Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,912 IS2 (+) +5 bp noncoding (14‑18/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3186096 =NA (NA)36 (1.340) 31/284 0.0 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4049916 0 (0.000)noncoding (18/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 4049912 =0 (0.000)26 (0.970) 23/284 0.2 100% noncoding (14/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region

GTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATC  >  NC_000913/4049778‑4050053
                                                                                                                                      |                                                                                                                                             
gtaagtaaTCAACATAAGCTGTAACGTTTTGTGATGGCTATTAGAAAGTCCTATGCAACAACTGAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagac                                                                                                                                      >  2:71906‑M2/1‑138 (MQ=255)
   gcgttcgagcattataacgaatggcatccgcctagtgcgctgggttgtcgctcgccacgggaatatctgcggcagcgggctcgtaatgggttaagtgataacagatgtctgggaatctaggggcaaatccaGTCATATGTTCt                                                                                                                                    <  2:41367‑M1/12‑1 (MQ=255)
       tcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtggtaacagatgtctggagaggtaggggcaactccaGTAAGCTGTCCtatgt                                                                                                                                <  2:382357‑M1/16‑3 (MQ=255)
        gCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggcccc                                                                                                                               <  1:279703‑M2/143‑13 (MQ=255)
          aaaaTAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccct                                                                                                                             >  1:416936‑M2/1‑129 (MQ=255)
           gcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCa                                                                                                                            >  1:155568‑M1/124‑143 (MQ=255)
              ttataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGAc                                                                                                                         >  1:3931‑M1/121‑143 (MQ=255)
                ataacgaatggcatccgcatagcgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCt                                                                                                                       >  1:244894‑M1/119‑143 (MQ=255)
                 gCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctc                                                                                                                      >  2:324302‑M2/1‑122 (MQ=255)
                  cTGTCACGTTTTGTGATGGCTATTAGACATTCCTATGCAACAACTGAAAAAAACTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggcccccagaatctcc                                                                                                                     <  2:384636‑M2/143‑23 (MQ=255)
                   cgtCACGTTTTGTCATGGCTATTAGAAATTCATATGCAACAACTGAAAAAAAATTACAAAAAGTGCTGTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctcca                                                                                                                    >  1:114389‑M2/2‑120 (MQ=255)
                    gTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccag                                                                                                                   >  1:191402‑M2/1‑119 (MQ=255)
                      ctcgtGTTGTGGTGGCTATTAGAAATTCATATGGAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagac                                                                                                                 >  2:233584‑M2/3‑117 (MQ=255)
                        cGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGACAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaa                                                                                                               >  2:9317‑M2/1‑115 (MQ=255)
                        cGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaa                                                                                                               <  1:257359‑M2/143‑29 (MQ=255)
                          ttttGGGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaacc                                                                                                             >  2:107446‑M2/1‑113 (MQ=255)
                          gcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttggaatgggttaagtgataacagatgtctggaaatataggggcaaatgcaGTAAGATGTTCTATCTTTCCGACCTGTGACTTCAc                                                                                                             <  2:459131‑M1/35‑1 (MQ=255)
                            ttGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaa                                                                                                           <  2:309226‑M2/143‑33 (MQ=255)
                                 aTGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatca                                                                                                      <  2:67878‑M2/143‑38 (MQ=255)
                                 aTGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAAATGAACAAAAAAGAGACAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggcaccctgagtctccatacaaccaatctca                                                                                                      <  2:461524‑M2/143‑38 (MQ=255)
                                   tagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGa                                                                                                    <  1:170738‑M1/44‑1 (MQ=255)
                                     gtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGAtt                                                                                                  >  2:482372‑M1/98‑143 (MQ=255)
                                     gtgcgcggggttatcgctcgccacgggaatagctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGAtt                                                                                                  >  1:130814‑M1/98‑143 (MQ=255)
                                      tATTAGGAGGTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaa                                                                                                 >  2:254415‑M2/1‑101 (MQ=255)
                                      tATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaa                                                                                                 <  1:11413‑M2/143‑43 (MQ=255)
                                       gcgctggattatcgcgcgccacgggaataactgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTg                                                                                                >  1:20665‑M1/96‑143 (MQ=255)
                                           gAAATCCCCACCCAACAACTGAGAAAAAATTACAAAAAGCGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataag                                                                                            >  1:430093‑M2/1‑96 (MQ=255)
                                            aaaaacctacacAACAACTGAAAAAAAAATACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagt                                                                                           >  1:23228‑M2/6‑95 (MQ=255)
                                             aaTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtg                                                                                          >  2:331466‑M2/1‑94 (MQ=255)
                                                  gcgctcgccaggggaatatcggcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATAttt                                                                                     >  2:424218‑M1/85‑143 (MQ=255)
                                                   tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAAGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtc                                                                                    >  1:459878‑M2/1‑88 (MQ=255)
                                                   tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAACTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaacatccagacaaccaatcacccttaaacaagcgacagtc                                                                                    <  2:154646‑M2/143‑56 (MQ=255)
                                                     ctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTtaa                                                                                  <  1:77602‑M1/62‑2 (MQ=255)
                                                         ccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatctaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgccgc                                                                              >  2:242403‑M1/78‑143 (MQ=255)
                                                          caacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatac                                                                             >  1:81852‑M2/1‑81 (MQ=255)
                                                                  aaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagttttt                                                                     >  1:156612‑M2/1‑73 (MQ=255)
                                                                  aaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagttttt                                                                     >  1:268971‑M2/1‑73 (MQ=255)
                                                                   atctgcggcagcgggcttgtaatgggttaagggataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtcagt                                                                    >  1:33593‑M1/68‑143 (MQ=255)
                                                                   agctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtcagt                                                                    >  1:287419‑M1/68‑143 (MQ=255)
                                                                        ttACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTATTGCGGTAAGtagactggccccctgaatctccagacaaccaatattcttttaataagtgatagtcttaatactagtttttagacta                                                               >  2:123800‑M2/1‑67 (MQ=255)
                                                                        ttACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagacta                                                               >  1:321124‑M2/1‑67 (MQ=255)
                                                                        ttACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagacta                                                               >  2:461085‑M2/1‑67 (MQ=255)
                                                                                ggcttgtaatgggtgaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCg                                                       >  1:78247‑M1/55‑143 (MQ=255)
                                                                                 gTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattgga                                                      >  2:211501‑M2/1‑58 (MQ=255)
                                                                                   ttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTCTCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAATCAGTAATGACTAGGGcgtat                                                    <  1:176435‑M1/92‑3 (MQ=255)
                                                                                   gCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggaga                                                    >  1:67878‑M2/1‑56 (MQ=255)
                                                                                          gggttaagtgataacagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTg                                             <  2:459149‑M1/99‑1 (MQ=255)
                                                                                           ggataagtgataacagatgtctggaaatataggcgtaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGTCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTCGGGCGTGTTTTACTgg                                            <  2:465334‑M1/100‑1 (MQ=255)
                                                                                                   aaacaaGAGTAAAGTTAGTAGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtc                                    >  1:157525‑M2/1‑40 (MQ=255)
                                                                                                        cagatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCCGTCAGTAATGACTGGGGCGGTTTTTATTGGGCgaaagaaaagg                               <  2:248713‑M1/113‑2 (MQ=255)
                                                                                                          gatgtctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATc                             >  2:170738‑M1/29‑143 (MQ=255)
                                                                                                            tAGAGTTAGTCGCGTAGGGTACAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccg                           >  2:25165‑M2/1‑31 (MQ=255)
                                                                                                              tctggaaatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTa                         <  2:90719‑M1/119‑1 (MQ=255)
                                                                                                                tggaaatataggggcaaatccaGTAAGATGTGCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTAAATAATGACTGGGGCGTTTGTAATTGGGCAAAAGAAAAGATCCGTAAt                       <  2:134797‑M1/121‑1 (MQ=255)
                                                                                                                   acatataggggcaaatccaGTAAGATGTTCTATCTTTCAGACCTTGTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTGCTGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAGTGcc                    <  2:317326‑M1/124‑1 (MQ=255)
                                                                                                                                actccaGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGt       >  1:155370‑M1/7‑143 (MQ=255)
                                                                                                                                 cAGAGGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttcgactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtacc      <  1:400736‑M2/143‑134 (MQ=255)
                                                                                                                                  agagGTAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggcgggagaaacgcggacggcggacca     <  1:53591‑M2/143‑135 (MQ=255)
                                                                                                                                     aGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATc  <  2:347845‑M1/142‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                             
GTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATC  >  NC_000913/4049778‑4050053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.