Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,695,755	T→C	14.8%	E106G (GAG→GGG)	uidA ←	beta‑D‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,755	0	T	C	14.8%	133.3 / 3.0	62	E106G (GAG→GGG)	uidA	beta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base C (5/5);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGAT  >  NC_000913/1695539‑1695980                                                                                                                                                                                                                           |                                                                                                                                                                                                                                   gtgtAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGCCTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCt                                                                                                                                                                                                                        <  1:126412/229‑1 (MQ=255)      tagttttttgCTGTGGTGGATCCTGGCATAGTTAAAGAAATCATGGAATTAAGGCTGCTTTTTCTGGCCGTTTTCGTCGGTAATCACCATTCCCGGGGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGatta                                                                                                                                                                                                                   <  2:283073/220‑1 (MQ=255)            tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCCGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCACTAACATACGGCGTGCCATCGGCTTCCAATGCCGAATAGCCGCCCCGAGTCCCCCCCACTTCCTCTTTATTTAc                                                                                                                                                                                                              >  2:707860/1‑228 (MQ=255)            tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCCGTTCGTTGTTCACAA‑CAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACCGCCGGACATCGGATTCAAAAGGGGTTAAGCCGCCCCTCTGCCCCATCACTTCCCGCTTAATGAc                                                                                                                                                                                                              >  2:730647/1‑228 (MQ=255)            tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGTGGATACG‑GACACTTTTCCCGGCAATAACATAAGGAGTGACATCGGCTTCAAATGGCGGATAGCCGCCCTGATCCTCCATCACTTCCTGTTTATTGAc                                                                                                                                                                                                              >  1:413077/1‑228 (MQ=255)            tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGAc                                                                                                                                                                                                              >  1:160731/1‑228 (MQ=255)            tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTAATGGTCCATCAATTCCTGATTATTGAc                                                                                                                                                                                                              >  2:47200/1‑228 (MQ=255)             aCGCTGCGATGCATCCCGGACTAGTTAAAGAAATCCTGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGTCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGAc                                                                                                                                                                                                              <  1:730648/227‑1 (MQ=255)              cGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGATTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAAAGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCCGCTGTCCCATCCATTCCCGATTCTTGACCca                                                                                                                                                                                                           >  1:270153/1‑229 (MQ=255)                    gATGGATCCCGGGATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACt                                                                                                                                                                                                       <  1:411705/227‑1 (MQ=255)                    gATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGCCTGGTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGTTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGatta                                                                                                                                                                                                                   <  1:720471/215‑1 (MQ=255)                    gATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATATGCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCAGGACCTCGGCTTCAAATGGCTTATAGCCGCCCTGATGCCCCATAACTTCCTGCTTATGAAccccacccc                                                                                                                                                                                                      >  2:720470/1‑222 (MQ=255)                      ttgATCCCGGGATATGTAAAAGAATCATGGAAGGAAGTCTGTTTTGTCTGTGCGTGTTCTGCGGTAATCACCATTCCCGGGGGGATAGTCTGCCAGTTCCGTTCGTTGTTCACAC‑AAACGTGTATACG‑TACCCTTTTCCCGGCAATAACATACCGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCGCCATCACTTCCTGATTATTGACCCACACTTTGc                                                                                                                                                                                                   <  2:594930/227‑1 (MQ=255)                             cGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCCAATGGCGTATAACCGCCCTGATGCTCCATCACTTCCTGATTATTGAACCACCCTTTGACGTact                                                                                                                                                                                             >  1:632925/1‑226 (MQ=255)                                aataagtAAAGAATGCATGGAAGTAAATCTGGTTTGTCTTGCCGTTTTCCTCCGTAACCACCATTCCCGGCGGGATATTCTGCCCGTTCCGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACCCTTTTCCCGGCAATAACATTCGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGt                                                                                                                                                                                         <  2:632924/223‑1 (MQ=255)                                                      tttagccTGCTTTTTTTTGCCGTTTTCTTCGGTAATTCCCATTCCCGGTGTGATAGTCTGCCAGTTCAGTTCGTGGTTCACAC‑AAACGGTGATACG‑TACGCTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACg                                                                                                                                                                   <  2:349673/223‑1 (MQ=255)                                                                cttttttTGCCTGTTTTGTCGGTATACCCCATTCCCGGGCGGATAGTCTGCCCGTTCAGTTCGTTGTTCCCAC‑AAACGGTGCTACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCAGATTATTGACCCACACTTTGCCGTAATGAGTGTCCGCATCGAAACCCAGCACGATACGCTgg                                                                                                                                                          <  2:196837/227‑1 (MQ=255)                                                                         ccGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTTACCCACACATTTCCGTCATTAGTGACCGCAGCGAAACGCAGCAACAAACCCTGCCCCCCCCAAc                                                                                                                                                >  2:97182/1‑229 (MQ=255)                                                                             ttccgtcgGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCCCCC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACTTCGGGTTCAAATGGCGTATAGCCGCCCTGATTCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCttt                                                                                                                                            <  1:53836/226‑1 (MQ=255)                                                                              ttcttcGGTATTTCCCATTCCCGGGGGTATAGCTTGCCCTTCCAGTTCGTT‑TTCCCACAAAACGGTGATCCG‑TAACCTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTAAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  2:383836/229‑1 (MQ=255)                                                                              ttCGTCGGTAATCCCACTTCCCGGCGGGTTATCCTTCCCACTCAGTTCGTTTTTCACAC‑AAACGGTGATCCC‑TACCCGTTTCCCGCCAATAAGAGACGGCTTGACATCGGTTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  2:551787/229‑1 (MQ=255)                                                                              ttCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACATTTTTCCCGGCAATAACATCCGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTGCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  1:457624/229‑1 (MQ=255)                                                                              ttCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  1:47200/229‑1 (MQ=255)                                                                                           aCCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACtt                                                                                                                              <  1:673219/229‑1 (MQ=255)                                                                                                tCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCCCACTTTGCCGTAATGAATGACCGCATCGAAACGAGACACCTAACGCCGGCCTGCCCAACCTTTCGGTATaaaagacacacg                                                                                                                          >  2:73354/1‑219 (MQ=255)                                                                                                tCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCCTGCCTGCCCAACCTTTCGGAATAAAGACTTcgcg                                                                                                                          >  1:436595/1‑228 (MQ=255)                                                                                                tCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTcgcg                                                                                                                          <  1:97182/228‑1 (MQ=255)                                                                                                    ggcggGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGGATAAAGACTTCACGCTGa                                                                                                                      >  1:257726/1‑228 (MQ=255)                                                                                                     gcggGATAGTCTGCCAGGTCAGTTCGTTGTTCACAC‑AAACGGGGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCCCCATCACTTCCTGCTTATTGACCCACCCTTTGCCGTAATGAGTGACCGCCACGAAACGCAGCACGATACGCTGGCCTCCCCAACCTTGCGTTATAAAAACATCCCGCTgca                                                                                                                     >  2:56142/1‑226 (MQ=255)                                                                                                                  ccAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGAAACCAGCCGTTGccc                                                                                                       >  1:112656/1‑229 (MQ=255)                                                                                                                   cagttcagttCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGGATAAAGACTTCGCGCTGATACCAGACGTTGCCCg                                                                                                      >  1:176810/1‑229 (MQ=255)                                                                                                                   cagttcagttCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATAGCAACGACGCACGATACGCGTGCCCGGCCAACCTTTCGGTATAAAGCCATCGCGCAGATACCCGACGATccccc                                                                                                      >  2:479057/1‑228 (MQ=255)                                                                                                                             cgttcgtCACAC‑AAACGGTGATACG‑TACACTTGTACCGGCAATAACAGACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCCCACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCTGTATAAAGACTTCGCGCTGATACCAGACGTGGCCCGCATAATT‑ACg                                                                                           <  1:480152/223‑1 (MQ=255)                                                                                                                             ccgtgttCACAC‑AAAACGTTATCCGTTCCATTTGCCCCGACAAT‑ACCTGCGGCGTGACCTCGGCTTCAAATGGCGTAGAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACg                                                                                           <  1:36507/226‑1 (MQ=255)                                                                                                                             cGTTGTTCACAC‑AAACGGTGATACG‑TTCACTTTTCCCCGCAATAACATTCGGCGTGACAGCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACg                                                                                           <  1:110121/229‑1 (MQ=255)                                                                                                                             cGTTGTGCTCAC‑CAACGTGGATACG‑TACCAGTTTCCCGCAAATAACATACGGCGTGTCATCGGATTCAAATGGCGTATAGCCGCCCTGATGCCCCATCACTGCCTTATTATTGACCCACACTCTGCCGTACTGAGTGACTGCAACGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACg                                                                                           <  2:432125/229‑1 (MQ=255)                                                                                                                                                  aTACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAAAATCTGCATCGGCGAACt                                                                       >  1:222288/1‑229 (MQ=255)                                                                                                                                                   ttcG‑TACACTTTTCCCGGCAATAACATCCGGTCTGACATCGGTTTTAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTg                                                                      <  1:355312/227‑1 (MQ=255)                                                                                                                                                    ttgttgcacTTTTCCCTGCAATACCATGCGGCGTGACATCGGCTTCAAATGGCGTATGGCCGCCCGGATGCGCCATCACTTCCTGATTATTGACCCACACTTTGCCGTACTGACTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCTGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTg                                                                      <  2:270153/223‑1 (MQ=255)                                                                                                                                                    gcG‑TACACTTTTCCCGGCAATAACATTCGGCGTGAAATCGGCTTCAAATGGCGTATAGCCGGCCTGATGCCCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTg                                                                      <  1:345787/227‑1 (MQ=255)                                                                                                                                                       gttctcTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTGCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATc                                                                   <  1:517955/224‑1 (MQ=255)                                                                                                                                                           acTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTCTAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACTATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGACCGCATAATT‑CCGCATATCTGCATCGGCGCACTGACCGTTa                                                               >  1:687408/1‑229 (MQ=255)                                                                                                                                                               ttCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTTACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGAGACCAGACGGTGCCCGCATAATT‑ACGAATATCTGCATCCGCGACCTGAACGTTAAAAc                                                           >  2:735457/1‑229 (MQ=255)                                                                                                                                                               ttCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTGGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGCATATCTTAATCGGCGTACTGACCGTTaaaa                                                            >  1:440028/1‑228 (MQ=255)                                                                                                                                                                  ccGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCAAACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGAATAAAACATTCCCGCTGATACCAGACGTTGCCCGCATAATT‑ACGCAAATCTGCATCGCCGCAATGAACGTTAAAAcgac                                                        >  2:4202/1‑226 (MQ=255)                                                                                                                                                                         tAACATACGGCGTTACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGcac                                                 <  1:707861/229‑1 (MQ=255)                                                                                                                                                                                   ggTGACCTCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGcc                                       <  2:590565/228‑1 (MQ=255)                                                                                                                                                                                   cGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCCTCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCCTAATT‑ACGAACAACTGCCTCGGCGAACCGAACCTTAAAAAGGCCAGGCACACCAAATGcc                                       >  2:352443/1‑229 (MQ=255)                                                                                                                                                                                   cGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           >  1:227298/1‑130 (MQ=255)                                                                                                                                                                                   cGTGACATCGGCATCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  2:227298/130‑1 (MQ=255)                                                                                                                                                                                     tGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGCCGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGACCTGCTCTGTAAACCTGCCTGGCACAGCAATTGCCCg                                     >  1:484763/1‑229 (MQ=255)                                                                                                                                                                                        cATCGGCTTCAAATGGCGTATAGCCCCCCTGATGCCCCATCCCTTCCTGGTTATTGACCCACCCTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCt                                  <  2:160731/229‑1 (MQ=255)                                                                                                                                                                                             gCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCg                                                                  <  1:186853/192‑1 (MQ=255)                                                                                                                                                                                             gCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATATGCATCGGCGAACTGATCg                                                                  >  2:186853/1‑192 (MQ=255)                                                                                                                                                                                             gCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGAGCTGATACCAGACCTTTCCCGCCTAATT‑ACGAATATATGCATCGGCGAACTGATCGTTAAAACCCCCTGGCAAAACAATTGCCCGGCcttctt                             >  2:568244/1‑229 (MQ=255)                                                                                                                                                                                                      tgcttttaGCCGCCCTTGTTCCCCATCCCCTCCGGATTATTGACCCACACTTTGCCGGAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACgcgc                    <  2:257726/223‑1 (MQ=255)                                                                                                                                                                                                         ctattGCCGCCCTTATGCCCCCTCCCTTCCTGATTATTGACCCCCCCTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATTCGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCttt                 <  2:176810/228‑1 (MQ=255)                                                                                                                                                                                                          tataGCCGCCCTGGTGCACCAGCACTTCCTGATTTTTGACCCACACTTTGCCGTAATGAGTGACCGCATCAAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCttt                 <  1:739294/228‑1 (MQ=255)                                                                                                                                                                                                                cgccCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGACCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGGGCTTTCccccca          >  1:426325/1‑229 (MQ=255)                                                                                                                                                                                                                  cccTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGACACGCAGCACGATACGCTGGCCTGCCCAACCTTTCCGGATAAAGACTTCGCGCTGATACCAGACGCTCTCCGCATAATTCACAAAT‑TCTGCATCGTCGAACTGCCCGTTAAACCTGCCTGGCCCACCAATTGCCCTGCTTTCTTGTAACCCGCCTTCcccccacc        >  2:91614/1‑227 (MQ=255)                                                                                                                                                                                                                       aggCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGGATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATCATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAACCTGCCTTGCCCAGAAATTGCCCGGGTTTCGTGTAAAGCCCTTTCCCACAAACGCTgc   >  2:721473/3‑228 (MQ=255)                                                                                                                                                                                                                          cTCCATCCCTTCCTGATTATTTACCCACACTTTTCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCCGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGAt  <  1:73354/227‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                   GTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGAT  >  NC_000913/1695539‑1695980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑