Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,445,981	T→G	31.7%	G34G (GGT→GGG)	ydbL →	DUF1318 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,445,981	0	T	G	31.7%	68.8 / 4.6	41	G34G (GGT→GGG)	ydbL	DUF1318 family protein
Reads supporting (aligned to +/- strand):  ref base T (4/22);  new base G (12/3);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 1.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATTGAAGTCGCTGCACCTAAGGAACCGATCACTATCAATATGAACGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTA‑GCTCGGGCC‑AAACCGGGGGAATATGTACAAGGGATAAACGGTAAATGGGTGCGAAAATTTTAA  >  NC_000913/1445744‑1446206                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     tgCATTTAAGTCGCTGCACCTAAGGAACCGATCACTATCAATATGAACGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTg                                                                                                                                                                                                                                     <  2:307187/238‑1 (MQ=255)    aTTTAAGTCGGTGCCACAAAGGAACCGGTCACTAAAAATATGAACGTTAAAATTGATGAAGAGTGCATCAACAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTTCTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGaa                                                                                                                                                                                                                                   <  2:639321/238‑1 (MQ=255)    aTTGAAGTCGCTGCACCTAAGGAACCGATCACTATCAATATGAACGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTCCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGG‑CGAAGGCACGAAATCAGGGGCGGGGAGGGGaaa                                                                                                                                                                                                                                  >  2:982377/1‑239 (MQ=255)                          aaCCGATCCCTATCAATATGAACGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGtt                                                                                                                                                                                                              <  1:388165/237‑1 (MQ=255)                           aCCGATCACTATCAATATGAACGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGGAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACGCAGGGGCGGGGAGGGGGAAAAATTTATGGGTATATGGtgtgg                                                                                                                                                                                                           >  2:214751/1‑235 (MQ=255)                           aCCGATCACTATCAATATGAACGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGGGAAACATTTTACGGTTATCTGGTTgc                                                                                                                                                                                                            >  1:607971/1‑238 (MQ=255)                                    tATCAATATGAACGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGGGAAACATTTTTCGGTTATCTGGTTGGGCTGAAAACg                                                                                                                                                                                                  >  1:1004951/1‑239 (MQ=255)                                        aaaatagcagTTAAAATTGGACAGGAGACAATCGTCAAGGCAGACAAAGATGGCGAAGAGCAGCTTGAAACTCGTAGCGATCTTGTCTGGGTTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTATGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGGGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATg                                                                                                                                                                                             <  2:316815/230‑1 (MQ=255)                                              aaCGTTAAAATTGAGCATGAGATAATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTCTGCGTTTCTTGTTGGGCTGGTAAGCCGCAATGTAATGGCATTGACTCTGGATGAAGCCG‑GAACTCAGGGGCGGGTAGGGGAAAAACTTTTCGGTTAACTGGTTTGCCTGGAAACGG‑ATGCGGaaa                                                                                                                                                                                       >  2:711774/1‑239 (MQ=255)                                               aCGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGaa                                                                                                                                                                                        <  1:711774/237‑1 (MQ=255)                                                cGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGGAGGGGAAACATTTTACGGTTTACTGGTTGCGCTGAAAACGG‑ATGCTggacaa                                                                                                                                                                                     >  1:405821/1‑234 (MQ=255)                                                cGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGGAGGGGAAAAATTTTTAGGTTGTTCGGTTGGGCTGGAAAAgggagtgggaaaa                                                                                                                                                                                      >  2:706041/1‑228 (MQ=255)                                                    aaaaTTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGGAGGGGAAACATTTTACGGGTATCTGGTTGCGCTGAAAACGG‑GTGCTGAAAAAaaga                                                                                                                                                                                 >  1:167212/1‑239 (MQ=255)                                                    aaaaTTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGGAGGGGAAAAAATTTTCGGTTTTTTGTTTTGGCTTAAAAAGGAATGCGGAAACagag                                                                                                                                                                                  >  1:757757/1‑239 (MQ=255)                                                       aTTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAACGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAAt                                                                                                                                                                              <  1:829931/239‑1 (MQ=255)                                                               tGAGATCATTAGTAAGGCAGACAAAGTTTTCGAAGGGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGGTCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGcc                                                                                                                                                                      <  2:58866/239‑1 (MQ=255)                                                                   atcatcatcAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTgcg                                                                                                                                                                                                           <  1:801057/199‑1 (MQ=255)                                                                           caAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTAAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCACTGTAATGGCATTGACTCTGGATGAAGCCA‑GCACTCAGGGGCGGGTAAGGGGAACATTTTTCTGTTATCTTGGGTCGCTGAAAAACG‑AAACAGAAAAAGAGAAAATAATAGCGGAAATAAAAGcg                                                                                                                                                          >  2:480307/1‑238 (MQ=255)                                                                            aaGGCAGGAAAAGATGTCGAAAGGCTGGTTTAAACTGTTAGCGATTTTTTCTGAGGTTATGATGAAGAAAACCTTTCTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCg                                                                                                                                                         <  2:1004951/239‑1 (MQ=255)                                                                                                  aggtgtttGAAACTCGTGGGGATTTTTTCTGAGGTGATGATAAAGAAAACATTACTTCTTTGTGCGTTTCTTGGTGGGCTGGTAAGCAGGAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACcaaca                                                                                                                                   <  2:167212/233‑1 (MQ=255)                                                                                                   gctgctTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCGGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAACCGACATTAATGCCGAAAGGAAAGCGGGTTACcaacaa                                                                                                                                  >  1:353836/1‑239 (MQ=255)                                                                                                         tGAAACTCTTAGCGTTCTTTTGTGAGGTGATGATGAAGAAAACATTTCTCCTTTGTGCGTTTCTTTGTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgca                                                                                                                            <  2:103028/239‑1 (MQ=255)                                                                                                         tGAAACTCGTAGCGATCTTTTCTGGGGTGATGATGAAGGAAACATTACTGCTTTGTGCGTTTCTTGGTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgca                                                                                                                            <  1:214751/239‑1 (MQ=255)                                                                                                                         tttgtatGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATgtgt                                                                                                            <  1:480307/233‑1 (MQ=255)                                                                                                                            ttCTGAGGGGATGATGAAGAAAACATTACTTCGTTGTGCGTTTCTGGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCGTGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAg                                                                                                                                            <  1:275808/204‑1 (MQ=255)                                                                                                                                   gTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACa                                                                                                                                                                                     <  1:247039/156‑1 (MQ=255)                                                                                                                                   gTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGGTGCGCTGAAAACGG‑ATGCTGAAACa                                                                                                                                                                                     >  2:247039/1‑156 (MQ=255)                                                                                                                                                          tttttGTGCGTTTCTGGGTGGGCTGGTAAGGAGAAATGTAATGGCCTTGTCTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGGGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCaaa                                                                           <  2:757757/237‑1 (MQ=255)                                                                                                                                                          ttcttGTGCGTTTCTTGTTAGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCaaa                                                                           <  1:1830/236‑1 (MQ=255)                                                                                                                                                             ttGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGGGAAACATTTTTCGGTTTTCTGGTTGCGCTGGAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAACGAGTTACCAACAACCGGCCAAGCACAATAATGTGTCGGTAGATGGTATCGCGAAACTCGCCCGGCaaaa                                                                          >  1:546244/1‑237 (MQ=255)                                                                                                                                                                                   gaaaGCAGCATTGTAATGGCATTGACTCGGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTA‑GCTCGGGCC‑AAACCggg                                                <  2:353836/237‑1 (MQ=255)                                                                                                                                                                                    gaagggacaATTTTATGGATTTGGCTCTGGATGGAGGCG‑GAACGCAGGGGGGGGTAGGGTAACAATTTTACCGTTAGCTGGTTGGTCTGAAAACGG‑AAGCTGAAACAGAGAAATTAGTAGCCGAAATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTA‑GCTCGGGCC‑AAACCggg                                                <  2:546244/231‑1 (MQ=255)                                                                                                                                                                                     cccaggcaATTTAATGGCCTTGACTCTGGATGAAGCCA‑GAACTCAGGGGGGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTA‑GCTCGGGCC‑AAACCggg                                                <  1:247332/232‑1 (MQ=255)                                                                                                                                                                                        cataaaTGAAATGGCATTGACCCTGAATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAATTTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTA‑GCTCGGGCC‑AAACCGGGGGAat                                           <  1:556521/235‑1 (MQ=255)                                                                                                                                                                                             aTGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑AAGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAACTCGTA‑GACCGGGCC‑AAACCGGGGGGATATGtt                                      >  1:831189/1‑238 (MQ=255)                                                                                                                                                                                                tAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCg                                                                     <  1:592636/207‑1 (MQ=255)                                                                                                                                                                                                       aTTTACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGGAGGTGAAACTTTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTA‑GCTCGGGTC‑AAACCGGGGGAATATGTACAAGGGATa                             <  2:831189/238‑1 (MQ=255)                                                                                                                                                                                                          gACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGGGAAACATTTTTCGGTTATCTGGTTGCGCTGAAAACGG‑GTGCTGAAACAGAGAAATTAGTAGCCGGCATTAATGCCGAAAGTAAAGGGAGTTACCAACAACTGGGAAAGCAAAATAATGTGTCGGGAGAAGATATCCCGGAAACCGCCGGGCAAAAACACGTAGGCCGGGGCA‑AAAACGGGGGAA‑ATGTACAAggggaaaaaa                         >  2:606436/1‑232 (MQ=255)                                                                                                                                                                                                                             aaCTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTAcc                                                                                                                                       <  1:1012720/113‑1 (MQ=255)                                                                                                                                                                                                                                tCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTA‑GCTCGGGCC‑AAACCGGGGGAATATGTACAAGGGATAAACGGTAAATGGGTGCGAAAAtttt    <  1:982377/239‑1 (MQ=255)                                                                                                                                                                                                                                  aGGGGCGGGGAGGTGAAAAATTTTACGGGTATCTGGGTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGAAGCCGACATTAATGCCGAACGGAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGAGGATATCGCGCAACTCGCCGGGCAAAAACACGTA‑GCCCGGGCCAAAACCGGGGGAA‑ATGTAAAAAGGAGAAAAGGGAAAGGGGGGGGGAAAATTTaa  >  2:615564/1‑239 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     CGCATTGAAGTCGCTGCACCTAAGGAACCGATCACTATCAATATGAACGTTAAAATTGAGCATGAGATCATCATCAAGGCAGACAAAGATGTCGAAGAGCTGCTTGAAACTCGTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCA‑GAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGG‑ATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTA‑GCTCGGGCC‑AAACCGGGGGAATATGTACAAGGGATAAACGGTAAATGGGTGCGAAAATTTTAA  >  NC_000913/1445744‑1446206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑