Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,126,3400CT35.7% 42.4 / 22.1 44T157T (ACG→ACA) priAPrimosome factor n' (replication factor Y)
Reads supporting (aligned to +/- strand):  ref base C (15/12);  new base T (12/3);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATCGGCGCGTTCGCC  >  NC_000913/4126205‑4126481
                                                                                                                                       |                                                                                                                                             
ttCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGAAAAGCGGCATCATTAAATTCGAGTGTGGCgc                                                                                                                                        >  1:453692/1‑142 (MQ=255)
ttCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGAGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGCGCGTGGCGa                                                                                                                                        >  2:579599/1‑143 (MQ=255)
       ttCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGTGTGGCGACCTGGTc                                                                                                                                 >  1:599857/1‑143 (MQ=255)
       ttCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGTGTGGCGACCTGGTc                                                                                                                                 >  2:507113/1‑143 (MQ=255)
           agcGCAACCGCTCACCAGAAACGGCATAGTGCGTTCGCCAGTCGCTAAACTCTGGTGTGTCACTTGCCAAGTCACACAGACCGTTTTTGCGTAGCGCCTGCACCGCGGCATCATTAAATTCGAGAGAAGCGACCTGGtagaac                                                                                                                             >  1:120686/3‑138 (MQ=255)
            tCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGCCCTTTTTTGCGTAGCCCCTGCGTCGAGGCATCCTTAAATTCGCGTGTGGCGCCCCGATCGCGcc                                                                                                                            >  2:107583/1‑143 (MQ=255)
             cGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAAACACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCa                                                                                                                           >  1:458390/1‑143 (MQ=255)
                    gCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTATCTCCTGCAACGCAGCATCGTTAAATTCGAGTGTGGCGACCTAGTCGCGCCAGATTttg                                                                                                                    >  2:407824/1‑143 (MQ=255)
                         ccAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTttgccttg                                                                                                               <  1:576508/143‑1 (MQ=255)
                          cAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGTCTGCGCCAAACTatgccttcc                                                                                                              >  2:41572/1‑141 (MQ=255)
                          cAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGCTGTTTCACTTGCTAAGTCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCACTATTAAAGTCGAGTGTGGCGACCGGGGCGCGCCAGCTGttgccttgc                                                                                                              >  2:655952/1‑143 (MQ=255)
                                   cATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAAcgcc                                                                                                     <  1:669783/143‑1 (MQ=255)
                                     tAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAAcgccgc                                                                                                   <  1:290648/143‑1 (MQ=255)
                                            ttcgCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGcc                                                                                            >  2:542662/1‑143 (MQ=255)
                                                ccAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGAGGCATCATTGAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCtgtt                                                                                        >  1:570810/1‑143 (MQ=255)
                                                       cTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCta                                                                                 >  1:538426/1‑142 (MQ=255)
                                                             tctGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTCGCGCCCCCAGCGCCTTTTGTTGCTTgggggg                                                                           >  1:227321/1‑142 (MQ=255)
                                                               tGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGACCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGc                                                                         >  2:10419/1‑143 (MQ=255)
                                                               tGGTGTTTCACTCGCTAAATCACACAGACCTTGTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGTGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGc                                                                         <  1:521823/143‑1 (MQ=255)
                                                               gtgtgtTTCACTTGCTAAATCGCACAGACCTTTTTTGCGTAGCGGCTGTAGCGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGc                                                                         <  1:658449/141‑1 (MQ=255)
                                                                        aCTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGTGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGc                                                                >  1:593867/1‑143 (MQ=255)
                                                                          ttGCTCAATCACACAGCCCTTCTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGTGTGGCGACCTGGTCGCGCCATATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTg                                                              <  1:29589/143‑1 (MQ=255)
                                                                            gCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGTGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGATGTTGCTTGGGGGGGCGTTGCAGGCTGtt                                                            >  1:7980/1‑143 (MQ=255)
                                                                            gCTAAATCACACAGACCTTTGTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGtt                                                            <  1:681740/143‑1 (MQ=255)
                                                                            ccTAACTCACACCGACCTTCTTTGCGTAGCGCCTGCAACGCGGCATCATTAGAGTCGAGCGTGGCGACCGGGGCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGtt                                                            <  1:84250/142‑1 (MQ=255)
                                                                             gtAAATCACACAGACCTTTTTGGCGTAGCCCCTGCAAGGCGGCATCATTAAATTCGAGTGTGGCGACCTGGTCGCGCCAGGTTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTc                                                           <  2:348580/142‑1 (MQ=255)
                                                                             cTAAATCCCACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTc                                                           <  1:188530/143‑1 (MQ=255)
                                                                              tAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTGGTTGCTTGGGGGAGGGGTGCAGGCTGTTCa                                                          >  1:52498/1‑143 (MQ=255)
                                                                                aaTCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGa                                                        >  1:94880/1‑143 (MQ=255)
                                                                                aaTCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCAAGATTTCAACTTGCCGTAACGCCGCCAGCGCCCGCTGTTGCTTGGGGGAGCGTTTCAGGCCGTTccgg                                                        >  2:633283/1‑140 (MQ=255)
                                                                                  tCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACATGGTCGCGCCAGATTTTGCCTTGCCGTCACGCCGCCTGCGCCTGTTGTTGCTTGGGGGGGCGTTTCAGGCTGTTCAGATc                                                      >  1:199467/1‑143 (MQ=255)
                                                                                            cTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCGGTTCAGATCCACCGCCTgg                                            <  1:320432/143‑1 (MQ=255)
                                                                                             ttttttGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGCTTCAGGCTGTTCAGATCCTCCGcactgc                                           >  2:109525/1‑138 (MQ=255)
                                                                                               ttttGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCATGGCCt                                         >  1:438951/1‑143 (MQ=255)
                                                                                                     gTAGCGCCTGCAACGCGGCATCATTAAATTCGAGTGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGCGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCa                                   >  1:625353/1‑143 (MQ=255)
                                                                                                          gcCTCCAACGCGGCATCATTAAGTTCGCGCGTGGCGACCTGGTCGCGGCAGATTTTGCCGTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGc                              <  2:252284/143‑1 (MQ=255)
                                                                                                                   gcgGCATCATTAACTTCGAGTGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTACCGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCCGGCCTTGTTCAGTGGCAACCCAGTa                     >  1:258347/1‑143 (MQ=255)
                                                                                                                   gcgGCATCATTAAATTCGAGTGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGCTCAGTGGCATACCCGTa                     >  1:119295/1‑143 (MQ=255)
                                                                                                                     ggCATCATTACATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTAcc                   <  1:215865/143‑1 (MQ=255)
                                                                                                                     ggCATCATTAAATTCGAGTGTGGGGAAATAGTGGCGCCAGATTTTGCCTTGCCGTAACGCCGGCAGCGCCAGTTGTTGCATGGGGGAGCGTTTGAGGCTGTTCAGATCCACCGCCTGGCCCTGTTCAGTGGCAAACCAGTAcc                   >  2:260627/1‑143 (MQ=255)
                                                                                                                     cgCATCCGTAAATTCGAGGGTGGCGACCTGGGCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTAcc                   <  2:145179/142‑1 (MQ=255)
                                                                                                                             tAAATTCGAGCGTGGCGACCTGGTCGCGCAAGATTACGACATGCTGTAACGCCGCCAGCGCCTGTTGCTGCTTGGGGGAGCGTTGCAGGCTGTTCAGATCCACCGCCTGGGCTTGTTCAGTGGCACACCAGCACGACATCgtc           >  2:258593/1‑141 (MQ=255)
                                                                                                                               aaTGCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATCGgcgc         <  1:10419/143‑1 (MQ=255)
                                                                                                                                      gCGTGGACACCTGGTCGCGCCAGATTTTGCCTTGCCGCAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGCGCGTTGCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATCGGCGCGTTcgcc  <  2:120686/143‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                             
TTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGAGCGTGGCGACCTGGTCGCGCCAGATTTTGCCTTGCCGTAACGCCGCCAGCGCCTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATCGGCGCGTTCGCC  >  NC_000913/4126205‑4126481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑