAAAAGCGGAGGAGCTCATCGACGTCGATAATAAACCCCCAcccc                                                >  1:345040/1‑238 (MQ=255)
                                                                                                                                                                                 cGCAAAGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCACTAATCCCGCCAGCAACCCCCCGCTTAACAGCACCTTTGCCGCGCCTACGCCAAAAATCGACCTCCTCAGTTGCCAAAGTTTGCAGGGATCCACCTCAAGGCCGACGATAAACACTCGGAATACCTCCCCGGGTTCCCCAACGGGAGGGATCCCATCCCCACCGCTAAAAAAccaccgaaccc                                               >  2:1127622/1‑230 (MQ=255)
                                                                                                                                                                                  gCAAGGCCAATGCCACCGCCCCCCCCCCCCTGCCAGTCAAAACCCGTCAGCATAAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCa                                              <  1:1252060/239‑1 (MQ=255)
                                                                                                                                                                                   cAAGGCCAATCCCACCGACCCCCGCCGCCGGCCAGGCAAAATCCTGCAGCATCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCAc                                             <  1:213925/239‑1 (MQ=255)
                                                                                                                                                                                    aaGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCCACTCAAGGCCGATGATAAACATCAGGAAACCCACGCCGAGTTCCGAAAAGAGGAGGATTCCCCCGACGTCGCCAACAAACCCAAGCCCCCACg                                            >  2:430386/1‑239 (MQ=255)
                                                                                                                                                                                       cccaacccccccGCCCCCCCCCCCCTGCCTGGCAAACTCCCTCAGCCTAAAAAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGc                                         <  2:913652/229‑1 (MQ=255)
                                                                                                                                                                                            tGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAAtt                                    >  1:1179574/1‑239 (MQ=255)
                                                                                                                                                                                                 ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGGGTTCCGAAAAGTGGAGAACCTCATCGCCGCCGCAAATAAACCCCACCCCCCACGGCCCAATTGCaa                                >  2:190004/1‑238 (MQ=255)
                                                                                                                                                                                                 ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTCCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCaa                                >  1:935678/1‑238 (MQ=255)
                                                                                                                                                                                                  cGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAACGTGGAGGATATCATCGACGTCGCTAATAAACCCCAACACCCACGGGCCAATTGCAAt                               >  1:84339/1‑238 (MQ=255)
                                                                                                                                                                                                    ccccccgccgCCCGCCAGGCGAAACCCGTCAGCATAAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATccc                            <  1:449250/235‑1 (MQ=255)
                                                                                                                                                                                                              ccggccagcgcaaaTCCGACAGCATCAAATACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:464699/228‑1 (MQ=255)
                                                                                                                                                                                                              cccaccgggCAAAACCCGTAAGCTACAAAAGCCCCGCCAGCAACGCCGCGCTTAACAGCACCGGTGCCGCGCATACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATCCCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:1074367/232‑1 (MQ=255)
                                                                                                                                                                                                              ccTGCCGGGCAAATGCCGCCAAAATCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:66661/239‑1 (MQ=255)
                                                                                                                                                                                                              ccTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGCTAAACATCAGGAATACCACGCCAAGTTCCGAAAACTGGAGGAGCACATCGACGTCACAAATCAACCCCCACCACCACGGGACACATGCAAACCCCGCCACCaaa                  >  2:640201/1‑239 (MQ=255)
                                                                                                                                                                                                              ccTGCCAGGCCAACTCCGCCAGCCTCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:1227873/239‑1 (MQ=255)
                                                                                                                                                                                                              ccTGACAGGCGAAACCCGGCAGCATCAATAACCCCGCAAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGGTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:802850/239‑1 (MQ=255)
                                                                                                                                                                                                                   cagggcaaaTCGGTAAGCATCAAAAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATcc             <  1:260506/233‑1 (MQ=255)
                                                                                                                                                                                                                      gCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCACAAATCGAACGCCTCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGCTGATAAACATCCGGAATACCACGCCTGGTTCCGCAAATGGGAGCACCCACTCGCCGTCGCCAATAAACCCCCGCCCCCCCCGGCCAACTGCAAACCCCGCGGCCAAAAATCCaaa          >  2:99260/1‑239 (MQ=255)
                                                                                                                                                                                                                      gCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGCGGGATTCAACTCAAGACCGATGCTAAACATCAGGAATACCACGCCCAGTTCCGAAAAGTGGAGGATCTCATCGACGCCGCCAAAAAACCCCCACCCCCACCGGCCAAATGCCATCCACGCCCCCAAACATCCCa           >  2:87665/1‑238 (MQ=255)
                                                                                                                                                                                                                          aaTCCGCAAGCACAATAAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAAcaca      <  1:1130584/239‑1 (MQ=255)
                                                                                                                                                                                                                           aTCCGTCAGCTACAAAACCCCCGCCAGCAACCCCGCCACTAACAGCCCCTGGGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAACACAg     <  1:616446/239‑1 (MQ=255)
                                                                                                                                                                                                                              cGTCAGCAACAATAACCCCGCCAGCAACGCCGCGGTTAACAGCCCCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAACACAgcgc  <  1:692971/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 
ACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCAATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAACACAGCGC  >  NC_000913/3480060‑3480519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: