| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,061,965 | A→G | 35.1% | E56G (GAG→GGG) | torD → | TorA‑maturation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,061,965 | 0 | A | G | 35.1% | 20.1 / 3.3 | 24 | E56G (GAG→GGG) | torD | TorA‑maturation chaperone |
| Reads supporting (aligned to +/- strand): ref base A (12/3); new base G (6/2); total (19/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
GCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTA‑GTTGAGGCAGGGAT‑GGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCT > NC_000913/1061763‑1062196 | gcAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGGGAACGGGCT‑GGAAAACCGTATTGCCCCGCTGCCAGTACGGGa > 1:891201/1‑239 (MQ=255) tGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCCGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCCGTGCGCAGATGGCTGAATGGGTTTAGTTGCT‑GAAAAGCGAACCGCCGCTAACTGCGGCGGTGGAAGAGTTGGGAAAACCG‑ATTTCCAGGCTGAAAGTagggacacat > 2:119704/1‑230 (MQ=255) cGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGGGAACGGGCT‑GGAAAACCGTATTGCACCGCTGACAGTACGTGACGATg > 1:147298/1‑238 (MQ=255) ttCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGGTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGGGAACGGGCT‑GGAAAACCGGATTGCCACCCCGACAAGACGGGACGGAGCCCGGCT‑gg > 2:487869/1‑239 (MQ=255) tCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGCCGGTTAACTGGCC‑GGGAAACCGGATTGCCCCGCTGACCACACGGGACGGT‑CCCCTCTGGGAACTggac > 1:466762/1‑237 (MQ=255) gCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGGGCT‑GGAAAACCGTATTGCCACGCCGCCAGTACGTGACGATGCCCCTCT‑GGAACTGGcccc > 1:669569/1‑237 (MQ=255) caacaGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGGCT‑GGAACTGGCCCCGGGCTTTTGGGGCCCGTTTCTGATGACCg > 1:687337/1‑238 (MQ=255) aTTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGCCTTTTTCGGCCTGTTTTTTATGACCGccaaaaa > 2:985442/1‑237 (MQ=255) tGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGGAAACCGTATTGCCACGCTGACAGGACGTGACGATGCCCGTCT‑GTAACTGGCCGCGGGCTTTTTGGGCCCGTTTTTGATGACGCacaaaaaaa > 2:137095/1‑238 (MQ=255) tGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAAACGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGGCTTTTTCGGCCTGTTTCTGATGACCGacaaacaaa > 1:107763/1‑238 (MQ=255) tgtTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGGAAACCGTATTGCCACGCTGACAGACCGTGACGATGCCCGTCT‑GGGACTGGCCGCGGACTTTTGCGGCCGGTTTCTCATAACCGCCAAAAAAACGgggc > 2:138051/1‑239 (MQ=255) gCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTgg > 1:877964/1‑179 (MQ=255) cgcAGTTGTTATCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGGGAAAGAGCT‑GGAAAACCGGATTGCCACGCTGAACAAAAGTGACGAGGCCCGTCT‑GGAAATGGACGCGGGCTTTTGCGTCCCGGTTTGGAAGCCCGAGAAAAAAAGGGGGCGGCGGTATACAGCGGc > 2:917383/1‑238 (MQ=255) gttgttCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGGATTGcc > 1:678805/1‑133 (MQ=255) ttgttCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGa > 1:948912/1‑119 (MQ=255) aaacaagTGACGAAACCCCCCAGGGCGAAG‑TGGGTGAATTGGTTTTGTTGGTAAAAAAGCAACCCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACg < 1:138051/238‑1 (MQ=255) actccccAGGGGGCAGGTGGCTGATTGGTTTTCGTTGCG‑AAAAAGGCAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTA‑GTTGAgg < 1:137095/234‑1 (MQ=255) aaTCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACCGGCT‑GGAAAACCGGATTTCCACGCTGACAGTACGTGACGAATCCCGGCT‑GGAACTGGCCCCGGCCTTTTGCGGCCTGTTTTTCATGACCGCCAAACAACCCGCGCTCGCGTATGCCACGGCCCACAAAACAGACGAGCAACAGATTAAACCCTttttgaggtggg > 2:975677/1‑231 (MQ=255) cAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGAGTAAACCCTTGTTA‑GTTGAGGCAGGGGT‑GGgaa > 1:985951/1‑238 (MQ=255) cT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAAATTGCT‑CGAAAACCGTATTGCCACGCTGTCCGAACGTGAGGCTGACCCTCT‑GGAACGTGCCGCGGCCTTTTTAGGCCCGTTTCTGCTGAACGGTCAACAAACGGCGTCGCCGTATTGACCGGCCTTCAAACAGGATGAGACAAAGATTAAAAACCTGCTATGTTG‑GGCAGGGGTGGGAAACCA‑CGGCACTTTTAAAGaaacac > 2:992451/1‑234 (MQ=255) ctgcccagtGCGGCGGTTAACGAGGT‑GGAAAACCGTATAGCCAAGCTGACAGTAGGTGACGATGCCCGGCT‑GGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGAAAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTA‑GTTGAGGCAGGGAT‑GGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGat < 1:998142/231‑1 (MQ=255) cACTGCGGCGGTGAACGGGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTA‑GTTGAGGCAGGGAT‑GGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGatctatc < 1:1853/239‑1 (MQ=255) ggcggTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTA‑GTTGAGGCAGGGAT‑GGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGAACAATCTCGAAt > 1:318791/1‑239 (MQ=255) ggAACGGGCT‑GGAAAACCGTATGGCCACGCGGACAGTACGTGACGATGCCCGTCT‑GGAACGGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTA‑GTTGAGGCAGGGAT‑GGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCt < 1:883842/237‑1 (MQ=255) | GCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCT‑GAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCT‑GGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCT‑GGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTA‑GTTGAGGCAGGGAT‑GGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCT > NC_000913/1061763‑1062196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |