Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G100.0% 89.1 / NA 34intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/16);  total (18/16)

GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG  >  NC_000913/3560316‑3560587
                                                                                                                                            |                                                                                                                                    
gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGTCACTTTGCGGGCGATGCGCTTTTTTTTTTCTTTCTTTGTTGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgca                                                                                                                                    <  1:531456/143‑4 (MQ=255)
      cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGCAAGGCAGCGACGCACCGACATGATGGCGCAGGATCa                                                                                                                              >  1:106328/1‑143 (MQ=255)
        gCGTCGTGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGGTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAgg                                                                                                                            <  2:601697/143‑1 (MQ=255)
                    ttggtaTTTGCTCCGCCGCGTTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGACCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCATAGAACTAAAATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                <  2:398029/138‑1 (MQ=255)
                       ggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCTCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                             >  1:364886/1‑143 (MQ=255)
                         aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGCTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCGGCCCCGCCATGAGGGCGCAGGATTAGGTTTTGCTCCgtcggcgc                                                                                                           >  2:332049/1‑136 (MQ=255)
                               tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACAAAGAAAACAGCGCCGCACCGCCATGATGGCGCAGGATCCGGATATGCACAGCCAGCTCATTGAg                                                                                                     >  2:250390/1‑143 (MQ=255)
                                  gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCGACCTCAATGAgctg                                                                                                  >  1:609150/1‑140 (MQ=255)
                                       tttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACTGAACTGGAAGGCAGCGACGCACCGCCATCATGGCGCAGGATCAGGTTTTGCTCCGCCAGGTCATGGAGGCCgcggc                                                                                             >  2:673481/1‑143 (MQ=255)
                                            gggCTAGGCGGTCTTTTGCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                        <  2:663789/143‑1 (MQ=255)
                                                       tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAACTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                             >  1:346643/1‑143 (MQ=255)
                                                       tctTTTTCTTCGGTCTGGGTGACCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCCCCACCGCCATGATGGCGCAGGATAAGGTTTTGCTCCGCCAGCCAAGTGACGACGCAGCGACTAGTCAGCAGGca                                                                             >  2:260710/1‑142 (MQ=255)
                                                              cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTGTCGCTCCGCCAGCTCATTGAGGTCGGGGCGAATAGTCTGCGGGCTGACGgag                                                                      >  2:617614/1‑143 (MQ=255)
                                                               ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGgaga                                                                     >  2:693270/1‑143 (MQ=255)
                                                                    tCTGGGTGGCCTGGCGATCGGGCGACGGGGTGTTGACCGCACGGGAAGGCAGCGCCGCGCCGGCATGATGGCGGAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                <  2:653192/143‑1 (MQ=255)
                                                                         gTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                           <  1:592602/143‑1 (MQ=255)
                                                                         gTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                           <  1:590786/143‑1 (MQ=255)
                                                                           ggCCTTGTGTTCGTGCCACGGTGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCATGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCa                                                         <  2:229223/143‑1 (MQ=255)
                                                                            tccgcgCGATCGTGCGCCGGCGGGTTCACCGAACGGGATGGCAGCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCATACTGACGGATAAATGCTCTACCAg                                                        <  2:441252/138‑1 (MQ=255)
                                                                               ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGGGGGCTGACGGAGAACTGCTCTAcacgccc                                                     >  2:342674/1‑137 (MQ=255)
                                                                               ttGCGATCGTACCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCGCTACCAGcta                                                     >  2:128996/1‑142 (MQ=255)
                                                                                 gCGATCGTGCCACGGGGTGTTAACCGGACTGGAAGGCAGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGACATGCTCTACCAGCTCtt                                                   <  2:258658/143‑1 (MQ=255)
                                                                                  cGATCGTGCCACGGCGTGTTAGCCGTACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                  <  2:562481/143‑1 (MQ=255)
                                                                                   gATCGTGCGACTGCGCGTTAACCGAACTGGTAGGCAGTGCCGCGCCGCCATGATGGCGCAGGAGCAGGGTTGGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                 <  1:221177/143‑1 (MQ=255)
                                                                                      cGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGACATGCTCTACCAGCTCTCCGGTa                                              >  2:76332/1‑143 (MQ=255)
                                                                                        tGCCATGGCGTGTGGACGGAACTGGTAGGCAGCTCTGCACCTCCGTGATGGCGCAGGATCAGGTTTTGCTACGCCAGCTCGTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt                                            <  2:645382/143‑1 (MQ=255)
                                                                                           cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                         >  1:463482/1‑143 (MQ=255)
                                                                                               acGTGTTAACCGAACTGCAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCCGGTTTTGCGCCGCCACCTCATTGAGGTCGCGGAGAATAGTATGCGGGCTGACGGAGAAATCCTCTAGCAGCTCTTCGCTACTGACATaa                                     >  2:349240/2‑143 (MQ=255)
                                                                                                    ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGGTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                >  2:181157/1‑143 (MQ=255)
                                                                                                        cctaaCTGGAGGTCTGCGCTGCACCGCTATGATGTCGTAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGACGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                            <  1:263054/140‑1 (MQ=255)
                                                                                                             cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGCTCAGGTTTCGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                       <  1:313381/143‑1 (MQ=255)
                                                                                                                gAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAACGCTCTACCAGCTCTTCGGTACTAACATAACACTGGTATTTAATCAg                    >  2:704936/1‑143 (MQ=255)
                                                                                                                     cAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTTGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa               <  2:352987/143‑1 (MQ=255)
                                                                                                                             cagcGCCATGTTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg       <  2:95689/140‑1 (MQ=255)
                                                                                                                                  ccATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg  >  1:171514/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                    
GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG  >  NC_000913/3560316‑3560587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: