Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 715,338 | T→A | 15.5% | intergenic (+140/‑74) | pgm → / → ybfP | phosphoglucomutase/lipoprotein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 715,338 | 0 | T | A | 15.5% | 154.3 / 4.1 | 63 | intergenic (+140/‑74) | pgm/ybfP | phosphoglucomutase/lipoprotein |
Reads supporting (aligned to +/- strand): ref base T (40/12); new base A (2/9); total (42/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.36e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTG > NC_000913/715128‑715562 | gttaagaaCATCGGAAGCAGATTGAAAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACCCA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCAt < 1:96546/226‑1 (MQ=255) tGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAAGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGACTGATGCGTTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGGATTTAAGCGATTTTGTAAGC‑CGGATAAGGCGTTAACGCCGCATc > 1:448155/1‑229 (MQ=255) aaCATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAACGATTTTGTAGCC‑CGGATAAGGCGTCCACGCAGCATCCGGcc > 1:432867/1‑228 (MQ=255) aTCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTGAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCaaaa < 1:571026/229‑1 (MQ=255) cGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAaac > 1:651338/1‑229 (MQ=255) cAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCGGATGGTGCAATATATTGACTTTAAGCGTTTTTGTAGGC‑CGGATAAGGCGTTCACGCGCCATCCGGCCAAaacaa > 1:473433/1‑229 (MQ=255) aTTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGGTAAGGCGTTCACGCCGAATCCGGCAAAAAAAACGAaaac > 2:64285/1‑229 (MQ=255) aagCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGCTTTTGTAGGC‑CGGTAAAGGCGTTCAGGCCGCATCCGGCAAAAAAAACGAAAAATTTTAcaac > 2:286820/1‑228 (MQ=38) agCTGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑ATTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑AGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTcaacaa < 1:375314/229‑1 (MQ=255) agggTTTAGATTTGTTAGCGAAGTTTGTAAAACCGGGTAAACTCA‑TTTAAAAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGAAAAACACAGAATTGCCTGATGGGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGGTAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTcaacaa < 2:52135/227‑1 (MQ=38) gaga‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGAAAAGGCGTTCACGCCGCATCCGGCAAAAGCAACGAACACTTTGTCAACAAAccaaga > 2:445466/1‑224 (MQ=255) ttAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAACTCaa > 1:256011/1‑229 (MQ=255) ccGAATTTCTCAAACACGCTTAAA‑ACATTTTAAAAAAAAAAGGGCCGGCGTAAGATCCACATTTTTTACTTAGGACAAACACAGAAATGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGTAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGa < 2:568844/228‑1 (MQ=21) gAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACTCTTAGCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATg < 1:624072/229‑1 (MQ=255) cgcgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCTGCAAAAACACCGAACACTTTGTACAAAAACTGAGTAGATCAACGAAAACCAAATaaaaaac > 2:214086/1‑225 (MQ=37) cgcgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGAAGCTCAAGGAAATCCCAATGAAGa > 1:346693/1‑227 (MQ=255) cgcgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAATAGCCCAAGGAAAACCCAATGAAAAca > 2:280876/1‑228 (MQ=255) cgcgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTAAGTAGCCCAAGGAAATCCCAAAGAAAAcc > 2:422247/1‑229 (MQ=255) cgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATAAAGACCaa > 1:499886/1‑229 (MQ=255) ttaaCACA‑TTGAAAAAAAAAAGGGCTGTTCCAAGATCGCTCTTTTTTCCGGAGTCCAAACATATAATTGCTTGATTGGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGTCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACa < 1:744314/227‑1 (MQ=37) ttAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTCCGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGCTAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCACAAAACTGCGGAGCTCAAGGAAAACCAAAGAAAGACCAACAagagaagtg > 2:756026/1‑224 (MQ=37) aaaaaaGGGCGGTCGCAAGGTCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCa < 1:411911/229‑1 (MQ=255) aaaaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGAAAAAACAACGAACACTTTGTCACCAAACTGAGTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGACTGGCCGTCCTa > 1:334582/1‑229 (MQ=255) gCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGt > 1:625598/1‑211 (MQ=255) gCGGTCGCAAGATCGCCCTTTTTTACGGATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGt < 2:625597/211‑1 (MQ=255) cgttCGCAAGAGCGCCTTTTTTTACGTATGACAAACACAGATTTGGCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGAAAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCAc < 2:334582/226‑1 (MQ=255) ggTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGCCAAAAGGTCACt > 2:340060/1‑229 (MQ=255) gTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAg > 1:8900/1‑196 (MQ=255) tCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATAAAGACCAACAGGAGTCTGGGCGTCATAGTGTCACTaa > 1:311813/1‑229 (MQ=255) tCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCAATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCACCAAACTGAGAAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATATGGTCACTac > 1:338225/1‑228 (MQ=255) tccgctttttttACATTTGACAAACAAAGAATTGCTGGATGCGCTGCGCTTATGAGGCATACGAGGAGGGGGAAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGAAAAAGCAACGAACACTTTGCCAACAAACTGAGTAGCCCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGc < 1:445466/222‑1 (MQ=37) ttttACGTATGACAAACACAGAATTGCATGATGCGCTACGCTTATCAGGCCTACGAGGTTGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGCTAAGGCGTTCAGGCCGCATCCGGCAAAAACAACGAACACTTTGTCACAAAACTGAGTCGCTCAAGGAAATCCCAATGACAACCACCAGGAGTATGGTCGTCATAGTGCAAGTACTTACCGcaccatgat > 1:315891/1‑220 (MQ=25) gACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCACCAGGAGTCTGGTCGTCATAGTGTAACAAATTACCGCCACATTACTGCTGACttc > 1:205877/1‑226 (MQ=255) cTGATGGGCTACGTTTACCGGGCCTAAGGGGATGGTGCAATTAATGGAATTAAAGCTATTTTGAAGGC‑CGGAAAAGGCGTTCACGCCGCATCCGGAAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACa < 1:64285/229‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGACGTCATAGTGTCACTAATTACCGCAACATTACTGCTGACTGCATGCGCCCAACCTGAACa > 1:543375/1‑226 (MQ=255) tGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGGCTGGTCGTCATACTGTCACTAATTACCGCCCCATTACTGCTGACTGCATGCGCCCAACCTGAACAatc > 1:116748/1‑228 (MQ=255) ccTACGTTTAACAGGCCTACGGGGATGGTGCAATATATGAAATTTAAGCGATTTTGTAGGC‑CGTAAAAGGCTTTCACGCCGCATCCGGAAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAAtcatca < 1:422247/228‑1 (MQ=255) aTCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGCCTGCATGCGCCCAACCTGAAAAATCACCATTAGCTg > 2:481984/1‑227 (MQ=255) ggCCTACGGGGGGGGCGAAATATATTAATTTAAAGCGATTTGGTAGCC‑CGGAAAAGGTGTACACGCCGCATCCGGAAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACt < 1:340060/229‑1 (MQ=255) gCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACt > 1:133682/1‑228 (MQ=255) gCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGACTGGTCGTCATAGTGTCACTAATTACCGCCACATAACTTCTGACTGCATCCGCCCAACCTGAACAATCATCATAAACTGGCGAcg > 2:655613/1‑227 (MQ=255) tACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGtt > 1:594494/1‑229 (MQ=255) cGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTAc > 1:459613/1‑229 (MQ=255) cGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTACACAACCATCATTAGCTGGCGACTGGTTAc > 1:407763/1‑229 (MQ=255) ggagagggtaaaaTATATTAAATTAAATAAATTTTGTAGGC‑CGAAAAGGGCCTCCACCACGCATCGGGAAAAAAAAACGAACACTTGAGCAACAAACTGAGTAGCTCAAGGAAACCCCAAGGAACACCAACAGGAGTCTGGTCGTCATAGTGTATCTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACt < 2:499886/218‑1 (MQ=255) gTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGGTCACGCCGCATCCGACAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATACCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCAACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTACCTGGCGACTGGTTACTGAcccc > 1:629757/1‑228 (MQ=255) tacAAGATATTGAATTTAAGC‑AATTTGGAGGCGCGAAAAAGGTTTGCACGCAGCAGCGGGAAAAAACAACAAACCTTTTGCAAACAAACGGAGGAGCTAAAGGAAAGCCCAATGAAACCCAACGGGAGTCGGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTa < 1:214086/227‑1 (MQ=255) gaaaataatTGAATTTAGGCGATTGTAAAGCC‑CGAAAAAGGCGTTCCCGCCGCATCCGGCAAAAACAACGACCACTTTGTAAACAAGCTGAGTAGCTCAAGGAAATCCCAATGAAGCCCACCAGGGGTCTGGTCGTCATGTTTTCCCTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTa < 1:535208/221‑1 (MQ=255) atatatTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAgc > 1:371009/1‑228 (MQ=255) atatTTAATTTAAGCGATTTTGTAGGC‑CGAATAAGGCGTTAACTCCGCAACCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGAAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTGGGCATCGTGTCACTAATTACCGCCACATTCCAGCTGGCTCCAGGCGCCCAACCTGAACTAGCATCAGTAGCTAGCGCCCGGTAACTGACCCCAAAAGaaa > 2:690943/1‑227 (MQ=255) tGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCACAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGCCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTCCTGACTTAATGCGCCCAACCTCAACAATCAGCATTAGCTGGCTACTGGTTAATGACCCCTAAAGTTAAAAc > 1:515147/1‑229 (MQ=39) tGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAg < 1:8448/83‑1 (MQ=255) tGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAg > 2:8448/1‑83 (MQ=255) tGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCTACTGTTTACTAACCCCTAATGATAAAAc > 1:672384/1‑229 (MQ=255) ttAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAACCCagag > 1:482828/1‑229 (MQ=255) aaGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAGCa > 1:430393/1‑177 (MQ=255) aaGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGAGTCACTAATTACAGCCACATTACTGCTGACTGCATGCGCCCAACCTGACCAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAACCCACAGGa > 1:1628/1‑229 (MQ=255) aaGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTATGGTCGTCATAGTGTCACTAATTACCGCCACATTACTCCTGACTGCATGCGCCCAACCTGAACAATCCTCATTAGCTGGGGACTGGTTATTGACCCCTAAAGATaaaaacctgtc > 2:535208/1‑221 (MQ=255) gtgttttGGGGGC‑CGGAAAGGGCGTCCCGCCCGCAGCCGGCAAAAACAACAAACCGTTGTTCAACAAACTGTGTAGCCCAAGAAACCCCCAAGGAAGACCAGCGGGTGGCTGGTCGTGATAGTGTCACTAATTTCCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTa < 2:515147/226‑1 (MQ=255) ctaTTTTATAGGC‑GGAATAAGGGTTTCCGCCCCCATCGGGAAAAAAAAACGAACCCTTTGCCAAAAAACTGGTTAGCCAAAGAAACCCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTTTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTa < 2:594493/227‑1 (MQ=255) c‑cGGAAAAGGGTTTCCCGCCGCATCCGGAAAAAACAACGAACACTTTGTCAACAAACTGATTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGTTCGTCATAGTGTCATTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCAtt < 1:280876/229‑1 (MQ=255) cGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCag > 1:551446/1‑209 (MQ=255) cGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATACCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTCCTGACTGCCTGCGCCCAACCTGAACAATCATCATTAGCTGGCGCCCGGTTCCTGACCCCTAAACATAAAACAAGAGGATTAAAgggaaaagttg > 2:648586/1‑221 (MQ=255) | GTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTG > NC_000913/715128‑715562 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |