| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,335,254 | T→C | 12.7% | E414G (GAG→GGG) | murA ← | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,335,254 | 0 | T | C | 12.7% | 173.1 / 4.0 | 77 | E414G (GAG→GGG) | murA | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (26/39); new base C (10/2); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.69e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCTGGCGAATGCATTATCGCGCCCATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCGTGCGCGCCCATACGGCTCAGCTCTGGC > NC_000913/3335017‑3335490 | acgtaggCCAATGCATTAGCGGGCCCATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTc < 1:210183/234‑1 (MQ=255) gCGAATGCATTATCGCGCCCATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCCCGAACGGGGCTGCCCGCCCTCAGACGATTATTCGCCTTTCACACC‑CCCAATAtt > 1:963293/1‑239 (MQ=255) ggAATGCATTATCGCGCCCATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATAtt < 2:206548/237‑1 (MQ=255) aaggcctattCGCCCCCCTGGCCGAAAGGAGTGGGGATTATTGCGAAAGCACTGGGAGCTGATCCCGGGGTTATCTGGCCATCACGCTACCATGCTCCGCAGAGTCAAGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGAGGATTATTCGCCTTTCACACG‑CTCAATATTTg < 2:469472/229‑1 (MQ=255) cATTATCGCGCCCATGGCCGAAAGGGGATATGATTATTGCGAAAGCCCTGGGAACCGACCCCTGGGTTATCTGGCCATCACGGTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CCCa < 2:488406/227‑1 (MQ=255) gcgcCCATGGGCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAgcgc < 1:838843/239‑1 (MQ=255) cggccATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAgcgcg < 1:324157/236‑1 (MQ=255) cccATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACGGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGCACGGGGCTGCCCGCCCTCCGACGATTATTCGCCTTTCACCCG‑CCCAATATTTGCCCCCAAAgccgcca > 1:990002/1‑234 (MQ=255) cccATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGCAGATTATTCGCCTTTCACACC‑CCCAATATTTGCCCCCAAa‑cgcgcca > 1:596667/1‑237 (MQ=255) cccATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTAAACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGGACGGGGCTGCCAGCCCTCAGACGAGTATTCCCCTTTTACACG‑CCCCAAATTTGCAccctggcccttat > 1:488406/1‑228 (MQ=255) ggCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTg < 1:413763/239‑1 (MQ=255) aaGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAACTGAATGGTCTGGCGGTAGCCCCGCGACCGGGGCTCGCAGCTCTCCGACCATTATTCGCCTTTCCCCCA‑CCCAAAATTTTCACCTAAAACCCGCCGTTTTTTTTCa > 2:1018565/1‑239 (MQ=255) aaGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CCCACAATTTGCACCTAAAACCCCCAGTTTGTCTTCa > 1:332720/1‑239 (MQ=255) aGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTc < 1:688916/237‑1 (MQ=255) gagaTGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTCATCTGGCCATCACGCTACCATGATCCGCATACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGTCGGTAGCCCAGCGAACCGGGCTGCCAGCTCTAAGGACATTATTCGCCTTGCACACA‑CCCCACACTTTCACCCAAAGCGCCCAGGTCGTCCTAAAcgcg > 2:170465/1‑239 (MQ=255) ttgtttttGCGAAAGCCCTGGGAACCGACCCCTGGTTTATCTGGCCATCCCGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAG‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCg < 2:175580/233‑1 (MQ=255) attattGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCCGCTCTCAGACGCTTATTCGCCTTTCCCACG‑CCCAATATTTTCACCCAAAGCGCGCCGTTTGTCTTCCATGCGTTCGtc > 2:688916/1‑238 (MQ=255) acacgCGAAAGCCCGGGGAACTGCCCCCTGGGTTATCTGGCCATCACGCTTCCATGAACCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTa < 2:273313/233‑1 (MQ=255) cccaaGCCCGTGGAACTGCACCCTGGGTTATTGGGCCCTCCCGCCTACATGATCCCCAGACCCATGAGTTTATCGGCAGAACGCAGGTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcga < 2:919210/236‑1 (MQ=255) aaGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCCTGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcgatcg < 1:679978/239‑1 (MQ=255) ccccTGGAGACTGTCCCCTGGGTTCTCTGGCCATCCCTGTACCATGGTCCGCAGACCCATGAGTTTAACGACAGAACGCAGTGTAT‑GCGTAGCTACAGTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCGGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcgatcgat < 2:305673/238‑1 (MQ=255) ggAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcgatcg > 1:273313/1‑231 (MQ=255) cccTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAgcgcgc < 1:189063/189‑1 (MQ=255) tCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACAGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAAAATTTGACCCTAAAGCTCCCAGTTTGTCTTCATGTCTTTCGTACCCACGATCGATGTGTTAAACACGATCAaccccccg > 2:854304/1‑236 (MQ=255) tCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCCCGAACGGGGCTGCCAGCTCTCAGACGATTTTTCGCCTTTCCCCCG‑CTCAATATTTTCACCCAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCCCCATCGCTTGGTTAAAAACAATCAACAACcgcc > 2:186790/1‑237 (MQ=255) ccATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTACAGCCCGCAGTTTGTCTTCAATGCGTTTCTAGCCACGCTCGATGTTATAAATACGATCAAACACcctccgcc > 2:608864/1‑234 (MQ=255) cctCACGCTACCATGGCCCCCCGACCAATGAGGTGATCGACAGAACGCAGTTGATGGCGT‑GCTACACTAAACCGAAAAAATGAGGGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTccc < 2:596667/236‑1 (MQ=255) aCGCTACCATGATCCGGATGCCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGAtgtg < 1:700977/207‑1 (MQ=255) tACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGTTAAATACg > 1:246801/1‑212 (MQ=255) tACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACg < 2:246801/212‑1 (MQ=255) tCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCCATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGGTAAATACGATCAACCACCCTCCGCCCTTCCGCAATAcagcca > 1:319021/1‑238 (MQ=255) ccGCAGACCCATGAGTTTAACGAAAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccagc < 1:1018565/239‑1 (MQ=255) cGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccagcc < 1:724400/239‑1 (MQ=255) cAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAgcgcgc < 1:800712/153‑1 (MQ=255) cAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAgcgcgc > 2:800712/1‑153 (MQ=255) cAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcacccagcccg > 1:283120/1‑237 (MQ=255) cAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCCCCTTTCACACG‑CTCAACATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCCCGAACGATGTGATAAATACGTCCAACCACCGCCGGCCCTTCCGCACTAcagccaccca > 1:757971/1‑238 (MQ=255) cAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGGAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CCCCAAATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAAAACGATCAACCACCGTCGTCCCTTCCCCAATCaagccagcca > 1:801076/1‑238 (MQ=255) cAGACCCATGAGTTTATCGACAGAACGCAGATGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGTAAACCCCGTGAACGGGGCTGCCAGCTCTCAGACGATAATTCGCCTTTCACACG‑CTCAATATTTGAACCTAAAACGCGCAGTTTGTCTTCAATGCGTTCg > 1:844624/1‑174 (MQ=255) tGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCCATACAACCAGCCAGCACCaa > 1:542720/1‑236 (MQ=255) ttATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTg < 1:925150/237‑1 (MQ=255) aTCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTCAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAACTACGATCAACCACCGCCGTCCCTTCCGCAATACAGCCAGCCAGAACCAGGCTTGCTGa > 1:242186/1‑239 (MQ=255) aTCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAgcgc < 2:452791/136‑1 (MQ=255) aTCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGa > 1:213406/1‑239 (MQ=255) aTCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAAAATTTGCACCTAAAgcgc > 1:452791/1‑136 (MQ=255) aCAGAACGCATTTGAT‑GGGTAGCCTCCCTAAACCGAAAAAATAGGTGGTCTGGGGGTAGCCCCGGAGACGGGGCTGCCAGCGCGCAAACGGTTATTCGCCTTTCACACG‑CTCAATATTTGCTCCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCa < 2:963293/239‑1 (MQ=255) cgcattgttt‑GCGTGCCTACACTAAACCGAAAAAATTGGTGGTCGGGCGGTAGCCCCGCGAACGGGGCGTCCAGCCTCCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCAACTAAAGCGCTCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGa < 2:990002/230‑1 (MQ=255) ttGGT‑GCGGGGCGACCCTAACCCGAAAAAATGAGGGGTCTGGGGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACCCG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCgg < 2:903649/238‑1 (MQ=255) ttGAG‑GCGTAGTGACCCTAACCCGAAAAAATGAGTGGTCTGGCTGTAGCCCCGCTAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATAATTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCATGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCgg < 1:170465/238‑1 (MQ=255) aT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCCGCCCCAGGCTTGCTGATACCCCCAGATCGGGTg > 1:385782/1‑238 (MQ=255) tAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATAtt < 1:428325/99‑1 (MQ=255) tAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATAtt > 2:428325/1‑99 (MQ=255) cccTAAACCGAAAAAATGGGGTGTTTGGGGGTAGCCCCGGGAACGGGGCTGCCAGCTCTCAGACGATTTTTCGCCTTTCACCCG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCtgtg < 2:213406/237‑1 (MQ=255) aaCCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCTTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGccccc > 1:786445/1‑238 (MQ=255) aaCCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCAAACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGAACAACCACCGCCGTCCCTTCCGCAATACATCCAGCCAGCACCAGGCTTGCTGTTGCCCGCAGATCGGGTGCCCTAACCTTTGCGCCa > 1:674300/1‑239 (MQ=255) aaCCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTAGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGATTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATAAACCACCGTCGTCCCTTCCGCAATACAGCCCGCCAGCACCAGGCTTTCTTAGGCCCGCCAAACAGGTGCCACAACCtgggacgca > 2:108457/1‑232 (MQ=255) ccGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCCTGCTGATGCACGCAGATCCGTTGCCATAACCTGTGCCCCAgg > 1:832747/1‑238 (MQ=255) aaaaaaTGTGGTGTCTTGCGGTCGCCCCGCGAACGGGGTTGCCAGCTCTCAGGCGAGTTGTCGCCTGTCACCCT‑CTCAATAGTTGCACCTAAAGCGCGCAGTGTTTCTTCAATTCTTTCGTAGCCACGATCGATGTGATAAATACGATCCACCTCCGTCGTCCCTTCCGCTATTCAGCCAGCCAGCACCACGCTTGCTGATGCACGCAGGTCGGTTGCCATAGCCTCTGCGCCAGaaac < 2:674300/239‑2 (MQ=255) aGTGGTCTGGCGGTACCCCCGGGAACGGGGCTTCCAGCTCTCAGACGGTTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGACGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCaa < 2:332720/239‑1 (MQ=255) tGGCCTGGCGGTAGCCCCACGAACGGGGCTGCCAGCTTATAAACGTTTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGAAGTTTGTCTTCACTGCCTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAaca < 1:981849/239‑1 (MQ=255) gggttggggggTGGCCCCGGCAACGGGGGTGCCAGCTCTCAGCCGATTATTCGCCTTTCCCACG‑CTCAAGATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCTAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAaca < 2:385782/230‑1 (MQ=255) tGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTg < 1:608864/239‑1 (MQ=255) gCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGCTAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCCGGCTTGCTGAAGCACCCAAATCGGTTGCCATAACCTTGGCGCCAGAAAGCTTTTCAACACCGTGACaaaaaa > 2:887729/1‑239 (MQ=255) gCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCCATCCAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCCAAACCTGTGCGCCCAAAAGGTTTTCAAAACCCTGACAAATaa > 2:946654/1‑239 (MQ=255) gCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcag < 1:276869/154‑1 (MQ=255) gCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CCCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCCCGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATAaag > 2:276869/1‑154 (MQ=255) cgAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATaaa > 1:466069/1‑194 (MQ=255) ggCTGCCAGCTCTCAGACGATTATTCGCCTTTCAACCG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCttt < 1:696458/238‑1 (MQ=255) tctcAGGCGTTTTTTTGCCTTTCGCCCGTCCAAATATTGACA‑CTAACGCGCCGCGTTGGTCTTCAATGCGTTCGTAGCCACGAACGATGGTATAAATACGAACACCCACCGTCGTCCCTGCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCg < 2:242186/236‑1 (MQ=255) tctcAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCTCCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTAATGCACGCAGACCGGTGCCCATACCCTGTTCCCCAGAAGGTTTTTCAACACCTCGCCAAATAACGGAATTGTTTTCAATTTCGGCg > 2:334766/1‑239 (MQ=255) tcAGGCGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCGTg < 1:108457/239‑1 (MQ=255) aGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCGTgc > 1:541147/1‑238 (MQ=255) cGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCGTgcgc < 1:887729/237‑1 (MQ=255) cGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCGTgcgc < 1:186790/237‑1 (MQ=255) ttcgcccTTTCCCCCG‑CTCAAATTTGGCCCCTAAAGCGCGCAGTTTGTCTGCAATGCGTTCGTAGCCGCGATCGATGTGCTAAAGACGATCAACCACCGTCGTCCCTTCCCCAAGTCAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCGTGCGCGCCCATa < 2:801076/234‑1 (MQ=255) ccTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGGCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCGTGCGCGCCCATACGGCt < 1:946654/239‑1 (MQ=255) cTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGTTGCACGCAGATCGGTTGCCATACCCTGTCAGCCAGAAAGTTTTTCAACACCGTGCCAGATACCGGAATTGCTTTCGATTTCGGCGTGCCCGCCCCTACGGCCCAGctcctgg > 1:739590/1‑234 (MQ=255) | ACGCTGGCGAATGCATTATCGCGCCCATGGCCGAAAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGAT‑GCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACG‑CTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCGTGCGCGCCCATACGGCTCAGCTCTGGC > NC_000913/3335017‑3335490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |