Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,499,694 G→T 11.7% intergenic (+79/‑216) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,499,6940GT11.7% 206.6 / 2.1 74intergenic (+79/‑216)yhfL/frlAsmall lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (21/43);  new base T (7/3);  total (28/46)
Fisher's exact test for biased strand distribution p-value = 3.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGG  >  NC_000913/3499475‑3499914
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                
gTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGGCCTACTGCACACAAAATCTCTTACCGAAAACGGCTAACACCCGTTTATATTTCCGCCACCCTCTGGGTTTTTCCCGTGCGCGTTTTTTTCTCTGTGCTTCTTT‑‑Ccccccccc                                                                                                                                                                                                             >  1:1269312/1‑238 (MQ=255)
     tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCACTGCCCAAAAAATCTCTTACAGAAAATCGGTCACACCCGGTTTATTGCCCCCCGCCCCCCTGAATTTCCGAGGGCGGCTTTTTTTCTTTGTCCCTCTTT‑‑Ccccccccccccaaa                                                                                                                                                                                                       >  1:498428/1‑233 (MQ=255)
     tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTCCTGCCCAGTAAGTCTCTGACAGAAAACGGCTAACACCCGTTTTATTTCCCCGCGCCCCCCGGAGTTTTCCAGGGCGGCTTTGTTTCTTTTTCATTCTTT‑‑CCcccgcccctccaa                                                                                                                                                                                                       >  1:849878/1‑233 (MQ=255)
          tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACCGTAAGTCTCTGAAAGAAAAACGCCAACACCCGATTTATTTCCCCACGCCCTCTGGGTTTTTCTCGGGCGGATTTGTTTTTTTTTTCTTCTTA‑‑CCCCCCCCCGTCAAAAAAtt                                                                                                                                                                                                  >  1:928065/1‑239 (MQ=255)
          tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGTGCCGCTGCACAATAAATATCTGACATAAACTGGCTAACCCCGTTGTTATTTACCGACGCCCCCTGGATGTTTCGTGGGGGGATTTGTTTTTTTGTCCTTCTTG‑‑Ccccccccctccaaaaaatt                                                                                                                                                                                                  >  1:249099/1‑228 (MQ=255)
          tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCATAAAACTCTGACAGAAATAGGCTAACACCCGGTTAATTTCCCGCCGCCCCCTGGGTTTTTCTAGGGCGGATTTGTTTTTTTGTCCTTCTTTG‑‑CCCCCCCCCCCaaaaaatt                                                                                                                                                                                                   >  1:998356/1‑236 (MQ=255)
          tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACAGTCAGTCTCTGTCAGAAATAGGCTAACACCCGGTTTATTTCCCGACGCCCCCTGTGGTTTTCTACGGCGGATTTTTTTTTATGTCCTTTTTT‑‑Ccccccccccccaaaaaatt                                                                                                                                                                                                  >  1:1250570/1‑228 (MQ=255)
          tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCCGGCCCACAGCCCCATAAATTTCTGACAGAAATACGCTAAAACCCGGTTTATTTCCCGGCTCCCCCTGTGCTTTCCCCGAGTGCATGTGTTTTTATGTCCCTCCTGTCCCCCCCCCCGCAAAAatt                                                                                                                                                                                                    >  1:1184080/1‑238 (MQ=255)
               gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCCTCACAATAAATCTCTCACCGAAAACGGGTCACACCCGGTTACTTTGCCCCCGCCCTCTCGAGTTTCCGAGGGCGTATTTGTTTTTATTTCCTTCTTTG‑‑CCCCCCCCCTCAAAAAAAtttc                                                                                                                                                                                                >  1:52323/1‑236 (MQ=255)
               gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCGTAAGTCTCTGACAGAAATCGGCTAAACACGGTTTAATTTTCCGCCGCCCCCTCGGTTTTCCGAAGGCGGATTTTTTTCTTTGTCCTTCTTG‑‑TCCCCGCCCCCTAAAAAAttttt                                                                                                                                                                                               >  1:211629/1‑236 (MQ=255)
                    gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTAATGCCCCATAAATTTCTGACCGAAAACGGCTAACACCGGCTTAATTTCCCGCAGCCCCCTGGGTTTTCTCAAGGGGGATTTTTTTTTTTTTTCTTTTTTT‑‑CCCCCCCCCCCACAAAAATTTTCATTTaa                                                                                                                                                                                        >  1:630820/1‑239 (MQ=255)
                               gTACTGGTCATATTGAAAACCGTTATAAGAACTGCTCTTACGACTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                                                                                                                                             <  1:1132223/239‑1 (MQ=255)
                               gTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCCCACAAAATCTCTGACAGAAATACGCTAACACCGGGTTTATTTTCCCCCGCCCCCTTGGGTTTTCTCGGGGGGGTTTGTTTTTTTTTTCTTCTTGTCCCCCCCCCCAAAAAAACATTTTCTTTTTaaactatcct                                                                                                                                                                                >  1:847671/1‑231 (MQ=255)
                                ttcTGGTCATTTTGAAAACCTTGATAAGAACTGCTTCTACGACTACCGGCTGCACCCGGCAATGTCTATTTCTAAAATCATTGGCTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                                                                                            <  2:997310/237‑1 (MQ=255)
                                  cTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaa                                                                                                                                                                          <  1:981383/239‑1 (MQ=255)
                                    ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTCCTGCACAGGAAATCTCTTACAGAAAACGGGTAACACCCGTTTTATTGCCCCGCCCCCTCCGGGTTTTTCCAGGGCGGGTTTGTTTCTTTGTCCTTTTTT‑‑TCCCCCCCCCTCAAAAAAATTTCACTTTTaatttaatttttttaat                                                                                                                                                                        >  1:1127628/1‑232 (MQ=255)
                                      gtattttGAAAACCGTGATAAGAACTGCCCTTACGGCTTCCTTCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                      <  2:52323/234‑1 (MQ=255)
                                       cATTTTGAAAACCGTGATAAGAACTTGTTTTACGACTTCCTGCTCCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                      <  2:998356/238‑1 (MQ=255)
                                       cATATTGAAACCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                      <  1:963601/238‑1 (MQ=255)
                                       cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCTGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                      <  1:843280/238‑1 (MQ=255)
                                        atatTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACAt                                                                                                                                                                    <  1:576871/239‑1 (MQ=255)
                                                  cctttaaaaGAACTGCTTTTACGACTACCGGCTGCCCCCGGCAATTTCTATTTTCAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                                                          <  2:669330/232‑1 (MQ=255)
                                                  cctgtaTAAAAATTGTTCTTCCGCTTTCCTGCTTCCCCCGGCAATTTATATTTCAAAATACATTGGGTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATACAATTAGATAACTACAGCCATTAGCca                                                                                                                                                          <  2:17297/234‑1 (MQ=255)
                                                  ccGTGATAAGAACTTCTTTTTCGACTACCTGCCGCACCCGGCAATTTTTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                                                          <  2:1250713/239‑1 (MQ=255)
                                                  ccGTGATAAAAACTGCCTTTACGGCTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                                                          <  2:1127628/239‑1 (MQ=255)
                                                            accTGCTCTTACGTCTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATc                                                                                                                                                <  2:249099/237‑1 (MQ=255)
                                                             aCTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGGCCTCCCGCCCAGAAAAGATCTGACAAAAAACAGCTAACACCGGGTTTATTTCCCCACGCCCCCTGGGTTTTTCTACGGGGGCTTTGTTTTTATTTTCTTTTTT‑‑GCCCCCCCCCCGAAAACATTTTTACCTTAAATTCAATTTATTTAAAACAACCATTAGAcaaaaaccgat                                                                                                                                                 >  2:1110921/1‑228 (MQ=255)
                                                                           tACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAGTCTCTTACAGAAAAAGGCTAACAACCGGTTTATTTCCCGGCCCCCCCCGGGTTTTTCCGGGGGGGATTTTGTTTTTTTTTCTTTTTTC‑‑CCCCCCCCCGACAAAAATTTTTCATTTTAATTTCATTTTGTAACTCACTCCCTTTACCCCACCGCCGTCCTTGTGCGAATtttt                                                                                                                                 >  1:814400/1‑239 (MQ=255)
                                                                                 ctgccaccGGCAATTTCTTTTTCTAAAATCCTTGGCGGTGGCGGTCCTTCTGCACAGTAAGTCGCTGGCAGAAATCGGCTAACGCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                                                                                                                                            <  2:193729/200‑1 (MQ=255)
                                                                                   tccccccGCATTTTTTATTTCTAAAATCATTGGGTGTTTCGGTCCTTCTGCACAGTAAGTTCTTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCGGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTTACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                                                                                                        <  2:498428/236‑1 (MQ=255)
                                                                                   gcACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                                                                                                        <  1:655245/239‑1 (MQ=255)
                                                                                     ccccGTCATTTTTTTTTTTTAAAATTATTGGCGGTTGCGGTTCTTCTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                                                                                      <  2:630820/238‑1 (MQ=255)
                                                                                        cGGCAATTTTTTTTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAAGCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTGG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                                                                                                                   <  2:849878/239‑1 (MQ=255)
                                                                                          gctatttttatttctAAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGGTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                 <  2:308220/236‑1 (MQ=255)
                                                                                          gCAATTTCTATTTCTAAAATCATTGGCGTTTGCGGTCTTACTGCACAGTAAGTCTCGGAACGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                 <  2:1269312/239‑1 (MQ=255)
                                                                                           cAATTTTTTTTTTTAAAACTAGTGGCGGTTGGGGTCCTTCTTCACACTAAGTCCGTTACAGAAATCGGCTAACTACGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCGCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                 <  2:928065/238‑1 (MQ=255)
                                                                                                ttttttttttaaaTCATTGGCGGTTGGGGTCCTACTGCCCAGGAAGGCTCGTACAGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                           <  2:1250570/229‑1 (MQ=255)
                                                                                                tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTA‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                           <  1:629925/239‑1 (MQ=255)
                                                                                                 ctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                                                          <  1:1110921/239‑1 (MQ=255)
                                                                                                        taAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcat                                                                                                   <  1:281442/239‑1 (MQ=255)
                                                                                                         aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                                                  <  1:192210/239‑1 (MQ=255)
                                                                                                           aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                <  1:912682/239‑1 (MQ=255)
                                                                                                           aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                <  1:316955/239‑1 (MQ=255)
                                                                                                                           ggTCCTACTGCACAGTAAGTCCCTGACAGAAATCGGCTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                                                                                      <  1:885265/201‑1 (MQ=255)
                                                                                                                                 cctctcacgTAAGTCTTTGACGAAATTGGGCTTACCTCGGTTTAATTTCCCCTCGTCTCCTGGTTTTGTCGAGGTCTTTTTTGTTTCTATTCCCTTCTTG‑‑CCCCCGTCCGTAAAAATATTTTCCCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGTGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg                                                                          <  2:313193/231‑1 (MQ=255)
                                                                                                                                 aCTGCCCAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg                                                                          <  1:774534/239‑1 (MQ=255)
                                                                                                                                 aCTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg                                                                          <  1:258600/239‑1 (MQ=255)
                                                                                                                                       caGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACCTTACATAACATCATATGTTGTTATAtt                                                                                      >  1:381701/1‑221 (MQ=255)
                                                                                                                                        cggtattctcTGACAGAAATCTGCTAACATCTGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTTCTTCTTG‑‑CCTCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatc                                                                                                     <  2:449937/199‑1 (MQ=255)
                                                                                                                                         gTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTCCATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcaaa                                                                                                  >  2:1326838/1‑205 (MQ=255)
                                                                                                                                         gTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                                                  <  1:1326838/207‑1 (MQ=255)
                                                                                                                                          tAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACCGCGCGATCCTGTGCGAAATTTTTTGATCTTCCTCCACATTACATAAAATCATATTTTGTTTTATTTATAATAcattttcatttt                                                                   >  2:1196185/1‑237 (MQ=255)
                                                                                                                                          tAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTTTCATGTTa                                                                  >  1:202955/1‑238 (MQ=255)
                                                                                                                                            aGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCt                                                               >  1:600504/1‑239 (MQ=255)
                                                                                                                                             gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                                                                                                                                     <  1:389599/153‑1 (MQ=255)
                                                                                                                                             gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                                                                                                                                     >  2:389599/1‑153 (MQ=255)
                                                                                                                                               ctctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCtttt                                                            >  2:211946/1‑239 (MQ=255)
                                                                                                                                                          aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTTTGCGAAATTTTGTGa                                                                                                                             >  2:71489/1‑164 (MQ=255)
                                                                                                                                                          aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa                                                                                                                             <  1:71489/164‑1 (MQ=255)
                                                                                                                                                            tCGGGTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATTTCCTTCTTG‑‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCaaa                                               <  1:890202/239‑1 (MQ=255)
                                                                                                                                                                 tAACCCTGGTTTAATTTCCCGTCCCCCTCTGGATTTTCCGAAGGTTTTTTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCCCTTTAAATTCAATTTTATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAActc                                            <  2:184481/238‑1 (MQ=255)
                                                                                                                                                                    cacCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTccc                                        <  1:1252955/239‑1 (MQ=255)
                                                                                                                                                                          gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGTTAACTACATCCATTAGCCACAGCGCGATCCTTTTCGAAATTTTACGATCTTCTTCCACATTACATAACATCATATGTTGTTATATCCATCATGCATTGTCAGGTTACCTTTTAAATACTTGCAAACTccccccacca                                   >  2:425850/1‑235 (MQ=255)
                                                                                                                                                                           tttAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACa                                   <  1:425850/238‑1 (MQ=255)
                                                                                                                                                                               aTTGCCCGACCCCCTCGGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTC‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATGTGATAACTACATCCATTAGCCACAGCTCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAGTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTa                              <  1:842461/239‑1 (MQ=255)
                                                                                                                                                                                    ccGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAtt                                                                           <  1:748896/189‑1 (MQ=255)
                                                                                                                                                                                    ccGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAtt                                                                           >  2:748896/1‑189 (MQ=255)
                                                                                                                                                                                     agacagccTTTGGATTTTCCGAGGGCTTTTTTTTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGAc                        <  2:270427/233‑1 (MQ=255)
                                                                                                                                                                                              ctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTg                           >  1:108200/1‑227 (MQ=255)
                                                                                                                                                                                                 gATTTTCCGAGGGCGTTTTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAAATCAATTTGATAACTGCATCCATTCGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTa            <  1:620455/239‑1 (MQ=255)
                                                                                                                                                                                                      tCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGtctgtct       >  1:270427/1‑233 (MQ=255)
                                                                                                                                                                                                              gctgtttttttTCTTTTTCCTTTTTT‑‑CCCCCCCCCGTAAAAATATTTTCTCTTTTAATTCAATTTGATAACTACATCCATTAGCCACAACCCGATCCTGTGCTAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATggg  <  2:125505/232‑1 (MQ=255)
                                                                                                                                                                                                                             tGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAt                                                                            >  2:1291660/1‑148 (MQ=255)
                                                                                                                                                                                                                             tGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAt                                                                            <  1:1291660/148‑1 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                
GTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGG  >  NC_000913/3499475‑3499914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: