| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 61,461 | G→A | Q602* (CAG→TAG) | rapA ← | RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 61,461 | 0 | G | A | 95.5% | 57.8 / NA | 22 | Q602* (CAG→TAG) | rapA | RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (12/9); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGT > NC_000913/61326‑61602 | ataCGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATa < 2:199026/143‑1 (MQ=255) aaaTAGTGCCTCCGGTCCGTCAGGTGTGCTCAAATGCATCCAGACCCTCGTGGTACCACCCAACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATACGATCCAgacga < 1:90443/143‑1 (MQ=255) ggTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACAGACTGAGAGGTTTTCTCCAGATAAGGAACATGGATCTGAATAACGTGGGCCTAGCCGGTACGATCCAGACGACGAATACgctgct > 2:485665/1‑143 (MQ=255) tCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATCCGATCCAGACGACCAATACTCTGCTcc > 1:63218/1‑143 (MQ=255) cTCAACTGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGt < 2:267978/143‑1 (MQ=255) aTCCAGGCCGGCGTGATACCATGGCACCAGCACCGTCTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGTCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCa < 2:215652/143‑1 (MQ=255) ccAGACCTTCGTGATACCAGCGAACCAGCACCGACTGAGCGGGTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAgg < 2:322731/143‑1 (MQ=255) cAGACCTTCGTGATACCATCGCGCCAGCACCGACTGTGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATCTCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGt < 2:279387/143‑1 (MQ=255) aGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGGTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGCTACGATCCAGACGACCAATACGCTGCCGCAGGCGATCCGGGCTGAATGGCACGTc > 1:2107/1‑143 (MQ=255) cTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAAc > 1:145361/1‑143 (MQ=255) cAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGa > 1:340354/1‑143 (MQ=255) ccGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATACGATCCAGACGGCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTGAAACATCACCATGTGGCTGGCGAACTgg > 1:181693/1‑143 (MQ=255) gTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGGACTGGACGTTACGTcc > 1:275770/1‑143 (MQ=255) tttCTCCAGATAACGCACAAGGGTCGGAAGATCGCGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCtt < 1:455174/143‑1 (MQ=255) ccAGATAAGGCACATGGATCTGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGaa > 1:370187/1‑143 (MQ=255) ccgcaTGGCTCTGGTTATCGTGCGCCTAGCCGCTACTGTCCAGACGTCCTATACGCTGCTCCAGTACGTGCGAGTTGAAGGGCAATTTAATCTGCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCt < 2:422877/140‑1 (MQ=255) tCTGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGCTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACCGGAAGTTACGGCCTTCAGAACCGATTCCTGAGCAccgc > 2:243042/1‑143 (MQ=255) cTGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCAcagca < 1:186145/143‑1 (MQ=255) tGAATATCGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCAcagcag > 2:476900/1‑143 (MQ=255) cGTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCtgt > 1:161521/1‑143 (MQ=255) gTGCGCCTAGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCCGCACCGATTTCTGCGCTCCGCACTACAtgtg > 1:177953/1‑143 (MQ=255) tGGCCGCTACGCGCCAGTCGCCCAATCCGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGgt < 2:251696/143‑1 (MQ=255) | ATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGT > NC_000913/61326‑61602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |