Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,379:2 +C 6.1% intergenic (+585/+57) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3792.C 6.1% 193.3 / 2.9 65intergenic (+585/+57)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (29/32);  new base C (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTT  >  NC_000913/4296172‑4296595
                                                                                                                                                                                                                  |                                                                                                                                                                                                                            
aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTCTTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCAACCGACATCAAt                                                                                                                                                                                                        >  1:222606/1‑228 (MQ=2)
                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGGCAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGGGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcg                                                                                                                                                            >  1:390500/1‑228 (MQ=0)
                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                                                                                                                                            >  1:518909/1‑229 (MQ=11)
                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTCGGTCGGATCAGGCGTTTCCC‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGCCGCTGGCCCGGTCTTATCAGGCCACTCTTACCCGTTgg                                                                                                                                                       >  2:358848/1‑229 (MQ=11)
                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑ATGATTTTGTATGCCGGATAAGCCGTTT‑‑ACGC‑‑CGCATCCGACATCAATACCTGATGCGCCGCTTGCCGCTTCTTATCAGGCCTATCTTACCCGTTgg                                                                                                                                                       >  1:674098/1‑229 (MQ=11)
                                                   aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                                                                                                                    >  1:84299/1‑229 (MQ=19)
                                                          tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                                                                                                              >  1:609124/1‑228 (MQ=255)
                                                             tttAGCGTTATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGGTTAACGAGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                                                                                                                                           <  1:304443/228‑1 (MQ=255)
                                                              ttgactgttttttGTAGGGGGATTAAGGCGTTTGTGCCGACGCCGACATAAAGGCCGGGTGCGCCGCTGAGGGTGTTTTATCAGTCCGACGCCAGACAGCCCAATAGCCGGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                                                                                                                         <  2:532236/220‑1 (MQ=14)
                                                                    tGATTGTGTAGGTCGGATAAGGCGTTTATGCCGCATCCCACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                                                                                                                   <  1:252799/229‑1 (MQ=255)
                                                                        tttGGAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                               <  1:194917/229‑1 (MQ=255)
                                                                          tGTAGGGCGGATAAGGGGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg                                                                                                                             <  1:248102/229‑1 (MQ=255)
                                                                                  ggagtaGGCGTTTAAGGCCGATGCGACATCAACGCCTGGTGCGAAGGTTAACGGGTCTTATAAGGCCTACGCCAGACAGCGCAATAGCCTTATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                     <  1:299704/224‑1 (MQ=17)
                                                                                  ggATAAGTGGTTTAGGCCGCATCCGACATGAACGCCTGATGCGACGCTTAACTCGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                     <  1:701840/229‑1 (MQ=17)
                                                                                  ggATAAGGGGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGGTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                     <  1:573335/229‑1 (MQ=255)
                                                                                  ggATAAGGCTTTTAAGCCGCATCCGACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                     <  1:620986/229‑1 (MQ=255)
                                                                                  ggATAAGGCGTTTATGCCGCATCCGACATCAACGACTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                     <  1:644654/229‑1 (MQ=255)
                                                                                  ggATAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGCTGCGACGCTTAACGCGTCTTATCAGGGCTACGCAAGACAGCGCAATAGCCTGATTTAGC‑TGTATTTTGTAGGTCGGAGAAGGTGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                     <  1:358848/229‑1 (MQ=17)
                                                                                  ggATAAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                     <  1:41490/229‑1 (MQ=255)
                                                                                     tAAGGCTTTTTTGCCGCATCCGAACTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCACGTAAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc                                                                                                                  <  1:499751/229‑1 (MQ=17)
                                                                                        ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTGGGGTAATTTTTCAAACTTTTCACCCACGGTATCAAACCCTTc                                                                                                               >  2:410712/1‑229 (MQ=14)
                                                                                              tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCCATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTTTCAAACTCTTCCAACCCGGTATCaaa                                                                                                                     >  2:120035/1‑217 (MQ=14)
                                                                                              tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAACAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaag                                                                                                                     <  1:120035/217‑2 (MQ=255)
                                                                                                 tccgctacccgcATCAACGCCTGATGCGTCGCTTAACGCGTTTTAGCAGGACTAAGCCAGATAGCGCAATCGCCTGGTTTAGC‑GTGATTTTGTAGGGCGGATAAGGCGTTT‑‑ACGC‑‑CGCAGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                      <  2:205486/218‑1 (MQ=18)
                                                                                                  ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGTTCGGATAAGGCGTTT‑‑ACGC‑‑CGAATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTCATCAGGCCCATCTTAACCGTTGGTTAATTTTTCAACATCTTCACACCCGGTATCAAAACCTTCCATTCAACTc                                                                                                     >  2:307487/1‑229 (MQ=17)
                                                                                                   cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCTTACAGCTCa                                                                                                    >  1:157095/1‑229 (MQ=255)
                                                                                                    gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGCTAAGGCGTTT‑‑ACGC‑‑CGCATCCGACACAAATTCCTGATCCGACGATTGCCTCGATCGATCAGCCCTTTCTTAACCTTTGGTTACTTTTTCAAACACTTCACACCCGTAATCAAACCCTTCCATACacccccg                                                                                                   >  2:251989/1‑223 (MQ=14)
                                                                                                    gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCAACCCGGTATCAAACCCTTCAATACAGTTCAg                                                                                                   >  1:22226/1‑229 (MQ=255)
                                                                                                    gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTCCCCCCCCGGTATAAAAACCCTCcacacaaccccg                                                                                                   >  2:748365/1‑223 (MQ=14)
                                                                                                      aTCCTACAGCAACGCCTGATGAGACGCTGAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                 <  1:748881/229‑1 (MQ=18)
                                                                                                                   gccttacgcagcGCTTGATGTGTTTTTTCAGGTCTACGCCAGCCAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                    <  1:733733/218‑1 (MQ=21)
                                                                                                                   gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                    <  1:78321/229‑1 (MQ=255)
                                                                                                                      tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcgc                                                                                 >  1:594989/1‑229 (MQ=255)
                                                                                                                        aTGCGACGCTTAGCGCGTTGTATCAGTCCTACGCCAGACAGCGCAATAGCCGGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACTCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                               <  1:348928/229‑1 (MQ=21)
                                                                                                                             aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTg                                                                           >  1:426796/1‑228 (MQ=255)
                                                                                                                                        gTCTTATGAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTTGTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTTATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTtcttct                                                                <  2:609123/228‑1 (MQ=255)
                                                                                                                                          cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTtctc                                                               >  1:592274/1‑227 (MQ=255)
                                                                                                                                           ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTCTCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCgtt                                                            >  1:399749/1‑227 (MQ=255)
                                                                                                                                            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATa                                                           <  1:135650/229‑1 (MQ=255)
                                                                                                                                              ttcggCTTACGCCAGACAGCTAAATAGCCTGATTTAGC‑GTGATTTTGTAGGGCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa                                                         <  2:518909/226‑1 (MQ=25)
                                                                                                                                                    cTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGCTATCAAACCCTTCCATAAAGGTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttc                                                   >  1:269687/1‑229 (MQ=255)
                                                                                                                                                       cGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTTTCAAACCCTTCCCTCACGCCTAGGTTCAACCAGTGCACCGCCTTTTGCTTGTTTTTCTCGCTAAAACCttcttc                                                >  2:294451/1‑229 (MQ=21)
                                                                                                                                                            gACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTCGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGCCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCACACCCTTCCCTGCCGCTCTGGTTCAACCCGTGCACACCCTTTTGCCTGTTCTTCTCGATAAAACCTACTTCAcct                                            >  2:358949/1‑227 (MQ=21)
                                                                                                                                                               aGCGCCATAGCCTGATTTGGC‑GGTATTTTTTAGGTCGGATAAGGCGTTT‑‑A‑‑CGTCGCATCCTACATCAATGCCTGATGCTACTCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCGTCTTCACCGTTTa                                        <  2:157095/229‑1 (MQ=255)
                                                                                                                                                                 cgcCATAGCCGGATTTGGCGGTGTTTTTGT‑GGTCGGATAAGGCGTTT‑‑ACGC‑‑CCCATCCGACACCAACTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                      <  2:355016/229‑1 (MQ=37)
                                                                                                                                                                 cgcAATAGTCTGATTTGGC‑GTGATTTTTGAGGTCGGATAAGGCGTTTTCG‑‑C‑‑CGCAGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACGCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                      <  2:426796/229‑1 (MQ=37)
                                                                                                                                                                 cgcAATAGCCTGATTTAGC‑GTGATTTTGTATGTCGGATAATGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGTCTGATGAGACTCTTGCCGAGTCTTAGCAGCACTATCTTACACGCCTGTTAATTTTTCAACCCCGTCACCCCCGGTATCACACCATTCCATACCGCTACGGTTCACACAACGCCACGCCTTTCTCTAGTTCTTCTCCCTCAGACATTCTTCCCCGTTTaa                                       >  2:512632/1‑228 (MQ=9)
                                                                                                                                                                 cgcAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGTTCAGGt                                                                                                 >  1:229319/1‑170 (MQ=255)
                                                                                                                                                                 cgcAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                 <  2:229319/170‑1 (MQ=255)
                                                                                                                                                                 cgcAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                      <  1:431064/229‑1 (MQ=255)
                                                                                                                                                                 cgcAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGCTTaa                                       >  1:280310/1‑228 (MQ=255)
                                                                                                                                                                 cgcAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTAAGGTTCAACCAGGGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTaa                                       >  1:687773/1‑228 (MQ=255)
                                                                                                                                                                 cgcAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAATGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTaa                                       >  1:39973/1‑228 (MQ=255)
                                                                                                                                                                 cgcAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCGTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAACTTTTCAAACTCCTCACACCCGGTATCAAACCCTTCCATACAGCTTAGGTTCAACCAGACCAGCGCCCTTTCCCTGTTCTTCCACGTAAAACCTTCTTCACAGTTTaa                                       >  2:383582/1‑228 (MQ=21)
                                                                                                                                                                      ttgCCTGATTTAGC‑GTGATTTTGGAGGTCGGATAAGGCGTTT‑‑ACCC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCGTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcat                                 <  1:533392/227‑1 (MQ=255)
                                                                                                                                                                           tGATTTAGC‑GTGATTTTGTAGGGCGGGTAAGGCGTTTCCG‑‑C‑‑CGCATCCGACATCAATGCCTGATCCGACGCTTCCCGAGTCTTATCAGGCCTATCTTAACCGTTGGTTACTTTTTCAAACTCTTCACCCACGGTATCAAACCCTTCCATACAGCTCAGTTTCAGCAATCACAGGCCCATCTCCTTGGTCTTTTCGATAAAACCTTCCACACCGATTAAGAACACCAtt                             >  2:712622/1‑228 (MQ=255)
                                                                                                                                                                                tAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACCC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                       <  1:410712/229‑1 (MQ=255)
                                                                                                                                                                                   c‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCCGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAg                    >  2:95873/1‑229 (MQ=255)
                                                                                                                                                                                   c‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATATTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAACGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTCCTTAACCGTTTAAGAACATACTTCcccccccg                    >  2:3296/1‑227 (MQ=37)
                                                                                                                                                                                       ttttttttAGGTGGGGTATGGGGTTT‑‑T‑‑CCGCGCCTCCGACATCAATGCCTGATGCGAAGCTTGCCGCTTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACTCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc                 <  2:84299/227‑1 (MQ=255)
                                                                                                                                                                                            tgtgGGTCGGTTAAGGGGTTTCCG‑‑C‑‑CGCATCCGACCTCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGa            <  2:39973/229‑1 (MQ=255)
                                                                                                                                                                                                 ggcGGATAAGGCTTTT‑‑ACGC‑‑CGCATCCGACATCAATTCCTGATGCCACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc       <  1:272117/227‑1 (MQ=255)
                                                                                                                                                                                                 gTCGGATTAGGCGTTT‑‑ACCC‑‑CGCATCCGACAATAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc       <  1:336498/229‑1 (MQ=255)
                                                                                                                                                                                                   cGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCg     >  1:463050/1‑229 (MQ=255)
                                                                                                                                                                                                      aTAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt  <  1:466047/229‑1 (MQ=255)
                                                                                                                                                                                                                  |                                                                                                                                                                                                                            
ACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC‑GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTT  >  NC_000913/4296172‑4296595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑