Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,466,729 | G→A | 13.4% | pseudogene (1338/2513 nt) | ydbA → | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,466,729 | 0 | G | A | 13.4% | 90.9 / 3.6 | 35 | pseudogene (1338/2513 nt) | ydbA | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
Reads supporting (aligned to +/- strand): ref base G (21/8); new base A (2/3); total (24/11) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.00e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
GCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCAC > NC_000913/1466467‑1466992 | gcggcggTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATACAGGTTGACGGAGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TACACCGGTACGGAAATTGCCGGCAAAAACGGGAAGGTTATTCAGGAACGCGACCTGGaggc > 2:312983/1‑286 (MQ=17) cACGATATTGATATCACCGGCGACAGCGCAACTGTGGATAACAAGGGCACCATGCCCGTCACCGCTCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGTGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCTTTGCCGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcg < 1:312983/289‑1 (MQ=255) tttGAGATCACCGGCGAAAGCGAAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTAGCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGAACCGGCACGCAGATTAACGGGGATGACGCCACGGAAAACAACAAGGGCAAAAGCAGCGTCGGAGGTAAGGAA‑GACACCGGTACGGAAATTACTGGCAATAACGGAAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑Gt < 2:73482/287‑1 (MQ=17) gCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAg < 2:409884/265‑1 (MQ=255) gCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCAATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAg > 1:409884/1‑265 (MQ=255) cGGTGGAGAACAAGGGCACCAGGACCGTAACCGATCCGGAGTCCATCGGTATCAAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCa < 2:138542/265‑1 (MQ=255) cAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGat > 1:138542/1‑265 (MQ=255) gggCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCTATCACCAATGGTGGCACCGGCACGCAGATTAACGGTTACGACGCCACGGCGAACAACAACGGAAAAACCACCGTTGACGGAAAGGAT‑TACACCGGTACGGAAATCGCGGGCACTAACGGGAATGTGATTCTGGATGGCGACCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATAACATCGGCGACAGCGCAACGGTTGac > 2:228555/1‑288 (MQ=17) tCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTCAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGACGACGCCACCGCAAATAACAGCGGCAAAACCACCGTTGACGGCAAAGAT‑TCCACGGGTACCGAAATTGCTGGCAATAACGGGAAGGTAAATCAGGACGCCTATATACATGTAAGC‑GTGGCGAGGCACGGTATTGTTAAACCCGGCGAGAGCGCCGTGGTGTATAACAAaggaatcaa > 2:336426/1‑283 (MQ=255) gAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCAGCCTGACCGTCAcc > 1:364916/1‑288 (MQ=255) cATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGACGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCCACGGTGGATAAAAAGGGCACCATGACCGTCACAGATCCg > 1:132227/1‑289 (MQ=255) aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTAATTCAGGACGGGGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAAAGGTGGATAACCACGGAACGATGACCGTCACCGATCCgg > 2:411166/1‑289 (MQ=255) tCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATCGCTGGCAATAACGGGAAGGTGATTCAGGACGGAGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACCGCGCAACGGTGGCTAACAAGGACATCATGACCGTCACAGAACCGGAGTcc > 1:219691/1‑287 (MQ=255) gACGGCTACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCa < 2:393450/206‑1 (MQ=255) gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGCTCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATGACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTAccc > 1:213560/1‑289 (MQ=255) gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGACGACGCCACGGCAAATAACAGCGGCAAAACCACTGTTGACGGAAAGGAT‑TCCACCGGTACCGAAATCGCTGGCAATAACGGGAAGGTGAATGTGGACGGCGACCTGAATGTTACCGGCGGTG‑AGCATGGTATTGAGAGCATTGGTGACAGCGGAACGGTGGACAACAAAGGAACTATGGCTGTTACCGATCCGCAgcctattggcttgc > 2:202533/1‑276 (MQ=255) gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAACGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCa > 1:393450/1‑206 (MQ=255) cGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGt > 1:357452/1‑208 (MQ=255) cGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGt < 2:357452/208‑1 (MQ=255) cGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGAAAATTGCGGGCAATAACGGGAGGGTGATTCAGGACGGCGACCTGGATGTCAGCGGCGGCG‑CGCACGGTATTGAGAACACCGGCGACAGCGCCACGGTGGATAACAAGGGCACCATTACCTGCACCGATCCGGAGTCCATTGGGATcccg > 2:129363/1‑287 (MQ=17) cGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAg > 1:271351/1‑223 (MQ=255) cGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAg < 2:271351/223‑1 (MQ=255) cgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTAAATAACGAAGGc > 2:356491/1‑289 (MQ=255) aCGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGCTTGACGGCGACGAGGCCATCCTCAATACACAAGGCGCGCGCACTa > 1:387768/1‑288 (MQ=255) atgaCGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGCTCa > 1:322768/1‑288 (MQ=255) atgaCGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCa > 2:27951/1‑288 (MQ=255) atgaCGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGAGTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCa > 1:91957/1‑288 (MQ=255) agacaacGGAAAAACCACCGTGTACGGC‑AGTATGTCCACCGGTACGGAAATTGCGTGCAATAACGGGAAGGTGAGTCAGGACGGCGATCTGCATTTCTGCGGCGTCG‑GTCGCGGTATTGCGAGCACCGGCAAAAGCGCAACGGCGGCAACCTAGGGTACCTTTAAGGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCGATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCAcc < 2:132227/287‑1 (MQ=255) cGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGCTTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACCCAGATCAACGGTAACGACGCCCCCGCGAATAAAAGTGGaaaa > 2:61322/1‑289 (MQ=255) tatccgCAAGGAT‑TCAACCGGTACGGAAATAAACGGTAATAACGGAAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTGACGGTATTGATATCACCGGGGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAAc < 2:159854/283‑1 (MQ=14) ggAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGTTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCa < 1:298417/257‑1 (MQ=255) ggAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCa > 2:298417/1‑257 (MQ=255) t‑tCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGcggcac < 2:165953/225‑1 (MQ=11) t‑tCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGcggcac > 1:165953/1‑225 (MQ=11) cggaaatcaacggtaaTAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCAc > 2:180109/15‑289 (MQ=11) | GCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAT‑TCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCG‑GTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCAC > NC_000913/1466467‑1466992 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |