Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC96.4% 82.5 / NA 28I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (16/11);  minor base G (1/0);  total (17/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926
                                                                                                                                         |                                                                                                                                           
tCTGATAGAGGGTAAACAGGTGATATTTGTGGCGGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  1:204435/143‑1 (MQ=255)
    ggagagGGGAAACCGCTGATAGCGTGAGCCGGCGCGGTCGAATTTGTCGGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                    <  1:295234/141‑1 (MQ=255)
        agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc                                                                                                                                <  1:483421/143‑1 (MQ=255)
         gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGTAGGGTGTGCGTCGCatct                                                                                                                               >  1:83982/1‑143 (MQ=255)
         gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctct                                                                                                                               >  1:321207/1‑143 (MQ=255)
         gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACACGGAGAATTTTTTCGGTGACGAGGTGATGTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGActct                                                                                                                               >  1:319881/1‑143 (MQ=255)
           gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc                                                                                                                             >  1:153279/1‑143 (MQ=255)
           gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc                                                                                                                             >  1:278595/1‑143 (MQ=255)
                    tGATATTTTTTGCTGGTGCTGTCGAATTTGTTTTTGTGCTTGCAGTAAAAGTGGGAGTACCCGGAGAAGTTGGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    <  1:113274/143‑1 (MQ=255)
                    ggATATTGTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    <  1:430680/142‑1 (MQ=255)
                      atatTTTTTGCTGGCGCGGTCTAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                                  <  1:95819/143‑1 (MQ=255)
                           ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTAGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTACTCTACTTTCCACGCTTCg                                                                                                             >  2:225585/1‑143 (MQ=255)
                                              tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATcc                                                                                          >  2:155132/1‑143 (MQ=255)
                                                   cTTTGTGCGTGCGGTAAGAGTGGAAGTACCCGGAGCATTTTTTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAgg                                                                                     <  1:164289/143‑1 (MQ=255)
                                                              cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCCTCTACTTTCCACGGTTCGCCGTGCTCCACCACCATCCGCAGGAAGATGTCATa                                                                          >  2:144469/1‑143 (MQ=255)
                                                                      gTGGAAGTCCCCGGAGAATCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  1:299822/143‑1 (MQ=255)
                                                                            gTACGCGGAGAATTTTTCCTGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCtt                                                            <  2:110375/143‑1 (MQ=255)
                                                                                      aaTTTTTTCGGTGACGAGGTGATCTGCATTTCGGCGAGATTGATATGCAGGCTCTGCGTCGCCACTTCTAGTTTCCACGTTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCacaccggg                                                  >  2:394276/1‑136 (MQ=255)
                                                                                               ggTGGCTAGGTGAGCTGCATTTCACCTTGATTGCTATGCAGGCTCTGCGTCGCTTCTTCTACTGTCCACTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                         <  2:40363/143‑1 (MQ=255)
                                                                                                           aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTCTCAGTGCGGTATCGAACAAACACTCTTTGaa                             >  2:384305/1‑143 (MQ=255)
                                                                                                            tCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                            >  1:161127/1‑143 (MQ=255)
                                                                                                                  tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAGCAGTCTTTGAACCGCCAt                      >  1:58064/1‑143 (MQ=255)
                                                                                                                  tttCACCGTGATTGATATGCAGGCTCTGAGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCAACACCCTCAGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAGCGAACAAACACTATTTGAAACGCCAt                      >  1:103886/1‑143 (MQ=255)
                                                                                                                       ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTGGCAACGCCATGCCCa                 >  1:421130/1‑143 (MQ=255)
                                                                                                                                  atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTACAGAGCt      >  1:306735/1‑143 (MQ=255)
                                                                                                                                   tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACCACCCCTCTTTGCAACGCCCTGCCCAGGTaaacgcccg     >  1:15124/1‑137 (MQ=255)
                                                                                                                                     cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGATCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACATGGt   >  2:207314/1‑143 (MQ=255)
                                                                                                                                      aGGCTCTGCGTCGCCTCTTCAACTATCCGCGGTTCGCCGTACTCTACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt  <  1:431590/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑