breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorspgi-1-1_S1_L001_R1_001.good.fq757,102166,378,528100.0%219.8 bases229 bases96.5%
errorspgi-1-1_S1_L001_R2_001.good.fq757,101167,215,096100.0%220.9 bases229 bases72.8%
total1,514,203333,593,624100.0%220.3 bases229 bases84.7%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65262.85.7100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000002387
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500080
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.008

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.91265

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input13:14:41 29 Feb 202013:15:03 29 Feb 202022 seconds
Read alignment to reference genome13:15:03 29 Feb 202013:23:35 29 Feb 20208 minutes 32 seconds
Preprocessing alignments for candidate junction identification13:23:35 29 Feb 202013:23:58 29 Feb 202023 seconds
Preliminary analysis of coverage distribution13:23:58 29 Feb 202013:24:59 29 Feb 20201 minute 1 second
Identifying junction candidates13:24:59 29 Feb 202013:25:00 29 Feb 20201 second
Re-alignment to junction candidates13:25:00 29 Feb 202013:26:31 29 Feb 20201 minute 31 seconds
Resolving best read alignments13:26:31 29 Feb 202013:27:10 29 Feb 202039 seconds
Creating BAM files13:27:10 29 Feb 202013:28:03 29 Feb 202053 seconds
Tabulating error counts13:28:03 29 Feb 202013:28:25 29 Feb 202022 seconds
Re-calibrating base error rates13:28:25 29 Feb 202013:28:26 29 Feb 20201 second
Examining read alignment evidence13:28:26 29 Feb 202013:59:50 29 Feb 202031 minutes 24 seconds
Polymorphism statistics13:59:50 29 Feb 202013:59:50 29 Feb 20200 seconds
Output13:59:50 29 Feb 202014:00:08 29 Feb 202018 seconds
Total 45 minutes 27 seconds